Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the plant genotype on root-associated methanotrophic structures, especially on active flora, remain poorly understood. Transcription of the pmo A gene, encoding particulate methane monooxygenase in methanotrophs, was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from root samples of three emergent macrophytes, including Phragmites australis , Typha angustifolia , and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland. High-throughput sequencing of pmo A based on DNA and cDNA was used to analyze the methanotrophic community. Sequencing of cDNA pmo A amplicons confirmed that the structure of active methanotrophic was not always consistent with DNA. A type I methanotroph, Methylomonas , was the most active group in P. australis , whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter . In T. angustifolia , these two types of methanotroph existed in similar proportions. However, at the DNA level, Methylomonas was predominant in the roots of all three plants. In addition, vegetation type could have a profound impact on root-associated methanotrophic community at both DNA and cDNA levels. These results indicate that members of the genera Methylomonas (type I) and Methylocystis (type II) can significantly contribute to aerobic methane oxidation in a eutrophic wetland. Key points 1. Root-associated Methylomonas was predominant in three macrophytes using DNA approach. 2. Active Methylocystis was dominant in genera Typha and Schoenoplectus , but not in Phragmites . 3. Plant species impact on methanotrophic communities in both DNA and cDNA levels.