The relationship between Visceral Adiposity Index and estimated Pulse Wave Velocity: Insights from NHANES Database

The relationship between Visceral Adiposity Index and estimated Pulse Wave Velocity: Insights from NHANES Database | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The relationship between Visceral Adiposity Index and estimated Pulse Wave Velocity: Insights from NHANES Database Yanwei Liu, Shiqiang Yang, Jun Wei This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5660240/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 9 You are reading this latest preprint version Abstract Background Obesity and arterial stiffness are known risk factors for cardiovascular disease, but the relationship between visceral adiposity index (VAI) and estimated pulse wave velocity (ePWV) is still unclear. Methods The cross-sectional study used data from the 2007–2016 survey cycle of the National Health and Nutrition Examination Survey (NHANES ) database. After data cleaning, the researchers conducted a comprehensive weighted statistical analysis of the final data set. This included data on demographics, medical history, tests, examinations and chronic comorbidities. The researchers used restricted cubic spline curves to examine potential linear associations between VAI and ePWV. In addition, weighted linear univariate and multivariate regression analyses were performed to further explore the potential relationship between VAI and ePWV. Finally, multiple subgroup analyses and interaction tests, as well as sensitivity analyses, were performed to test the stability of the relationship. Results Finally, 10458 adult participants aged 20 years or older were included in this study, with a mean (SD) age of 49.2 ± 17.4 years after weighted analysis. Restricted cubic spline analysis showed a potentially "inverted-L" non-linear relationship between VAI and ePWV ( P for non-linearity: <0.001).The inflection point analysis suggests its inflection point is (1.48: 1.38–1.57). Linear multivariate regression analysis suggested a significant positive correlation between VAI (0.2SD) and ePWV values (β = 0.1, 95% CI 0.04–0.17, p < 0.001). The positive correlation between VAI (0.2SD) and ePWV levels remained stable in model analyses adjusted for all covariates. And in the highest quartile Q4 group, it showed a significant positive correlation compared to Q1 (β = 0.53, 95% CI 0.41–0.64, p < 0.001). These findings remained stable and consistent in subsequent subgroup and sensitivity analyses. Conclusions According to this study, elevated VAI levels are related to estimated pulse wave velocity, and there is an interaction between VAI and ePWV in populations that are aging, hypertensive, and diabetic. obesity arterial stiffness visceral adiposity index estimated pulse wave velocity NHANES Figures Figure 1 Figure 2 Figure 3 Figure 4 Introduction The global burden of cardiovascular disease has increased significantly as a result of economic growth and an ageing population[ 1 ]. A loss of arterial elasticity with age is often observed. Progressive stiffening of the vessel wall is an inherent physiological feature associated with vascular aging[ 2 ]. However, this feature is exacerbated by ageing and chronic non-communicable diseases. As an important marker of vascular ageing, arterial stiffness is important in the assessment of vascular health[ 3 ]. It is also associated with the development of cardiac, cerebrovascular and diabetic diseases. A growing number of studies have confirmed the potential positive association between the degree of atherosclerosis and these diseases[ 4 ]. Studies of arterial stiffness are currently a hot topic in the field. The current study has confirmed the diagnostic role of arterial stiffness in the assessment of cardiovascular disease risk [ 5 ]. Carotid-femoral pulse wave velocity (cf-PWV) is considered the gold standard for assessing aortic stiffness [ 6 ]. Despite the non-invasive nature of (cf-PWV) and brachial-ankle pulse wave velocity (baPWV) measurements. However, the clinical use of this method is currently extremely limited due to the need for specialised equipment [ 7 ]. Estimated pulse wave velocity (ePWV) is a new index based on mean blood pressure (MBP) and age that has now been shown to be a reliable predictor of atherosclerosis.Although estimated PWV is not a complete replacement for cf-PWV, recent clinical studies have demonstrated its ability to independently predict cardiovascular disease events and survival [ 8 , 9 ]. Obesity has been identified as a global epidemiologic challenge and its prevalence continues to rise despite several public health measures currently in place worldwide [ 10 ]. Recent studies have shown that visceral adipose tissue (VAT) is a more reliable indicator of obesity than total body fat mass [ 11 ]. There is a potential link between visceral obesity and a variety of conditions, including vascular aging, carotid atherosclerosis, metabolic disorders, and increased all-cause mortality [ 12 , 13 ]. However, imaging techniques such as computed tomography (CT) and magnetic resonance imaging (MRI) are costly, time-consuming, and radiation intensive. There are still major obstacles to universal clinical application. VAI, calculated from waist circumference, body mass index (BMI), triglyceride levels, and high-density lipoprotein cholesterol (HDL-C), has been identified as a gender-specific index. Current studies have demonstrated that VAI is a reliable predictor of a variety of disease states, particularly associated with obesity [ 14 , 15 ].VAI is a novel index for the assessment of obesity that integrates a variety of metabolic indicators. It can reflect the distribution of visceral fat and metabolic status more comprehensively. ePWV is an important indicator for assessing arterial stiffness. ePWV is closely related to metabolic health and the onset and progression of cardiovascular disease. However, there are relatively few studies on the relationship between VAI and ePWV. The purpose of this study was to analyze the potential relationship between VAI and ePWV using data from a national survey. Methods Study design and population in NHANES This study is based on data from NHANES, a US national survey conducted from 2007 to 2016 using a complex stratified multistage probability sampling design to ensure national representation. The NHANES study protocol was approved by the Research Ethics Review Board of the National Center for Health Statistics, and written informed consent was obtained from all participants.The study report was prepared in accordance with the Enhanced Guidelines for Reporting Observational Studies in Epidemiology. An ethical exemption was granted by the Academic Review Board of the First People's Hospital of Yibin, as the raw data used in this study were obtained from publicly available databases.This study analyzed data from five NHANES survey cycles (2007–2008, 2009–2010, 2011–2012, 2013–2014 and 2015–2016). And the final analysis data were obtained after strict inclusion and exclusion criteria. The final analysis included 10,458 individuals. ( The flow chart is shown in Fig. 1 ). Assessment of visceral adiposity index For men and women, VAI was calculated using two separate equations: for men, VAI = (WC/[39.68 + 1.88 × BMI)] × (TG/1.03) × (1.31/HDL), and for women VAI = [WC/(36.58 + 1.89 × BMI)] × (TG/0.81) × (1.52/HDL), where BMI is body mass index (kg/m2), WC is waist circumference (cm), TG is triglycerides (mmol/L), and HDL is high-density lipoprotein in mmol/L[ 14 ]. VAI normalization process In the subsequent analyses of this study, we standardized the visceral adiposity index (VAI) to 0.2 standard deviations (SD) to improve the interpretability of the model and the stability of the results. Specifically, we calculated the mean and standard deviation of the VAI and divided each participant's VAI value minus the mean by 0.2 times the standard deviation. This standardization method is very common in epidemiological and biostatistical studies, especially when dealing with variables with different scales. With this treatment, the regression coefficient can be interpreted as the effect of each 0.2 standard deviation increase in VAI on estimated pulse wave velocity (ePWV). Assessment of ePWV The Arterial Stiffness Reference Values Collaborative Group formulae, as described by Greve et al, were used to calculate ePWV using age (years) and mean blood pressure (MBP, mmHg),as follows: ePWV = 9.587 − 0.402 × age + 4.560 × 10 − 3 × age 2 -2.621 × 10 − 5 × age 2 ×MBP + 3.176 × 10 − 3 ×age ×MBP-1.832 ×10 − 2 × MBP. The formula for MBP is DBP plus 0.4 times the difference between SBP and DBP[ 16 ]. Blood pressure was recorded by a trained health technician, with participants having three consecutive measurements after 5 minutes of quiet sitting. Covariables Data Extraction The selection of covariates was based on previous reports in the literature and clinical experience.The covariates included several demographic factors such as age (in years), sex (categorised as male and female), ethnicity, marital status, poverty income ratio (PIR) and education level.The laboratory results included triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL), glycosylated haemoglobin and fasting blood glucose. Dietary information was collected from two 24-hour recall interviews (midnight to midnight) in which participants reported the types and amounts of food and beverages (including all types of water) consumed in the 24 hours prior to the interview, along with an estimate of energy, protein, carbohydrate, fibre, total fat, and sugar intakes, and information on hypertension, diabetes, coronary heart disease, and stroke. Detailed descriptions of the study variables are available on the official CDC website: www.cdc.gov/nchs/nhanes/.Diagnostic criteria for diabetes mellitus included an official diagnosis by a physician, use of glucose-lowering medication or insulin, a glycated haemoglobin (HbA1c) level of 6.5% or higher, a fasting blood glucose level of 7.0 mmol/L or higher, or a blood glucose level of 11.1 mmol/L or higher on a random blood glucose test or 2-hour oral glucose tolerance test. Hypertension was characterised by an average systolic blood pressure of 140 mmHg or higher and an average diastolic blood pressure of 90 mmHg or higher, as diagnosed by a physician or treated with antihypertensive medication. Weights processing To ensure the national representativeness of the study results, we processed sample weights in strict accordance with official NHANES guidelines. As this study integrated NHANES data from five cycles from 2007 to 2016, we used the following weight processing steps:1. weight standardization: sample weights for each cycle were standardized using the formula: standardized weight = original weight/mean of weights for that cycle. 2. weight merging: the standardized weights were merged into a single unified weight using the formula: merged weight = standardized weight/number of cycles. 3. weighted analyses: all statistical analyses were performed using the merged weights to ensure that the results reflected the true picture of the US national population. Statistical Analysis and sensitivity analysis The sample was weighted and stratified according to NHANES guidelines, and the NHANES study population was categorised into four different groups based on VAI quartiles. Categorical data were presented as frequencies (expressed as percentages), while continuous variables were presented using the mean and standard deviation if the distribution was normal, and the median and interquartile range (IQR) for skewed distributions. Comparative analyses were performed using one-way analysis of variance (ANOVA) for continuous variables with normal distributions, the Kruskal-Wallis H test for continuous variables with skewed distributions, and the chi-squared test for categorical variables to assess statistical significance between different groups. Prior to linear regression analysis, we performed an initial test of the distribution of the dependent variable (estimated pulse wave velocity, ePWV). Although the original distribution of ePWV may not be exactly normal, the key assumption of the linear regression model is that the residuals should be approximately normal. Therefore, the distribution of the residuals was tested as follows: histograms of the residuals were plotted and the Shapiro-Wilk normality test was performed. The results show that the distribution of the residuals is close to normal and the p-value of the Shapiro-Wilk test is greater than 0.05, indicating that there is not enough evidence to reject the hypothesis that the residuals are normally distributed. We also attempted to log-transform the ePWV to improve the normality of its distribution, but ultimately decided to use the original ePWV values for analysis because the interpretation of the log-transformed values was not as intuitive as the original values. We confirmed the appropriateness of using the raw ePWV values for linear regression analysis using residual tests. In this investigation, potential confounding variables were first identified through a screening process, and then a series of statistical analyses were performed to explore the relationship between VAI and ePWV.In this study, the restricted cubic spline method was used to model the exposure-outcome relationship. Where potential non-linear correlations were detected, inflection point analyses were performed to identify inflection points where the relationship between VAI and ePWV changed significantly.Univariate and multivariate linear regression analyses and model analyses with stepwise adjustment for covariates were then performed to further explore the potential relationship between VAI and ePWV. For modelling analyses, we compared VAI when it was used as a continuous or categorical variable. To assess the relationship between VAI and ePWV, we used weighted multivariate linear regression analyses with stepwise adjustment for multiple potential confounders. We developed four different models with progressively more adjusted variables. Model 1 did not adjust for any confounders and assessed only the crude correlation between VAI and ePWV; model 2 adjusted for sex and race; model 3 further adjusted for education level and PIR based on model 2; and model 4 further adjusted for other confounders such as hypertension, diabetes mellitus, cardiovascular disease, SBP, DBP, cigarette smoking and alcohol consumption based on model 3. In addition, we examined the interactions between the main confounders and VAI and performed several sensitivity analyses to ensure the robustness of the results. We also performed detailed diagnostic analyses of the final model, including residual analysis, linear hypothesis testing, and multiple covariance testing, to ensure the applicability of the model and the reliability of the results. For the statistical computations, the Free Statistics software version 2.0 (Beijing, China) and the R programming environment version 4.3.2 were utilized. The p-values reported are two-tailed, with a threshold of less than 0.05 set to determine statistical significance, thereby allowing for the identification of associations that are unlikely to occur by chance. Results Study population This study analyzed data from five NHANES survey cycles (2007-2008, 2009-2010, 2011-2012, 2013-2014, and 2015-2016), initially excluding participants younger than 20 years (n=21,387) from the total of 50,588 individuals. We then excluded participants with missing systolic blood pressure data (n=4129), diastolic blood pressure data (n=63), waist circumference data (n=1142), body mass index data (n=55), triglyceride data (n=12911), and other variables (n=443).With strict exclusion, the missing covariate data for the remaining participants was less than 5%. As a result, multiple interpolation was used to cleanse the study, resulting in the inclusion of 10458 subjects in the final analysis( Figure 1 ). Characteristics of the participants All participants were divided into four groups according to baseline VAI quartiles. As shown in Table 1, group Q1 (VAI: 2.41, N=2633). Data on demographic characteristics, dietary intake, examination data, laboratory data, and questionnaires for all participants in each subgroup are shown in Table 1 . Univariate and Multivariate Linear Regression Analyses In this study, univariate regression analysis between VAI and various covariates and ePWV was systematically analysed using linear regression analysis. The results indicated a possible positive association between VAI and ePWV (β: 0.1; [95% CI: 0.04,0.17], P =0.003). In addition, univariate analyses performed with all covariates for exposure suggested statistically significant differences between age, male, race, education, PIR and energy intake and outcomes (all P <0.001). Detailed results are presented in Supplementary Table 1 . Covariance tests for covariates were then performed using methods such as variance inflation factor (VIF). The results indicated a significant colinearity between energy intake, carbohydrate intake, total sugar intake, total fat intake, BMI, WC, TC, TG, HDL-c and LDL-c. Detailed results are presented in Supplementary Table 2 . Covariates that were significantly colinearity were removed in subsequent multivariate analyses. In the multivariate linear regression analysis, we found a potential positive association between VAI and ePWV. This association remained significant after adjustment for multiple confounders.In model 1, no adjustment for covariates was made and the results indicated a possible positive correlation between VAI and ePWV (β: 0.1; [95% CI: 0.04,0.17], P =0.003). Stable positive associations were also observed in models with stepwise adjustment for covariates. In model 4, adjusted for all covariates, the results were suggestive (β: 0.09; [95% CI: 0.02,0.16], P =0.007). All participants were then divided into four different groups according to the quartiles of the VAI. The lowest group (Q1, VAI < 0.87) was used as the reference group. Linear multivariate regression analysis continued with four different models. No covariate adjustment was performed in model 1. Groups Q2 (β: 0.31; [95% CI: 0.19,0.43], P <0.001), Q3 (β: 0.54; [95% CI: 0.42,0.65], P <0.001) and Q4 (β: 0.62; [95% CI: 0.50,0.74], P <0.001) showed a progressively higher positive correlation. The results remained stable and consistent in model 4 adjusted for all covariates. The results remained stable and consistent in models with stepwise adjustment for covariates. In model 4 adjusted for all covariates, the results were Q2 (β: 0.27; [95% CI: 0.16,0.38], P <0.001), Q3 (β: 0.49; [95% CI: 0.37,0.60], P <0.001) and Q4 (β: 0.53; [95% CI: 0.41,0.64], P <0.001). Detailed results are presented in Table 2 . We also tested for interactions between the main confounders and VAI and found no significant interactions. The results of the sensitivity analysis were consistent with those of the main analysis, further validating the robustness of the results. Model diagnostic analysis showed that the residual distribution was close to normal, the linearity assumption was valid, and no significant multicollinearity problems were found. Restricted cubic spline models Restricted cubic spline models were constructed to fit the curve between VAI and ePWV. Smoothed curve fits between variables are indicated by the solid red line, and shaded bands indicate 95% confidence intervals. Adjustment for covariates revealed a non-linear relationship between VAI values and ePWV (p for non-linearity <0.001) (Figure 2) . Inflection point analysis was then performed and the results suggested a significant positive correlation between VAI and ePWV when VAI was less than 1.48 (1.38-1.57) (β:0.49; [95% CI: 0.31,0.67], P <0.001). However, there was no statistically significant correlation between the two when VAI exceeded 1.48 (β:0.06; [95% CI: -0.01,0.12], p= 0.09)( Supplementary Table3 and Supplementary Figure1 ). To further validate the stability of the findings, we performed RCS analysis after stratifying the population. An "inverted L-shaped" non-linear relationship, consistent with the whole population, was observed in the male, female and age 60 years, hypertension and diabetes strata showed higher levels of VAI and ePWV than the total population ( Supplementary Table 4 ). Potential negative linear associations were found in stratified RCS analyses( Figure 3 ). Subgroup analysis and Sensitivity analysis To further validate the potential relationship between VAI and ePWV, we also analysed participants in subgroups based on age, sex, race, education, history of hypertension, history of diabetes, and history of CVD ( Figure 4 ). When VAI was subgrouped as a continuous and categorical variable, the results were consistent with the overall results. Significant interactions were found in the subgroups of age >60 years, hypertension, and diabetes ( Supplementary Table 5 and Supplementary Table 6 ). Discussion In this cross-sectional study, we examined the relationship between the VAI, a novel indicator of adiposity distribution and dysfunction, and ePWV, a marker of arterial stiffness, in a representative sample of US adults. Our results showed a significant positive association between VAI and ePWV, particularly below a VAI inflection point of 1.48. This association remained robust after adjustment for various confounders and was consistent across subgroup analyses, except for potential interactions within the age > 60 years, hypertension, and diabetes subgroups. These findings suggest that abdominal obesity, as quantified by VAI, may be an important contributor to atherosclerosis. This study provides valuable insights into the complex interplay between obesity and vascular health and highlights the need for further research to develop targeted interventions to mitigate the impact of abdominal obesity on arterial stiffness and reduce cardiovascular risk. Arterial stiffness, which progresses with age, results in thickening of the arterial walls and narrowing of the lumen [ 17 ]. Arterial stiffness is largely determined by the severity of ischaemia in the affected organ, with most patients showing few clinical signs in the early stages.Research has shown that arterial stiffness is associated with an increased risk of cardiovascular disease, stroke and other diseases, yet a sensitive and specific laboratory diagnostic method for early arterial stiffness is lacking[ 18 ]. As cf-PWV has not been widely used in clinical practice, ePWV has played a role in raising awareness of the risks of aortic stiffness and assisting physicians in its clinical application[ 19 ].The study involved using a previously published formula to assess ePWV and investigate its correlation with visceral adiposity index.Some recent research suggests a positive association between body fat levels and arterial stiffness, while other studies suggest a negative or no association [ 20 ].A study from the United States, which used VFA to accurately measure visceral fat to assess obesity, showed that the normal weight visceral obese group was associated with arterial stiffness and increased 10-year cardiovascular risk [ 21 ].A study from Spain, which also used body mass index, ratio and BMI to assess obesity, showed that indicators of obesity were inversely correlated with arterial stiffness, as measured by the cardio-ankle vascular index (CAVI) and brachial-ankle pulse wave velocity[ 22 ]. A study from Japan showed that measures of obesity, such as fat volume index, body mass index and body circumference index, were positively correlated with brachial-ankle PWV [ 23 ]. In a study of Korean adults, ePWV was found to correlate more significantly with waist circumference than with BMI, supporting the concept that visceral obesity is more closely associated with arterial stiffness than total obesity [ 24 ].The results of our study regarding the association between visceral adiposity index and vascular stiffness are consistent with this. Therefore, our study offers the following advantages: The first notable advantage is that ePWV, as a marker of arterial stiffness, is more accessible and easily reproducible in clinical practice; the second important feature is that VAI, as a measure of visceral adipose function, is a sex-specific index that includes simple anthropometric and common epidemiological parameters, and this calculated value provides an association between abdominal obesity and arterial stiffness; Thirdly, the study we conducted this time had a sufficiently large sample size; and fourthly, for clinical purposes, this association provides a simple, valid and comprehensive assessment of cardiovascular disease in primary prevention populations and even in Stage 0 prevention groups, which is crucial for reducing the global burden of cardiovascular events. Future clinical guidelines and public health policies on obesity and cardiovascular health may be influenced by the results of this study. A number of underlying mechanisms have been proposed to elucidate the initiation and progression of obesity and arterial stiffness.On the one hand, visceral adiposity actively secretes large amounts of inflammatory mediators, which can lead to increased insulin resistance, enhanced oxidative stress and endothelial cell dysfunction, all of which induce structural changes in the arterial wall and thus promote arterial stiffness[ 25 ]. A critical element to consider is the role of adipokines, bioactive peptides with endocrine activity released from visceral adipose tissue. For example, leptin, an adipokine, is thought to activate the sympathetic nervous system. This activation can lead to increased blood pressure and increased arterial stiffness[ 26 ]. On the other hand, levels of lipocalin, which has anti-inflammatory and anti-atherosclerotic properties, tend to be reduced in individuals with visceral obesity [ 27 ].It is also noteworthy that visceral adipose tissue actively expresses and releases components of the renin-angiotensin system, such as angiotensinogen and angiotensin II. The involvement of this system in the development of hypertension and vascular remodelling is well established, and both play an important role in atherosclerosis [ 28 ].In addition, abdominal obesity and atherosclerosis share common risk factors, including hypertension, diabetes, dyslipidaemia and sedentary lifestyle.It is conceivable that these diseases, while increasing atherosclerosis, may also have feedback mechanisms that indirectly contribute to abdominal obesity [ 29 , 30 ]. In the present study, we found a significant positive correlation between VAI and ePWV. However, the basal level of ePWV was significantly higher than the total population level in the subgroups of patients older than 60 years, hypertensive patients and diabetic patients( Supplementary Table 7 ). An interaction between VAI and ePWV was observed in these subgroups. This phenomenon suggests that obesity seems to have an "inverse effect" on atherosclerosis in these specific subgroups. The higher the degree of VAI, the less pronounced the increase in ePWV compared to the total population. This phenomenon may be related to the interaction of several pathophysiological mechanisms associated with VAI . The relationship between visceral obesity and arterial stiffness is not a simple linear one. Visceral obesity has been shown to be strongly associated with increased arterial stiffness.VAT secretes a variety of inflammatory factors and adipokines, such as leptin and lipocalin. These factors contribute to increased arterial stiffness by affecting insulin resistance, oxidative stress and endothelial cell function, promoting structural changes in the arterial wall [ 31 ] However, in specific populations, visceral obesity may have a complex effect on arterial stiffness through other mechanisms.In the subgroup aged &amp;gt;60 years, ePWV levels were significantly higher than in the total population. This is consistent with previous studies showing that atherosclerosis increases with age [ 32 ]. However, the positive correlation between VAI and ePWV was attenuated and even reversed in this subgroup. This phenomenon may be related to the pathophysiological mechanisms of atherosclerosis in the elderly population. With age, the ratio of collagen to elastin in the arterial wall changes, leading to increased arterial stiffness [ 33 ]. In this context, obesity may have a "buffering" effect on arterial stiffness by affecting the metabolic and inflammatory state of the vascular wall. In addition, there is a high prevalence of comorbid chronic diseases (e.g. hypertension, hyperuricemia,diabetes mellitus) in the elderly population, and these diseases may themselves exacerbate arterial stiffness, thus masking the direct effect of obesity on arterial stiffness [ 34 , 35 ].In the hypertensive subgroup, ePWV values were also significantly higher than in the total population. Hypertension is a major risk factor for increased atherosclerosis. It can lead to structural remodelling of the arterial wall and increased stiffness by increasing blood flow shear and vessel wall pressure [ 36 ]. In this subgroup, the interaction between VAI and ePWV may be related to the effect of obesity on blood pressure regulation. Obese patients tend to have hyperactivation of the sympathetic nervous system, which may further exacerbate elevated blood pressure and increased arterial stiffness in hypertensive patients [ 37 ]. However, obesity may also have a complex effect on arterial stiffness in hypertensive patients by affecting the renin-angiotensin-aldosterone system (RAAS) and vascular endothelial function. On the one hand, obesity may exacerbate arterial stiffness by increasing RAAS activation; on the other hand, obesity may have a protective effect on arterial stiffness by affecting vascular endothelial function [ 38 ].In the diabetic subgroup, ePWV values were significantly higher than in the total population, which is consistent with previous studies indicating the presence of higher arterial stiffness in diabetic patients [ 39 ]. Patients with diabetes tend to have insulin resistance, hyperglycaemia and a chronic inflammatory state. Together, these factors lead to increased glycosylation and oxidative stress in the arterial wall, exacerbating atherosclerosis [ 40 ]. In this subgroup, the interaction between VAI and ePWV may be related to the effect of obesity on the metabolic status of diabetic patients. Obese patients tend to have more severe insulin resistance, which may further exacerbate elevated blood glucose and increased arterial stiffness in diabetic patients [ 41 ]. However, obesity may also have some protective effect on atherosclerosis by affecting lipid metabolism and inflammatory status in diabetic patients. For example, obese patients tend to have higher levels of high-density lipoprotein cholesterol (HDL-C), which may have a protective effect on oxidative stress and the inflammatory state of the arterial wall . Limitations We acknowledge several limitations of our study. First, in the cross-sectional analyses, we excluded data with missing exposures and outcomes, which may introduce selection bias.Second, some of the data were collected by questionnaire, which may have introduced significant recall bias. Despite accounting for various confounding factors, there may still be other yet-to-be-discovered factors that could influence the results of the study and thus affect the validity of the findings.Further rigorously designed clinical trials are needed to investigate the potential association between visceral adiposity index and ePWV. In addition, the cross-sectional design of our study did not allow us to establish a causal relationship between VAI and ePWV. Conclusions In this cross-sectional study, a significant positive association was observed between visceral adiposity index and estimated pulse wave velocity in a representative sample of US adults, particularly below a VAI inflection point of 1.48. This association remained robust after adjustment for various confounders and was consistent across subgroup analyses, highlighting the potential role of abdominal obesity in atherosclerosis and the importance of addressing this issue to reduce cardiovascular risk. Further research is warranted to explore interventions that may mitigate the effects of abdominal obesity on atherosclerosis. Declarations Author Contributors The authors of this work have satisfied the ICMJE criteria for authorship and have significantly contributed to the manuscript through tasks such as data analysis and interpretation, drafting, critical revision, and final approval. The study was designed by S.Y. and Y.L., while Y.L. authored the manuscript. S.Y. collected all clinical data. S.Y. and J.W. statistically analyzed clinical data. The report was thoroughly reviewed and edited by all authors, who subsequently granted their approval for the final draft. Funding This study was supported by Medical Research Program of Yibin Municipal Health Commission (2024YW001). Acknowledgement We thank all the researchers and participants involved in NHANES databases. We thanks Dr. Jie Liu (People’s Liberation Army of China General Hospital, Beijing, China) for helping with research design. We thank the Science and Technology Programme of Yibin Municipal Health Commission for supporting this study. Data availability statement All the raw data required in this study can be extracted from the NHANES online website. For further inquiries, please contact the first author or corresponding author. Clinical trial number: Not applicable Competing interests The authors declare no competing interests. Ethics statement The current study used publicly available summary data, and ethical review and approval for research involving human participants have been conducted in accordance with local legislative and institutional requirements for preliminary studies in which data were collected. Therefore, written informed consent was not required for this study, and no additional ethical approval was needed. ORCID Yanwei Liu https://orcid.org/0009-0006-1096-2862 Shiqiang Yang https://orcid.org/0000-0003-3138-1777 References Wang H, Yu X, Guo J, et al. Burden of cardiovascular disease among the Western Pacific region and its association with human resources for health, 1990-2021: a systematic analysis of the Global Burden of Disease Study 2021. Lancet Reg Health West Pac. 2024;51:101195. Published 2024 Sep 3. doi:10.1016/j.lanwpc.2024.101195 Lee J, Song RJ, Musa Yola I, et al. Association of Estimated Cardiorespiratory Fitness in Midlife With Cardiometabolic Outcomes and Mortality. 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Burden of disease attributable to high body mass index: an analysis of data from the Global Burden of Disease Study 2021 [published correction appears in EClinicalMedicine. 2024 Nov 21;78:102958. doi: 10.1016/j.eclinm.2024.102958]. EClinicalMedicine. 2024;76:102848. Published 2024 Sep 24. doi:10.1016/j.eclinm.2024.102848 Zou X, Zhou X, Li Y, et al. Gender-specific data-driven adiposity subtypes using deep-learning-based abdominal CT segmentation. Obesity (Silver Spring). 2023;31(6):1600-1609. doi:10.1002/oby.23741 Yaskolka Meir A, Keller M, Bernhart SH, et al. Lifestyle weight-loss intervention may attenuate methylation aging: the CENTRAL MRI randomized controlled trial. Clin Epigenetics. 2021;13(1):48. Published 2021 Mar 4. doi:10.1186/s13148-021-01038-0 Liu C, Pan H, Kong F, et al. Association of arterial stiffness with all-cause and cause-specific mortality in the diabetic population: A national cohort study. Front Endocrinol (Lausanne). 2023;14:1145914. Published 2023 Mar 8. doi:10.3389/fendo.2023.1145914 Amato MC, Giordano C, Galia M, et al. Visceral Adiposity Index: a reliable indicator of visceral fat function associated with cardiometabolic risk. Diabetes Care. 2010;33(4):920-922. doi:10.2337/dc09-1825 Zierfuss B, Höbaus C, Herz CT, Pesau G, Koppensteiner R, Schernthaner GH. Predictive power of novel and established obesity indices for outcome in PAD during a five-year follow-up. Nutr Metab Cardiovasc Dis. 2020;30(7):1179-1187. doi:10.1016/j.numecd.2020.03.019 Greve SV, Laurent S, Olsen MH. Estimated Pulse Wave Velocity Calculated from Age and Mean Arterial Blood Pressure. Pulse (Basel). 2017;4(4):175-179. doi:10.1159/000453073 d'El-Rei J, Cunha MR, Mattos SS, et al. Microvascular Reactivity in Hypertensive Patients with High Body Adiposity. Estudo da Reatividade Microvascular em Pacientes Hipertensos com Adiposidade Corporal Elevada. Arq Bras Cardiol. 2020;115(5):896-904. doi:10.36660/abc.20190364 Ataee Z, Aghaee A, Sobhani SR, et al. Evaluation of Arterial Stiffness and Its Relation to Innovative Anthropometric Indices in Persian Adults. Int J Hypertens. 2023;2023:2180923. Published 2023 Jan 23. doi:10.1155/2023/2180923 Vishram-Nielsen JKK, Laurent S, Nilsson PM, et al. Does Estimated Pulse Wave Velocity Add Prognostic Information?: MORGAM Prospective Cohort Project. Hypertension. 2020;75(6):1420-1428. doi:10.1161/HYPERTENSIONAHA.119.14088 Gong J, Han Y, Gao G, et al. Sex-specific difference in the relationship between body fat percentage and arterial stiffness: Results from Fuzhou study. J Clin Hypertens (Greenwich). 2023;25(3):286-294. doi:10.1111/jch.14649 Zheng L, Sun A, Han S, et al. Association between visceral obesity and 10-year risk of first atherosclerotic cardiovascular diseases events among American adults: National Health and Nutrition Examination Survey. Front Cardiovasc Med. 2023;10:1249401. Published 2023 Aug 21. doi:10.3389/fcvm.2023.1249401 Saz-Lara A, Cavero-Redondo I, Moreno-Herráiz N, et al. Association between body shape index and arterial stiffness: results of the EVasCu study and a meta-analysis. Int J Obes (Lond). Published online October 28, 2024. doi:10.1038/s41366-024-01663-8 Yoshida Y, Nakanishi K, Jin Z, et al. Association Between Progression of Arterial Stiffness and Left Ventricular Remodeling in a Community-Based Cohort. JACC Adv. 2023;2(5):100409. Published 2023 Jul 5. doi:10.1016/j.jacadv.2023.100409 Kim HL, Joh HS, Lim WH, et al. Associations of Estimated Pulse Wave Velocity with Body Mass Index and Waist Circumference among General Korean Adults. Metabolites. 2023;13(10):1082. Published 2023 Oct 15. doi:10.3390/metabo13101082 Ardissino M, McCracken C, Bard A, et al. Pericardial adiposity is independently linked to adverse cardiovascular phenotypes: a CMR study of 42 598 UK Biobank participants. Eur Heart J Cardiovasc Imaging. 2022;23(11):1471-1481. doi:10.1093/ehjci/jeac101 Engin A. Endothelial Dysfunction in Obesity and Therapeutic Targets. Adv Exp Med Biol. 2024;1460:489-538. doi:10.1007/978-3-031-63657-8_17 Opdebeeck B, Huysmans I, Van den Branden A, Orriss IR, D'Haese PC, Verhulst A. Deletion of the P2Y2 receptor aggravates internal elastic lamina calcification in chronic kidney disease mice through upregulation of alkaline phosphatase and lipocalin-2 [published correction appears in FASEB J. 2023 Aug;37(8):e23069. doi: 10.1096/fsb2.23069]. FASEB J. 2023;37(1):e22701. doi:10.1096/fj.202201044R Takeda Y, Demura M, Yoneda T, Takeda Y. DNA Methylation of the Angiotensinogen Gene, AGT, and the Aldosterone Synthase Gene, CYP11B2 in Cardiovascular Diseases. Int J Mol Sci. 2021;22(9):4587. Published 2021 Apr 27. doi:10.3390/ijms22094587 Zheng J, Hu Y, Xu H, et al. Normal-weight visceral obesity promotes a higher 10-year atherosclerotic cardiovascular disease risk in patients with type 2 diabetes mellitus-a multicenter study in China. Cardiovasc Diabetol. 2023;22(1):137. Published 2023 Jun 12. doi:10.1186/s12933-023-01876-7 West HW, Siddique M, Williams MC, et al. Deep-Learning for Epicardial Adipose Tissue Assessment With Computed Tomography: Implications for Cardiovascular Risk Prediction. JACC Cardiovasc Imaging. 2023;16(6):800-816. doi:10.1016/j.jcmg.2022.11.018 Engin A. Endothelial Dysfunction in Obesity and Therapeutic Targets. Adv Exp Med Biol. 2024;1460:489-538. doi: 10.1007/978-3-031-63657-8_17. Vishram-Nielsen JKK, Laurent S, Nilsson PM, et al. Does Estimated Pulse Wave Velocity Add Prognostic Information?: MORGAM Prospective Cohort Project. Hypertension. 2020;75(6):1420-1428. doi:10.1161/HYPERTENSIONAHA.119.14088 Marshall AG, Neikirk K, Afolabi J, et al. Update on the Use of Pulse Wave Velocity to Measure Age-Related Vascular Changes. Curr Hypertens Rep. 2024;26(3):131-140. doi:10.1007/s11906-023-01285-x Ardissino M, McCracken C, Bard A, et al. Pericardial adiposity is independently linked to adverse cardiovascular phenotypes: a CMR study of 42 598 UK Biobank participants. Eur Heart J Cardiovasc Imaging. 2022;23(11):1471-1481. doi:10.1093/ehjci/jeac101 Cassano V, Crescibene D, Hribal ML, et al. Uric Acid and Vascular Damage in Essential Hypertension: Role of Insulin Resistance. Nutrients. 2020;12(9):2509. Published 2020 Aug 19. doi:10.3390/nu12092509 d'El-Rei J, Cunha MR, Mattos SS, et al. Microvascular Reactivity in Hypertensive Patients with High Body Adiposity. Estudo da Reatividade Microvascular em Pacientes Hipertensos com Adiposidade Corporal Elevada. Arq Bras Cardiol. 2020;115(5):896-904. doi:10.36660/abc.20190364 Gong J, Han Y, Gao G, et al. Sex-specific difference in the relationship between body fat percentage and arterial stiffness: Results from Fuzhou study. J Clin Hypertens (Greenwich). 2023;25(3):286-294. doi:10.1111/jch.14649 Opdebeeck B, Huysmans I, Van den Branden A, Orriss IR, D'Haese PC, Verhulst A. Deletion of the P2Y2 receptor aggravates internal elastic lamina calcification in chronic kidney disease mice through upregulation of alkaline phosphatase and lipocalin-2 [published correction appears in FASEB J. 2023 Aug;37(8):e23069. doi: 10.1096/fsb2.23069.]. FASEB J. 2023;37(1):e22701. doi:10.1096/fj.202201044R Liu C, Pan H, Kong F, et al. Association of arterial stiffness with all-cause and cause-specific mortality in the diabetic population: A national cohort study. Front Endocrinol (Lausanne). 2023;14:1145914. Published 2023 Mar 8. doi:10.3389/fendo.2023.1145914 Zheng J, Hu Y, Xu H, et al. Normal-weight visceral obesity promotes a higher 10-year atherosclerotic cardiovascular disease risk in patients with type 2 diabetes mellitus-a multicenter study in China. Cardiovasc Diabetol. 2023;22(1):137. Published 2023 Jun 12. doi:10.1186/s12933-023-01876-7 Zou X, Zhou X, Li Y, et al. Gender-specific data-driven adiposity subtypes using deep-learning-based abdominal CT segmentation. Obesity (Silver Spring). 2023;31(6):1600-1609. doi:10.1002/oby.23741 Tables Table 1 Baseline characteristics of participants in NHANES (N = 10458). Variables Total (n = 10458) Quartile1 (n = 2575) Quartile2 (n = 2642) Quartile3 (n = 2608) Quartile4 (n = 2633) p Demographics Data Gender, n (%) < 0.001 Male 5192 (49.6) 1368 (53.1) 1292 (48.9) 1254 (48.1) 1278 (48.5) Female 5266 (50.4) 1207 (46.9) 1350 (51.1) 1354 (51.9) 1355 (51.5) Age,year 49.2 ± 17.4 46.1 ± 17.8 48.7 ± 17.8 50.8 ± 17.3 51.3 ± 16.3 < 0.001 Race/Ethnicity, n (%) < 0.001 Mexican American 1658 (15.9) 258 (10) 384 (14.5) 489 (18.8) 527 (20) Non-Hispanic white 1200 (11.5) 221 (8.6) 302 (11.4) 332 (12.7) 345 (13.1) Non-Hispanic black 4578 (43.8) 1030 (40) 1131 (42.8) 1122 (43) 1295 (49.2) Other Hispanic 1989 (19.0) 771 (29.9) 569 (21.5) 412 (15.8) 237 (9) Other Race 1033 ( 9.9) 295 (11.5) 256 (9.7) 253 (9.7) 229 (8.7) Education, n (%) < 0.001 <9th Grade 1134 (10.8) 182 (7.1) 254 (9.6) 308 (11.8) 390 (14.8) 9th-11th Grade 1512 (14.5) 320 (12.4) 354 (13.4) 394 (15.1) 444 (16.9) High school graduate 2347 (22.4) 521 (20.2) 611 (23.1) 603 (23.1) 612 (23.2) Some college 2988 (28.6) 751 (29.2) 733 (27.7) 740 (28.4) 764 (29) ≥College graduate 2477 (23.7) 801 (31.1) 690 (26.1) 563 (21.6) 423 (16.1) Marital Status, n (%) < 0.001 married 6345 (60.7) 1469 (57) 1579 (59.8) 1643 (63) 1654 (62.8) divorced 4113 (39.3) 1106 (43) 1063 (40.2) 965 (37) 979 (37.2) PIR, Mean ± SD < 0.001 Low income（<1.3） 3093 (32.2) 674 (28.4) 728 (29.9) 762 (31.9) 929 (38.5) Medium（1.3-3.5） 3604 (37.5) 888 (37.4) 912 (37.5) 935 (39.2) 869 (36) High income（ ≥3.5） 2906 (30.3) 810 (34.1) 794 (32.6) 689 (28.9) 613 (25.4) Dietary Data Energy,kcal 1938.0 (1434.0, 2570.0) 2011.0 (1488.0, 2660.5) 1934.0 (1432.0, 2552.0) 1906.5 (1417.0, 2539.2) 1892.0 (1387.0, 2537.0) < 0.001 Protein ,gm 74.5 (52.8, 100.9) 77.1 (55.3, 106.7) 75.0 (52.8, 100.4) 72.6 (52.3, 98.7) 73.3 (51.0, 99.0) < 0.001 Carbohydrate, gm 232.6 (167.8, 312.8) 231.7 (166.5, 314.2) 231.3 (168.2, 311.4) 234.0 (169.2, 312.5) 232.8 (167.2, 313.0) 0.877 Total sugars,gm 95.3 (58.2, 143.7) 93.4 (55.7, 142.6) 95.6 (59.7, 140.3) 96.6 (59.6, 144.7) 95.4 (58.2, 147.5) 0.118 Dietary fiber,gm 14.6 (9.6, 21.6) 14.9 (9.9, 21.6) 14.7 (9.8, 21.5) 15.0 (9.7, 21.7) 14.1 (9.2, 21.4) 0.019 Total fat,gm 71.3 (47.4, 102.0) 74.8 (51.2, 108.0) 71.8 (47.4, 100.6) 70.8 (46.9, 101.1) 68.0 (44.6, 98.5) < 0.001 Examination Data BMI,kg/m2 28.9 ± 6.6 25.9 ± 5.8 28.3 ± 6.4 30.1 ± 6.5 31.3 ± 6.3 < 0.001 Wait circumference,cm 99.1 ± 16.0 90.5 ± 14.4 97.3 ± 15.3 102.4 ± 15.2 106.2 ± 14.6 < 0.001 SBP, mmHg 122.8 ± 17.7 120.1 ± 17.5 122.1 ± 17.7 123.2 ± 17.5 125.6 ± 17.7 < 0.001 DBP, mmHg 69.0 ± 12.0 67.8 ± 11.6 68.5 ± 11.8 69.1 ± 12.1 70.5 ± 12.4 < 0.001 Laboratory Data Hemoglobin A1c (%) 5.8 ± 1.1 5.5 ± 0.7 5.6 ± 0.9 5.8 ± 1.1 6.1 ± 1.4 < 0.001 FPG,mmol/L 6.1 ± 2.0 5.6 ± 1.2 5.8 ± 1.5 6.1 ± 2.0 6.7 ± 2.7 < 0.001 TG,mmol/L 1.2 (0.8, 1.7) 0.6 (0.5, 0.8) 1.0 (0.8, 1.1) 1.4 (1.2, 1.6) 2.2 (1.8, 2.9) < 0.001 LDL-cholesterol ,mmol/L 2.9 (2.3, 3.5) 2.6 (2.1, 3.2) 2.9 (2.3, 3.5) 3.0 (2.5, 3.6) 3.1 (2.4, 3.8) < 0.001 HDL-Cholesterol ,mmol/L 1.3 (1.1, 1.6) 1.7 (1.4, 2.0) 1.4 (1.2, 1.7) 1.2 (1.1, 1.4) 1.0 (0.9, 1.2) < 0.001 Questionnaire Data Hypertension, n (%) Yes 3738 (35.7) 680 (26.4) 891 (33.7) 978 (37.5) 1189 (45.2) < 0.001 Diabetes, n (%)Yes 1293 (12.4) 166 (6.4) 255 (9.7) 378 (14.5) 494 (18.8) < 0.001 CVD, n (%)Yes 419 ( 4.0) 81 (3.1) 90 (3.4) 103 (3.9) 145 (5.5) < 0.001 Secondary calculated data MAP, mmHg 90.5 ± 11.7 88.7 ± 11.6 89.9 ± 11.5 90.7 ± 11.7 92.5 ± 11.7 < 0.001 ePWV,m/s 7.7 (6.4, 9.8) 7.1 (6.2, 9.3) 7.6 (6.4, 9.7) 8.0 (6.6, 10.0) 8.1 (6.8, 9.9) < 0.001 Abbreviations: BMI, body mass index; PIR, family poverty income ratio; SBP,systolic blood pressure;DBP: diastolic blood pressure;, FBG: fasting blood glucose;TG,Triglyceride;CVD, Cardiovascular disease; MAP,mean arterial pressure;ePWV,Estimated pulse wave velocity;Quartile 1,VAI2.41.All statistical analyses in Table 1 were weighted using sample weights provided by NHANES to ensure that the results are nationally representative. Table2 Linear multiple regression analysis model to assess the relationship between visceral adiposity indexs and estimated pulse wave velocity concentrations. Variable Model 1 Model 2 Model 3 Model 4 Crude.Coefficient (95CI) P -value Adjusted.Coefficient (95CI) P -value Adjusted.Coefficient (95CI) P -value Adjusted.Coefficient (95CI) P -value VAI (0.2SD) 0.1 (0.04,0.17) 0.003 0.1 (0.03~0.17) 0.004 0.09 (0.02~0.17) 0.009 0.09 (0.02~0.16) 0.007 Quartile1 (<0.87) 0(Ref) 0(Ref) 0(Ref) 0(Ref) Quartile2 (0.87-1.42) 0.31 (0.19~0.43) <0.001 0.34 (0.22~0.46) <0.001 0.3 (0.19~0.42) <0.001 0.27 (0.16~0.38) <0.001 Quartile3 (1.42-2.41) 0.54 (0.42~0.65) <0.001 0.6 1(0.48~0.72) <0.001 0.53 (0.41~0.65) <0.001 0.49 (0.37~0.6) 2.41) 0.62 (0.5~0.74) <0.001 0.67 (0.56~0.79) <0.001 0.61(0.48~0.72) <0.001 0.53 (0.41~0.64) <0.001 Trend.test <0.001 <0.001 <0.001 <0.001 Abbreviations: CI,Confidence Interval;PIR,Poverty income ratio; Model 1: unadjusted; Model 2: adjusted for gender+race; Model 3: adjusted for Model 2+(educational level and PIR ); Model 4:adjusted for Model3+(Hypertension,Diabetes,Cardiovascular disease,Systolic blood pressure,Diastolic blood pressure,Smoking and Alcohol consumption) Additional Declarations No competing interests reported. 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10:04:27","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":252977,"visible":true,"origin":"","legend":"\u003cp\u003eRestricted cubic spline analysis of the relationship between VAI and ePWV.\u003c/p\u003e","description":"","filename":"Figure2300DPI.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5660240/v1/4499b721aac612f53f5e3dce.jpg"},{"id":79179204,"identity":"d71e11d6-c735-44f6-af5b-4d2fc12b203b","added_by":"auto","created_at":"2025-03-25 10:12:27","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":531552,"visible":true,"origin":"","legend":"\u003cp\u003eRestricted cubic spline analysis stratified by age, sex, etc.\u003c/p\u003e","description":"","filename":"Figure3300.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5660240/v1/5378dd766d362916738e5098.jpg"},{"id":79179199,"identity":"21bf541e-7d62-4ae0-8390-e7858845b291","added_by":"auto","created_at":"2025-03-25 10:12:27","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":1248202,"visible":true,"origin":"","legend":"\u003cp\u003eForest plot of subgroup analysis of the relationship between VAI and ePWV.\u003c/p\u003e","description":"","filename":"Figure4300DPI.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5660240/v1/a0b42de5a42d3a6c0934f005.jpg"},{"id":79180939,"identity":"a677e2fd-5369-4bde-940c-fcfbcb5c5e6a","added_by":"auto","created_at":"2025-03-25 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cardiovascular disease has increased significantly as a result of economic growth and an ageing population[\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. A loss of arterial elasticity with age is often observed. Progressive stiffening of the vessel wall is an inherent physiological feature associated with vascular aging[\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]. However, this feature is exacerbated by ageing and chronic non-communicable diseases. As an important marker of vascular ageing, arterial stiffness is important in the assessment of vascular health[\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e]. It is also associated with the development of cardiac, cerebrovascular and diabetic diseases. A growing number of studies have confirmed the potential positive association between the degree of atherosclerosis and these diseases[\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eStudies of arterial stiffness are currently a hot topic in the field. The current study has confirmed the diagnostic role of arterial stiffness in the assessment of cardiovascular disease risk [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. Carotid-femoral pulse wave velocity (cf-PWV) is considered the gold standard for assessing aortic stiffness [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. Despite the non-invasive nature of (cf-PWV) and brachial-ankle pulse wave velocity (baPWV) measurements. However, the clinical use of this method is currently extremely limited due to the need for specialised equipment [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. Estimated pulse wave velocity (ePWV) is a new index based on mean blood pressure (MBP) and age that has now been shown to be a reliable predictor of atherosclerosis.Although estimated PWV is not a complete replacement for cf-PWV, recent clinical studies have demonstrated its ability to independently predict cardiovascular disease events and survival [\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e, \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eObesity has been identified as a global epidemiologic challenge and its prevalence continues to rise despite several public health measures currently in place worldwide [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. Recent studies have shown that visceral adipose tissue (VAT) is a more reliable indicator of obesity than total body fat mass [\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e]. There is a potential link between visceral obesity and a variety of conditions, including vascular aging, carotid atherosclerosis, metabolic disorders, and increased all-cause mortality [\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e, \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e]. However, imaging techniques such as computed tomography (CT) and magnetic resonance imaging (MRI) are costly, time-consuming, and radiation intensive. There are still major obstacles to universal clinical application. VAI, calculated from waist circumference, body mass index (BMI), triglyceride levels, and high-density lipoprotein cholesterol (HDL-C), has been identified as a gender-specific index. Current studies have demonstrated that VAI is a reliable predictor of a variety of disease states, particularly associated with obesity [\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e, \u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e].VAI is a novel index for the assessment of obesity that integrates a variety of metabolic indicators. It can reflect the distribution of visceral fat and metabolic status more comprehensively. ePWV is an important indicator for assessing arterial stiffness. ePWV is closely related to metabolic health and the onset and progression of cardiovascular disease. However, there are relatively few studies on the relationship between VAI and ePWV. The purpose of this study was to analyze the potential relationship between VAI and ePWV using data from a national survey.\u003c/p\u003e"},{"header":"Methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003eStudy design and population in NHANES\u003c/h2\u003e \u003cp\u003eThis study is based on data from NHANES, a US national survey conducted from 2007 to 2016 using a complex stratified multistage probability sampling design to ensure national representation. The NHANES study protocol was approved by the Research Ethics Review Board of the National Center for Health Statistics, and written informed consent was obtained from all participants.The study report was prepared in accordance with the Enhanced Guidelines for Reporting Observational Studies in Epidemiology. An ethical exemption was granted by the Academic Review Board of the First People's Hospital of Yibin, as the raw data used in this study were obtained from publicly available databases.This study analyzed data from five NHANES survey cycles (2007\u0026ndash;2008, 2009\u0026ndash;2010, 2011\u0026ndash;2012, 2013\u0026ndash;2014 and 2015\u0026ndash;2016). And the final analysis data were obtained after strict inclusion and exclusion criteria. The final analysis included 10,458 individuals. (\u003cb\u003eThe flow chart is shown in\u003c/b\u003e Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003eAssessment of visceral adiposity index\u003c/h3\u003e\n\u003cp\u003eFor men and women, VAI was calculated using two separate equations: for men, VAI = (WC/[39.68\u0026thinsp;+\u0026thinsp;1.88 \u0026times; BMI)] \u0026times; (TG/1.03) \u0026times; (1.31/HDL), and for women VAI = [WC/(36.58\u0026thinsp;+\u0026thinsp;1.89 \u0026times; BMI)] \u0026times; (TG/0.81) \u0026times; (1.52/HDL), where BMI is body mass index (kg/m2), WC is waist circumference (cm), TG is triglycerides (mmol/L), and HDL is high-density lipoprotein in mmol/L[\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e].\u003c/p\u003e\n\u003ch3\u003eVAI normalization process\u003c/h3\u003e\n \u003cp\u003eIn the subsequent analyses of this study, we standardized the visceral adiposity index (VAI) to 0.2 standard deviations (SD) to improve the interpretability of the model and the stability of the results. Specifically, we calculated the mean and standard deviation of the VAI and divided each participant's VAI value minus the mean by 0.2 times the standard deviation. This standardization method is very common in epidemiological and biostatistical studies, especially when dealing with variables with different scales. With this treatment, the regression coefficient can be interpreted as the effect of each 0.2 standard deviation increase in VAI on estimated pulse wave velocity (ePWV).\u003c/p\u003e \n\u003ch3\u003eAssessment of ePWV\u003c/h3\u003e\n\u003cp\u003eThe Arterial Stiffness Reference Values Collaborative Group formulae, as described by Greve et al, were used to calculate ePWV using age (years) and mean blood pressure (MBP, mmHg),as follows: ePWV\u0026thinsp;=\u0026thinsp;9.587\u0026thinsp;\u0026minus;\u0026thinsp;0.402 \u0026times; age\u0026thinsp;+\u0026thinsp;4.560 \u0026times; 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e\u0026times; age\u003csup\u003e2\u003c/sup\u003e-2.621 \u0026times; 10\u003csup\u003e\u0026minus;\u0026thinsp;5\u003c/sup\u003e \u0026times; age\u003csup\u003e2\u003c/sup\u003e \u0026times;MBP\u0026thinsp;+\u0026thinsp;3.176 \u0026times; 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e \u0026times;age \u0026times;MBP-1.832 \u0026times;10\u003csup\u003e\u0026minus;\u0026thinsp;2\u003c/sup\u003e \u0026times; MBP. The formula for MBP is DBP plus 0.4 times the difference between SBP and DBP[\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e]. Blood pressure was recorded by a trained health technician, with participants having three consecutive measurements after 5 minutes of quiet sitting.\u003c/p\u003e\n\u003ch3\u003eCovariables Data Extraction\u003c/h3\u003e\n\u003cp\u003eThe selection of covariates was based on previous reports in the literature and clinical experience.The covariates included several demographic factors such as age (in years), sex (categorised as male and female), ethnicity, marital status, poverty income ratio (PIR) and education level.The laboratory results included triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL), glycosylated haemoglobin and fasting blood glucose. Dietary information was collected from two 24-hour recall interviews (midnight to midnight) in which participants reported the types and amounts of food and beverages (including all types of water) consumed in the 24 hours prior to the interview, along with an estimate of energy, protein, carbohydrate, fibre, total fat, and sugar intakes, and information on hypertension, diabetes, coronary heart disease, and stroke. Detailed descriptions of the study variables are available on the official CDC website: \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ewww.cdc.gov/nchs/nhanes/.Diagnostic\u003c/span\u003e\u003cspan address=\"http://www.cdc.gov/nchs/nhanes/.Diagnostic\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e criteria for diabetes mellitus included an official diagnosis by a physician, use of glucose-lowering medication or insulin, a glycated haemoglobin (HbA1c) level of 6.5% or higher, a fasting blood glucose level of 7.0 mmol/L or higher, or a blood glucose level of 11.1 mmol/L or higher on a random blood glucose test or 2-hour oral glucose tolerance test. Hypertension was characterised by an average systolic blood pressure of 140 mmHg or higher and an average diastolic blood pressure of 90 mmHg or higher, as diagnosed by a physician or treated with antihypertensive medication.\u003c/p\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003eWeights processing\u003c/h2\u003e \u003cp\u003e To ensure the national representativeness of the study results, we processed sample weights in strict accordance with official NHANES guidelines. As this study integrated NHANES data from five cycles from 2007 to 2016, we used the following weight processing steps:1. weight standardization: sample weights for each cycle were standardized using the formula: standardized weight\u0026thinsp;=\u0026thinsp;original weight/mean of weights for that cycle. 2. weight merging: the standardized weights were merged into a single unified weight using the formula: merged weight\u0026thinsp;=\u0026thinsp;standardized weight/number of cycles. 3. weighted analyses: all statistical analyses were performed using the merged weights to ensure that the results reflected the true picture of the US national population.\u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003eStatistical Analysis and sensitivity analysis\u003c/h3\u003e\n\u003cp\u003e The sample was weighted and stratified according to NHANES guidelines, and the NHANES study population was categorised into four different groups based on VAI quartiles. Categorical data were presented as frequencies (expressed as percentages), while continuous variables were presented using the mean and standard deviation if the distribution was normal, and the median and interquartile range (IQR) for skewed distributions. Comparative analyses were performed using one-way analysis of variance (ANOVA) for continuous variables with normal distributions, the Kruskal-Wallis H test for continuous variables with skewed distributions, and the chi-squared test for categorical variables to assess statistical significance between different groups.\u003c/p\u003e \u003cp\u003ePrior to linear regression analysis, we performed an initial test of the distribution of the dependent variable (estimated pulse wave velocity, ePWV). Although the original distribution of ePWV may not be exactly normal, the key assumption of the linear regression model is that the residuals should be approximately normal. Therefore, the distribution of the residuals was tested as follows: histograms of the residuals were plotted and the Shapiro-Wilk normality test was performed. The results show that the distribution of the residuals is close to normal and the p-value of the Shapiro-Wilk test is greater than 0.05, indicating that there is not enough evidence to reject the hypothesis that the residuals are normally distributed. We also attempted to log-transform the ePWV to improve the normality of its distribution, but ultimately decided to use the original ePWV values for analysis because the interpretation of the log-transformed values was not as intuitive as the original values. We confirmed the appropriateness of using the raw ePWV values for linear regression analysis using residual tests.\u003c/p\u003e\u003cp\u003eIn this investigation, potential confounding variables were first identified through a screening process, and then a series of statistical analyses were performed to explore the relationship between VAI and ePWV.In this study, the restricted cubic spline method was used to model the exposure-outcome relationship. Where potential non-linear correlations were detected, inflection point analyses were performed to identify inflection points where the relationship between VAI and ePWV changed significantly.Univariate and multivariate linear regression analyses and model analyses with stepwise adjustment for covariates were then performed to further explore the potential relationship between VAI and ePWV. For modelling analyses, we compared VAI when it was used as a continuous or categorical variable. To assess the relationship between VAI and ePWV, we used weighted multivariate linear regression analyses with stepwise adjustment for multiple potential confounders. We developed four different models with progressively more adjusted variables. Model 1 did not adjust for any confounders and assessed only the crude correlation between VAI and ePWV; model 2 adjusted for sex and race; model 3 further adjusted for education level and PIR based on model 2; and model 4 further adjusted for other confounders such as hypertension, diabetes mellitus, cardiovascular disease, SBP, DBP, cigarette smoking and alcohol consumption based on model 3. In addition, we examined the interactions between the main confounders and VAI and performed several sensitivity analyses to ensure the robustness of the results. We also performed detailed diagnostic analyses of the final model, including residual analysis, linear hypothesis testing, and multiple covariance testing, to ensure the applicability of the model and the reliability of the results.\u003c/p\u003e \u003cp\u003eFor the statistical computations, the Free Statistics software version 2.0 (Beijing, China) and the R programming environment version 4.3.2 were utilized. The p-values reported are two-tailed, with a threshold of less than 0.05 set to determine statistical significance, thereby allowing for the identification of associations that are unlikely to occur by chance.\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003e\u003cstrong\u003eStudy population\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study analyzed data from five NHANES survey cycles (2007-2008, 2009-2010, 2011-2012, 2013-2014, and 2015-2016), initially excluding participants younger than 20 years (n=21,387) from the total of 50,588 individuals. We then excluded participants with missing systolic blood pressure data (n=4129), diastolic blood pressure data (n=63), waist circumference data (n=1142), body mass index data (n=55), triglyceride data (n=12911), and other variables (n=443).With strict exclusion, the missing covariate data for the remaining participants was less than 5%. As a result, multiple interpolation was used to cleanse the study, resulting in the inclusion of 10458 subjects in the final analysis(\u003cstrong\u003eFigure 1\u003c/strong\u003e).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCharacteristics of the participants\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll participants were divided into four groups according to baseline VAI quartiles. As shown in Table 1, group Q1 (VAI: \u0026lt;0.87, N=2575); group Q2 (VAI: 0.87-1.42, N=2642); group Q3 (VAI: 1.42-2.41, N=2608); and group Q4 (VAI: \u0026gt;2.41, N=2633). Data on demographic characteristics, dietary intake, examination data, laboratory data, and questionnaires for all participants in each subgroup are shown in\u003cstrong\u003e\u0026nbsp;Table 1\u003c/strong\u003e.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eUnivariate and Multivariate Linear Regression Analyses\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn this study, univariate regression analysis between VAI and various covariates and ePWV was systematically analysed using linear regression analysis. The results indicated a possible positive association between VAI and ePWV (\u0026beta;: 0.1; [95% CI: 0.04,0.17], \u003cem\u003eP\u003c/em\u003e=0.003). In addition, univariate analyses performed with all covariates for exposure suggested statistically significant differences between age, male, race, education, PIR and energy intake and outcomes (all \u003cem\u003eP\u003c/em\u003e\u0026lt;0.001). Detailed results are presented in \u003cstrong\u003eSupplementary Table 1\u003c/strong\u003e.\u003c/p\u003e\n\u003cp\u003eCovariance tests for covariates were then performed using methods such as variance inflation factor (VIF). The results indicated a significant colinearity between energy intake, carbohydrate intake, total sugar intake, total fat intake, BMI, WC, TC, TG, HDL-c and LDL-c. Detailed results are presented in \u003cstrong\u003eSupplementary Table 2\u003c/strong\u003e. Covariates that were significantly colinearity were removed in subsequent multivariate analyses.\u003c/p\u003e\n\u003cp\u003eIn the multivariate linear regression analysis, we found a potential positive association between VAI and ePWV. This association remained significant after adjustment for multiple confounders.In model 1, no adjustment for covariates was made and the results indicated a possible positive correlation between VAI and ePWV (\u0026beta;: 0.1; [95% CI: 0.04,0.17], \u003cem\u003eP\u003c/em\u003e=0.003). Stable positive associations were also observed in models with stepwise adjustment for covariates. In model 4, adjusted for all covariates, the results were suggestive (\u0026beta;: 0.09; [95% CI: 0.02,0.16], \u003cem\u003eP\u003c/em\u003e=0.007). All participants were then divided into four different groups according to the quartiles of the VAI. The lowest group (Q1, VAI \u0026lt; 0.87) was used as the reference group. Linear multivariate regression analysis continued with four different models. No covariate adjustment was performed in model 1. Groups Q2 (\u0026beta;: 0.31; [95% CI: 0.19,0.43], \u003cem\u003eP\u003c/em\u003e\u0026lt;0.001), Q3 (\u0026beta;: 0.54; [95% CI: 0.42,0.65], \u003cem\u003eP\u003c/em\u003e\u0026lt;0.001) and Q4 (\u0026beta;: 0.62; [95% CI: 0.50,0.74], \u003cem\u003eP\u003c/em\u003e\u0026lt;0.001) showed a progressively higher positive correlation. The results remained stable and consistent in model 4 adjusted for all covariates. The results remained stable and consistent in models with stepwise adjustment for covariates. In model 4 adjusted for all covariates, the results were Q2 (\u0026beta;: 0.27; [95% CI: 0.16,0.38], \u003cem\u003eP\u003c/em\u003e\u0026lt;0.001), Q3 (\u0026beta;: 0.49; [95% CI: 0.37,0.60], \u003cem\u003eP\u003c/em\u003e\u0026lt;0.001) and Q4 (\u0026beta;: 0.53; [95% CI: 0.41,0.64], \u003cem\u003eP\u003c/em\u003e\u0026lt;0.001). Detailed results are presented in \u003cstrong\u003eTable 2\u003c/strong\u003e. We also tested for interactions between the main confounders and VAI and found no significant interactions. The results of the sensitivity analysis were consistent with those of the main analysis, further validating the robustness of the results. Model diagnostic analysis showed that the residual distribution was close to normal, the linearity assumption was valid, and no significant multicollinearity problems were found.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eRestricted cubic spline models\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eRestricted cubic spline models were constructed to fit the curve between VAI and ePWV. Smoothed curve fits between variables are indicated by the solid red line, and shaded bands indicate 95% confidence intervals. Adjustment for covariates revealed a non-linear relationship between VAI values and ePWV (p for non-linearity \u0026lt;0.001)\u003cstrong\u003e(Figure 2)\u003c/strong\u003e.\u003c/p\u003e\n\u003cp\u003eInflection point analysis was then performed and the results suggested a significant positive correlation between VAI and ePWV when VAI was less than 1.48 (1.38-1.57) (\u0026beta;:0.49; [95% CI: 0.31,0.67], \u003cem\u003eP\u003c/em\u003e\u0026lt;0.001). However, there was no statistically significant correlation between the two when VAI exceeded 1.48 (\u0026beta;:0.06; [95% CI: -0.01,0.12], p= 0.09)( \u003cstrong\u003eSupplementary Table3 and Supplementary Figure1\u0026nbsp;\u003c/strong\u003e).\u003c/p\u003e\n\u003cp\u003eTo further validate the stability of the findings, we performed RCS analysis after stratifying the population. An \u0026quot;inverted L-shaped\u0026quot; non-linear relationship, consistent with the whole population, was observed in the male, female and age \u0026lt;60 years strata. The age \u0026gt;60 years, hypertension and diabetes strata showed higher levels of VAI and ePWV than the total population (\u003cstrong\u003eSupplementary Table 4\u003c/strong\u003e). Potential negative linear associations were found in stratified RCS analyses(\u003cstrong\u003eFigure 3\u003c/strong\u003e).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSubgroup analysis and Sensitivity analysis\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTo further validate the potential relationship between VAI and ePWV, we also analysed participants in subgroups based on age, sex, race, education, history of hypertension, history of diabetes, and history of CVD (\u003cstrong\u003eFigure 4\u003c/strong\u003e). When VAI was subgrouped as a continuous and categorical variable, the results were consistent with the overall results. Significant interactions were found in the subgroups of age \u0026gt;60 years, hypertension, and diabetes (\u003cstrong\u003eSupplementary Table 5 and\u0026nbsp;Supplementary Table 6\u003c/strong\u003e).\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eIn this cross-sectional study, we examined the relationship between the VAI, a novel indicator of adiposity distribution and dysfunction, and ePWV, a marker of arterial stiffness, in a representative sample of US adults. Our results showed a significant positive association between VAI and ePWV, particularly below a VAI inflection point of 1.48. This association remained robust after adjustment for various confounders and was consistent across subgroup analyses, except for potential interactions within the age\u0026thinsp;\u0026gt;\u0026thinsp;60 years, hypertension, and diabetes subgroups. These findings suggest that abdominal obesity, as quantified by VAI, may be an important contributor to atherosclerosis. This study provides valuable insights into the complex interplay between obesity and vascular health and highlights the need for further research to develop targeted interventions to mitigate the impact of abdominal obesity on arterial stiffness and reduce cardiovascular risk.\u003c/p\u003e \u003cp\u003eArterial stiffness, which progresses with age, results in thickening of the arterial walls and narrowing of the lumen [\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e]. Arterial stiffness is largely determined by the severity of ischaemia in the affected organ, with most patients showing few clinical signs in the early stages.Research has shown that arterial stiffness is associated with an increased risk of cardiovascular disease, stroke and other diseases, yet a sensitive and specific laboratory diagnostic method for early arterial stiffness is lacking[\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. As cf-PWV has not been widely used in clinical practice, ePWV has played a role in raising awareness of the risks of aortic stiffness and assisting physicians in its clinical application[\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e].The study involved using a previously published formula to assess ePWV and investigate its correlation with visceral adiposity index.Some recent research suggests a positive association between body fat levels and arterial stiffness, while other studies suggest a negative or no association [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e].A study from the United States, which used VFA to accurately measure visceral fat to assess obesity, showed that the normal weight visceral obese group was associated with arterial stiffness and increased 10-year cardiovascular risk [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e].A study from Spain, which also used body mass index, ratio and BMI to assess obesity, showed that indicators of obesity were inversely correlated with arterial stiffness, as measured by the cardio-ankle vascular index (CAVI) and brachial-ankle pulse wave velocity[\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eA study from Japan showed that measures of obesity, such as fat volume index, body mass index and body circumference index, were positively correlated with brachial-ankle PWV [\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e]. In a study of Korean adults, ePWV was found to correlate more significantly with waist circumference than with BMI, supporting the concept that visceral obesity is more closely associated with arterial stiffness than total obesity [\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e].The results of our study regarding the association between visceral adiposity index and vascular stiffness are consistent with this. Therefore, our study offers the following advantages: The first notable advantage is that ePWV, as a marker of arterial stiffness, is more accessible and easily reproducible in clinical practice; the second important feature is that VAI, as a measure of visceral adipose function, is a sex-specific index that includes simple anthropometric and common epidemiological parameters, and this calculated value provides an association between abdominal obesity and arterial stiffness; Thirdly, the study we conducted this time had a sufficiently large sample size; and fourthly, for clinical purposes, this association provides a simple, valid and comprehensive assessment of cardiovascular disease in primary prevention populations and even in Stage 0 prevention groups, which is crucial for reducing the global burden of cardiovascular events. Future clinical guidelines and public health policies on obesity and cardiovascular health may be influenced by the results of this study.\u003c/p\u003e \u003cp\u003eA number of underlying mechanisms have been proposed to elucidate the initiation and progression of obesity and arterial stiffness.On the one hand, visceral adiposity actively secretes large amounts of inflammatory mediators, which can lead to increased insulin resistance, enhanced oxidative stress and endothelial cell dysfunction, all of which induce structural changes in the arterial wall and thus promote arterial stiffness[\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e]. A critical element to consider is the role of adipokines, bioactive peptides with endocrine activity released from visceral adipose tissue. For example, leptin, an adipokine, is thought to activate the sympathetic nervous system. This activation can lead to increased blood pressure and increased arterial stiffness[\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e]. On the other hand, levels of lipocalin, which has anti-inflammatory and anti-atherosclerotic properties, tend to be reduced in individuals with visceral obesity [\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e].It is also noteworthy that visceral adipose tissue actively expresses and releases components of the renin-angiotensin system, such as angiotensinogen and angiotensin II. The involvement of this system in the development of hypertension and vascular remodelling is well established, and both play an important role in atherosclerosis [\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e].In addition, abdominal obesity and atherosclerosis share common risk factors, including hypertension, diabetes, dyslipidaemia and sedentary lifestyle.It is conceivable that these diseases, while increasing atherosclerosis, may also have feedback mechanisms that indirectly contribute to abdominal obesity [\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e, \u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eIn the present study, we found a significant positive correlation between VAI and ePWV. However, the basal level of ePWV was significantly higher than the total population level in the subgroups of patients older than 60 years, hypertensive patients and diabetic patients(\u003cb\u003eSupplementary Table\u0026nbsp;7\u003c/b\u003e). An interaction between VAI and ePWV was observed in these subgroups. This phenomenon suggests that obesity seems to have an \"inverse effect\" on atherosclerosis in these specific subgroups. The higher the degree of VAI, the less pronounced the increase in ePWV compared to the total population. This phenomenon may be related to the interaction of several pathophysiological mechanisms associated with VAI .\u003c/p\u003e \u003cp\u003eThe relationship between visceral obesity and arterial stiffness is not a simple linear one. Visceral obesity has been shown to be strongly associated with increased arterial stiffness.VAT secretes a variety of inflammatory factors and adipokines, such as leptin and lipocalin. These factors contribute to increased arterial stiffness by affecting insulin resistance, oxidative stress and endothelial cell function, promoting structural changes in the arterial wall [\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e] However, in specific populations, visceral obesity may have a complex effect on arterial stiffness through other mechanisms.In the subgroup aged \u0026amp;amp;amp;gt;60 years, ePWV levels were significantly higher than in the total population. This is consistent with previous studies showing that atherosclerosis increases with age [\u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e]. However, the positive correlation between VAI and ePWV was attenuated and even reversed in this subgroup. This phenomenon may be related to the pathophysiological mechanisms of atherosclerosis in the elderly population. With age, the ratio of collagen to elastin in the arterial wall changes, leading to increased arterial stiffness [\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e]. In this context, obesity may have a \"buffering\" effect on arterial stiffness by affecting the metabolic and inflammatory state of the vascular wall. In addition, there is a high prevalence of comorbid chronic diseases (e.g. hypertension, hyperuricemia,diabetes mellitus) in the elderly population, and these diseases may themselves exacerbate arterial stiffness, thus masking the direct effect of obesity on arterial stiffness [\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e, \u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e].In the hypertensive subgroup, ePWV values were also significantly higher than in the total population. Hypertension is a major risk factor for increased atherosclerosis. It can lead to structural remodelling of the arterial wall and increased stiffness by increasing blood flow shear and vessel wall pressure [\u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e]. In this subgroup, the interaction between VAI and ePWV may be related to the effect of obesity on blood pressure regulation. Obese patients tend to have hyperactivation of the sympathetic nervous system, which may further exacerbate elevated blood pressure and increased arterial stiffness in hypertensive patients [\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e37\u003c/span\u003e]. However, obesity may also have a complex effect on arterial stiffness in hypertensive patients by affecting the renin-angiotensin-aldosterone system (RAAS) and vascular endothelial function. On the one hand, obesity may exacerbate arterial stiffness by increasing RAAS activation; on the other hand, obesity may have a protective effect on arterial stiffness by affecting vascular endothelial function [\u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e38\u003c/span\u003e].In the diabetic subgroup, ePWV values were significantly higher than in the total population, which is consistent with previous studies indicating the presence of higher arterial stiffness in diabetic patients [\u003cspan citationid=\"CR39\" class=\"CitationRef\"\u003e39\u003c/span\u003e]. Patients with diabetes tend to have insulin resistance, hyperglycaemia and a chronic inflammatory state. Together, these factors lead to increased glycosylation and oxidative stress in the arterial wall, exacerbating atherosclerosis [\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e]. In this subgroup, the interaction between VAI and ePWV may be related to the effect of obesity on the metabolic status of diabetic patients. Obese patients tend to have more severe insulin resistance, which may further exacerbate elevated blood glucose and increased arterial stiffness in diabetic patients [\u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e41\u003c/span\u003e]. However, obesity may also have some protective effect on atherosclerosis by affecting lipid metabolism and inflammatory status in diabetic patients. For example, obese patients tend to have higher levels of high-density lipoprotein cholesterol (HDL-C), which may have a protective effect on oxidative stress and the inflammatory state of the arterial wall .\u003c/p\u003e \u003cdiv id=\"Sec17\" class=\"Section2\"\u003e \u003ch2\u003eLimitations\u003c/h2\u003e \u003cp\u003eWe acknowledge several limitations of our study. First, in the cross-sectional analyses, we excluded data with missing exposures and outcomes, which may introduce selection bias.Second, some of the data were collected by questionnaire, which may have introduced significant recall bias. Despite accounting for various confounding factors, there may still be other yet-to-be-discovered factors that could influence the results of the study and thus affect the validity of the findings.Further rigorously designed clinical trials are needed to investigate the potential association between visceral adiposity index and ePWV. In addition, the cross-sectional design of our study did not allow us to establish a causal relationship between VAI and ePWV.\u003c/p\u003e \u003c/div\u003e"},{"header":"Conclusions","content":"\u003cp\u003eIn this cross-sectional study, a significant positive association was observed between visceral adiposity index and estimated pulse wave velocity in a representative sample of US adults, particularly below a VAI inflection point of 1.48. This association remained robust after adjustment for various confounders and was consistent across subgroup analyses, highlighting the potential role of abdominal obesity in atherosclerosis and the importance of addressing this issue to reduce cardiovascular risk. Further research is warranted to explore interventions that may mitigate the effects of abdominal obesity on atherosclerosis.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAuthor Contributors\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors of this work have satisfied the ICMJE criteria for authorship and have significantly contributed to the manuscript through tasks such as data analysis and interpretation, drafting, critical revision, and final approval. The study was designed by S.Y. and Y.L., while Y.L. authored the manuscript. S.Y. collected all clinical data. S.Y. and J.W. statistically analyzed clinical data. The report was thoroughly reviewed and edited by all authors, who subsequently granted their approval for the final draft.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study was supported by Medical Research Program of Yibin Municipal Health Commission (2024YW001).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgement\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe thank all the researchers and participants involved in NHANES databases. We thanks Dr. Jie Liu (People\u0026rsquo;s Liberation Army of China General Hospital, Beijing, China) for helping with research design. We thank the Science and Technology Programme of Yibin Municipal Health Commission for supporting this study.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eData availability statement\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll the raw data required in this study can be extracted from the NHANES online website. For further inquiries, please contact the first author or corresponding author.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eClinical trial number:\u0026nbsp;\u003c/strong\u003eNot applicable\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare no competing interests.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics statement\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe current study used publicly available summary data, and ethical review and approval for research involving human participants have been conducted in accordance with local legislative and institutional requirements for preliminary studies in which data were collected. Therefore, written informed consent was not required for this study, and no additional ethical approval was needed.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eORCID\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eYanwei Liu \u0026nbsp;https://orcid.org/0009-0006-1096-2862\u003c/p\u003e\n\u003cp\u003eShiqiang Yang \u0026nbsp;https://orcid.org/0000-0003-3138-1777\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eWang H, Yu X, Guo J, et al. Burden of cardiovascular disease among the Western Pacific region and its association with human resources for health, 1990-2021: a systematic analysis of the Global Burden of Disease Study 2021. Lancet Reg Health West Pac. 2024;51:101195. Published 2024 Sep 3. doi:10.1016/j.lanwpc.2024.101195\u003c/li\u003e\n\u003cli\u003eLee J, Song RJ, Musa Yola I, et al. Association of Estimated Cardiorespiratory Fitness in Midlife With Cardiometabolic Outcomes and Mortality. JAMA Netw Open. 2021;4(10):e2131284. Published 2021 Oct 1. doi:10.1001/jamanetworkopen.2021.31284\u003c/li\u003e\n\u003cli\u003eMengi S, Januzzi JL Jr, Cavalcante JL, et al. Aortic Stenosis, Heart Failure, and Aortic Valve Replacement. JAMA Cardiol. Published online October 16, 2024. doi:10.1001/jamacardio.2024.3486 \u003c/li\u003e\n\u003cli\u003eDangardt F, Charakida M, Georgiopoulos G, et al. Association between fat mass through adolescence and arterial stiffness: a population-based study from The Avon Longitudinal Study of Parents and Children. Lancet Child Adolesc Health. 2019;3(7):474-481. doi:10.1016/S2352-4642(19)30105-1 \u003c/li\u003e\n\u003cli\u003eFan Y, Yang S, Ruan L, Zhang C, Cao M. Association between Life\u0026apos;s Essential 8 and estimated pulse wave velocity among adults in the US: a cross-sectional study of NHANES 2011-2018. Front Public Health. 2024;12:1388424. Published 2024 May 30. doi:10.3389/fpubh.2024.1388424 \u003c/li\u003e\n\u003cli\u003eCovic A, Siriopol D. 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Estimated Pulse Wave Velocity Calculated from Age and Mean Arterial Blood Pressure. Pulse (Basel). 2017;4(4):175-179. doi:10.1159/000453073 \u003c/li\u003e\n\u003cli\u003ed\u0026apos;El-Rei J, Cunha MR, Mattos SS, et al. Microvascular Reactivity in Hypertensive Patients with High Body Adiposity. Estudo da Reatividade Microvascular em Pacientes Hipertensos com Adiposidade Corporal Elevada. Arq Bras Cardiol. 2020;115(5):896-904. doi:10.36660/abc.20190364 \u003c/li\u003e\n\u003cli\u003eAtaee Z, Aghaee A, Sobhani SR, et al. Evaluation of Arterial Stiffness and Its Relation to Innovative Anthropometric Indices in Persian Adults. Int J Hypertens. 2023;2023:2180923. Published 2023 Jan 23. doi:10.1155/2023/2180923 \u003c/li\u003e\n\u003cli\u003eVishram-Nielsen JKK, Laurent S, Nilsson PM, et al. Does Estimated Pulse Wave Velocity Add Prognostic Information?: MORGAM Prospective Cohort Project. Hypertension. 2020;75(6):1420-1428. doi:10.1161/HYPERTENSIONAHA.119.14088 \u003c/li\u003e\n\u003cli\u003eGong J, Han Y, Gao G, et al. Sex-specific difference in the relationship between body fat percentage and arterial stiffness: Results from Fuzhou study. J Clin Hypertens (Greenwich). 2023;25(3):286-294. doi:10.1111/jch.14649 \u003c/li\u003e\n\u003cli\u003eZheng L, Sun A, Han S, et al. Association between visceral obesity and 10-year risk of first atherosclerotic cardiovascular diseases events among American adults: National Health and Nutrition Examination Survey. Front Cardiovasc Med. 2023;10:1249401. Published 2023 Aug 21. doi:10.3389/fcvm.2023.1249401 \u003c/li\u003e\n\u003cli\u003eSaz-Lara A, Cavero-Redondo I, Moreno-Herr\u0026aacute;iz N, et al. Association between body shape index and arterial stiffness: results of the EVasCu study and a meta-analysis. Int J Obes (Lond). Published online October 28, 2024. doi:10.1038/s41366-024-01663-8 \u003c/li\u003e\n\u003cli\u003eYoshida Y, Nakanishi K, Jin Z, et al. Association Between Progression of Arterial Stiffness and Left Ventricular Remodeling in a Community-Based Cohort. JACC Adv. 2023;2(5):100409. Published 2023 Jul 5. doi:10.1016/j.jacadv.2023.100409 \u003c/li\u003e\n\u003cli\u003eKim HL, Joh HS, Lim WH, et al. Associations of Estimated Pulse Wave Velocity with Body Mass Index and Waist Circumference among General Korean Adults. Metabolites. 2023;13(10):1082. Published 2023 Oct 15. doi:10.3390/metabo13101082 \u003c/li\u003e\n\u003cli\u003eArdissino M, McCracken C, Bard A, et al. Pericardial adiposity is independently linked to adverse cardiovascular phenotypes: a CMR study of 42 598 UK Biobank participants. Eur Heart J Cardiovasc Imaging. 2022;23(11):1471-1481. doi:10.1093/ehjci/jeac101 \u003c/li\u003e\n\u003cli\u003eEngin A. Endothelial Dysfunction in Obesity and Therapeutic Targets. Adv Exp Med Biol. 2024;1460:489-538. doi:10.1007/978-3-031-63657-8_17 \u003c/li\u003e\n\u003cli\u003eOpdebeeck B, Huysmans I, Van den Branden A, Orriss IR, D\u0026apos;Haese PC, Verhulst A. Deletion of the P2Y2 receptor aggravates internal elastic lamina calcification in chronic kidney disease mice through upregulation of alkaline phosphatase and lipocalin-2 [published correction appears in FASEB J. 2023 Aug;37(8):e23069. doi: 10.1096/fsb2.23069]. FASEB J. 2023;37(1):e22701. doi:10.1096/fj.202201044R\u003c/li\u003e\n\u003cli\u003eTakeda Y, Demura M, Yoneda T, Takeda Y. DNA Methylation of the Angiotensinogen Gene, AGT, and the Aldosterone Synthase Gene, CYP11B2 in Cardiovascular Diseases. Int J Mol Sci. 2021;22(9):4587. Published 2021 Apr 27. doi:10.3390/ijms22094587 \u003c/li\u003e\n\u003cli\u003eZheng J, Hu Y, Xu H, et al. Normal-weight visceral obesity promotes a higher 10-year atherosclerotic cardiovascular disease risk in patients with type 2 diabetes mellitus-a multicenter study in China. Cardiovasc Diabetol. 2023;22(1):137. Published 2023 Jun 12. doi:10.1186/s12933-023-01876-7\u003c/li\u003e\n\u003cli\u003eWest HW, Siddique M, Williams MC, et al. Deep-Learning for Epicardial Adipose Tissue Assessment With Computed Tomography: Implications for Cardiovascular Risk Prediction. JACC Cardiovasc Imaging. 2023;16(6):800-816. doi:10.1016/j.jcmg.2022.11.018 \u003c/li\u003e\n\u003cli\u003eEngin A. Endothelial Dysfunction in Obesity and Therapeutic Targets. Adv Exp Med Biol. 2024;1460:489-538. doi: 10.1007/978-3-031-63657-8_17. \u003c/li\u003e\n\u003cli\u003eVishram-Nielsen JKK, Laurent S, Nilsson PM, et al. Does Estimated Pulse Wave Velocity Add Prognostic Information?: MORGAM Prospective Cohort Project. Hypertension. 2020;75(6):1420-1428. doi:10.1161/HYPERTENSIONAHA.119.14088 \u003c/li\u003e\n\u003cli\u003eMarshall AG, Neikirk K, Afolabi J, et al. Update on the Use of Pulse Wave Velocity to Measure Age-Related Vascular Changes. Curr Hypertens Rep. 2024;26(3):131-140. doi:10.1007/s11906-023-01285-x \u003c/li\u003e\n\u003cli\u003eArdissino M, McCracken C, Bard A, et al. Pericardial adiposity is independently linked to adverse cardiovascular phenotypes: a CMR study of 42 598 UK Biobank participants. Eur Heart J Cardiovasc Imaging. 2022;23(11):1471-1481. doi:10.1093/ehjci/jeac101 \u003c/li\u003e\n\u003cli\u003eCassano V, Crescibene D, Hribal ML, et al. Uric Acid and Vascular Damage in Essential Hypertension: Role of Insulin Resistance. Nutrients. 2020;12(9):2509. Published 2020 Aug 19. doi:10.3390/nu12092509 \u003c/li\u003e\n\u003cli\u003ed\u0026apos;El-Rei J, Cunha MR, Mattos SS, et al. Microvascular Reactivity in Hypertensive Patients with High Body Adiposity. Estudo da Reatividade Microvascular em Pacientes Hipertensos com Adiposidade Corporal Elevada. Arq Bras Cardiol. 2020;115(5):896-904. doi:10.36660/abc.20190364\u003c/li\u003e\n\u003cli\u003eGong J, Han Y, Gao G, et al. Sex-specific difference in the relationship between body fat percentage and arterial stiffness: Results from Fuzhou study. J Clin Hypertens (Greenwich). 2023;25(3):286-294. doi:10.1111/jch.14649 \u003c/li\u003e\n\u003cli\u003eOpdebeeck B, Huysmans I, Van den Branden A, Orriss IR, D\u0026apos;Haese PC, Verhulst A. Deletion of the P2Y2 receptor aggravates internal elastic lamina calcification in chronic kidney disease mice through upregulation of alkaline phosphatase and lipocalin-2 [published correction appears in FASEB J. 2023 Aug;37(8):e23069. doi: 10.1096/fsb2.23069.]. FASEB J. 2023;37(1):e22701. doi:10.1096/fj.202201044R \u003c/li\u003e\n\u003cli\u003eLiu C, Pan H, Kong F, et al. Association of arterial stiffness with all-cause and cause-specific mortality in the diabetic population: A national cohort study. Front Endocrinol (Lausanne). 2023;14:1145914. Published 2023 Mar 8. doi:10.3389/fendo.2023.1145914 \u003c/li\u003e\n\u003cli\u003eZheng J, Hu Y, Xu H, et al. Normal-weight visceral obesity promotes a higher 10-year atherosclerotic cardiovascular disease risk in patients with type 2 diabetes mellitus-a multicenter study in China. Cardiovasc Diabetol. 2023;22(1):137. Published 2023 Jun 12. doi:10.1186/s12933-023-01876-7 \u003c/li\u003e\n\u003cli\u003eZou X, Zhou X, Li Y, et al. Gender-specific data-driven adiposity subtypes using deep-learning-based abdominal CT segmentation. Obesity (Silver Spring). 2023;31(6):1600-1609. doi:10.1002/oby.23741\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003e\u003cstrong\u003eTable 1\u003c/strong\u003e\u0026nbsp; Baseline characteristics of participants in NHANES (N = 10458).\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eVariables\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eTotal (n = 10458)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003cstrong\u003eQuartile1 (n = 2575)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eQuartile2 (n = 2642)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eQuartile3 (n = 2608)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eQuartile4 (n = 2633)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003ep\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eDemographics Data\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eGender, n (%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; Male\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5192 (49.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1368 (53.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1292 (48.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1254 (48.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1278 (48.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; Female\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5266 (50.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1207 (46.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1350 (51.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1354 (51.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1355 (51.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eAge,year\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e49.2 \u0026plusmn; 17.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e46.1 \u0026plusmn; 17.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e48.7 \u0026plusmn; 17.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e50.8 \u0026plusmn; 17.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e51.3 \u0026plusmn; 16.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eRace/Ethnicity, n (%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;Mexican American\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1658 (15.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e258 (10)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e384 (14.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e489 (18.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e527 (20)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp;\u0026nbsp;Non-Hispanic white\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1200 (11.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e221 (8.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e302 (11.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e332 (12.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e345 (13.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp;\u0026nbsp;Non-Hispanic black\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e4578 (43.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1030 (40)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1131 (42.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1122 (43)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1295 (49.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eOther Hispanic\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1989 (19.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e771 (29.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e569 (21.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e412 (15.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e237 (9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eOther Race\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1033 ( 9.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e295 (11.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e256 (9.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e253 (9.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e229 (8.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eEducation, n (%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026lt;9th Grade\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1134 (10.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e182 (7.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e254 (9.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e308 (11.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e390 (14.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;9th-11th Grade\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1512 (14.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e320 (12.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e354 (13.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e394 (15.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e444 (16.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; High school graduate\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2347 (22.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e521 (20.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e611 (23.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e603 (23.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e612 (23.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eSome college\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2988 (28.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e751 (29.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e733 (27.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e740 (28.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e764 (29)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026ge;College graduate\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2477 (23.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e801 (31.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e690 (26.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e563 (21.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e423 (16.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eMarital Status, n (%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003emarried\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6345 (60.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1469 (57)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1579 (59.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1643 (63)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1654 (62.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003edivorced\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e4113 (39.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1106 (43)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1063 (40.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e965 (37)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e979 (37.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003ePIR, Mean \u0026plusmn; SD\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; Low income（\u0026lt;1.3）\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e3093 (32.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e674 (28.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e728 (29.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e762 (31.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e929 (38.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Medium（1.3-3.5）\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e3604 (37.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e888 (37.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e912 (37.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e935 (39.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e869 (36)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; High income（\u0026nbsp;\u0026ge;3.5）\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2906 (30.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e810 (34.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e794 (32.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e689 (28.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e613 (25.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eDietary Data\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eEnergy,kcal\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1938.0 (1434.0, 2570.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2011.0 (1488.0, 2660.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1934.0 (1432.0, 2552.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1906.5 (1417.0, 2539.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1892.0 (1387.0, 2537.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eProtein ,gm\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e74.5 (52.8, 100.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e77.1 (55.3, 106.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e75.0 (52.8, 100.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e72.6 (52.3, 98.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e73.3 (51.0, 99.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eCarbohydrate, gm\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e232.6 (167.8, 312.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e231.7 (166.5, 314.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e231.3 (168.2, 311.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e234.0 (169.2, 312.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e232.8 (167.2, 313.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e0.877\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eTotal sugars,gm\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e95.3 (58.2, 143.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e93.4 (55.7, 142.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e95.6 (59.7, 140.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e96.6 (59.6, 144.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e95.4 (58.2, 147.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e0.118\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eDietary fiber,gm\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e14.6 (9.6, 21.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e14.9 (9.9, 21.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e14.7 (9.8, 21.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e15.0 (9.7, 21.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e14.1 (9.2, 21.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e0.019\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eTotal fat,gm\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e71.3 (47.4, 102.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e74.8 (51.2, 108.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e71.8 (47.4, 100.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e70.8 (46.9, 101.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e68.0 (44.6, 98.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eExamination Data\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eBMI,kg/m2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e28.9 \u0026plusmn; 6.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e25.9 \u0026plusmn; 5.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e28.3 \u0026plusmn; 6.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e30.1 \u0026plusmn; 6.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e31.3 \u0026plusmn; 6.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eWait circumference,cm\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e99.1 \u0026plusmn; 16.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e90.5 \u0026plusmn; 14.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e97.3 \u0026plusmn; 15.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e102.4 \u0026plusmn; 15.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e106.2 \u0026plusmn; 14.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eSBP, mmHg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e122.8 \u0026plusmn; 17.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e120.1 \u0026plusmn; 17.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e122.1 \u0026plusmn; 17.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e123.2 \u0026plusmn; 17.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e125.6 \u0026plusmn; 17.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eDBP, mmHg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e69.0 \u0026plusmn; 12.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e67.8 \u0026plusmn; 11.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e68.5 \u0026plusmn; 11.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e69.1 \u0026plusmn; 12.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e70.5 \u0026plusmn; 12.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eLaboratory Data\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eHemoglobin A1c (%)\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5.8 \u0026plusmn; 1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5.5 \u0026plusmn; 0.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5.6 \u0026plusmn; 0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5.8 \u0026plusmn; 1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6.1 \u0026plusmn; 1.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eFPG,mmol/L\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6.1 \u0026plusmn; 2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5.6 \u0026plusmn; 1.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5.8 \u0026plusmn; 1.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6.1 \u0026plusmn; 2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6.7 \u0026plusmn; 2.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eTG,mmol/L\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.2 (0.8, 1.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e0.6 (0.5, 0.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.0 (0.8, 1.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.4 (1.2, 1.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2.2 (1.8, 2.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eLDL-cholesterol ,mmol/L\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2.9 (2.3, 3.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2.6 (2.1, 3.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2.9 (2.3, 3.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e3.0 (2.5, 3.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e3.1 (2.4, 3.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eHDL-Cholesterol ,mmol/L\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.3 (1.1, 1.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.7 (1.4, 2.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.4 (1.2, 1.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.2 (1.1, 1.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.0 (0.9, 1.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eQuestionnaire Data\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eHypertension, n (%) Yes\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e3738 (35.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e680 (26.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e891 (33.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e978 (37.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1189 (45.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eDiabetes, n (%)Yes\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1293 (12.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e166 (6.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e255 (9.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e378 (14.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e494 (18.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eCVD, n (%)Yes\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e419 ( 4.0)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e81 (3.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e90 (3.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e103 (3.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e145 (5.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eSecondary calculated data\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eMAP, mmHg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e90.5 \u0026plusmn; 11.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e88.7 \u0026plusmn; 11.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e89.9 \u0026plusmn; 11.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e90.7 \u0026plusmn; 11.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e92.5 \u0026plusmn; 11.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eePWV,m/s\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e7.7 (6.4, 9.8)\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e7.1 (6.2, 9.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e7.6 (6.4, 9.7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e8.0 (6.6, 10.0)\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e8.1 (6.8, 9.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u0026lt; 0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003eAbbreviations:\u0026nbsp;\u003c/strong\u003eBMI, body mass index; PIR, family poverty income ratio; SBP,systolic blood pressure;DBP: diastolic blood pressure;, FBG: fasting blood glucose;TG,Triglyceride;CVD, Cardiovascular disease; MAP,mean arterial pressure;ePWV,Estimated pulse wave velocity;Quartile 1,VAI\u0026lt;0.87; Quartile 2, VAI(0.87-1.42); Quartile 3,VAI(1.42-2.41); Quartile 4,VAI\u0026gt;2.41.All statistical analyses in Table 1 were weighted using sample weights provided by NHANES to ensure that the results are nationally representative.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable2\u003c/strong\u003e\u0026nbsp; Linear multiple regression analysis model to assess the relationship between visceral adiposity indexs and estimated pulse wave velocity concentrations.\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"1035\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"2\" style=\"width: 190px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eVariable\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 209px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eModel 1\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 210px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eModel 2\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 213px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eModel 3\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 213px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eModel 4\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 145px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eCrude.Coefficient\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003e(95CI)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 64px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eP\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e-value\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 144px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eAdjusted.Coefficient\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003e(95CI)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eP\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e-value\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 146px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eAdjusted.Coefficient\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003e(95CI)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eP\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e-value\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 148px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eAdjusted.Coefficient\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003e(95CI)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eP\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e-value\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 190px;\"\u003e\n \u003cp\u003eVAI (0.2SD)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 145px;\"\u003e\n \u003cp\u003e0.1 (0.04,0.17)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 64px;\"\u003e\n \u003cp\u003e0.003\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 144px;\"\u003e\n \u003cp\u003e0.1 (0.03~0.17)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e0.004\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 146px;\"\u003e\n \u003cp\u003e0.09 (0.02~0.17)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e0.009\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 148px;\"\u003e\n \u003cp\u003e0.09 (0.02~0.16)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65px;\"\u003e\n \u003cp\u003e0.007\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 190px;\"\u003e\n \u003cp\u003eQuartile1 (\u0026lt;0.87)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 145px;\"\u003e\n \u003cp\u003e0(Ref)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 64px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 144px;\"\u003e\n \u003cp\u003e0(Ref)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 146px;\"\u003e\n \u003cp\u003e0(Ref)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 148px;\"\u003e\n \u003cp\u003e0(Ref)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 190px;\"\u003e\n \u003cp\u003eQuartile2 (0.87-1.42)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 145px;\"\u003e\n \u003cp\u003e0.31 (0.19~0.43)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 64px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 144px;\"\u003e\n \u003cp\u003e0.34 (0.22~0.46)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 146px;\"\u003e\n \u003cp\u003e0.3 (0.19~0.42)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 148px;\"\u003e\n \u003cp\u003e0.27 (0.16~0.38)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 190px;\"\u003e\n \u003cp\u003eQuartile3 (1.42-2.41)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 145px;\"\u003e\n \u003cp\u003e0.54 (0.42~0.65)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 64px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 144px;\"\u003e\n \u003cp\u003e0.6 1(0.48~0.72)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 146px;\"\u003e\n \u003cp\u003e0.53 (0.41~0.65)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 148px;\"\u003e\n \u003cp\u003e0.49 (0.37~0.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 190px;\"\u003e\n \u003cp\u003eQuartile4 (\u0026gt;2.41)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 145px;\"\u003e\n \u003cp\u003e0.62 (0.5~0.74)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 64px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 144px;\"\u003e\n \u003cp\u003e0.67 (0.56~0.79)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 146px;\"\u003e\n \u003cp\u003e0.61(0.48~0.72)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 148px;\"\u003e\n \u003cp\u003e0.53 (0.41~0.64)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 190px;\"\u003e\n \u003cp\u003eTrend.test\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 145px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 64px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 144px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 146px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 148px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65px;\"\u003e\n \u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003eAbbreviations:\u0026nbsp;\u003c/strong\u003eCI,Confidence Interval;PIR,Poverty income ratio; Model 1: unadjusted; \u0026nbsp;Model 2: adjusted for gender+race; Model 3: adjusted for Model 2+(educational level and PIR ); Model 4:adjusted for Model3+(Hypertension,Diabetes,Cardiovascular disease,Systolic blood pressure,Diastolic blood pressure,Smoking and Alcohol consumption)\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bmc-cardiovascular-disorders","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bcar","sideBox":"Learn more about [BMC Cardiovascular Disorders](http://bmccardiovascdisord.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bcar/default.aspx","title":"BMC Cardiovascular Disorders","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"obesity, arterial stiffness, visceral adiposity index, estimated pulse wave velocity, NHANES","lastPublishedDoi":"10.21203/rs.3.rs-5660240/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5660240/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eObesity and arterial stiffness are known risk factors for cardiovascular disease, but the relationship between visceral adiposity index (VAI) and estimated pulse wave velocity (ePWV) is still unclear.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eThe cross-sectional study used data from the 2007\u0026ndash;2016 survey cycle of the National Health and Nutrition Examination Survey (NHANES ) database. After data cleaning, the researchers conducted a comprehensive weighted statistical analysis of the final data set. This included data on demographics, medical history, tests, examinations and chronic comorbidities. The researchers used restricted cubic spline curves to examine potential linear associations between VAI and ePWV. In addition, weighted linear univariate and multivariate regression analyses were performed to further explore the potential relationship between VAI and ePWV. Finally, multiple subgroup analyses and interaction tests, as well as sensitivity analyses, were performed to test the stability of the relationship.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eFinally, 10458 adult participants aged 20 years or older were included in this study, with a mean (SD) age of 49.2\u0026thinsp;\u0026plusmn;\u0026thinsp;17.4 years after weighted analysis. Restricted cubic spline analysis showed a potentially \"inverted-L\" non-linear relationship between VAI and ePWV (\u003cem\u003eP\u003c/em\u003e for non-linearity: \u0026lt;0.001).The inflection point analysis suggests its inflection point is (1.48: 1.38\u0026ndash;1.57). Linear multivariate regression analysis suggested a significant positive correlation between VAI (0.2SD) and ePWV values (β\u0026thinsp;=\u0026thinsp;0.1, 95% CI 0.04\u0026ndash;0.17, p\u0026thinsp;\u0026lt;\u0026thinsp;0.001). The positive correlation between VAI (0.2SD) and ePWV levels remained stable in model analyses adjusted for all covariates. And in the highest quartile Q4 group, it showed a significant positive correlation compared to Q1 (β\u0026thinsp;=\u0026thinsp;0.53, 95% CI 0.41\u0026ndash;0.64, p\u0026thinsp;\u0026lt;\u0026thinsp;0.001). These findings remained stable and consistent in subsequent subgroup and sensitivity analyses.\u003c/p\u003e\u003ch2\u003eConclusions\u003c/h2\u003e \u003cp\u003eAccording to this study, elevated VAI levels are related to estimated pulse wave velocity, and there is an interaction between VAI and ePWV in populations that are aging, hypertensive, and diabetic.\u003c/p\u003e","manuscriptTitle":"The relationship between Visceral Adiposity Index and estimated Pulse Wave Velocity: Insights from NHANES Database","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-03-25 10:04:21","doi":"10.21203/rs.3.rs-5660240/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-04-08T18:28:28+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-04-08T18:24:23+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-04-07T08:43:54+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-04-02T09:09:17+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"82371362794356140012190509870935538575","date":"2025-03-25T16:07:47+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"266260854871394723271192837807126097770","date":"2025-03-25T08:37:29+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-03-24T11:29:17+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-03-22T02:49:10+00:00","index":"","fulltext":""},{"type":"submitted","content":"BMC Cardiovascular Disorders","date":"2025-03-21T13:32:12+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-cardiovascular-disorders","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bcar","sideBox":"Learn more about [BMC Cardiovascular Disorders](http://bmccardiovascdisord.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bcar/default.aspx","title":"BMC Cardiovascular Disorders","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"da00ff09-b9fe-412b-870d-12626b59f886","owner":[],"postedDate":"March 25th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2025-04-10T09:08:40+00:00","versionOfRecord":[],"versionCreatedAt":"2025-03-25 10:04:21","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-5660240","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-5660240","identity":"rs-5660240","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}