The Role Of Single-Cell RNA Sequencing In Cardiac Tumor - A Case Report

The Role Of Single-Cell RNA Sequencing In Cardiac Tumor - A Case Report | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The Role Of Single-Cell RNA Sequencing In Cardiac Tumor - A Case Report Xianjie Qi, Chao Shi, Ting Wei, Shengqiang Zhang, Jinjin Meng, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1364662/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: The benign cardiac tumor is a common disease. We often completely removed it by operation. However, the characteristic of the tumor is not studied deeply. Here, we used the technique of single-cell RNA sequencing to analyze cell composition and growth patterns in a giant cardiac tumor. Case presentation: A 65-year-old woman presented to our hospital with 5 day of chest tightness, dyspnea, lower abdominal distension. Echocardiography revealed a mass in the right atrium. An emergency operation was carried out to prevent tumor shedding. The single-cell RNA sequencing was applied to analysis the nature of the tumor. Conclusion: Single-cell RNA sequencing is a good manner to analyze the growth pattern and search for therapeutic targets. Based on the result of single-cell RNA sequencing, it is helpful to reduce the recurrence of the tumor and benefit the quality of life of patients. Single-cell RNA sequencing Cardiac tumor Case report Figures Figure 1 Figure 2 Figure 3 Background A cardiac tumor is a common disease. Treatment for the cardiac tumor is generally surgical resection, and show a good prognosis in benign tumor than a malignant one [ 1 ]. However, we often do not analyze the cell components and the cause of growth for tumors. Therefore, for a malignant tumor, surgical resection is not enough to obtain a satisfactory survival due to the lack of precision-targeted drugs. Recently, the technique of single-cell RNA sequencing is widely used [ 2 ]. It is helpful to understand the potential mechanism of the growth of the tumor. Herein, we report the application of single-cell sequencing in a giant cardiac tumor. Case Presentation A 65-year-old (62kg) female patient was admitted to our department in 2021. Her previous history was no special, but hypertension for 10 years. A mass in the right atrium was demonstrated on echocardiography. More seriously, the other mass was stuck in the tricuspid valve orifice. To prevent pulmonary embolism caused by tumor shedding, an emergency operation was carried out. Two masses were excised under cardiopulmonary bypass. The base of the larger tumor was located in the right atrial wall and filled the right atrium (Figure 1, A). The base of the smaller tumor was located above the tricuspid valve annulus, and the tumor crossed the tricuspid valve and penetrated deep into the right ventricle (Figure 1, B). The size was 3.0 × 7.0 × 7.0 cm 3 and 2.0 × 4.0 × 6.0 cm 3 , respectively (Figure 2, A). This patient recovered uneventfully, with no evidence of recurrence after 9 months of follow-up. The tumor was rich in cellular myxoma with chondroid metaplasia and sarcomatoid differentiation (Figure 2, B). Immunohistochemistry shown that Vimentin (+), Filami-1 (FIL-1) (+), Cluster of differentiation 99 (CD99) (+), Cluster of differentiation 34 (CD34) (+), Neuron specific enolase (NSE) (+), Casein kinase (CK) (-), S100 (-), Epithelial membrane antigen (EMA) (-), Cluster of differentiation 117 (CD117) (-), Specific AT sequence binding protein-2 (SATB-2) (-), proliferation index of Ki67 was 40%. To identify the features of tumor growth, we determined the cell composition and growth pattern of the tumor via single-cell RNA sequencing. Comparing with the public data in published studies [2], we found that the major cell components of this tumor were chondrocytes, mesenchymal stromal cells, and smooth muscle cells. After filtering, Gene Ontology (GO) analysis of 36 hub genes are involved in the multicellular organismal process, anatomical structure development, cellular response to stimulus, and cell differentiation, et al. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment showed that cyclic Guanosine monophosphate-cyclic - guanosine monophosphate (cGMP-PKG) signaling pathway, transcriptional misregulation in cancer, and p53 signaling pathway are related to the growth of the tumor. Protein-protein interaction of these genes was also analyzed (Figure 3). Discussion And Conclusions According to the classification of cardiac tumors from WHO in 2015 [3], myxomas and papillary fibroelastomas are the most common benign cardiac tumors, and are typically endocavitary. In our case, this tumor is classified into myxomas, although chondroid and sarcomatoid components were detected via immunohistochemistry. Surgery is the first choice of therapy for myxomas with a satisfactory long-term survival rate [1]. During the operation, we suspect that the tumor is malignant from its appearance. We chose two methods to detect its characteristics, one is pathological examination, the other is single-cell RNA sequencing. Luckily, the pathologic test revealed that the property of this tumor is benign. Meanwhile, the single-cell RNA sequencing is completed. From its results, we get much more information about this tumor. First, the components mainly include chondrocytes, smooth muscle cells, fibroblasts, mesenchymal stromal cells, osteoblasts. It echoes the result of the pathological examination. We do confirm that the tumor is benign by double confirmation. Secondly, through KEGG enrichment and protein-protein interaction of hub genes, we speculated that the causes of tumor growth were related to three signaling pathways, whose role in tumor has been studied widely [4-6]. Although it is a benign tumor in our case, benign or malignant tumor is a postoperative diagnosis that must keep in mind to select a proper treatment strategy. Currently, treatment and prognosis in malignant cardiac tumor are not satisfied 7 . Thus, it is necessary to improve the treatment plan, especially in postoperative strategy. We do believe that single-cell RNA sequencing is a good manner to analyze the growth pattern and search for therapeutic targets. Based on the result of single-cell RNA sequencing, seeking out a precision-targeted drug is helpful to reduce the recurrence of a malignant tumor and benefit the quality of life of patients. Abbreviations FIL: Filamin; CD: Cluster of differentiation; NSE: Neuron specific enolase; CK: Casein kinase; EMA: Epithelial membrane antigen; SATB: Specific AT sequence binding protein; cGMP-PKG: cyclic Guanosine monophosphate-cyclic - guanosine monophosphate Declarations Acknowledgments We sincerely thank Jialei Zheng, Lili Zhai, and Mingfang Zhang for their assistance in this work. Authors’ contributions Xian-jie Qi -Writing, Statistics and Draft. Chao Shi - Writing and Draft. Wei Ting -Data analysis. Sheng-qiang Zhang -Data analysis. Jin-jin Meng -Statistics. Yi-yao Jiang -Design, Reviewing. The authors read and approved the final manuscript. Funding This work was supported by grants from the National Natural Science Foundation of China (81800214), Excellent Young Talents Fund Program of Higher Education Institutions of Anhui Province (gxyqZD2016167), Key Research and Development Projects of Anhui Province (1804h08020280), Graduate scientific research innovation program of Bengbu Medical College (Byycx21077). Availability of data and materials Not applicable. Ethics approval and consent to participate Not applicable. Consent for publication The patient provided written, informed consent. Competing interests The authors declare that they have no competing interests Author details 1 Department of Cardiovascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China. References Shah IK, et al Cardiac Myxomas: A 50-Year Experience With Resection and Analysis of Risk Factors for Recurrence. Ann Thorac Surg. 2015;100(2): 495–500. https://doi: 10.1016/j.athoracsur.2015.03.007 . Tucker NR, et al. Transcriptional and Cellular Diversity of the Human Heart. Circulation. 2020;142(5):466–82. doi. 10.1161/CIRCULATIONAHA.119.045401. Burke A. Tavora F. The 2015 WHO Classification of Tumors of the Heart and Pericardium. J Thorac Oncol. 2016; 11(4): 441 – 52. http:// doi : 10.1016/j.jtho.2015.11.009 . Lv Y, et al Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. PLoS Biol. 2020;18(11):e3000872. http:// doi : 10.1371/journal.pbio.3000872 . Lee TI. Young RA. Transcriptional regulation and its misregulation in disease. Cell. 2013;152(6):1237–51. http:// doi : 10.1016/j.cell.2013.02.014 . Stegh AH. Targeting the p53 signaling pathway in cancer therapy - the promises, challenges and perils. Expert Opin Ther Targets. 2012;16(1):67–83. doi. 10.1517/14728222.2011.643299. Antwi-Amoabeng D, et al. Survival Differences in Men and Women With Primary Malignant Cardiac Tumor: An Analysis Using the Surveillance, Epidemiology and End Results (SEER) Database From 1973 to 2015. J Am Heart Assoc. 2020;9(10):e014846. doi. 10.1161/JAHA.119.014846. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1364662","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":85788982,"identity":"86a9307e-993b-4df7-9001-0106133a5c2d","order_by":0,"name":"Xianjie Qi","email":"","orcid":"","institution":"The First Affiliated Hospital of Bengbu Medical College","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Xianjie","middleName":"","lastName":"Qi","suffix":""},{"id":85788983,"identity":"e0a0e6df-6bc6-457f-9b42-6fd9ea22619b","order_by":1,"name":"Chao Shi","email":"","orcid":"","institution":"The First Affiliated Hospital of Bengbu Medical College","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Chao","middleName":"","lastName":"Shi","suffix":""},{"id":85788984,"identity":"bb25fe80-705b-45af-8152-513f525d174e","order_by":2,"name":"Ting Wei","email":"","orcid":"","institution":"The First Affiliated Hospital of Bengbu Medical College","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Ting","middleName":"","lastName":"Wei","suffix":""},{"id":85788985,"identity":"fd2c3265-8b06-4f31-ad13-174b9b3156aa","order_by":3,"name":"Shengqiang Zhang","email":"","orcid":"","institution":"The First Affiliated Hospital of Bengbu Medical College","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Shengqiang","middleName":"","lastName":"Zhang","suffix":""},{"id":85788986,"identity":"add10346-0f40-4464-aa6c-60f47d1d99ae","order_by":4,"name":"Jinjin Meng","email":"","orcid":"","institution":"The First Affiliated Hospital of Bengbu Medical College","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jinjin","middleName":"","lastName":"Meng","suffix":""},{"id":85788987,"identity":"b528d481-e0b4-4deb-9f98-1e9419107dd9","order_by":5,"name":"Yiyao Jiang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA+0lEQVRIiWNgGAWjYBACxmYwJQFmPkiosGFgI0EL8zGDD2fSCGtBAmwJkjPbDhNWx9zO/Ozh1zaLxP72HgNj3rbz9nzSzQ8YflRsw+MwNnNj2TaJxBlnzhg85jl3O7FN5pgBY8+Z2/j8YiYtCdTScCPHwJin7HYCm0SCATNjGz4t7N/AWuYDtUjzsJ2zZ5NI/0BAC4+Z5Eeglg030hIkZ7QdYGyTyCFkC0+ZNMM5CeONZw6DAjk5Eail4CA+vxj2H98m+aOsTnbe8UZQVNrZy89I3/jgRwUeLQ3AgOZlY3BsQBY9gFM9EMiDHPfjD4M9PkWjYBSMglEwwgEADMhZkY4ZQwwAAAAASUVORK5CYII=","orcid":"","institution":"The First Affiliated Hospital of Bengbu Medical College","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Yiyao","middleName":"","lastName":"Jiang","suffix":""}],"badges":[],"createdAt":"2022-02-16 08:26:39","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-1364662/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1364662/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":18588616,"identity":"2aa4d216-dcc0-4acd-82eb-21bd0d53b84b","added_by":"auto","created_at":"2022-02-24 22:02:45","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":1643327,"visible":true,"origin":"","legend":"\u003cp\u003eA The larger tumor was located in the right atrial wall and filled the right atrium.\u003c/p\u003e\u003cp\u003eB The base of the smaller tumor was located above the tricuspid valve annulus.\u003c/p\u003e","description":"","filename":"Figure1.png","url":"https://assets-eu.researchsquare.com/files/rs-1364662/v1/9bfd2a8260dab1732cf9843e.png"},{"id":18588615,"identity":"2bddfeee-f92c-4dfe-a744-c28137a19f2d","added_by":"auto","created_at":"2022-02-24 22:02:45","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":1293877,"visible":true,"origin":"","legend":"\u003cp\u003eA Photograph shows a macroscopic view of the tumor, which were 3.0 × 7.0 × 7.0 cm\u003csup\u003e3\u003c/sup\u003e and 2.0 × 4.0 × 6.0 cm\u003csup\u003e3\u003c/sup\u003e, respectively.\u003c/p\u003e\u003cp\u003eB Immunohistochemistry detected tumor was rich in cellular myxoma with chondroid metaplasia and sarcomatoid differentiation.\u003c/p\u003e","description":"","filename":"Figure2.png","url":"https://assets-eu.researchsquare.com/files/rs-1364662/v1/f789c9575eba9c76f85a9de9.png"},{"id":18588617,"identity":"8ad3bdea-98bb-4689-880f-479e8e8c18d8","added_by":"auto","created_at":"2022-02-24 22:02:45","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":2035517,"visible":true,"origin":"","legend":"\u003cp\u003eA Cell subgroups and cell numbers.\u003c/p\u003e\u003cp\u003eB Dimension Reduction Clustering grouping display between heart tumor and normal heart.\u003c/p\u003e\u003cp\u003eC Cell type diagram.\u003c/p\u003e\u003cp\u003eD Heat map of cell type identification correlation.\u003c/p\u003e\u003cp\u003eE Protein-protein interaction of 36 hub genes.\u003c/p\u003e","description":"","filename":"Figure3.png","url":"https://assets-eu.researchsquare.com/files/rs-1364662/v1/6b4983074486c9ec99a384e6.png"},{"id":18588619,"identity":"f8d73f6b-15b9-4d2a-9f74-3637cb161b72","added_by":"auto","created_at":"2022-02-24 22:02:48","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":209254,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1364662/v1/d6fbe9d1-967f-4ac1-85a4-72b3dfcc61fb.pdf"},{"id":18588618,"identity":"126c8267-5d81-4fb8-9c1f-ec91589eb25f","added_by":"auto","created_at":"2022-02-24 22:02:48","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":208371,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1364662/v1/0a0b9a2f-0ef7-405c-ad87-37c07cdfe171.pdf"}],"financialInterests":"","formattedTitle":"\u003cp\u003eThe Role Of Single-Cell RNA Sequencing In Cardiac Tumor - A Case Report\u003c/p\u003e","fulltext":[{"header":"Background","content":"\u003cp\u003eA cardiac tumor is a common disease. Treatment for the cardiac tumor is generally surgical resection, and show a good prognosis in benign tumor than a malignant one [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. However, we often do not analyze the cell components and the cause of growth for tumors. Therefore, for a malignant tumor, surgical resection is not enough to obtain a satisfactory survival due to the lack of precision-targeted drugs. Recently, the technique of single-cell RNA sequencing is widely used [\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]. It is helpful to understand the potential mechanism of the growth of the tumor. Herein, we report the application of single-cell sequencing in a giant cardiac tumor.\u003c/p\u003e"},{"header":"Case Presentation","content":"\u003cp\u003eA 65-year-old (62kg) female patient was admitted to our department in 2021. Her previous history was no special, but hypertension for 10 years. A mass in the right atrium was demonstrated on echocardiography. More seriously, the other mass was stuck in the tricuspid valve orifice. To prevent pulmonary embolism caused by tumor shedding, an emergency operation was carried out.\u003c/p\u003e\n\u003cp\u003eTwo masses were excised under cardiopulmonary bypass. The base of the larger tumor was located in the right atrial wall and filled the right atrium (Figure 1, A). The base of the smaller tumor was located above the tricuspid valve annulus, and the tumor crossed the tricuspid valve and penetrated deep into the right ventricle (Figure 1, B). The size was 3.0 \u0026times; 7.0 \u0026times; 7.0 cm\u003csup\u003e3\u003c/sup\u003e and 2.0 \u0026times; 4.0 \u0026times; 6.0 cm\u003csup\u003e3\u003c/sup\u003e, respectively (Figure 2, A). This patient recovered uneventfully, with no evidence of recurrence after 9 months of follow-up.\u003c/p\u003e\n\u003cp\u003eThe tumor was rich in cellular myxoma with chondroid metaplasia and sarcomatoid differentiation (Figure 2, B). Immunohistochemistry shown that Vimentin (+), Filami-1 (FIL-1) (+), Cluster of differentiation 99 (CD99) (+), Cluster of differentiation 34 (CD34) (+), Neuron specific enolase (NSE) (+), Casein kinase (CK) (-), S100 (-), Epithelial membrane antigen (EMA) (-), Cluster of differentiation 117 (CD117) (-), Specific AT sequence binding protein-2 (SATB-2) (-), proliferation index of Ki67 was 40%.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eTo identify the features of tumor growth, we determined the cell composition and growth pattern of the tumor via single-cell RNA sequencing. Comparing with the public data in published studies [2], we found that the major cell components of this tumor were chondrocytes, mesenchymal stromal cells, and smooth muscle cells. After filtering, Gene Ontology (GO) analysis of 36 hub genes are involved in the multicellular organismal process, anatomical structure development, cellular response to stimulus, and cell differentiation, et al. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment showed that cyclic Guanosine monophosphate-cyclic - guanosine monophosphate (cGMP-PKG) signaling pathway, transcriptional misregulation in cancer, and p53 signaling pathway are related to the growth of the tumor. Protein-protein interaction of these genes was also analyzed (Figure 3).\u0026nbsp;\u003c/p\u003e"},{"header":"Discussion And Conclusions","content":"\u003cp\u003eAccording to the classification of cardiac tumors from WHO in 2015 [3], myxomas and papillary fibroelastomas are the most common benign cardiac tumors, and are typically endocavitary. In our case, this tumor is classified into myxomas, although chondroid and sarcomatoid components were detected via immunohistochemistry. Surgery is the first choice of therapy for myxomas with a satisfactory long-term survival rate [1].\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eDuring the operation, we suspect that the tumor is malignant from its appearance. We chose two methods to detect its characteristics, one is pathological examination, the other is single-cell RNA sequencing. Luckily, the pathologic test revealed that the property of this tumor is benign. Meanwhile, the single-cell RNA sequencing is completed. From its results, we get much more information about this tumor. First, the components mainly include chondrocytes, smooth muscle cells, fibroblasts, mesenchymal stromal cells, osteoblasts. It echoes the result of the pathological examination. We do confirm that the tumor is benign by double confirmation. Secondly, through KEGG enrichment and protein-protein interaction of hub genes, we speculated that the causes of tumor growth were related to three signaling pathways, whose role in tumor has been studied widely [4-6].\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eAlthough it is a benign tumor in our case, benign or malignant tumor is a postoperative diagnosis that must keep in mind to select a proper treatment strategy. Currently, treatment and prognosis in malignant cardiac tumor are not satisfied\u003csup\u003e7\u003c/sup\u003e. Thus, it is necessary to improve the treatment plan, especially in postoperative strategy. We do believe that single-cell RNA sequencing is a good manner to analyze the growth pattern and search for therapeutic targets. Based on the result of single-cell RNA sequencing, seeking out a precision-targeted drug is helpful to reduce the recurrence of a malignant tumor and benefit the quality of life of patients.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eFIL: Filamin; CD: Cluster of differentiation; NSE: Neuron specific enolase; CK: Casein kinase; EMA: Epithelial membrane antigen; SATB: Specific AT sequence binding protein; cGMP-PKG: cyclic Guanosine monophosphate-cyclic - guanosine monophosphate\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgments\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe sincerely thank Jialei Zheng, Lili Zhai, and Mingfang Zhang for their assistance in this work.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026rsquo; contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eXian-jie Qi -Writing, Statistics and Draft. Chao Shi - Writing and Draft. Wei Ting -Data analysis. Sheng-qiang Zhang -Data analysis. Jin-jin Meng -Statistics. Yi-yao Jiang -Design, Reviewing. The authors read and approved the final manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis work was supported by grants from the National Natural Science Foundation of China (81800214), Excellent Young Talents Fund Program of Higher Education Institutions of Anhui Province (gxyqZD2016167), Key Research and Development Projects of Anhui Province (1804h08020280), Graduate scientific research innovation program of Bengbu Medical College (Byycx21077).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe patient provided written, informed consent.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that they have no competing interests\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor details\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003csup\u003e1\u003c/sup\u003eDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eShah IK, et al Cardiac Myxomas: A 50-Year Experience With Resection and Analysis of Risk Factors for Recurrence. Ann Thorac Surg. 2015;100(2): 495\u0026ndash;500. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi: 10.1016/j.athoracsur.2015.03.007\u003c/span\u003e\u003c/span\u003e.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTucker NR, et al. Transcriptional and Cellular Diversity of the Human Heart. Circulation. 2020;142(5):466\u0026ndash;82. \u003cem\u003edoi. 10.1161/CIRCULATIONAHA.119.045401.\u003c/em\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eBurke A. Tavora F. \u003cem\u003eThe 2015 WHO Classification of Tumors of the Heart and Pericardium. J Thorac Oncol.\u003c/em\u003e 2016;\u003cem\u003e11(4): 441 \u0026ndash; 52.\u003c/em\u003e \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://\u003c/span\u003e\u003c/span\u003e\u003cem\u003edoi\u003c/em\u003e: \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.jtho.2015.11.009\u003c/span\u003e\u003c/span\u003e.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLv Y, et al Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. PLoS Biol. 2020;18(11):e3000872. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://\u003c/span\u003e\u003c/span\u003e\u003cem\u003edoi\u003c/em\u003e: \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1371/journal.pbio.3000872\u003c/span\u003e\u003c/span\u003e.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLee TI. Young RA. Transcriptional regulation and its misregulation in disease. Cell. 2013;152(6):1237\u0026ndash;51. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://\u003c/span\u003e\u003c/span\u003e\u003cem\u003edoi\u003c/em\u003e: \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.cell.2013.02.014\u003c/span\u003e\u003c/span\u003e.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eStegh AH. Targeting the p53 signaling pathway in cancer therapy - the promises, challenges and perils. Expert Opin Ther Targets. 2012;16(1):67\u0026ndash;83. \u003cem\u003edoi. 10.1517/14728222.2011.643299.\u003c/em\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAntwi-Amoabeng D, et al. Survival Differences in Men and Women With Primary Malignant Cardiac Tumor: An Analysis Using the Surveillance, Epidemiology and End Results (SEER) Database From 1973 to 2015. J Am Heart Assoc. 2020;9(10):e014846. \u003cem\u003edoi. 10.1161/JAHA.119.014846.\u003c/em\u003e\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Single-cell RNA sequencing, Cardiac tumor, Case report","lastPublishedDoi":"10.21203/rs.3.rs-1364662/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1364662/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground:\u003c/strong\u003e The benign cardiac tumor is a common disease. We often completely removed it by operation. However, the characteristic of the tumor is not studied deeply. Here, we used the technique of single-cell RNA sequencing to analyze cell composition and growth patterns in a giant cardiac tumor. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eCase presentation: \u003c/strong\u003eA 65-year-old woman presented to our hospital with 5 day of chest tightness, dyspnea, lower abdominal distension. Echocardiography revealed a mass in the right atrium. An emergency operation was carried out to prevent tumor shedding. The single-cell RNA sequencing was applied to analysis the nature of the tumor.\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eConclusion: \u003c/strong\u003eSingle-cell RNA sequencing is a good manner to analyze the growth pattern and search for therapeutic targets. Based on the result of single-cell RNA sequencing, it is helpful to reduce the recurrence of the tumor and benefit the quality of life of patients.\u003c/p\u003e","manuscriptTitle":"The Role Of Single-Cell RNA Sequencing In Cardiac Tumor - A Case Report","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-02-24 22:02:43","doi":"10.21203/rs.3.rs-1364662/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"ce5eab79-14d7-484c-9d98-6b7d765b8955","owner":[],"postedDate":"February 24th, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2022-02-24T22:02:45+00:00","versionOfRecord":[],"versionCreatedAt":"2022-02-24 22:02:43","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-1364662","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-1364662","identity":"rs-1364662","version":["v1"]},"buildId":"7rjqhiLT3MXkJMwkYKINL","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}