The Role of Neurogenic Bioamines in Nerve Fibers of Uterus during the Postpartum Involution in Experimental Animal Models

Sergey V. Dindyaev, Dindyaev SV, Narasimha M. Beeraka, Kasatkin DV, Denis V. Kasatkin, Elizaveta V. Mikhaylenko, Somasundaram SG, Siva G. Somasundaram, Cecil E. Kirkland, Kirkland CE, Gjumrakch Aliev, Aliev G
Current pharmaceutical design · 2021 · vol. 27(27) , pp. 3061–3073 · doi:10.2174/1381612827666210322141205 · PMID:33749552 · W3136637441
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This study quantified biogenic amine concentrations in rat uterine nerve fibers and mast cells during postpartum involution to assess their role in uterine recovery and reproductive health.

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This experimental study investigated the density and spatial distribution of sympathetic nerve fibers and biogenic amines in the uteri of 55 postpartum rats to understand their role in uterine involution. Using fluorescence histochemistry, researchers measured serotonin and catecholamine concentrations in perivascular plexuses and single sympathetic nerve terminals across different days after parturition. The results indicated that biogenic amine levels peaked on the tenth day, with significant variations in nerve fiber density between the uterine body, cervix, and mesentery. The authors concluded that a balanced ratio of these antagonistic amines is essential for maintaining the anabolic-catabolic equilibrium necessary for proper uterine recovery. Relevance to endometriosis: the paper explicitly cites endometriosis and adenomyosis as conditions linked to imbalances in biogenic amines and delayed uterine involution, though its primary focus remains on postpartum physiological changes in rat models.

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Abstract

BACKGROUND: Biogenic amines (BAs) secreted by the sympathetic neural apparatus of rat uterus are reported to be conducive to the uterine functional activity during postpartum involution; the imbalance in BAs ratio could confer postpartum reproductive disorders including improper postpartum involution. OBJECTIVE: The objective of this study is to identify the changes in the density of uterine sympathetic nerves implicated in the pathology of endometriosis, adenomyosis, and delayed uterine involution. The present study aims to ascertain 'serotonin' and 'catecholamine' concentrations in mesenteric mast cells (MCs), and structural elements of nerve fibers across the perivascular plexuses (PPs) and single sympathetic nerve terminals (SST). METHODS: Furthermore, the density of their spatial distribution (SDP and SDT) in the uterine body, cervix, and mesometrium was determined during postpartum involution. Tissue specimens of postpartum uterus were obtained from 55 nulliparous female Wistar outbred strain rats, which were grouped according to the days after parturition at the time of sacrifice. The nerve fibers of PP and SST exhibited emerald green fluorescence, which was detected by glyoxylic acid fluorescence technique; the fluorescence invoked by BAs was identified by microspectrofluorimetry. RESULTS: Concentrations of BAs were extensive in the varicosities of PP and SST on the 10th day. However, the highest BA concentrations were found in structural elements of PP in the uterine mesometrium in the initial days of postpartum. In mesenteric MC, serotonin and catecholamines were at the highest concentration on the 10th day of postpartum. Histamines peaked on the 6th day. CONCLUSION: SDP and SDT were increased significantly in all structural elements of uterine nerve fibers in the uterine body and cervix compared to SDP in mesentery. Considering that catecholamines and serotonin are antagonists in many aspects of their biological action, the ratio of BAs should be well-balanced to maintain anabolic- catabolic equilibrium in the rat uterus.
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Abstract

Background: Biogenic amines (BAs) secreted by the sympathetic neural apparatus of rat uterus are reported to be conducive to the uterine functional activity during postpartum involution; the imbalance in BAs ratio could confer postpartum reproductive disorders including improper postpartum involution.

Objective

The objective of this study is to identify the changes in the density of uterine sympathetic nerves implicated in the pathology of endometriosis, adenomyosis, and delayed uterine involution. The present study aims to ascertain ‘serotonin’ and ‘catecholamine’ concentrations in mesenteric mast cells (MCs), and structural elements of nerve fibers across the perivascular plexuses (PPs) and single sympathetic nerve terminals (SST).

Methods

Furthermore, the density of their spatial distribution (SDP and SDT) in the uterine body, cervix, and mesometrium was determined during postpartum involution. Tissue specimens of postpartum uterus were obtained from 55 nulliparous female Wistar outbred strain rats, which were grouped according to the days after parturition at the time of sacrifice. The nerve fibers of PP and SST exhibited emerald green fluorescence, which was detected by glyoxylic acid fluorescence technique; the fluorescence invoked by BAs was identified by microspectrofluorimetry.

Results

Concentrations of BAs were extensive in the varicosities of PP and SST on the 10th day. However, the highest BA concentrations were found in structural elements of PP in the uterine mesometrium in the initial days of postpartum. In mesenteric MC, serotonin and catecholamines were at the highest concentration on the 10th day of postpartum. Histamines peaked on the 6th day.

Conclusion

SDP and SDT were increased significantly in all structural elements of uterine nerve fibers in the uterine body and cervix compared to SDP in mesentery. Considering that catecholamines and serotonin are antagonists in many aspects of their biological action, the ratio of BAs should be well-balanced to maintain anabolic- catabolic equilibrium in the rat uterus.

Keywords

Catecholamines, fluorescence histochemistry, serotonin, sympathetic nerve fibers, uterine mesometrium, SDP, SDT.

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Condition tags

endometriosisadenomyosis

MeSH descriptors

Postpartum Period Uterus Animals Female Humans Models, Animal Nerve Fibers Rats Rats, Wistar

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