Phylogenetics of rice (Oryza sativa L.) genotypes from Pakistan based on diversity of biochemical markers

In this study phylogenetics and diversity of 150 rice ( Oryza sativa L.) genotypes originated from different regions of an agricultural country Pakistan, self-sufficient in its production were analyzed. Total seed protein profile of these genotypes was assessed using a technique of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The seeds of accessions from different geographical regions of the country were provided by the Institute of Agri-Biotechnology and Genetic Resources, National Agricultural Research Centre (IABGRI-NARC) Islamabad, Pakistan. Total endosperm proteins from each genotype were extracted and used as biochemical markers. Three different protein extraction protocols were followed in comparison to optimize the procedure. An immense content of genetic variability was confirmed based on polymorphism data of polypeptide bands. The Jaccard’s dissimilarity coefficient ranged from 0.09–0.94 for all 150 accessions. Based on the neighbour joining method of clustering, the whole germplasm was divided into two clusters, each further subdivided sorting centre of origin and depicting diversity. In the same manner, 126 accessions of province Khyber Pakhtunkhwa (KPK) were also assessed separately. This wealth of knowledge based on proteins profiling will be used for marker assisted breeding/selection, germplasm conservation strategies and easy identification of these genotypes from Pakistan.