Role of cell wall mutation in antimicrobial resistance in Escherichia coli H7:O157: A molecular evolution study Antimicrobial resistance associated with cell wall change

Role of cell wall mutation in antimicrobial resistance in Escherichia coli H7:O157: A molecular evolution study Antimicrobial resistance associated with cell wall change | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 14 January 2025 V1 Latest version Share on Role of cell wall mutation in antimicrobial resistance in Escherichia coli H7:O157: A molecular evolution study Antimicrobial resistance associated with cell wall change Authors : Walaa A. Sallomi , Ikram Abbas Abbood , and Rebah N. Algafari 0000-0001-8032-3640 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.173682011.14821917/v1 171 views 71 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The E. coli H7:O157 was the subject of many studies. It is characterized by producing Shiga toxins, abdominal illness, and developing resistance to antimicrobial agents. A total 20 fecal samples tested positive for E. coli H7:O157 from patients showed the typical symptoms of the infection, and another 20 from animals were collected. The bacterium was isolated, identified, and classified using culture medium, and molecular methods. Results obtained from classification of E. coli H7:O157 showed it is highly similar to E. coli H7:O157 strain Sakai. The genes rpo B, stx , waa , and rfb O were deposited in the NCBI website under accession no. PP059841, OR939814, PP059843, and PP059842 respectively. The mutant sequences of waa sites K, L, Y were analyzed to determine the shifting in their function, cell wall formation, and the ability to develop antibiotic resistance the mutant E. coli H7:O157 compared to the wild type. We found that the resistance to antibiotics in mutant E. coli H7:O157 increased significantly when compared to the wild type. Animals. The explanation for this may be attributed to the waa K, and waa L by preventing theentry of antimicrobial agents into the bacterial cell. Supplementary Material File (role of cell wall mutation in antimicrobial resistance final.docx) Download 3.42 MB Information & Authors Information Version history V1 Version 1 14 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords e. coli h7:o157 bacterial cell wall modification microbial pathogenesis site directed mutagenesis Authors Affiliations Walaa A. Sallomi Purdue University College of Veterinary Medicine View all articles by this author Ikram Abbas Abbood Purdue University College of Veterinary Medicine View all articles by this author Rebah N. Algafari 0000-0001-8032-3640 [email protected] Al-Nahrain University Biotechnology Research Center View all articles by this author Metrics & Citations Metrics Article Usage 171 views 71 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Walaa A. Sallomi, Ikram Abbas Abbood, Rebah N. Algafari. Role of cell wall mutation in antimicrobial resistance in Escherichia coli H7:O157: A molecular evolution study Antimicrobial resistance associated with cell wall change. Authorea . 14 January 2025. DOI: https://doi.org/10.22541/au.173682011.14821917/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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