Grapevine protection against Plasmopara viticola by a natural extract involves a signaling cascade common to Rpv locus-mediated defense responses

Grapevine protection against Plasmopara viticola by a natural extract involves a signaling cascade common to Rpv locus-mediated defense responses | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 16 January 2026 V1 Latest version Share on Grapevine protection against Plasmopara viticola by a natural extract involves a signaling cascade common to Rpv locus-mediated defense responses Authors : Giacomo Manganotti , Davide Danzi , Nicola Vitulo , Cristina Sudiro , Annalisa Polverari 0000-0003-2963-5731 , Adriano Altissimo , and Elodie Vandelle 0000-0002-4205-6331 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.176855627.71791722/v1 106 views 57 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The cultivation of Vitis vinifera is severely constrained by fungal and oomycete diseases that negatively impact yield and fruit quality. Although phytosanitary products remain central to disease control, their intensive use raises major concerns related to pathogen resistance, environmental impact, human health, and increasingly strict regulatory constraints. Consequently, sustainable and integrated disease management strategies that reduce chemical inputs while maintaining high production standards are urgently needed. In this context, a plant-derived natural extract (LL017) has been developed as a sustainable plant protection product, demonstrating good field efficacy with a reduction of downy mildew ( Plasmopara viticola ) severity when applied in combination with only 30% of the standard fungicide dose. This highlights its strong potential for integration into reduced-input protection strategies. To support the rational deployment of biologicals and to generate new mechanistic knowledge, we investigated the molecular mode of action underlying LL017-induced resistance in grapevine. LL017 did not directly affect pathogen viability but instead activated host-mediated defence responses, inducing coordinated early and late immune mechanisms comparable to those controlled by well-characterized grapevine resistance loci. Deep metatranscriptomic analyses and gene co-expression network inference identified an early signalling cascade converging on stilbene synthase regulation as a key component of LL017-mediated resistance. In parallel, LL017 modulated primary metabolism–related genes, suggesting a broader transcriptional reprogramming associated with enhanced grapevine fitness. Overall, this study provides new fundamental insights into the molecular networks underlying robust defence responses in grapevine while supporting the optimized use of biologicals in sustainable disease management strategies. Supplementary Material File (manganotti et al_manuscript_pce_v2.docx) Download 167.69 KB Information & Authors Information Version history V1 Version 1 16 January 2026 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords downy mildew grapevine molecular defenses grapevine resistance signaling transcriptional reprogramming transcriptome Authors Affiliations Giacomo Manganotti Universita degli Studi di Verona Dipartimento di Biotecnologie View all articles by this author Davide Danzi Universita degli Studi di Verona Dipartimento di Biotecnologie View all articles by this author Nicola Vitulo Universita degli Studi di Verona Dipartimento di Biotecnologie View all articles by this author Cristina Sudiro Landlab srl View all articles by this author Annalisa Polverari 0000-0003-2963-5731 Universita degli Studi di Verona Dipartimento di Biotecnologie View all articles by this author Adriano Altissimo Landlab srl View all articles by this author Elodie Vandelle 0000-0002-4205-6331 [email protected] Universita degli Studi di Verona Dipartimento di Biotecnologie View all articles by this author Metrics & Citations Metrics Article Usage 106 views 57 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Giacomo Manganotti, Davide Danzi, Nicola Vitulo, et al. Grapevine protection against Plasmopara viticola by a natural extract involves a signaling cascade common to Rpv locus-mediated defense responses. Authorea . 16 January 2026. DOI: https://doi.org/10.22541/au.176855627.71791722/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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