Arterial pulsations and transmantle pressure synergetically drive glymphatic flow

Arterial pulsations and transmantle pressure synergetically drive glymphatic flow | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Arterial pulsations and transmantle pressure synergetically drive glymphatic flow Guillermo L. Nozaleda, Wilfried Coenen, Victor Haughton, Antonio L. Sánchez This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5257018/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 21 Apr, 2025 Read the published version in Scientific Reports → Version 1 posted 11 You are reading this latest preprint version Abstract Clearance of waste material from the brain by the glymphatic system results from net flow of cerebrospinal fluid (CSF) through perivascular spaces surrounding veins and arteries. In periarterial spaces, this bulk flow is directed from the cranial subarachnoid space towards the brain’s interior. The precise pumping mechanism explaining this net inflow remains unclear. While in vivo experiments have shown that the pulsatile motion in periarterial spaces is synchronized with arterial pulsations, peristalsis alone has been deemed insufficient to explain bulk flow. In this study we examine an alternative mechanism based on the interaction between arterial pulsations and fluctuations in transmantle pressure. Previously studied using pressure data from a hydrocephalus patient, this mechanism is analyzed here in healthy subjects using in vivo flow measurements obtained via phase-contrast magnetic resonance imaging. Arterial pulsations are derived from flow-rate measurements of arterial blood entering the cranial cavity, while transmantle-pressure fluctuations are computed using measurements of CSF flow in the cerebral aqueduct. The two synchronized waveforms are integrated into a canonical multi-branch model of the periarterial spaces, yielding a closed-form expression for the bulk flow. The results confirm that the dynamic interactions between arterial pulsations and transmantle pressure are sufficient to generate a positive inflow along periarterial spaces. Physical sciences/Physics/Fluid dynamics Physical sciences/Physics/Biological physics Biological sciences/Neuroscience Physical sciences/Engineering/Biomedical engineering Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 21 Apr, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 14 Feb, 2025 Reviews received at journal 12 Feb, 2025 Reviewers agreed at journal 31 Jan, 2025 Reviews received at journal 27 Nov, 2024 Reviewers agreed at journal 18 Nov, 2024 Reviewers agreed at journal 17 Nov, 2024 Reviewers invited by journal 17 Nov, 2024 Editor assigned by journal 12 Nov, 2024 Editor invited by journal 08 Nov, 2024 Submission checks completed at journal 08 Nov, 2024 First submitted to journal 13 Oct, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5257018","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":375739195,"identity":"e33d8d3a-9a3c-455c-b2c5-473d2615319f","order_by":0,"name":"Guillermo L. 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In periarterial spaces, this bulk flow is directed from the cranial subarachnoid space towards the brain’s interior. The precise pumping mechanism explaining this net inflow remains unclear. While in vivo experiments have shown that the pulsatile motion in periarterial spaces is synchronized with arterial pulsations, peristalsis alone has been deemed insufficient to explain bulk flow. In this study we examine an alternative mechanism based on the interaction between arterial pulsations and fluctuations in transmantle pressure. Previously studied using pressure data from a hydrocephalus patient, this mechanism is analyzed here in healthy subjects using in vivo flow measurements obtained via phase-contrast magnetic resonance imaging. Arterial pulsations are derived from flow-rate measurements of arterial blood entering the cranial cavity, while transmantle-pressure fluctuations are computed using measurements of CSF flow in the cerebral aqueduct. 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