Endometriose peritoneal experimental em ratas: avaliação da dor, fertilidade, estresse oxidativo, inflamação e sua resposta aos nutracêuticos ômega-6/3 e ômega-9/6

Endometriosis is defined as the presence of endometrial tissue (glands and stroma) \noutside the uterine cavity. Objective: Evaluate the effect of the nutraceuticals \nomega-6/3 and omega-9/6 on pain, fertility, inflammation and oxidative stress \nassociated with endometriosis. Methods: In this controlled experimental study, \nperitoneal endometriosis was induced surgically in albino Wistar rats by implanting \ntwo fragments from the right hemiuterus, one near the right ovary and one in the \nanterior abdominal wall. The distribution of wet and dry weight was evaluated at four \nmoments (days 1, 7, 14 and 21). Groups of 8 animals were submitted to treatment \nwith either β-stradiol 10 mg/kg/day, medroxyprogesterone acetate 5 mg/kg every 3 \ndays, omega-6/3 1.2 g/kg/day, omega-9/6 1.2 g/kg/day, or meloxicam 0.8 mg/kg/day, \nand compared with two control groups (sham surgery, and endometriosis + 0.9% \nsaline). Pain was quantified with the abdominal contortion test for the two control \ngroups, the omega-6/3 group, the omega-9/6 group, the medroxyprogesterone \nacetate group and the meloxicam group, at the described dosages. Fertility was \nexpressed as the number of developing embryos in the left hemiuterus for the two \ncontrol groups, the omega-6/3 group, the omega-9/6 group and the meloxicam \ngroup, at the described dosages. Inflammation was proxied by the plasma level of Creactive \nprotein for the two control groups, the omega-6/3 group and the omega-9/6 \ngroup, at the described dosages. Oxidative stress was represented by plasma and \ntissue glutathione and TBARS levels for the two control groups, the omega-6/3 group \nand the omega-9/6 group, at the described dosages. Results: The greatest mean \nwet and dry implant weight (± SEM) was observed on day 14. This group was used \nas reference when comparing the treatments. In the pain group there was a \nsignificant reduction of the abdominal contortions, in the inflammation there was a \nsignificant reduction of the C reactive protein and in the oxidative stress group there \nwas a reduction of TBARS and a significant increase of the glutathione. Conclusion: \nAnimals ingesting omega-6/3 and omega-9/6 displayed lower implant weight and less \npain, inflammation and oxidative stress, but fertility was not improved. \nKey words: Endometriosis. Dietary Supplements. Fertility. Pain. Oxidative stress. \nFatty Acids, Omega-3. Inflammation.