Reducing effects of particle adsorption to the air-water interface in cryoEM
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Using the Spotiton robot and nanowire grids, this study reduces particle adsorption to the air-water interface in cryoEM by decreasing liquid film dwell time, improving data quality for biological samples.
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Abstract
Most protein particles prepared in vitreous ice for single particle cryo-electron microscopy are adsorbed to air-water or substrate-water interfaces, potentially causing particles to adopt preferred orientations. Using the Spotiton robot and nanowire grids, we can significantly reduce air-water interface issues by decreasing the dwell time of particles in thin liquid films. We demonstrate this by using single particle cryoEM and cryoET on three biological samples.
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- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00