Physical Intervention Strategy for Postharvest Shiitake Mushrooms Quality Improvement: Partial Water Loss-Induced Delay of Spore Discharge via Energy Metabolism Maintenance

Physical Intervention Strategy for Postharvest Shiitake Mushrooms Quality Improvement: Partial Water Loss-Induced Delay of Spore Discharge via Energy Metabolism Maintenance | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Physical Intervention Strategy for Postharvest Shiitake Mushrooms Quality Improvement: Partial Water Loss-Induced Delay of Spore Discharge via Energy Metabolism Maintenance Yunting Li, Yafei Wang, Zhenshan Hou, Guanlin Qian, Miao Yan, and 6 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8047947/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 13 You are reading this latest preprint version Abstract Spore discharge of postharvest shiitake mushrooms represents a critical turning point in quality deterioration, necessitating the development of effective intervention strategies to delay this process. The results demonstrated that 4% water loss (WL) treatment significantly inhibited spore discharge intensity ( P < 0.05) and reduced pileus opening indices. Partial WL (PWL) treatment delayed central carbon metabolism, resulting in higher carbohydrate retention. Notably, TCA cycle metabolites and energy-related enzyme activities, especially H + -ATPase and Ca 2+ -ATPase, remained elevated compared to the non-treated group, collectively contributing to improved energy status maintenance in treated mushrooms. Ultrastructural analysis revealed that postharvest PWL treatment exhibited immediate effects, reducing sterigmata mechanical support during early storage. These findings suggest that PWL treatment effectively inhibits spore discharge intensity and appearance quality deterioration through respiratory metabolism delay and energy metabolism enhancement. This article uncovers underlying mechanism by which PWL treatment extends the shelf life of shiitake mushrooms through its impact on spore discharge, providing new ideas for developing more easily implemented preservation technologies. As an energy- and cost- efficient method, PWL treatment has great potential for application in the food industry. Shiitake mushroom partial water loss treatment spore discharge intensity central carbon metabolism energy metabolism ultrastructure Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 27 Nov, 2025 Reviews received at journal 26 Nov, 2025 Reviews received at journal 23 Nov, 2025 Reviewers agreed at journal 18 Nov, 2025 Reviews received at journal 16 Nov, 2025 Reviews received at journal 16 Nov, 2025 Reviewers agreed at journal 13 Nov, 2025 Reviewers agreed at journal 12 Nov, 2025 Reviewers agreed at journal 12 Nov, 2025 Reviewers invited by journal 12 Nov, 2025 Editor assigned by journal 10 Nov, 2025 Submission checks completed at journal 09 Nov, 2025 First submitted to journal 06 Nov, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8047947","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":549359697,"identity":"11ef7783-87dc-4e29-9ed2-d12f90d4fbb5","order_by":0,"name":"Yunting Li","email":"","orcid":"","institution":"Shenyang Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Yunting","middleName":"","lastName":"Li","suffix":""},{"id":549359698,"identity":"7a3bf370-def5-4b07-b1a4-b3d4c6267243","order_by":1,"name":"Yafei Wang","email":"","orcid":"","institution":"Shenyang Agricultural 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The results demonstrated that 4% water loss (WL) treatment significantly inhibited spore discharge intensity (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05) and reduced pileus opening indices. Partial WL (PWL) treatment delayed central carbon metabolism, resulting in higher carbohydrate retention. Notably, TCA cycle metabolites and energy-related enzyme activities, especially H\u003csup\u003e+\u003c/sup\u003e-ATPase and Ca\u003csup\u003e2+\u003c/sup\u003e-ATPase, remained elevated compared to the non-treated group, collectively contributing to improved energy status maintenance in treated mushrooms. Ultrastructural analysis revealed that postharvest PWL treatment exhibited immediate effects, reducing sterigmata mechanical support during early storage. These findings suggest that PWL treatment effectively inhibits spore discharge intensity and appearance quality deterioration through respiratory metabolism delay and energy metabolism enhancement. This article uncovers underlying mechanism by which PWL treatment extends the shelf life of shiitake mushrooms through its impact on spore discharge, providing new ideas for developing more easily implemented preservation technologies. 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