Research on bacterial flora and antibiotic sensitivity of diabetic foot wound treated with antibiotic bone cement

Research on bacterial flora and antibiotic sensitivity of diabetic foot wound treated with antibiotic bone cement | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Research on bacterial flora and antibiotic sensitivity of diabetic foot wound treated with antibiotic bone cement Xingyu Sun, Maochun Wu, Yi Zhang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6574752/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Diabetic foot is one of the serious complications of diabetes mellitus, and diabetic foot wound infection is often fatal to patients. As a relatively new method, antibiotic bone cement treatment of diabetic foot is beneficial to many patients with diabetic foot. The aim of this study is to analyze the composition of diabetic foot microbiota and to screen antibiotics with better efficacy to add to bone cement for the treatment of foot infection wounds. Methods We collected the exudates from diabetic foot wounds of patients for bacterial culture and drug sensitivity test, and classified and analyzed the composition of bacterial flora. For drug susceptibility test results, we first screened the antibiotics by using the Boruta algorithm prediction model and then performed sensitivity analysis based on age and gender factors. Results According to this study, age was a significant factor in the cumulative sensitivity of bacteria to antibiotics added with bone cement. In the bacterial flora of diabetic foot wounds, positive bacteria accounted for a relatively high proportion, and the main species was Staphylococcus aureus. Based on the analysis, the better choices of antibiotics were Gentamicin(GEN) and Tobramycin(TOB) for negative bacteria and Moxifloxacin(MFX), Ampicillin(AMP) and Quinupristin-dalfopristin(QDA) for positive bacteria. Conclusions In the bacterial flora of diabetic foot wounds, positive bacteria accounted for a relatively high proportion, and the main species was Staphylococcus aureus. Based on our findings, it is advised that the diabetic population who requires bone cement antibiotic treatment be treated according to age, with GEN and TOB being recommended for negative bacteria and MFX, AMP, and QDA for positive bacteria. Bone cement antibiotic sensitivity analysis feature selection Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6574752","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":452627719,"identity":"c06f6ea2-cca2-47ae-ac3f-bfc48c332a89","order_by":0,"name":"Xingyu Sun","email":"","orcid":"","institution":"Jining University","correspondingAuthor":false,"prefix":"","firstName":"Xingyu","middleName":"","lastName":"Sun","suffix":""},{"id":452627720,"identity":"f191de10-bda7-45d2-8569-a626ebd44fd2","order_by":1,"name":"Maochun Wu","email":"","orcid":"","institution":"Jining polytechnic","correspondingAuthor":false,"prefix":"","firstName":"Maochun","middleName":"","lastName":"Wu","suffix":""},{"id":452627721,"identity":"e7c83592-2148-4e56-a7a5-a031e9a58b28","order_by":2,"name":"Yi Zhang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA9ElEQVRIiWNgGAWjYDACCQgpJ8/MfODAhwogg1gtxobtbYkHZ5yxMDZsIE4LQ2LDmTPGh3nbKhIZDhDQIT+7+dnDr20WxowzEgwO886TSGBsYH746AYeLYxzjpkby7ZJyLFLJCQcnLtNIo+dgc3YOAePFmaJBDNpyTYJkC0HDrzdJlHM2MDDJo1PC5tE+jeQlsSGG4kNB3jnABkHCGjhkcgxk/wI0nLmMMNB3gYitEhI5JRJM5wDBzLDwRnHgIxmAn6Rn5G+TfJHWR0wKvk/f/hQA2SwNz98jE8LCDDzsqFwCSgHAcYff4hQNQpGwSgYBSMXAAAt2k3IfsIGdAAAAABJRU5ErkJggg==","orcid":"","institution":"Zibo Central Hospital","correspondingAuthor":true,"prefix":"","firstName":"Yi","middleName":"","lastName":"Zhang","suffix":""}],"badges":[],"createdAt":"2025-05-02 01:23:15","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6574752/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6574752/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":84774367,"identity":"e2dce77a-280e-44c5-b8b0-a4cca67e76bb","added_by":"auto","created_at":"2025-06-17 08:40:07","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1267902,"visible":true,"origin":"","legend":"","description":"","filename":"TypeBMC2V.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6574752/v1_covered_4c07e0ab-e288-449d-80ed-de7b2ac2d680.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Research on bacterial flora and antibiotic sensitivity of diabetic foot wound treated with antibiotic bone cement","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Bone cement, antibiotic sensitivity analysis, feature selection","lastPublishedDoi":"10.21203/rs.3.rs-6574752/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6574752/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eDiabetic foot is one of the serious complications of diabetes mellitus, and diabetic foot wound infection is often fatal to patients. As a relatively new method, antibiotic bone cement treatment of diabetic foot is beneficial to many patients with diabetic foot. The aim of this study is to analyze the composition of diabetic foot microbiota and to screen antibiotics with better efficacy to add to bone cement for the treatment of foot infection wounds.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eWe collected the exudates from diabetic foot wounds of patients for bacterial culture and drug sensitivity test, and classified and analyzed the composition of bacterial flora. For drug susceptibility test results, we first screened the antibiotics by using the Boruta algorithm prediction model and then performed sensitivity analysis based on age and gender factors.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eAccording to this study, age was a significant factor in the cumulative sensitivity of bacteria to antibiotics added with bone cement. In the bacterial flora of diabetic foot wounds, positive bacteria accounted for a relatively high proportion, and the main species was Staphylococcus aureus. Based on the analysis, the better choices of antibiotics were Gentamicin(GEN) and Tobramycin(TOB) for negative bacteria and Moxifloxacin(MFX), Ampicillin(AMP) and Quinupristin-dalfopristin(QDA) for positive bacteria.\u003c/p\u003e\u003ch2\u003eConclusions\u003c/h2\u003e \u003cp\u003eIn the bacterial flora of diabetic foot wounds, positive bacteria accounted for a relatively high proportion, and the main species was Staphylococcus aureus. Based on our findings, it is advised that the diabetic population who requires bone cement antibiotic treatment be treated according to age, with GEN and TOB being recommended for negative bacteria and MFX, AMP, and QDA for positive bacteria.\u003c/p\u003e","manuscriptTitle":"Research on bacterial flora and antibiotic sensitivity of diabetic foot wound treated with antibiotic bone cement","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-05-08 18:47:49","doi":"10.21203/rs.3.rs-6574752/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b6cb5cab-63d8-48a5-87b7-c930cc373913","owner":[],"postedDate":"May 8th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-06-17T08:39:49+00:00","versionOfRecord":[],"versionCreatedAt":"2025-05-08 18:47:49","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6574752","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6574752","identity":"rs-6574752","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}