TX2P: A Proteogenomic Tool for Comprehensive Transcript Analysis

ABSTRACT Long-read RNA sequencing has expanded our understanding of the transcriptome, revealing unannotated transcripts. However, interpreting their functional relevance remains challenging. To address this, we developed TX2P, a user-friendly tool that integrates transcriptomic and proteomic data to link RNA discoveries with protein function. Applied to epilepsy-associated genes, TX2P identified novel protein-coding transcripts, with peptide evidence supporting 17.2% transcripts and 18.0% of unique open reading frames. By seamlessly integrating transcriptomic and proteomic insights, TX2P facilitates transcriptome interpretation. Competing Interest Statement The authors have declared no competing interest. Funder Information Declared Aligning Science Across Parkinson's, https://ror.org/03zj4c476, ASAP-000509, ASAP-000478 BrightFocus Foundation, A2021009F Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.