PPMs, PGPB and Microalgae as Three Distinct Biofertilizers on Lettuce Health

PPMs, PGPB and Microalgae as Three Distinct Biofertilizers on Lettuce Health | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article PPMs, PGPB and Microalgae as Three Distinct Biofertilizers on Lettuce Health Nurgül KITIR ŞEN This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4412276/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The utilization of biofertilizers has emerged as a promising approach in modern agriculture to enhance crop productivity and quality while promoting environmental sustainability. In this study, we investigated the efficacy of three distinct biofertilizers, namely Potential Plant Probiotic Microorganisms (PPMs), Plant Growth-Promoting Bacteria (PGPB), and Microalgae, on the health on lettuce ( Lactuca sativa L. var. Crispa ). The experiment was conducted in a controlled environment of a high tunnel greenhouse at Gebze Technical University. The study encompassed a comprehensive analysis of various growth parameters, including plant height, stem diameter, leaf count, plant weight, root length, and chlorophyll content related with plant health. Our findings demonstrate that the application of PPMs, PGPB, and Microalgae biofertilizers significantly improved lettuce yield and quality compared to traditional fertilization methods. Moreover, these biofertilizers positively influenced soil and plant health, contributing to enhanced microbial diversity and soil structure. Additionally, our study highlights the environmental and economic benefits associated with biofertilizer usage, paving the way for sustainable agricultural practices. Overall, this research underscores the potential of PPMs, PGPB, and Microalgae biofertilizers as effective alternatives in lettuce cultivation, offering promising prospects for future agricultural innovations and practices. Lettuce Health Biofertilizer Plant Probiotic Microorganisms (PPMs) Plant Growth-Promoting Bacteria (PGPB) Microalgae Yield Quality Figures Figure 1 Figure 2 Highlights Effectiveness of Different Biofertilizers : The study compares the efficacy of various biofertilizers such as Potential Plant Probiotic Microorganisms (PPMs), Plant Growth-Promoting Bacteria (PGPB), and Microalgae on lettuce growth and development. It determines the impact of these biofertilizers on lettuce yield and quality. Yield Enhancement : Research indicates that PPMs, PGPB, and Microalgae biofertilizers are effective in increasing lettuce yield. Their usage leads to higher yields compared to traditional fertilization methods. Quality Improvement : The study demonstrates that biofertilizers contribute positively to improving the quality of lettuce. Observations show that leaves are healthier, juicier, and more flavorful. Contribution to Soil Health : PPMs, PGPB, and Microalgae biofertilizers are found to have positive contributions to soil health. They increase soil microbial diversity and enhance soil structure. Environmental and Economic Benefits : The use of biofertilizers is highlighted as environmentally sustainable, reducing chemical fertilizer usage and minimizing environmental impacts. Additionally, biofertilizers are economically beneficial for farmers. Importance of Application Methods and Dosages : The study emphasizes that the effectiveness of biofertilizers depends on application methods and dosages. It underscores the importance of determining suitable application methods and dosages to achieve optimal results. INTRODUCTION Lettuce ( Lactuca sativa L.) stands as the sole member of the genus Lactuca cultivated on a global scale for commercial purposes. Primarily valued for its leafy foliage, lettuce has surged in popularity and is now a commonly consumed vegetable worldwide. Lettuce, including head lettuce, romaine lettuce, and curly lettuce, is one of the most consumed leafy vegetables (Shi et al., 2022 ). The primary concern associated with the traditional use of fertilizers in leafy vegetables, which are consumed in their leaf form, is the potential accumulation of nitrates, which can increase the risk of cancer (Bian et al., 2020 ). The choice of fertilizer plays a crucial role in determining nitrogen availability and lettuce yield (Urra, Alkorta, Mijangos, & Garbisu, 2020). Increasing yield and quality of lettuce has potential contribution with Nitrogen content of fertilizers and it can be sourced from various sources, including inorganic fertilizers like urea, as well as organic alternatives such as blood meal, feather meal, compost, and farm manure (Smith & Hadley, 1989). While some growers may prefer inorganic nitrogen fertilizers due to their ease of application and faster nitrogen release compared to organic options. In the literature, there are reported studies relating to increase quality with many kind of these fertilizer sources that includes different Nitrogen sources which have crucial points. Soil-plant interactions are a key factor in the physiological activities of plants, and resistance to soil properties and climate change is also crucial as Nitrogen sources. Studies have shown that PGPR or microbial fertilizers can affect the growth conditions of specific microorganisms living in the soil, thus altering plant growth and development related with usage of atmosphere Nitrogen as a different N source. Beneficial microorganisms that can be applied as inoculants to increase soil microbial diversity can be defined as Plant Probiotic Microorganisms (PPMs) (de Souza Vandenberghe et al., 2017 ). The functions of PPMs include free nitrogen fixation, suppression of soil-borne pathogens, production of simple organic molecules for plant uptake, dissolution of insoluble nutrient sources, production of polysaccharides to improve soil aggregation, etc. Additionally, it has been reported that these PPMs applications can protect plant cells against oxidative damage and increase plant resistance to saline conditions. The importance of these biotechnological products for sustainable environmental improvement and eco-innovation has also been reported in many studies (Talaat, Shawky, & Botany, 2014 ) Chemical fertilizers containing high levels of nitrogen, such as ammonium nitrate, ammonium sulfate, urea, and similar ones commonly used in traditional agriculture, cannot be used in organic farming (Fulya, SAĞLAM, & Journal, 2018 ). Therefore, providing the nitrogen needed by plants with alternative and organic solutions has become important. Animal-derived large and small livestock manures, worm castings, and bat guano are all viable options for providing nitrogen in organic farming practices (ABD RAHMAN, TINGGA, BUKHORI, ABDULLAH, & Technology, 2023). However, when these fertilizers are considered only as a nitrogen source, the necessity of annual application, difficulties in procurement, and application challenges arise (Tyagi, Ahmad, Malik, & Technology, 2022). Moreover, bacterial solutions, i.e., microorganism applications can be a continuous solution as a self-renewing nitrogen source with the condition of establishing a certain population in the soil (Akimbekov et al., 2022 ). Therefore, it is necessary to introduce new products to the agricultural sector in this regard. This research is designed to investigate the effects of microbial products containing Bacillus subtilis , Bacillus megaterium , Bacillus amyloliquefaciens (Type 1: PGPR), Lactobacillus lactis, Lactobacillus cremoris, Lactobacillus acidophilus, Lactobacillus plantarum, Rhodopseudomonas palustris, Saccharomyces cerevisiae (Type 2: PPMs), Chlorella vulgaris (Type : Microalgae), on lettuce cultivation under greenhouse conditions, aiming to determine their effects on plant growth, yield, and some quality criteria. MATERIAL and METHODS 1.1. Cultivar of Cultivated Plant In the research conducted within the greenhouse located at the Research and Application Field of Gebze Technical University, the cultivar Festival curly lettuce ( Lactuca sativa L. var. Crispa) was used as the plant material. It is suitable for cultivation in every season. The plant has a robust structure with open green leaves, presenting an attractive appearance with its glossy foliage. Due to its numerous leaves, it has a large appearance, and its leaf edges are extremely curly. It has resistance to bolting and does not break during harvesting and washing due to its flexible leaf structure. It maintains leaf quality for a long time, thus having a long shelf life. It has high tolerance to tip burn. It is tasty with its juicy and crisp leaf structure. Suitable for greenhouse, low tunnel, and field production. The number of plants to be planted per hectare ranges from 5,500 to 7,000. Disease Resistance: BI: 1–20, 22–24 (HR) LMV: 2 (IR). 1.2. Trial Location and Characteristics This study was conducted in the High Tunnel Greenhouse located within the Gebze Technical University Campus from December (2022) to March (2023). Gebze Technical University is located within the boundaries of the Gebze district. The trial coordinates were taken as Gebze Technical University - GPS coordinates 40° 48' 27.1764" and 29° 21' 50.8536". In the study, 3–4 true leaf seedlings obtained from a private seedling company were arranged in the soil greenhouse with a drip irrigation system, with at least 10 plants per row and row spacing of 50 cm x 30 cm. 1.3. Soil Characteristics Some physical and chemical properties of the soil in the region where the study was conducted are given in Table 1 . Soil samples were taken from the greenhouse and sent to a special soil analysis laboratory. According to the analysis data, the soil structure is clayey loam, and the amount of organic matter and plant-available nitrogen, phosphorus, potassium, and trace elements were considered sufficient (Table 1 ). For crop growth, combined applications with farmer control and farmer practices, 500 g/ha − 1 of NPK fertilizer (Quatro 18.18.18) were applied three times (monthly) via drip irrigation. Additionally, 2 L/ha − 1 of liquid sulfur was applied to balance the calcareous structure. Lettuce plants can complete their growth period most healthily in sandy soils. When the soil texture was examined, it was found to have a clayey loam structure, which is suitable for lettuce cultivation. 1.4. Soil Analysis Soil samples were taken (0–30 cm, 30 subsamples) to determine some soil properties. After the soil samples have been taken, exchangeable cations was determined using ammonium acetate (Thomas et al., 1982 ). and cation exchange capacities (CEC) were determined using sodium acetate—ammonium acetate (Sumner & Miller, 1996). Total N was determined by the Kjeldahl method (Bremner, 1996), plant-available P was determined by using the sodium bicarbonate method (Olsen, 1954 ). Electrical conductivity (EC) was measured in saturation extracts (Rhoades, 1996). Calcium carbonate and soil pH were determined as a method by (McLean & properties, 1983). Soil organic matter was determined using the Smith-Weldon method (Nelson & Sommers, 1996). Available Fe, Mn, Zn, and Cu in the soils were determined by DTPA methods (Lindsay & Norvell, 1978 ). Available B was analyzed using the azomethine-H (Wolf & Analysis, 1974).These soil characterization data are presented in Table 1 . Table 1 . Some Physical and Chemical Properties of the Trial Soil (mean ± standard deviation, n = 16) 1.5. Experimental Design and Arrangement The research was conducted in a high tunnel greenhouse as a pot trial, with a randomized complete block design consisting of 8 factors (Control, Positive Control, Microalgae, Microalgae + Positive Control, PPMs, PPMs + Positive Control, PGPB, PGPB + Positive Control), 5 replications (drip line order), and 7 plants per replication (per row). The obtained data were analyzed using IBM SPSS 23 software package, and statistical comparisons between the means were performed using one-way ANOVA and Duncan test. The statistical significance level was set at p ≤ 0.05. 1.6. The Biofertilizers and Their Properties In the trial three different biofertilizer formulations were used. The first group of biofertilizer has been formulated with Microalgae: Chlorella vulgaris (1x10 6 cfu/ml) and the second group of formulated biofertilizer is composed by PPMs: Lactobacillus lactis, Lactobacillus cremoris, Lactobacillus acidophilus, Lactobacillus plantarum, Rhodopseudomonas palustris, Saccharomyces cerevisiae (7x10 6 cfu/ml) and the third formulation is in PGPB group including Bacillus subtilis, Bacillus megaterium and Bacillus amyloliquefaciens (1x10 8 cfu/ml). 1.7. Type of Equipment Used To ensure uniform distribution of fertilizers and no differences between applications, equal amounts of irrigation were applied every other day to match the field capacity of the soil in the greenhouse. Since no damage or disease was encountered during the cultivation period under tunnel greenhouse (temperature degree) conditions, no spraying was deemed necessary. Planting of curly lettuce (Festival) seedlings was done manually. Only the same amount of water was applied to the control groups where no product was applied. The entire area was subjected to fertilization conditions under the same farmer conditions. Periodically, weeds in the greenhouse soil were cleared using cultural methods. Product application was carried out using a graduated pump sprayer and fertilizer tanks, with the product mixed with water and applied via drip irrigation system. Plant diameters were measured using a caliper, and heights were measured using a meter at harvest. Plant weights were measured as fresh weight on sensitive scales (± 0.5 g). Chlorophyll values in plants were measured using a portable chlorophyll meter device from Scout brand. Greenhouse air humidity and temperature were measured using an Htc thermometer digital hygrometer temperature and humidity alarm device. Soil pH was measured using a Motorobit brand pH meter. According to the data obtained, the average pH in the untreated groups was found to be 7.4, while in the treated groups, the average pH decreased to around 6.6. It is known that ideal pH ranges for curly lettuce cultivation fall within these values. 1.8. Application Timing and Frequency The research was conducted in a high tunnel greenhouse as a pot trial, with a randomized complete block design consisting of 8 factors. Control : No chemical or biological fertilizer application was made to the soil. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. Positive Control : In farmer control applications, 500 g/ha of NPK fertilizer (Quatro 18.18.18) was applied three times per month via drip irrigation. Liquid sulfur application was made at a rate of 2 L/ha-1 to balance the calcareous structure. Additionally, organic base fertilizer was applied at a rate of 100 kg/ha to enrich the organic matter. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. PPMs : Lettuce seedlings were planted by dipping the roots into a solution prepared by mixing 500 ml of PPMs in 100 liters of water before planting. The lettuce seedlings were irrigated with 2 liters of PPMs per decare on the first planting date, and irrigation was done every 15 days until harvest on May 5, 2023, with 1 liter of PPMs per decare. From planting to harvest, 200 ml of PPMs per 100 liters of water was sprayed on the leaves at weekly intervals. Applications were made on cool days when direct sunlight was not present. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. Positive Control + PPMs : In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted by dipping the roots into a solution prepared by mixing 500 ml of PPMs in 100 liters of water. Lettuce seedlings were irrigated with 2 liters of PPMs per decare on the first planting date, and irrigation was done every 15 days until harvest on May 5, 2023, with 1 liter of PPMs per decare. From planting to harvest, 200 ml of PPMs per 100 liters of water was sprayed on the leaves at weekly intervals. Applications were made on cool days when direct sunlight was not present. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. PGPB : In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted using drip irrigation with 3 L/da of Losepa prepared at a rate of 100 liters of water per 3 liters. Applications were made twice at 15-day intervals from the planting date to 15 days later, and then 3rd and 4th applications were made every 15 days using PGPB prepared with 3 L/da of water per 100 liters of water as spray from the leaves. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. Positive Control + PGPB : In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted using drip irrigation with 3 L/da of PGPB prepared at a rate of 100 liters of water per 3 liters. Applications were made twice at 10-day intervals from the planting date to 15 days later, and then 3rd and 4th applications were made every 15 days using Losepa prepared with 3 L/da of water per 100 liters of water as spray from the leaves. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. Microalgae : In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted using drip irrigation with 1500 cc of Microalgae prepared at a rate of 1000 liters of water per da. Applications were made twice at 10-day intervals from the planting date to 14 days later, and then 3rd, 4th, and 5th applications were made every 14 days using Microalgae prepared with 1500 cc of water per 1000 liters of water as spray from the leaves. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. Positive Control + Microalgae : In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted using drip irrigation with 1500 cc of Microalgae prepared at a rate of 1000 liters of water per da. Applications were made twice at 10-day intervals from the planting date to 14 days later, and then 3rd, 4th, and 5th applications were made every 14 days using Microalgae prepared with 1500 cc of water per 1000 liters of water as spray from the leaves. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. 1.9. Meteorological Data Meteorological data obtained from the General Directorate of Meteorology for the period from December 15, 2022, to March 5, 2023, covering the trial area, showed minimum, maximum, and average temperatures of 3.5, 13, and 8.25°C, respectively. Throughout the vegetation period, the average minimum and maximum temperatures in the greenhouse ranged between 10–15°C. Curly lettuce cultivation conforms to ideal standards 8–20°C, and no additional heating or cooling was required in the greenhouse. Greenhouse humidity values were measured between 70–75% using a humidity meter throughout the vegetation period, and ventilation was performed at certain intervals to maintain this humidity. 1.10. Planting duration Planting Date: November 15, 2022, Harvest Date: March 5, 2023, Vegetation Period: 80 days, Planting Conditions: GTU Soil Greenhouse Trial Drip Irrigation System 1.11. Measurements and Analyses The characteristics and methods examined in the study are as follows: Plant Height (cm/plant): At the completion of the trial, the region from the root collar to the growth tip was measured in centimeters (± 0.5 cm) using a meter in three randomly selected plants (per replication), Plant Diameter (cm/plant): The stem diameter was determined in millimeters (± 0.1 mm) using a digital caliper Number of Leaves Plant Weight (g), Root Length (cm/plant) (Pandey, Paul, Das, Meena, & Meena, 2017) Chlorophyll (SPAD): Chlorophyll levels in plants were measured using a portable chlorophyll meter device from Scout brand (Yin et al., 2023 ). 2. STATISTICAL ANALYSIS The data was analyzed by One-Way ANOVA with SPSS software (version 25.0). The Duncan test was used to determine meaningful differences between the means (p < 0.05 and p < 0.01). PCA (Principal Component Analysis) was analysed on Minitab 17, Path Analysis was made with R studio program and Decision Tree Analysis was made with with SPSS software (version 25.0). RESULTS AND DISCUSSION Soil samples were analyzed with the following values: pH 7.3±0.17, EC 0.02±0.001 (dS/cm), calcium carbonate 1,7±0.15%, OM 1,65±0.14% (Table 1). Table 1. Some Physical and Chemical Properties of the Trial Soil The variance analysis and application averages showing the interaction difference of fertilizer applications are given in Tables 2 and 3. According to the variance analysis, the effect on parameters such as plant weight, height, diameter, root length, chlorophyll content, and leaf count was found to be significant (p < 0.01), with the C.V% values generally ranging between 20 and 30 when considered. This indicates that application errors or deviations are within acceptable limits Upon examining the application averages (Table 3), it was observed that fertilizers exhibit a significant positive difference compared to the control group. Especially, applications where the positive control is combined with PGPB and PPMs have been found to yield better results compared to other groups. This indicates the active role of bacteria in the PGPB and PPMs groups in making the nutrients in the fertilizer beneficial to the plant. Similar results were also obtained with combined applications involving microalgae-containing fertilizers. In conclusion, applying these treatments along with chemical fertilizers in lettuce plants has improved plant photosynthetic efficiency, resulting in better plant yield and quality. Table 2. Analysis of Variance (ANOVA) Table 3. Application Averages on Plant Growth Parameters Path analysis, presented in Table 4, examines the effects of factors considered as dependent variables on plant weight. Path analysis is concerned with estimating the magnitude of connections between variables and providing information about the underlying causal processes (Simons, Conger, & Whitbeck, 1988). Upon examining Table 4, significant correlations at the 5% - 1% level were found among all the examined factors, except for the correlations between chlorophyll/plant weight and root length/plant weight. When discussing the correlations between plant weight and other factors, it is observed that the highest correlation with plant weight is leaf count. According to path analysis, while the direct effect of plant height on plant weight is 11.3%, the highest indirect effect is between leaf count (35.946%) and root length (42.95%) with a total indirect effect of 78.896%. For plant diameter, the direct effect on plant weight is 5.63%, while the highest indirect effects are through leaf count (43.78%) and root length (38.035%) with a total indirect effect of 87.415%. The direct effect of leaf count on plant weight is 57.61%, while the highest indirect effect is through root length (0.85%) with a total indirect effect of 39.85%. The effect of chlorophyll content on plant weight is 39.93%, with the highest indirect effect through leaf count (40.639%) and root length (39.235%). The direct effect of root length on plant weight is 44.552%, with the highest indirect effect through chlorophyll content (39.235%). Path analysis shows that the marketable value of the plant weight is mainly shaped under the influence of leaf count and root length. The vegetative weight, which expresses plant yield, is the result of a complex chain of activities involving all these factors. However, some factors are more significant than others, as evidenced by their importance relative to other factors (Saleh & environment, 2012). Accordingly, in our study, leaf count and root length have been shown to be the most influential factors affecting plant weight. Leaves are crucial organs containing metabolic activities involving both catabolic and anabolic processes, and they play a key role in photosynthetic activities and dry matter production. Similarly, roots are the most important part of the plant as they transport water and nutrients to the plant. As root length increases, the plant's attachment to the soil and uptake of water and nutrients also increase (Lai, Wang, Peng, & Chen, 2011). The application of PGPB, PPMs, and microalgae in conjunction with chemical fertilizers in our study positively affected leaf count and root length, the most influential factors affecting plant weight, thus, this combination can be safely used to increase plant yield. Table 4. Path Analysis Principal component analysis can be summarized as a graphical method that compresses data obtained through classification, making it more understandable by reducing dimensions(ROTARU, POP, VATCĂ, CIOBAN, & Horticulture, 2012).The aim of this technique is to examine multidimensional data and express them with fewer variables based on fundamental characteristics (Eriksson, Byrne, Johansson, Trygg, & Vikström, 2013). Principal component analysis is widely used in agriculture to reliably demonstrate the relationship and performance of applied factors and examined elements (Granato et al., 2018). The factors examined in our study and their relationship with each other are shown in Figure 1. As seen in Figure 1, the examined elements and applied factors have formed three different groups. Group: Microalgae, Microalgae + positive control, PGPB + Positive control, leaf count, plant height, plant diameter, chlorophyll, and root weight. Group: Plant weight, PPMs, and PPMs + Positive Control. Group: Control, Positive Control, and PGPB. Although the applications PPMs and PPMs + Positive Control in Group 2 were identified as the most influential applications on plant weight, they only manifest themselves when the plant is healthy and when the maximum benefit from fertilizer is desired. In other words, the effectiveness of plant probiotics can be more possible when the plant is well-developed and healthy. In the first group mentioned (Microalgae, Microalgae + Positive Control, PGPB + Positive Control), the factors affecting plant weight, such as root length, chlorophyll count, plant diameter, plant height, and leaf count, are included in the same group, and this group has been identified as the most effective group on plant growth and yield. In other words, the most effective applications on plant weight are Microalgae, Microalgae + Positive Control, and PGPB + Positive Control. The individual applications of Positive Control and PGPB did not show the expected effect on plant growth. In conclusion, Microalgae, Microalgae + Positive Control, PGPB + Positive Control, PPMs + Positive Control, and PPMs can be recommended as the most effective groups for plant performance. The effectiveness of chemical fertilizers in use is known to be around 5-10% in the soil. Figure 1. PCA Analysis Decision tree analysis is a method commonly used in social sciences but can also be applied in agricultural and natural sciences. The primary purpose of this analysis is to determine which main factors have the most influence on the dependent variable and how other factors are shaped by the scarcity or abundance of this main factor, ultimately revealing the numeric value of the dependent variable (Rajeswari, Suthendran, & Agriculture, 2019). The tree diagram illustrating the analysis of data obtained from the research is presented in Figure 2. In the analysis, it was determined that the main factor affecting the dependent variable, which is plant weight, is the leaf count. As the leaf count increases, the plant weight also increases. When the leaf count is less than 45, the plant weight is determined to be 166.743 grams, while it can reach an estimated plant weight of 320.4 grams when the leaf count exceeds 45. When the leaf count is less than 45, root weight comes into play as the main factor. As root length increases, so does plant weight. When the root length is between 3-4 cm, the estimated plant weight is 116.00 grams. Similarly, when the root length is between 3-4 cm, the main factor affecting plant weight is plant diameter. When the plant diameter is less than 16 cm, the estimated plant weight is 105.250 grams, whereas it is predicted to be 137.5 grams when the plant diameter exceeds 16 cm. In cases where the root length is 5,6,8 and 10-13 cm, chlorophyll amount emerges as the main factor. When the chlorophyll amount decreases below 163 SPAD, the estimated plant weight is 143.843 grams, while it is 209 grams when the chlorophyll amount is between 163-177, and 147.22 grams when the chlorophyll amount exceeds 177 SPAD. It is determined that the SPAD range of 163-177 provides the highest plant weight. When the root length is between 7 and 9 cm, the leaf count emerges as the main factor, and as the leaf count increases up to a certain point, the estimated plant weight also increases. Based on the variation in leaf count, the estimated plant weight ranges from 151 grams when the leaf count is less than 35 to 265 grams when it exceeds 43. As a result, the main factor affecting plant weight is the leaf count, followed by root length. Additionally, plant diameter and chlorophyll are identified as significant factors depending on the increase or decrease of the two main factors. The conditions for achieving the highest plant yield are determined to be a leaf count between 40-43, root length between 7-9 cm, a relatively large plant diameter, and chlorophyll levels between 163-177 SPAD values. Figure 2. Decision Tree Analysis There are many reviews offering an overview of the vast information about microorganisms, and specially bacteria, that are plant growth promoters and biocontrol agents, and also reviewing the mechanisms associated with these particular functions, as well as more publications showing and/or reviewing the benefits to plants, such as the enhancement of bioactive compounds in the edible parts, among others (Gouda & Saranga, 2018) (Etesami, Maheshwari, & safety, 2018; Menendez & Garcia-Fraile, 2017) (Chiboub, Jebara, Abid, & Jebara, 2020; Santoyo, Moreno-Hagelsieb, del Carmen Orozco-Mosqueda, & Glick, 2016). Briefly, PPBs have features involved in the (i) facilitation of nutrient acquisition, (ii) production of phytohormones and modulation of their levels, (iii) tolerance to either abiotic or biotic stresses, (iv) production of siderophores and other metabolites, and (v) induction of disease resistance, among other properties not listed here. The use of plant probiotic bacteria as inoculants has the potential to increase crop yield without the overapplication of chemical fertilizers, pesticides, and fungicides, and consequently to reduce the environmental impact in agriculture and maximize the production of heathier and safer foods. PPB establish synergies and act complementarily when forming a consortium, providing beneficial effects to crops. Although a high number of these associations are well-documented and most of them show positive results, there is still a lack of knowledge on how these consortia behave and interact with plants, environments, and other the microbiome. The microbes that live in and on the plant microbiome are critical for plant health and exert their influence by facilitating the nutrient acquisition, regulating plant hormone levels, and helping to withstand pathogen attack. Plants are meta-organisms that are associated with complex microbiomes. The majority of the microorgansims including epiphytes and endophytes generally play a significant role in providing essential nutrients to the plants where they live. CONCLUSION As a result of the study, it has been determined that biofertilizer applications significantly enhance plant health and development compared to the control groups. Especially, the efficiency of productivity, particularly with the application of PGPB, followed by Microalgae and PPMs, in combination with chemical fertilizers, has positively impacted the productivity and quality parameters examined in lettuce cultivation. The efficacy of chemical fertilizers is increased. The use of biological fertilizers in conjunction with chemical fertilizers enhances leaf count and root length, leading to an increase in chlorophyll content, plant diameter, and plant height. Therefore, with the use of combined fertilizers, an increase in both leaf count and root length results in higher plant yield per unit area. The highest plant yield is achieved when the leaf count is above 40, the root length is between 7–9 cm, the plant diameter exceeds 16 cm, and the chlorophyll content is approximately between 160–180 SPAD. Biological fertilizers exhibit much better performance in terms of crop yield and quality when used in combination with chemical fertilizers rather than being used alone. Probiotics are not only important for human health but also crucial for plant and soil health. We may conclude that the use of these beneficial bacteria combined in consortia is very important for improving lettuce yields and performance. However, the overall ecological impact is still not well-known and must be addressed in future studies if the successful and sustainable application of bacterial consortia is to be pursued for a better, greener, and more profitable agriculture. Abbreviations Promoting Bacteria (PGPB), Plant Probiotic Microorganisms (PPMs) Declarations Availability of data and materials : Not applicable. Funding: This research was not funded Competing Interests: We know of no conflicts of interest associated with this publication, and there has been no significant financial support for this work that could have influenced its outcome. As Corresponding Author, I confirm that the manuscript has been read and approved for submission by all the named authors Ethics approval : Not applicable. Consent to participate : Not applicable. Consent for publication : Not applicable Author Contribution All the studies were done by Assoc. Prof. Nurgül Kıtır Şen for this manuscript. Acknowledgement Thanks to Binafert Biyoteknoloji Tarım ve Yazılım A.Ş. for supporting this study. References ABD RAHMAN, S.-S. N., TINGGA, R. C. T., BUKHORI, M. F. M., ABDULLAH, S. M. A. A. J. B. J. o. R. S., & Technology. (2023). A brief review of the nutritive value and chemical components of bat guano and its potential use as a natural fertiliser in agriculture. 13 (1), 22–31. Akimbekov, N. S., Digel, I., Tastambek, K. T., Marat, A. K., Turaliyeva, M. A., & Kaiyrmanova, G. K. J. B. (2022). Biotechnology of microorganisms from coal environments: from environmental remediation to energy production. 11 (9), 1306. Bian, Z., Wang, Y., Zhang, X., Li, T., Grundy, S., Yang, Q., & Cheng, R. J. F. (2020). A review of environment effects on nitrate accumulation in leafy vegetables grown in controlled environments. 9 (6), 732. Bremner, J. M. J. M. o. s. a. P. C. m. (1996). Nitrogen-total. 5 , 1085–1121. Chiboub, M., Jebara, S. H., Abid, G., & Jebara, M. J. J. o. p. g. r. (2020). Co-inoculation effects of Rhizobium sullae and Pseudomonas sp. on growth, antioxidant status, and expression pattern of genes associated with heavy metal tolerance and accumulation of cadmium in Sulla coronaria. 39 , 216–228. de Souza Vandenberghe, L. P., Garcia, L. M. B., Rodrigues, C., Camara, M. C., de Melo Pereira, G. V., de Oliveira, J., & Soccol, C. R. J. A. m. (2017). Potential applications of plant probiotic microorganisms in agriculture and forestry. 3 (3), 629. Eriksson, L., Byrne, T., Johansson, E., Trygg, J., & Vikström, C. (2013). Multi-and megavariate data analysis basic principles and applications (Vol. 1): Umetrics Academy. Etesami, H., Maheshwari, D. K. J. E., & safety, e. (2018). Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects. 156 , 225–246. Fulya, T., SAĞLAM, C. J. I. A. R., & Journal, E. (2018). A different method of using nitrogen in agriculture; Anhydrous ammonia. 2 (1), 43–47. Gouda, S. K., & Saranga, H. J. I. J. o. P. R. (2018). Sustainable supply chains for supply chain sustainability: impact of sustainability efforts on supply chain risk. 56 (17), 5820–5835. Granato, D., Santos, J. S., Escher, G. B., Ferreira, B. L., Maggio, R. M. J. T. i. F. S., & Technology. (2018). Use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) for multivariate association between bioactive compounds and functional properties in foods: A critical perspective. 72 , 83–90. Lai, W.-L., Wang, S.-Q., Peng, C.-L., & Chen, Z.-H. J. W. r. (2011). Root features related to plant growth and nutrient removal of 35 wetland plants. 45 (13), 3941–3950. Lindsay, W. L., & Norvell, W. J. S. s. s. o. A. j. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. 42 (3), 421–428. McLean, E. J. M. o. s. a. P. C., & properties, m. (1983). Soil pH and lime requirement. 9 , 199–224. Menendez, E., & Garcia-Fraile, P. J. A. m. (2017). Plant probiotic bacteria: solutions to feed the world. 3 (3), 502. Nelson, D. W., & Sommers, L. E. J. M. o. s. a. P. C. m. (1996). Total carbon, organic carbon, and organic matter. 5 , 961–1010. Olsen, S. R. (1954). Estimation of available phosphorus in soils by extraction with sodium bicarbonate : US Department of Agriculture. Pandey, R., Paul, V., Das, M., Meena, M., & Meena, R. C. J. M. o. I. S. T. P. o. P. T. t. A. t. I. o. C. C. o. C. P. (2017). Plant growth analysis. 103–107. Rajeswari, S., Suthendran, K. J. C., & Agriculture, E. i. (2019). C5. 0: Advanced Decision Tree (ADT) classification model for agricultural data analysis on cloud. 156 , 530–539. Rhoades, J. J. M. o. s. a. P. C. m. (1996). Salinity: Electrical conductivity and total dissolved solids. 5 , 417–435. ROTARU, A. S., POP, I. D., VATCĂ, A., CIOBAN, A. J. B. o. t. U. o. A. S., & Horticulture, V. M. C.-N. (2012). Usefulness of principal components analysis in agriculture. 69 (2). Saleh, B. J. S., & environment. (2012). Salt stress alters physiological indicators in cotton (Gossypium hirsutum L.). 31 (2). Santoyo, G., Moreno-Hagelsieb, G., del Carmen Orozco-Mosqueda, M., & Glick, B. R. J. M. r. (2016). Plant growth-promoting bacterial endophytes. 183 , 92–99. Shi, M., Gu, J., Wu, H., Rauf, A., Emran, T. B., Khan, Z.,.. . Al-Awthan, Y. S. J. A. (2022). Phytochemicals, nutrition, metabolism, bioavailability, and health benefits in lettuce—A comprehensive review. 11 (6), 1158. Simons, R. L., Conger, R. D., & Whitbeck, L. B. J. J. o. D. i. (1988). A multistage social learning model of the influences of family and peers upon adolescent substance abuse. 18 (3), 293–315. Sumner, M. E., & Miller, W. P. J. M. o. s. a. P. C. m. (1996). Cation exchange capacity and exchange coefficients. 5 , 1201–1229. Talaat, N. B., Shawky, B. T. J. E., & Botany, E. (2014). Protective effects of arbuscular mycorrhizal fungi on wheat (Triticum aestivum L.) plants exposed to salinity. 98 , 20–31. Thomas, E. D., Sanders, J. E., Buckner, C. D., Papayannopoulou, T., Borgna-Pignatti, C., De Stefano, P.,.. . Storb, R. J. T. L. (1982). Marrow transplantation for thalassaemia. 320 (8292), 227–229. Tyagi, J., Ahmad, S., Malik, M. J. I. J. o. E. S., & Technology. (2022). Nitrogenous fertilizers: Impact on environment sustainability, mitigation strategies, and challenges. 19 (11), 11649–11672. Urra, J., Alkorta, I., Mijangos, I., & Garbisu, C. J. J. o. e. m. (2020). Commercial and farm fermented liquid organic amendments to improve soil quality and lettuce yield. 264 , 110422. Wolf, B. J. C. i. S. S., & Analysis, P. (1974). Improvements in the azomethine-H method for the determination of boron. 5 (1), 39–44. Yin, H., Huang, W., Li, F., Yang, H., Li, Y., Hu, Y.,.. . Science, G. (2023). Multi-temporal UAV Imaging-Based Mapping of Chlorophyll Content in Potato Crop. 91 (2), 91–106. Tables Table 1. Some Physical and Chemical Properties of the Trial Soil Soil Properties Unit Means Nitrogen(N) % 1,61±0.07 *Organic Matter % 1,65±0.14 Plant available P mg/kg 4,31±0.48 Exchangeable K Cmolc/kg 5,17±0.85 Structure % 55,4±1.41 *CaCO3 % 1,70±0.15 pH (1:2 soil:water) _ 7,3±0,17 *Electric Conductivity(EC) dS/cm 0,02±0,001 Exchangeable Ca Cmolc/kg 17,5±1.41 Exchangeable Mg Cmolc/kg 2,58±0.23 Exchangeable Na Cmolc/kg 0,83±0.12 Available Fe mg/kg 3,51±0.45 Available Zn mg/kg 11.4±0.51 Available Cu mg/kg 5,37±1.46 Available Mn mg/kg 3,58±1.36 Cation Exchangeable Capacity (CEC) meq/kg 65,3±1.45 Available B mg/kg 1,33±0.14 Sand % 35,31±1.45 Clay % 33,34±1.72 Silt % 31,35 ±2.13 Table 2. Analysis of Variance (ANOVA) Plant Weight(gr) Plant Height(cm) Plant Diameter(cm) Source of Var. df MS F value MS F value MS F value Replication 4 1251.413 0.997ns 6.500 1.685ns 9.588 0.893ns Fertilizer 7 37346.671 29.759** 63.429 16.444** 197.186 8.376** Error 28 1254.984 3.857 10.730 Total 39 7732.613 14.821 44.079 C.V. (coefficient variation) (%) 26,290 14,524 26,717 Root Height(cm) Chlorophyll(SPAD) Leaf (Number) Source of Var. df MS F value MS F value MS F value Replication 4 1.350 0.940ns 847.725 3.547* 3.025 0.199ns Fertilizer 7 40.971 28.537** 6595.682 27.601** 221.757 14.620** Error 28 1.436 238.968 15.168 Total 39 8.523 1442.353 51.003 C.V. (%) 33,557 23,218 19,647 **p<0.01,* p<0.05 Table 3. Application Averages on Plant Growth Parameters Plant Weight Plant Height Plant Diameter Control 110.400 C 21.200 E 15.000 C Pos. Cont. 144.200 C 22.800 DE 16.000 C PPMs 273.200 B 29.200 AB 23.400 B PPMs+Pos. Cont. 364.000 A 27.400 BC 29.600 A Microalgae 145.400 C 27.600 BC 28.400 AB Microal..+Pos. Cont. 154.400 C 26.600 BC 28.000 AB PGPB 148.000 C 24.800 CD 26.400 AB PGPB +Pos. Cont. 148.000 C 32.400 A 32.000 A L.S.D.(%) 61,913 3,432 5,725 Root Height Chlorophyll Leaf Control 4.200 D 102.800 C 26.200 C Pos. Cont. 5.000 D 117.600 C 27.200 C PPMs 8.200 C 160.800 B 42.800 A PPMs+Pos. Cont. 9.200 BC 179.600 AB 43.800 A Microalgae..+Pos. Cont. 12.000 A 166.000 B 40.200 AB Microalgae 10.600 AB 204.200 A 38.600 AB PGPB 8.800 BC 178.000 AB 34.200 B PGPB +Pos. Cont. 11.600 A 199.600 A 37.800 AB L.S.D.(%) 2,094 27,017 6,806 Table 4. Path Analysis Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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It determines the impact of these biofertilizers on lettuce yield and quality.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eYield Enhancement\u003c/strong\u003e: Research indicates that PPMs, PGPB, and Microalgae biofertilizers are effective in increasing lettuce yield. Their usage leads to higher yields compared to traditional fertilization methods.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eQuality Improvement\u003c/strong\u003e: The study demonstrates that biofertilizers contribute positively to improving the quality of lettuce. Observations show that leaves are healthier, juicier, and more flavorful.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eContribution to Soil Health\u003c/strong\u003e: PPMs, PGPB, and Microalgae biofertilizers are found to have positive contributions to soil health. They increase soil microbial diversity and enhance soil structure.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvironmental and Economic Benefits\u003c/strong\u003e: The use of biofertilizers is highlighted as environmentally sustainable, reducing chemical fertilizer usage and minimizing environmental impacts. Additionally, biofertilizers are economically beneficial for farmers.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eImportance of Application Methods and Dosages\u003c/strong\u003e: The study emphasizes that the effectiveness of biofertilizers depends on application methods and dosages. It underscores the importance of determining suitable application methods and dosages to achieve optimal results.\u003c/p\u003e"},{"header":"INTRODUCTION","content":"\u003cp\u003eLettuce (\u003cem\u003eLactuca sativa\u003c/em\u003e L.) stands as the sole member of the genus \u003cem\u003eLactuca\u003c/em\u003e cultivated on a global scale for commercial purposes. Primarily valued for its leafy foliage, lettuce has surged in popularity and is now a commonly consumed vegetable worldwide. Lettuce, including head lettuce, romaine lettuce, and curly lettuce, is one of the most consumed leafy vegetables (Shi et al., \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e2022\u003c/span\u003e). The primary concern associated with the traditional use of fertilizers in leafy vegetables, which are consumed in their leaf form, is the potential accumulation of nitrates, which can increase the risk of cancer (Bian et al., \u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e2020\u003c/span\u003e). The choice of fertilizer plays a crucial role in determining nitrogen availability and lettuce yield (Urra, Alkorta, Mijangos, \u0026amp; Garbisu, 2020). Increasing yield and quality of lettuce has potential contribution with Nitrogen content of fertilizers and it can be sourced from various sources, including inorganic fertilizers like urea, as well as organic alternatives such as blood meal, feather meal, compost, and farm manure (Smith \u0026amp; Hadley, 1989). While some growers may prefer inorganic nitrogen fertilizers due to their ease of application and faster nitrogen release compared to organic options. In the literature, there are reported studies relating to increase quality with many kind of these fertilizer sources that includes different Nitrogen sources which have crucial points. Soil-plant interactions are a key factor in the physiological activities of plants, and resistance to soil properties and climate change is also crucial as Nitrogen sources. Studies have shown that PGPR or microbial fertilizers can affect the growth conditions of specific microorganisms living in the soil, thus altering plant growth and development related with usage of atmosphere Nitrogen as a different N source. Beneficial microorganisms that can be applied as inoculants to increase soil microbial diversity can be defined as Plant Probiotic Microorganisms (PPMs) (de Souza Vandenberghe et al., \u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e2017\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eThe functions of PPMs include free nitrogen fixation, suppression of soil-borne pathogens, production of simple organic molecules for plant uptake, dissolution of insoluble nutrient sources, production of polysaccharides to improve soil aggregation, etc. Additionally, it has been reported that these PPMs applications can protect plant cells against oxidative damage and increase plant resistance to saline conditions. The importance of these biotechnological products for sustainable environmental improvement and eco-innovation has also been reported in many studies (Talaat, Shawky, \u0026amp; Botany, \u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e2014\u003c/span\u003e)\u003c/p\u003e \u003cp\u003eChemical fertilizers containing high levels of nitrogen, such as ammonium nitrate, ammonium sulfate, urea, and similar ones commonly used in traditional agriculture, cannot be used in organic farming (Fulya, SAĞLAM, \u0026amp; Journal, \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e2018\u003c/span\u003e). Therefore, providing the nitrogen needed by plants with alternative and organic solutions has become important. Animal-derived large and small livestock manures, worm castings, and bat guano are all viable options for providing nitrogen in organic farming practices (ABD RAHMAN, TINGGA, BUKHORI, ABDULLAH, \u0026amp; Technology, 2023). However, when these fertilizers are considered only as a nitrogen source, the necessity of annual application, difficulties in procurement, and application challenges arise (Tyagi, Ahmad, Malik, \u0026amp; Technology, 2022).\u003c/p\u003e \u003cp\u003eMoreover, bacterial solutions, i.e., microorganism applications can be a continuous solution as a self-renewing nitrogen source with the condition of establishing a certain population in the soil (Akimbekov et al., \u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2022\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eTherefore, it is necessary to introduce new products to the agricultural sector in this regard. This research is designed to investigate the effects of microbial products containing \u003cem\u003eBacillus subtilis\u003c/em\u003e, \u003cem\u003eBacillus megaterium\u003c/em\u003e, \u003cem\u003eBacillus amyloliquefaciens\u003c/em\u003e (Type 1: PGPR), \u003cem\u003eLactobacillus lactis, Lactobacillus cremoris, Lactobacillus acidophilus, Lactobacillus plantarum, Rhodopseudomonas palustris, Saccharomyces cerevisiae\u003c/em\u003e (Type 2: PPMs), \u003cem\u003eChlorella vulgaris\u003c/em\u003e (Type : Microalgae), on lettuce cultivation under greenhouse conditions, aiming to determine their effects on plant growth, yield, and some quality criteria.\u003c/p\u003e"},{"header":"MATERIAL and METHODS","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003e1.1. Cultivar of Cultivated Plant\u003c/h2\u003e \u003cp\u003eIn the research conducted within the greenhouse located at the Research and Application Field of Gebze Technical University, the cultivar Festival curly lettuce (\u003cem\u003eLactuca sativa\u003c/em\u003e L. var. Crispa) was used as the plant material. It is suitable for cultivation in every season. The plant has a robust structure with open green leaves, presenting an attractive appearance with its glossy foliage. Due to its numerous leaves, it has a large appearance, and its leaf edges are extremely curly. It has resistance to bolting and does not break during harvesting and washing due to its flexible leaf structure. It maintains leaf quality for a long time, thus having a long shelf life. It has high tolerance to tip burn. It is tasty with its juicy and crisp leaf structure. Suitable for greenhouse, low tunnel, and field production. The number of plants to be planted per hectare ranges from 5,500 to 7,000. Disease Resistance: BI: 1\u0026ndash;20, 22\u0026ndash;24 (HR) LMV: 2 (IR).\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec4\" class=\"Section2\"\u003e \u003ch2\u003e1.2. Trial Location and Characteristics\u003c/h2\u003e \u003cp\u003eThis study was conducted in the High Tunnel Greenhouse located within the Gebze Technical University Campus from December (2022) to March (2023). Gebze Technical University is located within the boundaries of the Gebze district. The trial coordinates were taken as Gebze Technical University - GPS coordinates 40\u0026deg; 48' 27.1764\" and 29\u0026deg; 21' 50.8536\". In the study, 3\u0026ndash;4 true leaf seedlings obtained from a private seedling company were arranged in the soil greenhouse with a drip irrigation system, with at least 10 plants per row and row spacing of 50 cm x 30 cm.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec5\" class=\"Section2\"\u003e \u003ch2\u003e1.3. Soil Characteristics\u003c/h2\u003e \u003cp\u003eSome physical and chemical properties of the soil in the region where the study was conducted are given in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e. Soil samples were taken from the greenhouse and sent to a special soil analysis laboratory. According to the analysis data, the soil structure is clayey loam, and the amount of organic matter and plant-available nitrogen, phosphorus, potassium, and trace elements were considered sufficient (Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). For crop growth, combined applications with farmer control and farmer practices, 500 g/ha\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e of NPK fertilizer (Quatro 18.18.18) were applied three times (monthly) via drip irrigation. Additionally, 2 L/ha\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e of liquid sulfur was applied to balance the calcareous structure. Lettuce plants can complete their growth period most healthily in sandy soils. When the soil texture was examined, it was found to have a clayey loam structure, which is suitable for lettuce cultivation.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec6\" class=\"Section2\"\u003e \u003ch2\u003e1.4. Soil Analysis\u003c/h2\u003e \u003cp\u003eSoil samples were taken (0\u0026ndash;30 cm, 30 subsamples) to determine some soil properties. After the soil samples have been taken, exchangeable cations was determined using ammonium acetate (Thomas et al., \u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e1982\u003c/span\u003e). and cation exchange capacities (CEC) were determined using sodium acetate\u0026mdash;ammonium acetate (Sumner \u0026amp; Miller, 1996). Total N was determined by the Kjeldahl method (Bremner, 1996), plant-available P was determined by using the sodium bicarbonate method (Olsen, \u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e1954\u003c/span\u003e). Electrical conductivity (EC) was measured in saturation extracts (Rhoades, 1996). Calcium carbonate and soil pH were determined as a method by (McLean \u0026amp; properties, 1983). Soil organic matter was determined using the Smith-Weldon method (Nelson \u0026amp; Sommers, 1996). Available Fe, Mn, Zn, and Cu in the soils were determined by DTPA methods (Lindsay \u0026amp; Norvell, \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e1978\u003c/span\u003e). Available B was analyzed using the azomethine-H (Wolf \u0026amp; Analysis, 1974).These soil characterization data are presented in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e \u003cp\u003eTable\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e. Some Physical and Chemical Properties of the Trial Soil (mean\u0026thinsp;\u0026plusmn;\u0026thinsp;standard deviation, n\u0026thinsp;=\u0026thinsp;16)\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec7\" class=\"Section2\"\u003e \u003ch2\u003e1.5. Experimental Design and Arrangement\u003c/h2\u003e \u003cp\u003eThe research was conducted in a high tunnel greenhouse as a pot trial, with a randomized complete block design consisting of 8 factors (Control, Positive Control, Microalgae, Microalgae\u0026thinsp;+\u0026thinsp;Positive Control, PPMs, PPMs\u0026thinsp;+\u0026thinsp;Positive Control, PGPB, PGPB\u0026thinsp;+\u0026thinsp;Positive Control), 5 replications (drip line order), and 7 plants per replication (per row). The obtained data were analyzed using IBM SPSS 23 software package, and statistical comparisons between the means were performed using one-way ANOVA and Duncan test. The statistical significance level was set at p\u0026thinsp;\u0026le;\u0026thinsp;0.05.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003e1.6. The Biofertilizers and Their Properties\u003c/h2\u003e \u003cp\u003eIn the trial three different biofertilizer formulations were used. The first group of biofertilizer has been formulated with Microalgae: \u003cem\u003eChlorella vulgaris\u003c/em\u003e (1x10\u003csup\u003e6\u003c/sup\u003e cfu/ml) and the second group of formulated biofertilizer is composed by PPMs: \u003cem\u003eLactobacillus lactis, Lactobacillus cremoris, Lactobacillus acidophilus, Lactobacillus plantarum, Rhodopseudomonas palustris, Saccharomyces cerevisiae\u003c/em\u003e (7x10\u003csup\u003e6\u003c/sup\u003e cfu/ml) and the third formulation is in PGPB group including \u003cem\u003eBacillus subtilis, Bacillus megaterium and Bacillus amyloliquefaciens\u003c/em\u003e (1x10\u003csup\u003e8\u003c/sup\u003e cfu/ml).\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec9\" class=\"Section2\"\u003e \u003ch2\u003e1.7. Type of Equipment Used\u003c/h2\u003e \u003cp\u003eTo ensure uniform distribution of fertilizers and no differences between applications, equal amounts of irrigation were applied every other day to match the field capacity of the soil in the greenhouse. Since no damage or disease was encountered during the cultivation period under tunnel greenhouse (temperature degree) conditions, no spraying was deemed necessary. Planting of curly lettuce (Festival) seedlings was done manually. Only the same amount of water was applied to the control groups where no product was applied. The entire area was subjected to fertilization conditions under the same farmer conditions. Periodically, weeds in the greenhouse soil were cleared using cultural methods. Product application was carried out using a graduated pump sprayer and fertilizer tanks, with the product mixed with water and applied via drip irrigation system. Plant diameters were measured using a caliper, and heights were measured using a meter at harvest. Plant weights were measured as fresh weight on sensitive scales (\u0026plusmn;\u0026thinsp;0.5 g). Chlorophyll values in plants were measured using a portable chlorophyll meter device from Scout brand. Greenhouse air humidity and temperature were measured using an Htc thermometer digital hygrometer temperature and humidity alarm device. Soil pH was measured using a Motorobit brand pH meter. According to the data obtained, the average pH in the untreated groups was found to be 7.4, while in the treated groups, the average pH decreased to around 6.6. It is known that ideal pH ranges for curly lettuce cultivation fall within these values.\u003c/p\u003e \u003cdiv id=\"Sec10\" class=\"Section3\"\u003e \u003ch2\u003e1.8. Application Timing and Frequency\u003c/h2\u003e \u003cp\u003eThe research was conducted in a high tunnel greenhouse as a pot trial, with a randomized complete block design consisting of 8 factors. \u003cb\u003eControl\u003c/b\u003e: No chemical or biological fertilizer application was made to the soil. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. \u003cb\u003ePositive Control\u003c/b\u003e: In farmer control applications, 500 g/ha of NPK fertilizer (Quatro 18.18.18) was applied three times per month via drip irrigation. Liquid sulfur application was made at a rate of 2 L/ha-1 to balance the calcareous structure. Additionally, organic base fertilizer was applied at a rate of 100 kg/ha to enrich the organic matter. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. \u003cb\u003ePPMs\u003c/b\u003e: Lettuce seedlings were planted by dipping the roots into a solution prepared by mixing 500 ml of PPMs in 100 liters of water before planting. The lettuce seedlings were irrigated with 2 liters of PPMs per decare on the first planting date, and irrigation was done every 15 days until harvest on May 5, 2023, with 1 liter of PPMs per decare. From planting to harvest, 200 ml of PPMs per 100 liters of water was sprayed on the leaves at weekly intervals. Applications were made on cool days when direct sunlight was not present. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. \u003cb\u003ePositive Control\u0026thinsp;+\u0026thinsp;PPMs\u003c/b\u003e: In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted by dipping the roots into a solution prepared by mixing 500 ml of PPMs in 100 liters of water. Lettuce seedlings were irrigated with 2 liters of PPMs per decare on the first planting date, and irrigation was done every 15 days until harvest on May 5, 2023, with 1 liter of PPMs per decare. From planting to harvest, 200 ml of PPMs per 100 liters of water was sprayed on the leaves at weekly intervals. Applications were made on cool days when direct sunlight was not present. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. \u003cb\u003ePGPB\u003c/b\u003e: In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted using drip irrigation with 3 L/da of Losepa prepared at a rate of 100 liters of water per 3 liters. Applications were made twice at 15-day intervals from the planting date to 15 days later, and then 3rd and 4th applications were made every 15 days using PGPB prepared with 3 L/da of water per 100 liters of water as spray from the leaves. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. \u003cb\u003ePositive Control\u0026thinsp;+\u0026thinsp;PGPB\u003c/b\u003e: In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted using drip irrigation with 3 L/da of PGPB prepared at a rate of 100 liters of water per 3 liters. Applications were made twice at 10-day intervals from the planting date to 15 days later, and then 3rd and 4th applications were made every 15 days using Losepa prepared with 3 L/da of water per 100 liters of water as spray from the leaves. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. \u003cb\u003eMicroalgae\u003c/b\u003e: In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted using drip irrigation with 1500 cc of Microalgae prepared at a rate of 1000 liters of water per da. Applications were made twice at 10-day intervals from the planting date to 14 days later, and then 3rd, 4th, and 5th applications were made every 14 days using Microalgae prepared with 1500 cc of water per 1000 liters of water as spray from the leaves. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row. \u003cb\u003ePositive Control\u0026thinsp;+\u0026thinsp;Microalgae\u003c/b\u003e: In addition to fertilization according to farmer conditions before planting, lettuce seedlings were planted using drip irrigation with 1500 cc of Microalgae prepared at a rate of 1000 liters of water per da. Applications were made twice at 10-day intervals from the planting date to 14 days later, and then 3rd, 4th, and 5th applications were made every 14 days using Microalgae prepared with 1500 cc of water per 1000 liters of water as spray from the leaves. The group was planned with 5 replications, each replication consisting of 7 lettuce plants per row.\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003e1.9. Meteorological Data\u003c/h2\u003e \u003cp\u003eMeteorological data obtained from the General Directorate of Meteorology for the period from December 15, 2022, to March 5, 2023, covering the trial area, showed minimum, maximum, and average temperatures of 3.5, 13, and 8.25\u0026deg;C, respectively. Throughout the vegetation period, the average minimum and maximum temperatures in the greenhouse ranged between 10\u0026ndash;15\u0026deg;C. Curly lettuce cultivation conforms to ideal standards 8\u0026ndash;20\u0026deg;C, and no additional heating or cooling was required in the greenhouse. Greenhouse humidity values were measured between 70\u0026ndash;75% using a humidity meter throughout the vegetation period, and ventilation was performed at certain intervals to maintain this humidity.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec12\" class=\"Section2\"\u003e \u003ch2\u003e1.10. Planting duration\u003c/h2\u003e \u003cp\u003ePlanting Date: November 15, 2022, Harvest Date: March 5, 2023, Vegetation Period: 80 days, Planting Conditions: GTU Soil Greenhouse Trial Drip Irrigation System\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec13\" class=\"Section2\"\u003e \u003ch2\u003e1.11. Measurements and Analyses\u003c/h2\u003e \u003cp\u003eThe characteristics and methods examined in the study are as follows:\u003c/p\u003e \u003cp\u003ePlant Height (cm/plant): At the completion of the trial, the region from the root collar to the growth tip was measured in centimeters (\u0026plusmn;\u0026thinsp;0.5 cm) using a meter in three randomly selected plants (per replication), Plant Diameter (cm/plant): The stem diameter was determined in millimeters (\u0026plusmn;\u0026thinsp;0.1 mm) using a digital caliper Number of Leaves Plant Weight (g), Root Length (cm/plant) (Pandey, Paul, Das, Meena, \u0026amp; Meena, 2017) Chlorophyll (SPAD): Chlorophyll levels in plants were measured using a portable chlorophyll meter device from Scout brand (Yin et al., \u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e2023\u003c/span\u003e).\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec14\" class=\"Section2\"\u003e \u003ch2\u003e2. STATISTICAL ANALYSIS\u003c/h2\u003e \u003cp\u003eThe data was analyzed by One-Way ANOVA with SPSS software (version 25.0). The Duncan test was used to determine meaningful differences between the means (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05 and p\u0026thinsp;\u0026lt;\u0026thinsp;0.01). PCA (Principal Component Analysis) was analysed on Minitab 17, Path Analysis was made with R studio program and Decision Tree Analysis was made with with SPSS software (version 25.0).\u003c/p\u003e \u003c/div\u003e"},{"header":"RESULTS AND DISCUSSION","content":"\u003cp\u003eSoil samples were analyzed with the following values: pH 7.3±0.17, EC 0.02±0.001 (dS/cm), calcium carbonate 1,7±0.15%, OM 1,65±0.14% (Table 1).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003c/strong\u003e Some Physical and Chemical Properties of the Trial Soil\u003c/p\u003e\n\u003cp\u003eThe variance analysis and application averages showing the interaction difference of fertilizer applications are given in Tables 2 and 3. According to the variance analysis, the effect on parameters such as plant weight, height, diameter, root length, chlorophyll content, and leaf count was found to be significant (p \u0026lt; 0.01), with the C.V% values generally ranging between 20 and 30 when considered. This indicates that application errors or deviations are within acceptable limits Upon examining the application averages (Table 3), it was observed that fertilizers exhibit a significant positive difference compared to the control group. Especially, applications where the positive control is combined with PGPB and PPMs have been found to yield better results compared to other groups. This indicates the active role of bacteria in the PGPB and PPMs groups in making the nutrients in the fertilizer beneficial to the plant. Similar results were also obtained with combined applications involving microalgae-containing fertilizers.\u003c/p\u003e\n\u003cp\u003eIn conclusion, applying these treatments along with chemical fertilizers in lettuce plants has improved plant photosynthetic efficiency, resulting in better plant yield and quality.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2. Analysis of Variance (ANOVA)\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 3. Application Averages on Plant Growth Parameters\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003ePath analysis, presented in Table 4, examines the effects of factors considered as dependent variables on plant weight. Path analysis is concerned with estimating the magnitude of connections between variables and providing information about the underlying causal processes (Simons, Conger, \u0026amp; Whitbeck, 1988). Upon examining Table 4, significant correlations at the 5% - 1% level were found among all the examined factors, except for the correlations between chlorophyll/plant weight and root length/plant weight.\u003c/p\u003e\n\u003cp\u003eWhen discussing the correlations between plant weight and other factors, it is observed that the highest correlation with plant weight is leaf count. According to path analysis, while the direct effect of plant height on plant weight is 11.3%, the highest indirect effect is between leaf count (35.946%) and root length (42.95%) with a total indirect effect of 78.896%. For plant diameter, the direct effect on plant weight is 5.63%, while the highest indirect effects are through leaf count (43.78%) and root length (38.035%) with a total indirect effect of 87.415%. The direct effect of leaf count on plant weight is 57.61%, while the highest indirect effect is through root length (0.85%) with a total indirect effect of 39.85%. The effect of chlorophyll content on plant weight is 39.93%, with the highest indirect effect through leaf count (40.639%) and root length (39.235%). The direct effect of root length on plant weight is 44.552%, with the highest indirect effect through chlorophyll content (39.235%).\u003c/p\u003e\n\u003cp\u003ePath analysis shows that the marketable value of the plant weight is mainly shaped under the influence of leaf count and root length. The vegetative weight, which expresses plant yield, is the result of a complex chain of activities involving all these factors. However, some factors are more significant than others, as evidenced by their importance relative to other factors (Saleh \u0026amp; environment, 2012). Accordingly, in our study, leaf count and root length have been shown to be the most influential factors affecting plant weight. Leaves are crucial organs containing metabolic activities involving both catabolic and anabolic processes, and they play a key role in photosynthetic activities and dry matter production. Similarly, roots are the most important part of the plant as they transport water and nutrients to the plant. As root length increases, the plant's attachment to the soil and uptake of water and nutrients also increase (Lai, Wang, Peng, \u0026amp; Chen, 2011). The application of PGPB, PPMs, and microalgae in conjunction with chemical fertilizers in our study positively affected leaf count and root length, the most influential factors affecting plant weight, thus, this combination can be safely used to increase plant yield.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 4. Path Analysis\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003ePrincipal component analysis can be summarized as a graphical method that compresses data obtained through classification, making it more understandable by reducing dimensions(ROTARU, POP, VATCĂ, CIOBAN, \u0026amp; Horticulture, 2012).The aim of this technique is to examine multidimensional data and express them with fewer variables based on fundamental characteristics (Eriksson, Byrne, Johansson, Trygg, \u0026amp; Vikström, 2013). Principal component analysis is widely used in agriculture to reliably demonstrate the relationship and performance of applied factors and examined elements (Granato et al., 2018). The factors examined in our study and their relationship with each other are shown in Figure 1. As seen in Figure 1, the examined elements and applied factors have formed three different groups.\u003c/p\u003e\n\u003col start=\"1\" type=\"1\"\u003e\n\u003cli\u003eGroup: Microalgae, Microalgae + positive control, PGPB + Positive control, leaf count, plant height, plant diameter, chlorophyll, and root weight.\u003c/li\u003e\n\u003cli\u003eGroup: Plant weight, PPMs, and PPMs + Positive Control.\u003c/li\u003e\n\u003cli\u003eGroup: Control, Positive Control, and PGPB.\u003c/li\u003e\n\u003c/ol\u003e\n\u003cp\u003eAlthough the applications PPMs and PPMs + Positive Control in Group 2 were identified as the most influential applications on plant weight, they only manifest themselves when the plant is healthy and when the maximum benefit from fertilizer is desired. In other words, the effectiveness of plant probiotics can be more possible when the plant is well-developed and healthy. In the first group mentioned (Microalgae, Microalgae + Positive Control, PGPB + Positive Control), the factors affecting plant weight, such as root length, chlorophyll count, plant diameter, plant height, and leaf count, are included in the same group, and this group has been identified as the most effective group on plant growth and yield. In other words, the most effective applications on plant weight are Microalgae, Microalgae + Positive Control, and PGPB + Positive Control. The individual applications of Positive Control and PGPB did not show the expected effect on plant growth. In conclusion, Microalgae, Microalgae + Positive Control, PGPB + Positive Control, PPMs + Positive Control, and PPMs can be recommended as the most effective groups for plant performance. The effectiveness of chemical fertilizers in use is known to be around 5-10% in the soil. \u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1. PCA Analysis \u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eDecision tree analysis is a method commonly used in social sciences but can also be applied in agricultural and natural sciences. The primary purpose of this analysis is to determine which main factors have the most influence on the dependent variable and how other factors are shaped by the scarcity or abundance of this main factor, ultimately revealing the numeric value of the dependent variable (Rajeswari, Suthendran, \u0026amp; Agriculture, 2019). The tree diagram illustrating the analysis of data obtained from the research is presented in Figure 2.\u003c/p\u003e\n\u003cp\u003eIn the analysis, it was determined that the main factor affecting the dependent variable, which is plant weight, is the leaf count. As the leaf count increases, the plant weight also increases. When the leaf count is less than 45, the plant weight is determined to be 166.743 grams, while it can reach an estimated plant weight of 320.4 grams when the leaf count exceeds 45. When the leaf count is less than 45, root weight comes into play as the main factor. As root length increases, so does plant weight. When the root length is between 3-4 cm, the estimated plant weight is 116.00 grams. Similarly, when the root length is between 3-4 cm, the main factor affecting plant weight is plant diameter. When the plant diameter is less than 16 cm, the estimated plant weight is 105.250 grams, whereas it is predicted to be 137.5 grams when the plant diameter exceeds 16 cm.\u003c/p\u003e\n\u003cp\u003eIn cases where the root length is 5,6,8 and 10-13 cm, chlorophyll amount emerges as the main factor. When the chlorophyll amount decreases below 163 SPAD, the estimated plant weight is 143.843 grams, while it is 209 grams when the chlorophyll amount is between 163-177, and 147.22 grams when the chlorophyll amount exceeds 177 SPAD. It is determined that the SPAD range of 163-177 provides the highest plant weight.\u003c/p\u003e\n\u003cp\u003eWhen the root length is between 7 and 9 cm, the leaf count emerges as the main factor, and as the leaf count increases up to a certain point, the estimated plant weight also increases. Based on the variation in leaf count, the estimated plant weight ranges from 151 grams when the leaf count is less than 35 to 265 grams when it exceeds 43.\u003c/p\u003e\n\u003cp\u003eAs a result, the main factor affecting plant weight is the leaf count, followed by root length. Additionally, plant diameter and chlorophyll are identified as significant factors depending on the increase or decrease of the two main factors. The conditions for achieving the highest plant yield are determined to be a leaf count between 40-43, root length between 7-9 cm, a relatively large plant diameter, and chlorophyll levels between 163-177 SPAD values.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Decision Tree Analysis \u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThere are many reviews offering an overview of the vast information about microorganisms, and specially bacteria, that are plant growth promoters and biocontrol agents, and also reviewing the mechanisms associated with these particular functions, as well as more publications showing and/or reviewing the benefits to plants, such as the enhancement of bioactive compounds in the edible parts, among others (Gouda \u0026amp; Saranga, 2018) (Etesami, Maheshwari, \u0026amp; safety, 2018; Menendez \u0026amp; Garcia-Fraile, 2017) (Chiboub, Jebara, Abid, \u0026amp; Jebara, 2020; Santoyo, Moreno-Hagelsieb, del Carmen Orozco-Mosqueda, \u0026amp; Glick, 2016).\u003c/p\u003e\n\u003cp\u003eBriefly, PPBs have features involved in the\u003c/p\u003e\n\u003cp\u003e(i) facilitation of nutrient acquisition,\u003c/p\u003e\n\u003cp\u003e(ii) production of phytohormones and modulation of their levels,\u003c/p\u003e\n\u003cp\u003e(iii) tolerance to either abiotic or biotic stresses,\u003c/p\u003e\n\u003cp\u003e(iv) production of siderophores and other metabolites, and\u003c/p\u003e\n\u003cp\u003e(v) induction of disease resistance, among\u003c/p\u003e\n\u003cp\u003eother properties not listed here.\u003c/p\u003e\n\u003cp\u003eThe use of plant probiotic bacteria as inoculants has the potential to increase crop yield without the overapplication of chemical fertilizers, pesticides, and fungicides, and consequently to reduce the environmental impact in agriculture and maximize the production of heathier and safer foods. PPB establish synergies and act complementarily when forming a consortium, providing beneficial effects to crops. Although a high number of these associations are well-documented and most of them show positive results, there is still a lack of knowledge on how these consortia behave and interact with plants, environments, and other the microbiome. The microbes that live in and on the plant microbiome are critical for plant health and exert their influence by facilitating the nutrient acquisition, regulating plant hormone levels, and helping to withstand pathogen attack. Plants are meta-organisms that are associated with complex microbiomes. The majority of the microorgansims including epiphytes and endophytes generally play a significant role in providing essential nutrients to the plants where they live.\u003c/p\u003e"},{"header":"CONCLUSION","content":"\u003cp\u003eAs a result of the study, it has been determined that biofertilizer applications significantly enhance plant health and development compared to the control groups. Especially, the efficiency of productivity, particularly with the application of PGPB, followed by Microalgae and PPMs, in combination with chemical fertilizers, has positively impacted the productivity and quality parameters examined in lettuce cultivation. The efficacy of chemical fertilizers is increased. The use of biological fertilizers in conjunction with chemical fertilizers enhances leaf count and root length, leading to an increase in chlorophyll content, plant diameter, and plant height. Therefore, with the use of combined fertilizers, an increase in both leaf count and root length results in higher plant yield per unit area. The highest plant yield is achieved when the leaf count is above 40, the root length is between 7\u0026ndash;9 cm, the plant diameter exceeds 16 cm, and the chlorophyll content is approximately between 160\u0026ndash;180 SPAD.\u003c/p\u003e \u003cp\u003eBiological fertilizers exhibit much better performance in terms of crop yield and quality when used in combination with chemical fertilizers rather than being used alone.\u003c/p\u003e \u003cp\u003eProbiotics are not only important for human health but also crucial for plant and soil health.\u003c/p\u003e \u003cp\u003eWe may conclude that the use of these beneficial bacteria combined in consortia is very important for improving lettuce yields and performance. However, the overall ecological impact is still not well-known and must be addressed in future studies if the successful and sustainable application of bacterial consortia is to be pursued for a better, greener, and more profitable agriculture.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003ePromoting Bacteria (PGPB), Plant Probiotic Microorganisms (PPMs)\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e: Not applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding:\u003c/strong\u003e This research was not funded\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting Interests:\u003c/strong\u003e We know of no conflicts of interest associated with this publication, and there has been no significant financial support for this work that could have influenced its outcome. As Corresponding Author, I confirm that the manuscript has been read and approved for submission by all the named authors\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics approval\u003c/strong\u003e: Not applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent to participate\u003c/strong\u003e: Not applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e: Not applicable\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eAll the studies were done by Assoc. Prof. Nurg\u0026uuml;l Kıtır Şen for this manuscript.\u003c/p\u003e\u003ch2\u003eAcknowledgement\u003c/h2\u003e\u003cp\u003eThanks to Binafert Biyoteknoloji Tarım ve Yazılım A.Ş. for supporting this study.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eABD RAHMAN, S.-S. N., TINGGA, R. C. T., BUKHORI, M. F. M., ABDULLAH, S. M. A. A. J. B. J. o. R. S., \u0026amp; Technology. (2023). 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Sustainable supply chains for supply chain sustainability: impact of sustainability efforts on supply chain risk. \u003cem\u003e56\u003c/em\u003e(17), 5820\u0026ndash;5835.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGranato, D., Santos, J. S., Escher, G. B., Ferreira, B. L., Maggio, R. M. J. T. i. F. S., \u0026amp; Technology. (2018). Use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) for multivariate association between bioactive compounds and functional properties in foods: A critical perspective. \u003cem\u003e72\u003c/em\u003e, 83\u0026ndash;90.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLai, W.-L., Wang, S.-Q., Peng, C.-L., \u0026amp; Chen, Z.-H. J. W. r. (2011). Root features related to plant growth and nutrient removal of 35 wetland plants. \u003cem\u003e45\u003c/em\u003e(13), 3941\u0026ndash;3950.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLindsay, W. L., \u0026amp; Norvell, W. J. S. s. s. o. A. j. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. \u003cem\u003e42\u003c/em\u003e(3), 421\u0026ndash;428.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMcLean, E. J. M. o. s. a. P. C., \u0026amp; properties, m. (1983). Soil pH and lime requirement. \u003cem\u003e9\u003c/em\u003e, 199\u0026ndash;224.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMenendez, E., \u0026amp; Garcia-Fraile, P. J. A. m. (2017). Plant probiotic bacteria: solutions to feed the world. \u003cem\u003e3\u003c/em\u003e(3), 502.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNelson, D. W., \u0026amp; Sommers, L. E. J. M. o. s. a. P. C. m. (1996). Total carbon, organic carbon, and organic matter. \u003cem\u003e5\u003c/em\u003e, 961\u0026ndash;1010.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eOlsen, S. R. (1954). \u003cem\u003eEstimation of available phosphorus in soils by extraction with sodium bicarbonate\u003c/em\u003e: US Department of Agriculture.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePandey, R., Paul, V., Das, M., Meena, M., \u0026amp; Meena, R. C. J. M. o. I. S. T. P. o. P. T. t. A. t. I. o. C. C. o. C. P. (2017). Plant growth analysis. 103\u0026ndash;107.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRajeswari, S., Suthendran, K. J. C., \u0026amp; Agriculture, E. i. (2019). C5. 0: Advanced Decision Tree (ADT) classification model for agricultural data analysis on cloud. \u003cem\u003e156\u003c/em\u003e, 530\u0026ndash;539.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRhoades, J. J. M. o. s. a. P. C. m. (1996). Salinity: Electrical conductivity and total dissolved solids. \u003cem\u003e5\u003c/em\u003e, 417\u0026ndash;435.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eROTARU, A. S., POP, I. 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Phytochemicals, nutrition, metabolism, bioavailability, and health benefits in lettuce\u0026mdash;A comprehensive review. \u003cem\u003e11\u003c/em\u003e(6), 1158.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSimons, R. L., Conger, R. D., \u0026amp; Whitbeck, L. B. J. J. o. D. i. (1988). A multistage social learning model of the influences of family and peers upon adolescent substance abuse. \u003cem\u003e18\u003c/em\u003e(3), 293\u0026ndash;315.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSumner, M. E., \u0026amp; Miller, W. P. J. M. o. s. a. P. C. m. (1996). Cation exchange capacity and exchange coefficients. \u003cem\u003e5\u003c/em\u003e, 1201\u0026ndash;1229.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTalaat, N. B., Shawky, B. T. J. E., \u0026amp; Botany, E. (2014). Protective effects of arbuscular mycorrhizal fungi on wheat (Triticum aestivum L.) plants exposed to salinity. \u003cem\u003e98\u003c/em\u003e, 20\u0026ndash;31.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eThomas, E. D., Sanders, J. E., Buckner, C. D., Papayannopoulou, T., Borgna-Pignatti, C., De Stefano, P.,.. . Storb, R. J. T. L. (1982). Marrow transplantation for thalassaemia. \u003cem\u003e320\u003c/em\u003e(8292), 227\u0026ndash;229.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTyagi, J., Ahmad, S., Malik, M. J. I. J. o. E. S., \u0026amp; Technology. (2022). Nitrogenous fertilizers: Impact on environment sustainability, mitigation strategies, and challenges. \u003cem\u003e19\u003c/em\u003e(11), 11649\u0026ndash;11672.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eUrra, J., Alkorta, I., Mijangos, I., \u0026amp; Garbisu, C. J. J. o. e. m. (2020). Commercial and farm fermented liquid organic amendments to improve soil quality and lettuce yield. \u003cem\u003e264\u003c/em\u003e, 110422.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWolf, B. J. C. i. S. S., \u0026amp; Analysis, P. (1974). Improvements in the azomethine-H method for the determination of boron. \u003cem\u003e5\u003c/em\u003e(1), 39\u0026ndash;44.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYin, H., Huang, W., Li, F., Yang, H., Li, Y., Hu, Y.,.. . Science, G. (2023). Multi-temporal UAV Imaging-Based Mapping of Chlorophyll Content in Potato Crop. \u003cem\u003e91\u003c/em\u003e(2), 91\u0026ndash;106.\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003e\u003cstrong\u003eTable 1.\u003c/strong\u003e Some Physical and Chemical Properties of the Trial Soil\u003c/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"432\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"56.94444444444444%\"\u003e\n \u003cp\u003e\u003cstrong\u003eSoil Properties\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.75%\" colspan=\"2\"\u003e\n \u003cp\u003e\u003cstrong\u003eUnit\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e\u003cstrong\u003eMeans\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eNitrogen(N)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003e%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e1,61\u0026plusmn;0.07\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003e*Organic Matter\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003e%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e1,65\u0026plusmn;0.14\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003ePlant available P\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003emg/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e4,31\u0026plusmn;0.48\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eExchangeable K\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003eCmolc/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e5,17\u0026plusmn;0.85\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eStructure\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003e%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e55,4\u0026plusmn;1.41\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003e*CaCO3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003e%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e1,70\u0026plusmn;0.15\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003epH (1:2 soil:water)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003e_\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e7,3\u0026plusmn;0,17\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003e*Electric Conductivity(EC)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003edS/cm\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e0,02\u0026plusmn;0,001\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eExchangeable Ca\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003eCmolc/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e17,5\u0026plusmn;1.41\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eExchangeable Mg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003eCmolc/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e2,58\u0026plusmn;0.23\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eExchangeable Na\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003eCmolc/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e0,83\u0026plusmn;0.12\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eAvailable Fe\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003emg/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e3,51\u0026plusmn;0.45\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eAvailable Zn\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003emg/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e11.4\u0026plusmn;0.51\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eAvailable Cu\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003emg/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e5,37\u0026plusmn;1.46\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eAvailable Mn\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003emg/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e3,58\u0026plusmn;1.36\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eCation Exchangeable Capacity\u0026nbsp;\u003c/p\u003e\n \u003cp\u003e(CEC)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003emeq/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e65,3\u0026plusmn;1.45\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eAvailable B\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003emg/kg\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e1,33\u0026plusmn;0.14\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eSand\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003e%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e35,31\u0026plusmn;1.45\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eClay\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003e%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e33,34\u0026plusmn;1.72\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"50%\"\u003e\n \u003cp\u003eSilt\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.694444444444443%\" colspan=\"2\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; %\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.305555555555557%\"\u003e\n \u003cp\u003e31,35 \u0026plusmn;2.13\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2.\u0026nbsp;\u003c/strong\u003eAnalysis of Variance (ANOVA)\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"100%\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.185567010309279%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.804123711340207%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003ePlant Weight(gr)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.804123711340207%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003ePlant Height(cm)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.804123711340207%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Plant Diameter(cm)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eSource of Var.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.185567010309279%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003edf\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003eMS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eF value\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eMS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003eF value\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eMS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003eF value\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eReplication\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.185567010309279%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e1251.413 \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e0.997ns \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e6.500 \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e1.685ns \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e9.588\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e0.893ns\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eFertilizer\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.185567010309279%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e37346.671 \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e29.759** \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e63.429 \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e16.444** \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e197.186\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e8.376**\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eError\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.185567010309279%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e28\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e1254.984\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e3.857\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e10.730\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eTotal\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.185567010309279%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e39\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e7732.613\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e14.821\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e44.079\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.402061855670103%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eC.V. (coefficient variation) (%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.185567010309279%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.804123711340207%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e26,290\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.804123711340207%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003e14,524\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.804123711340207%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e26,717\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"4.123711340206185%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.8659793814433%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003eRoot Height(cm)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.77319587628866%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003eChlorophyll(SPAD)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"27.835051546391753%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003eLeaf (Number)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003eSource of Var.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"4.2105263157894735%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003edf\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003eMS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003eF value\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003eMS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003eF value\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003eMS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003eF value\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003eReplication\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"4.2105263157894735%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e1.350\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003e0.940ns\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e847.725\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003e3.547*\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e3.025\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003e0.199ns\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003eFertilizer\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"4.2105263157894735%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e40.971 \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003e28.537**\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e6595.682\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003e27.601**\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e221.757\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003e14.620**\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003eError\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"4.2105263157894735%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e28\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e1.436\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e238.968\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e15.168\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003eTotal\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"4.2105263157894735%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e39\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e8.523\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.68421052631579%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e1442.353\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"14.736842105263158%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e51.003\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.631578947368421%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"13.402061855670103%\" valign=\"top\"\u003e\n \u003cp\u003eC.V. (%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"4.123711340206185%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.8659793814433%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e33,557\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.77319587628866%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e23,218\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"27.835051546391753%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003e19,647\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e**p\u0026lt;0.01,* \u0026nbsp; p\u0026lt;0.05\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003cstrong\u003eTable \u0026nbsp;3.\u0026nbsp;\u003c/strong\u003eApplication Averages on Plant Growth Parameters\u0026nbsp;\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"100%\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003ePlant Weight\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003ePlant Height\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003ePlant Diameter\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003eControl\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e110.400 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e21.200 E \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e15.000 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePos. Cont.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e144.200 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e22.800 DE \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e16.000 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePPMs\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e273.200 B \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e29.200 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e23.400 B \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePPMs+Pos. Cont.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e364.000 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e27.400 BC \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e29.600 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003eMicroalgae\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e145.400 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e27.600 BC \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e28.400 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003eMicroal..+Pos. Cont.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e154.400 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e26.600 BC \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e28.000 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePGPB\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e148.000 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e24.800 CD \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e26.400 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePGPB \u0026nbsp;+Pos. Cont.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e148.000 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e32.400 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e32.000 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003eL.S.D.(%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e61,913\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e3,432\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e5,725\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003eRoot Height\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003eChlorophyll\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003eLeaf\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003eControl\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e4.200 D \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e102.800 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e26.200 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePos. Cont.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e5.000 D \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e117.600 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e27.200 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePPMs\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e8.200 C \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e160.800 B \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e42.800 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePPMs+Pos. Cont.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e9.200 BC \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e179.600 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e43.800 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003eMicroalgae..+Pos. Cont.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e12.000 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e166.000 B \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e40.200 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003eMicroalgae\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e10.600 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e204.200 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e38.600 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePGPB\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e8.800 BC \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e178.000 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e34.200 B \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\"\u003e\n \u003cp\u003ePGPB \u0026nbsp;+Pos. Cont.\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e11.600 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e199.600 A \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e37.800 AB \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003eL.S.D.(%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e2,094\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e27,017\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25%\" valign=\"top\"\u003e\n \u003cp\u003e6,806\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003eTable \u0026nbsp;4.\u003c/strong\u003e Path Analysis\u003c/p\u003e\n\u003cp\u003e\u003cimg 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Growth-Promoting Bacteria (PGPB), Microalgae, Yield, Quality","lastPublishedDoi":"10.21203/rs.3.rs-4412276/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4412276/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe utilization of biofertilizers has emerged as a promising approach in modern agriculture to enhance crop productivity and quality while promoting environmental sustainability. In this study, we investigated the efficacy of three distinct biofertilizers, namely Potential Plant Probiotic Microorganisms (PPMs), Plant Growth-Promoting Bacteria (PGPB), and Microalgae, on the health on lettuce (\u003cem\u003eLactuca sativa\u003c/em\u003e L. var.\u003cem\u003eCrispa\u003c/em\u003e). The experiment was conducted in a controlled environment of a high tunnel greenhouse at Gebze Technical University. The study encompassed a comprehensive analysis of various growth parameters, including plant height, stem diameter, leaf count, plant weight, root length, and chlorophyll content related with plant health. Our findings demonstrate that the application of PPMs, PGPB, and Microalgae biofertilizers significantly improved lettuce yield and quality compared to traditional fertilization methods. Moreover, these biofertilizers positively influenced soil and plant health, contributing to enhanced microbial diversity and soil structure. Additionally, our study highlights the environmental and economic benefits associated with biofertilizer usage, paving the way for sustainable agricultural practices. Overall, this research underscores the potential of PPMs, PGPB, and Microalgae biofertilizers as effective alternatives in lettuce cultivation, offering promising prospects for future agricultural innovations and practices.\u003c/p\u003e","manuscriptTitle":"PPMs, PGPB and Microalgae as Three Distinct Biofertilizers on Lettuce Health","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-05-24 06:19:21","doi":"10.21203/rs.3.rs-4412276/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b7b23ae3-72eb-4501-a935-f4ffde5c4800","owner":[],"postedDate":"May 24th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-05-31T08:51:34+00:00","versionOfRecord":[],"versionCreatedAt":"2024-05-24 06:19:21","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4412276","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4412276","identity":"rs-4412276","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}