Small LEA proteins as an effective air-water interface protectant for fragile samples during cryo-EM grid plunge freezing
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Small LEA proteins from nematodes and tardigrades can protect fragile samples from air-water interface damage during cryo-EM grid plunge freezing, improving structural determination outcomes.
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Abstract
Sample loss due to air-water interface (AWI) interactions is a significant challenge during cryo-electron microscopy (cryo-EM) sample grid plunge freezing. We report that small Late Embryogenesis Abundant (LEA) proteins, which naturally bind to AWI, can protect samples from AWI damage during plunge freezing. This protection is demonstrated with two LEA proteins from nematodes and tardigrades, which rescued the cryo-EM structural determination outcome of two fragile multisubunit protein complexes.
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- europepmc
- last seen: 2026-05-20T01:45:00.602351+00:00