Expression and significance of VEGF, TGF-#beta#_(1), MCP-1 in ectopic and eutopic endometrium in patients with adenomyosis

Objective: To study the expression of vascular endothelial growth factor(VEGF), transforming growth factor-beta(TGF-β\-1), monocyte chemotactic protein-1(MCP-1) in ectopic and eutopic endometrium in patients with adenomyosis. Methods:Using immunohistochemistry SP method, the expression intensity of VEGF and TGF-β\-1 was examined in ectopic endometrium and eutopic endometrium,compared with normal endometrium. The expression of MCP-1 mRNA in these paraffin embedded specimens was examined by in situ hybridization technique. Results:(1) VEGF could express either in endometriumal glands or stromal cells. VEGF expressions in ectopic, eutopic endometrium with adenomyosis were both significantly higher than that of control group (P0.05); No statistically significant difference was found between in ectopic endometrium and eutopic endometrium (P0.05). (2) TGF-β\-1 could express either in endometriumal glands or stromal cells. TGF-β\-1 expression in ectopic,eutopic endometrium with adenomyosis was significantly higher than that of control group (P0.05). No statistically significant difference was found between in ectopic endometrium and in eutopic endometrium with adenomyosis(P0.05) (3) MCP-1 could express in three groups, MCP-1 expression in ectopic endometrium with adenomyosis was significantly higher than that of control group (P0.05). No statistically significant difference was found between in eutopic endometrium with adenomyosis and control group (P0.05). No statistically significant difference was found between in eutopic endometrium with adenomyosis and in ectopic endometrium (P0.05). (4) VEGF and TGF-β\-1 had the positive relativity in the ectopic endometriumal expression of adenomyosis patient (P0.05). VEGF and MCP-1 had the positive relativity in the ectopic endometriumal expression of adenomyosis patient (P0.05). Conclusions:VEGF and TGF-β\-1,MCP-1 can significantly express in ectopic endometrium which are the important cellular factors of promoting the vascular formation, VEGF and TGF-β\-1、VEGF and MCP-1 have the positive relativity in the ectopic endometrium. Data demonstrates that they could probably play important roles in the pathogenesis of adenomyosis.