MicroRNA expression profiles and networks in CXCL12-stimulated human endometrial stromal cells

Jie Mei, Ming-Qing Li, Ming‐Qing Li, Da‐Jin Li, Da-Jin Li, Haixiang Sun, Hai-Xiang Sun
Molecular medicine reports · 2016 · vol. 15(1) , pp. 249–255 · doi:10.3892/mmr.2016.5997 · PMID:27959395 · PMC5355667 · W2559760716
article OA: gold CC0 ⤵ 3 in-corpus citations
📄 Open PDF View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

This study identified differentially expressed microRNAs and mRNAs in CXCL12-stimulated endometrial stromal cells, building networks to reveal immune and inflammatory pathway involvement in endometriosis.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-06, 2026-06-10 ⓘ

This study examined how CXCL12 stimulation alters the microRNA and mRNA expression programs of primary human endometrial stromal cells (ESCs) and mapped the resulting miRNA–gene regulatory networks. Using miRNA and gene microarrays on ESCs from 18 women in the secretory phase (controls vs 48 h treatment with 100 ng/ml recombinant human CXCL12), the authors found 35 differentially expressed miRNAs and 1,671 differentially expressed mRNAs, and identified 63 intersection genes between miRNA predicted targets and the CXCL12-responsive mRNAs. Gene ontology and KEGG-based network analyses highlighted 39 significantly enriched biological processes, including immune cell chemoattractants, inflammatory/immune responses, and pathological processes of endometriotic lesions, but the paper does not report direct functional validation of individual miRNA or mRNA targets beyond computational network inference. This paper is centrally about endometriosis — it specifically studies CXCL12-induced miRNAome/mRNAome changes and miRNA–gene networks proposed to underlie CXCL12’s role in endometriosis.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

The chemokine stromal cell-derived factor-1 (C-X-C motif chemokine ligand 12; CXCL12) is important in the recruitment of leukocytes to the peritoneal cavity and the regulation of endometriotic tissue growth in endometriosis patients. However, the alterations in microRNA (miRNA) expression induced by CXCL12 remain to be fully elucidated. The present study evaluated key miRNAs in CXCL12‑stimulated endometrial stromal cells (ESCs), and investigated the underlying cellular regulatory mechanisms of CXCL12 in endometriosis by building networks between miRNAs, genes and gene ontologies (GOs). Differential expression of miRNAs and mRNAs induced by CXCL12 stimulation in ESCs was measured using miRNA and gene chips, and it was observed that 35 miRNAs and 1,671 mRNAs were differentially expressed. Using potential target genes of the 35 miRNAs, intersections of these genes were examined and 63 intersection genes were identified. A total of 39 GOs were obtained for these intersection genes, based on information from GO databases, including immune cell chemoattractants, inflammatory and immune responses, and pathological processes of endometriotic lesions in endometriosis. In addition, miRNA‑gene networks were built according to the GO and Kyoto Encyclopedia of Genes and Genomes databases. The present study, to the best of our knowledge, provides the most complete miRNAome and mRNAome profiles, and the most detailed investigation of the underlying cellular regulatory mechanisms, of the effects of CXCL12 in endometriosis. These results may facilitate the complete elucidation of the role of CXCL12 in endometriosis, and its underlying epigenetic mechanisms.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Chemokine CXCL12 Endometriosis Gene Regulatory Networks MicroRNAs Transcriptome Adult Cells, Cultured Chemokine CXCL12 Endometriosis Endometriosis Endometriosis Endometrium Endometrium Endometrium Endometrium Female Humans MicroRNAs Oligonucleotide Array Sequence Analysis RNA, Messenger

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (27)

Cited by (3)

Source provenance

europepmc
last seen: 2026-06-11T06:19:48.454388+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:20:43.714878+00:00
License: CC0 · commercial use OK