Adjunctive Therapy Using Ozonated Water and Ozonized Glycerin for Chronic Granulomatous Apical Periodontitis

Adjunctive Therapy Using Ozonated Water and Ozonized Glycerin for Chronic Granulomatous Apical Periodontitis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Case Report Adjunctive Therapy Using Ozonated Water and Ozonized Glycerin for Chronic Granulomatous Apical Periodontitis Shinichiro Yoshimine, Gotaro Shiota, Yoshimasa Makita, Hourei Oh This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8416245/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This case report describes a suspected case of chronic apical periodontitis caused by cementum detachment at the maxillary left lateral incisor. Treatment consisted solely of root surface debridement and curettage of pathological granulation tissue, and no periodontal regenerative therapy was performed. As adjunctive therapy, intraoperative irrigation with ozonated water was carried out, and ozonized glycerin was applied to the lesion site. On postoperative day 2, the clinical findings were as follows: no pain, swelling, or bleeding, with mild erythema; analgesic medication was not required. Inflammatory symptoms were well controlled from the early postoperative period, and additional antibiotic administration was deemed unnecessary. Sutures were removed on postoperative day 7. At the time of suture removal, mild wound dehiscence was observed at part of the incision; however, no bone exposure was noted. Two weeks postoperatively, proliferation of granulation tissue and regeneration of the oral mucosal epithelium were confirmed. At one month postoperatively, the surgical site had almost completely healed, and the incision line had become inconspicuous. Radiographic examination demonstrated a transition from radiolucency to radiopacity at the initial lesion site, indicating a healing tendency of the bone defect. No recurrence of infection was observed during the follow-up period, and a favorable healing course was achieved. These findings suggest that adjunctive therapy using ozonated water and ozonized glycerin may be effective for lesions suspected of chronic granulomatous apical periodontitis. Dentistry chronic granulomatous apical periodontitis ozonated water ozonized glycerin adjunctive therapy Full Text Additional Declarations The authors declare potential competing interests as follows: Gotaro Shiota is a researcher at Mediplus Pharma, Inc., the company that developed ozonized glycerin. Participant consent to publish Written informed consent was obtained from the patient for participation in this clinical case and for the publication of this case report. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8416245","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Case Report","associatedPublications":[],"authors":[{"id":563595748,"identity":"030895e6-f87d-4665-be49-a033f770b11a","order_by":0,"name":"Shinichiro Yoshimine","email":"","orcid":"","institution":"Yoshimine Dental Office","correspondingAuthor":false,"prefix":"","firstName":"Shinichiro","middleName":"","lastName":"Yoshimine","suffix":""},{"id":563595749,"identity":"a0960885-867f-4626-8af0-d1e6b6ac81b1","order_by":1,"name":"Gotaro Shiota","email":"","orcid":"","institution":"Mediplus 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to publish Written informed consent was obtained from the patient for participation in this clinical case and for the publication of this case report.\u003c/p\u003e","formattedTitle":"\u003cp\u003e\u003cstrong\u003eAdjunctive Therapy Using Ozonated Water and Ozonized Glycerin for Chronic Granulomatous Apical Periodontitis\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Osaka Dental University","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"chronic granulomatous apical periodontitis, ozonated water, ozonized glycerin, adjunctive therapy","lastPublishedDoi":"10.21203/rs.3.rs-8416245/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8416245/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis case report describes a suspected case of chronic apical periodontitis caused by cementum detachment at the maxillary left lateral incisor. Treatment consisted solely of root surface debridement and curettage of pathological granulation tissue, and no periodontal regenerative therapy was performed. As adjunctive therapy, intraoperative irrigation with ozonated water was carried out, and ozonized glycerin was applied to the lesion site. On postoperative day 2, the clinical findings were as follows: no pain, swelling, or bleeding, with mild erythema; analgesic medication was not required. Inflammatory symptoms were well controlled from the early postoperative period, and additional antibiotic administration was deemed unnecessary. Sutures were removed on postoperative day 7. At the time of suture removal, mild wound dehiscence was observed at part of the incision; however, no bone exposure was noted. Two weeks postoperatively, proliferation of granulation tissue and regeneration of the oral mucosal epithelium were confirmed. At one month postoperatively, the surgical site had almost completely healed, and the incision line had become inconspicuous. Radiographic examination demonstrated a transition from radiolucency to radiopacity at the initial lesion site, indicating a healing tendency of the bone defect. No recurrence of infection was observed during the follow-up period, and a favorable healing course was achieved. These findings suggest that adjunctive therapy using ozonated water and ozonized glycerin may be effective for lesions suspected of chronic granulomatous apical periodontitis.\u003c/p\u003e","manuscriptTitle":"Adjunctive Therapy Using Ozonated Water and Ozonized Glycerin for Chronic Granulomatous Apical Periodontitis","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-01-06 17:54:01","doi":"10.21203/rs.3.rs-8416245/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"d00c70cd-7f41-4627-87d8-23522b03a66d","owner":[],"postedDate":"January 6th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":60018100,"name":"Dentistry"}],"tags":[],"updatedAt":"2026-01-06T17:54:02+00:00","versionOfRecord":[],"versionCreatedAt":"2026-01-06 17:54:01","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8416245","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8416245","identity":"rs-8416245","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}