Synthesis of Fingolimod Loaded Sodium Alginate Nanoparticles and Investigation of Efficacy on Membrane Damage Induced Human U- 87 MG Glioma Cell

Synthesis of Fingolimod Loaded Sodium Alginate Nanoparticles and Investigation of Efficacy on Membrane Damage Induced Human U- 87 MG Glioma Cell | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Synthesis of Fingolimod Loaded Sodium Alginate Nanoparticles and Investigation of Efficacy on Membrane Damage Induced Human U- 87 MG Glioma Cell Buse PENCECI, Rabia CAKIR This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4589242/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The most common demyelinating disease is multiple sclerosis, and for the disease for which there is no curative treatment, there are only treatments used to reduce the frequency of attacks and symptoms. Fingolimod, one of the most important of these, is an immunomodulatory drug that acts as a sphingosine-1 phosphate receptor regulator and has many side effects. Nanoparticle-based drug delivery systems are modified as desired and given to the body, the dose and release time can be controlled. Sodium alginate is a biocompatible polysaccharide and preferred in nanoparticle formulations for drug delivery systems. It is aimed to encapsulation of fingolimod in alginate nanoparticles to reduce side effects and increase its effectiveness on membrane damage induced Human U-87 MG Glioma Cells. Nanoparticles with an average size of 106 nm were obtained by encapsulating Fingolimod with sodium alginate polymer and characterized by Dynamic Light Scattering (DLS), Scannig Electron Microscope (SEM), Atomic Force Microcope (AFM) and Fourier-Transform Infrared Spectroscopy (FTIR) analysis. Membrane damage was induced using lysophosphatidylcholine on the U-87 MG cell line. In order to demonstrate the efficacy of Fingolimod loaded sodium alginate nanoparticles on cells, cytotoxicity, neuroproliferative effect, neuroregenerativeness and neuroprotectivity analysis, apoptotic effect, cell migration, and cellular uptake were demonstrated. Within the scope of the study, the effectiveness of Fingolimod loaded sodium alginate nanoparticles, which have not been studied before in the literature, were examined and it was concluded that in addition to the limited symptomatic effects of Fingolimod mentioned in the literature, it also showed neuroregenerative, neuroprotective and neuroproliferative effects at the cellular level. This efficiency of Fingolimod has been increased by encapsulation of sodium alginate nanoparticles and has been studied at a level that can be a basis for future studies. MS neurodegenerative nanoparticle drug release Full Text Additional Declarations No competing interests reported. Supplementary Files Sunu1.pdf Highlights.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4589242","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":315175756,"identity":"187e3b67-532e-44f5-81d2-ec01138899c0","order_by":0,"name":"Buse PENCECI","email":"","orcid":"","institution":"Yildiz Technical University","correspondingAuthor":false,"prefix":"","firstName":"Buse","middleName":"","lastName":"PENCECI","suffix":""},{"id":315175757,"identity":"f16d1a30-028b-4a0a-96de-858814c04d47","order_by":1,"name":"Rabia CAKIR","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA3klEQVRIiWNgGAWjYFACHoYDQNKAjYEZREvIkKKFLQGkhYcoLSBgAGQYILj4gHn72YMHftTYGfNJn/n86kaNBQ8D++GjG/BpkTmTl3Cw51iyGRtf7jbrnGNAh/Gkpd3Ap0WCIcfgAG8Dsw0bD+824xw2oBYJHjP8WvjfGBz821AP1MLzzDjnHzFaJHIMDvM2HDYDamF+nNtGlJZ3CYdljh03ZuNhM2PO7ZPgYSPoF/7cwx/f1FQbzu9hfvw551udHD/74WN4tSADNgkwSaxyEGD+QIrqUTAKRsEoGDkAAKnWQFLPlN0TAAAAAElFTkSuQmCC","orcid":"","institution":"Yildiz Technical University","correspondingAuthor":true,"prefix":"","firstName":"Rabia","middleName":"","lastName":"CAKIR","suffix":""}],"badges":[],"createdAt":"2024-06-16 09:53:33","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4589242/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4589242/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":60682179,"identity":"9bc374b2-9d5d-4b51-a273-4a4746471bc5","added_by":"auto","created_at":"2024-07-19 12:51:03","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":992973,"visible":true,"origin":"","legend":"","description":"","filename":"SodiumAlginateNanoparticles.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4589242/v1_covered_3b5327a5-3a17-4f4b-8562-fc85fec30fa4.pdf"},{"id":59521439,"identity":"672cd256-3f6f-46ea-a07e-7d9e16e65093","added_by":"auto","created_at":"2024-07-02 19:31:43","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":500808,"visible":true,"origin":"","legend":"","description":"","filename":"Sunu1.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4589242/v1/4895e6f48c7299459984077e.pdf"},{"id":59521274,"identity":"1faab679-7045-4934-97bf-9fd620fdb283","added_by":"auto","created_at":"2024-07-02 19:23:43","extension":"docx","order_by":21,"title":"","display":"","copyAsset":false,"role":"supplement","size":15995,"visible":true,"origin":"","legend":"","description":"","filename":"Highlights.docx","url":"https://assets-eu.researchsquare.com/files/rs-4589242/v1/e916fb421d63cb55c31d5e4d.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Synthesis of Fingolimod Loaded Sodium Alginate Nanoparticles and Investigation of Efficacy on Membrane Damage Induced Human U- 87 MG Glioma Cell","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"MS, neurodegenerative, nanoparticle, drug, release","lastPublishedDoi":"10.21203/rs.3.rs-4589242/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4589242/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe most common demyelinating disease is multiple sclerosis, and for the disease for which there is no curative treatment, there are only treatments used to reduce the frequency of attacks and symptoms. Fingolimod, one of the most important of these, is an immunomodulatory drug that acts as a sphingosine-1 phosphate receptor regulator and has many side effects.\u003c/p\u003e \u003cp\u003eNanoparticle-based drug delivery systems are modified as desired and given to the body, the dose and release time can be controlled. Sodium alginate is a biocompatible polysaccharide and preferred in nanoparticle formulations for drug delivery systems.\u003c/p\u003e \u003cp\u003eIt is aimed to encapsulation of fingolimod in alginate nanoparticles to reduce side effects and increase its effectiveness on membrane damage induced Human U-87 MG Glioma Cells. Nanoparticles with an average size of 106 nm were obtained by encapsulating Fingolimod with sodium alginate polymer and characterized by Dynamic Light Scattering (DLS), Scannig Electron Microscope (SEM), Atomic Force Microcope (AFM) and Fourier-Transform Infrared Spectroscopy (FTIR) analysis. 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