Seasonal host shifts for Legionella within an industrial water-cooling system

Seasonal host shifts for Legionella within an industrial water-cooling system | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 18 January 2025 V1 Latest version Share on Seasonal host shifts for Legionella within an industrial water-cooling system Authors : Suzanne Crull 0000-0001-9516-0119 , Emlyn Hammer , Allison Mann , Lauren O'Connell , Ashlyn Soule , Elizabth Griffith , Thomas Blouin , Robin Brigmon , and Vincent Richards [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.173720490.07778934/v1 Published Environmental Microbiology Reports Version of record Peer review timeline 207 views 94 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Legionella is a genus of environmental bacteria containing pathogenic species such as Legionella pneumophila that are responsible for potentially fatal respiratory infections including Legionnaires’ disease and Pontiac fever. Disease etiology can involve Legionella replication intracellularly with protists and this study aimed to characterize the Legionella -protist relationship with the goal of developing novel outbreak prevention strategies. Water and sediment samples were collected from a water-cooling tower in South Carolina over a 6-month period. Concomitantly, multiple environmental parameters were recorded. Bacterial and eukaryotic communities were characterized using the 16S rRNA gene V4 region and a 252 bp fragment of the 18S rRNA gene, respectively. Co-occurrence network analyses were performed to elucidate Legionella -protist correlations through time. We found that Legionella correlated with different protists as the seasons progressed. Acanthamoeba correlated with Legionella in early spring followed by Vannella and Korotnevella in late spring and early summer, joined by Echinamoeba in mid-summer. Vannella and Acanthamoeba are known potential hosts for Legionella , while Korotnevella is a potential undocumented host. Of the environmental parameters, temperature showed a strong correlation with protist species, suggesting that Legionella frequency was driven by temperature-dependent protist availability. Understanding which protists aid Legionella persistence in water systems will advance our knowledge of the pathology of Legionnaire’s disease and provide potential targets to help predict and prevent disease outbreaks. Supplementary Material File (fig_2.eps) Download 23.42 MB File (main_text.docx) Download 11.37 MB Information & Authors Information Version history V1 Version 1 18 January 2025 Peer review timeline Published Environmental Microbiology Reports Version of Record 30 Jun 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords acanthamoeba legionella metataxonomics protists vannella water-cooling tower Authors Affiliations Suzanne Crull 0000-0001-9516-0119 Clemson University View all articles by this author Emlyn Hammer Clemson University View all articles by this author Allison Mann University of Wyoming View all articles by this author Lauren O'Connell Clemson University View all articles by this author Ashlyn Soule Clemson University View all articles by this author Elizabth Griffith Clemson University View all articles by this author Thomas Blouin Clemson University View all articles by this author Robin Brigmon Savannah River National Laboratory View all articles by this author Vincent Richards [email protected] Clemson University View all articles by this author Metrics & Citations Metrics Article Usage 207 views 94 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Suzanne Crull, Emlyn Hammer, Allison Mann, et al. Seasonal host shifts for Legionella within an industrial water-cooling system. Authorea . 18 January 2025. DOI: https://doi.org/10.22541/au.173720490.07778934/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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