Koisio Technology-Modulated Cell Culture Media can Significantly Increase the Antioxidant Capacity of Mouse Fibroblasts

Oxidative stress is not only a critical common pathological factor of numerous diseases, but also an important factor in the aging process. It is highly valuable to discover novel and economic substances for enhancing the antioxidant capacity of human body. In this study we used a cell culture model to determine the antioxidant capacity of Koisio technology-modulated solutions without additions of any external substances. Our study has obtained the following findings: First, the cells cultured in Koisio technology-modulated cell culture media (KM) showed significantly greater capacity to decrease H 2 O 2 -induced oxidative stress, compared with the cells cultured in regular cell culture media; second, the cells cultured in KM showed significantly greater capacity to resist H 2 O 2 -induced decreases in cell number, compared with the cells cultured in regular cell culture media; and third, the H 2 O 2 prepared in Koisio technology-modulated MEM produced significantly lower levels of decreases in cell number, compared with the H 2 O 2 prepared in MEM. Collectively, our study has indicated that Koisio technology can increase the antioxidant capacity of both solutions and cells without additions of any external substances. Based on our previous findings, we proposed that Koisio technology-modulated solutions increase cellular antioxidant capacity at least partially by increasing intracellular ATP levels.