Development of experimental endometriosis is dependent upon implant miR-451 expression and not host expression.

A. Endometriosis was induced in wild-type and miR-451 null mice using donor tissue from both wild-type and miR-451 null mice and the number of implants which developed was assessed as described under “Materials and Methods.” Data are displayed as the mean ± SEM. Different letters indicate statistically significant differences among the means as determined by one-way ANOVA followed by post-hoc analysis (N = 6 per group). B. miR-451 wild-type and not miR-451 deficient implants develop in the same wild-type host. Endometriosis was induced as described under “Materials and Methods” in wild-type host mice using both miR-451 wild-type (expressing EGFP) and miR-451 deficient (non-EGFP) endometrial fragments and implant establishment was assessed. Data are displayed as the mean ± SEM. Different letters indicate statistically significant differences among the means as determined by unpaired t-test (N = 6 per group).