A self-lysed attenuated Salmonella platform with flagellar displaying mediates tumor therapy

A self-lysed attenuated Salmonella platform with flagellar displaying mediates tumor therapy | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article A self-lysed attenuated Salmonella platform with flagellar displaying mediates tumor therapy Qingke Kong, Mengru Li, Xin Chen, Longlong Cao, Meihong Lei, Haoxiang Yuan, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7412577/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Conventional cancer therapies such as radiotherapy, chemotherapy, and surgery have obvious limitations in treating solid tumors. Bacteria-mediated tumor therapy has been proven effective in clinical studies and considered to be a promising strategy for tumor treatment. Salmonella, as a facultative anaerobic bacterium, can preferentially colonize tumors and activate the anti-tumor immune response of the host by remodeling the tumor microenvironment. Genetically engineered Salmonella have good tumor targeting ability and controllability, and are highly adaptable to different therapeutic requirements, thus serving as ideal carriers for drug delivery. In this study, RGD4C short peptide was displayed on the constitutively expressed flagellum of attenuated Salmonella Typhimurium to develop a vector suitable for fast moving and targeting and deep colonizing within hypoxic region in tumor. At the same time, in order to safely and effectively exert the anti-tumor activity in Salmonella, a regulated lysis system based on the new lysing protein M4lys encoded by phage was applied to lyse Salmonella peptidoglycan to release endogenous and exogenous anti-tumor molecules, and balanced lethal vector-host system was used to deliver exogenous anti-tumor molecules. In all, a novel genetically engineered live Salmonella was successfuly constructed and able to deliver exogenous anti-tumor molecules to exert the anti-tumor activity for tumor therapy. Biological sciences/Microbiology Biological sciences/Cancer Salmonella Typhimurium tumor therapy targeting cell self-lysed bacteria M4lys L-ASNase Full Text Additional Declarations There is NO Competing Interest. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7412577","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":523834352,"identity":"6cb83b07-7614-4961-b65c-9203ad811a3f","order_by":0,"name":"Qingke 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