The 9th International Conference on Biomedical Engineering and Pharmaceutical Sciences (ICBEPS2024).

Background

The purpose of this study was to investigate the effect of meibomian gland massage therapy in patients with meibomian gland dysfunction.

Methods

During the period from June 2022 to June 2023, our research team meticulously selected 100 patients diagnosed with meibomian gland dysfunction (MGD) who were undergoing treatment at our hospital. These patients were carefully chosen to form a comprehensive study sample, aiming to evaluate the efficacy of different treatment approaches. Utilizing a random number table method, we evenly allocated these patients into two distinct groups: a control group and an observation group, ensuring that each group consisted of 50 participants. This method of randomization was employed to eliminate any potential bias and to ensure that both groups were demographically and clinically comparable, thereby enhancing the validity of our study outcomes. The control group received standard eye care, which typically involves measures such as lubrication to alleviate dryness, and general hygiene practices aimed at maintaining ocular health. This routine care serves as the baseline treatment against which the effectiveness of additional interventions can be measured. Meibum gland massage is a therapeutic technique designed to express the meibum from the glands, which is particularly beneficial for patients with MGD as it helps to clear blockages and improve the quality and quantity of the secretions. The eyelid margin score (LMS), corneal fluorescein staining score (FLS), tear film break-up time (BUT) and symptom scores were compared between the two groups.

Results

The FLS score, LMS score, BUT and symptom score of the patients in the observation group were higher than those in the control group (P<0.05).

Conclusion

Meibomian gland massage treatment can not only improve the glandular function of meibomian gland dysfunction patients and alleviate the symptoms of dry eyes, but also effectively enhance their quality of life. This approach is particularly beneficial for those experiencing discomfort and irritation, as it can lead to a significant reduction in eye strain and fatigue, thus contributing to a more comfortable and productive daily living experience. BEPS24002: STUDY ON THE CARDIAC FUNCTION AND ANTIOXIDANT CAPACITY IN DIABETES PATIENTS WITH HEART FAILURE TREATED BY DAPAGLIFLOZIN Junhua Wanga, Lijuan Sunb aDepartment of Internal Medicine, Handan Aiyan Hospital, Handan 056000, Hebei, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

Diabetes in China exhibits high incidence, complication, and disability rates, severely impacting patient health. Type 2 diabetes predominates, with irregular blood sugar levels often leading to cardiovascular complications. Heart failure is a serious clinical complication. Dapagliflozin, a novel antidiabetic drug, has shown significant efficacy in treating diabetic heart failure. Recent studies have increased, offering new treatment options for patients. This study aims to evaluate the clinical effects, efficacy, and safety of dapagliflozin in treating diabetes and heart failure.

Methods

We selected 200 diabetes heart failure patients from our hospital’s internal medicine department between September 2021 and December 2023. Patients were divided into two groups based on randomized trials: the observation group received dapagliflozin along with standard care, while the control group received only standard treatment. Treatment efficiency, cardiac function indicators, antioxidant capacity, and adverse reaction probability during treatment were collected and recorded for both groups. Additionally, quality of life was assessed for both groups.

Results

Significant statistical differences were observed in treatment efficiency, cardiac function indicators, and antioxidant levels between the two groups before and after treatment (P<0.05). The treatment efficiency of the observation group was higher than that of the control group post-treatment, showing a significant statistical difference (P<0.05).

Conclusion

This study compared the application effects of dapagliflozin and basic treatment in patients with diabetes combined with heart failure, finding that dapagliflozin significantly improved treatment efficiency, cardiac function indicators, and antioxidant capacity. Although there was no significant difference in the incidence of adverse reactions between the two groups during treatment, the treatment efficiency of the observation group was higher than that of the control group, indicating that dapagliflozin has good efficacy and safety in clinical applications. However, due to the limited sample size, these results need to be validated in larger clinical trials. Future research could further explore the impact of different doses or combination therapy on patients with diabetes and heart failure, aiming to provide more treatment options and scientific evidence for clinical practice. Additionally, this study also suggests that when administering dapagliflozin, close monitoring of patient condition changes and potential adverse reactions is essential to ensure treatment safety and effectiveness. BEPS24003: CLINICAL EFFECT OF MAXILLARY EXPANSION DEVICE COMBINED WITH MUSCLE FUNCTIONAL APPLIANCE IN THE TREATMENT OF MALOCCLUSION IN CHILDREN Yanyan Duana aDepartment of Stomatology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

Childhood malocclusion is a common disease in the field of orthodontics, which not only affects the oral function and facial aesthetics of children, but may also have negative effects on their mental health. Therefore, finding effective treatment methods is of great significance for improving the quality of life of pediatric patients. This project observes the application of early functional training combined with orthodontic appliances in children with malocclusion.

Methods

A total of 120 pediatric patients with malocclusion treated at Handan Aiyan Ophthalmological Hospital were selected. The observation group was treated with a maxillary expansion device combined with a muscle function appliance for pediatric malocclusion, while the control group was treated with a maxillary expansion device alone. After treatment, the jaw structure and oral CBCT indicators of the two groups were compared.

Results

Before treatment, there were no statistically significant differences between the two groups in terms of SNA, SNB, ANB, FH-MP, SN-MP, Ptm-ANS male, and Ptm-ANS female levels (P>0.05). After treatment, the levels of SNA, SNB, ANB, FH-MP, SN-MP, Ptm-ANS male, and Ptm-ANS female increased in both groups, with the observation group showing higher levels than the control group, and the difference was statistically significant (P<0.05). Before treatment, there were no statistically significant differences between the two groups in terms of sagittal diameter (PL1, PL2, PL3), coronal diameter (PL1, PL2, PL3), cross-sectional area (S1, S2, S3), and segment volumes (V1, V2, V3) (P>0.05). After treatment, the sagittal diameters (PL1, PL2), coronal diameters (PL1, PL2), cross-sectional areas (S1, S2), and segment volumes (V1, V2) improved in both groups, while the sagittal diameter (PL3), coronal diameter (PL3), cross-sectional area (S3), and segment volume (V3) decreased. However, the changes in the observation group were greater than those in the control group, and the difference was statistically significant (P<0.05). The overall effectiveness rate in the observation group was 96.67% (58/60), which was higher than that in the control group at 76.67% (46/60), and the difference was statistically significant (P<0.05).

Conclusion

The use of a maxillary expansion device combined with a muscle function appliance in treating pediatric malocclusion can significantly improve the condition and is worthy of clinical promotion and application. BEPS24004: INTELLIGENT DIAGNOSIS SYSTEM FOR YOUNG AUTISTIC CHILDREN BASED ON ENSEMBLE LEARNING Jia Yaoa aShandong University, Qingdao, Shandong Province, People’s Republic of China

Background

The incidence rate of autism is increasing year by year, the academic community unanimously believes that the earlier a patient be intervened in rehabilitation training, the better the prognosis will be, so the early screening and diagnosis of autism have important academic value and practical significance, and the application of artificial intelligence technology combined with medical big data in the field of mental illness diagnosis is also a current hot research topic. Subjects and Methods: Ensemble learning can reduce the risk of over-fitting and significantly improve the overall performance of the model by integrating the prediction results of multiple weak learners. This study uses ensemble learning algorithms to train an intelligent diagnostic system for children under the age of three with autism based on a multimodal feature data space of young children. The algorithm obtains a subset of samples through operations on the sample set, and then trains a series of base classifiers on the subsets using weak classification algorithms. The basic idea of the training process is to use a convex error function for gradient descent in the function space to continuously optimize the prediction accuracy, and finally weight and fuse the generated weak classifiers obtain a strong classifier.

Results

This method can utilize the different perspectives of various models on data, thereby improving the accuracy of overall predictions, making it particularly suitable for handling complex data and tasks related to psychology. Compared with manual diagnosis and ordinary intelligent algorithms, its prediction accuracy can be improved to 96.97%, and it is superior to traditional algorithms in precision, recall, F1 score, ACU and other indicators, further demonstrating the stability and robustness of the algorithm, also displayed that the intelligent diagnostic system can still maintain stable operation when facing changes in internal structure or external environment.

Conclusions

This algorithm can lower the diagnosis age of autism to below 2 years old, which is lower than the 4-years old diagnosis age standard published by the Centers for Disease Control and Prevention in the United States. At the same time, it has good interpretability for clinical applications, can quantitatively express the weight of jaundice, gender, race, genetics and other characteristics during the decision-making process. BEPS24005: EFFECTS OF PHARMACEUTICAL CARE OF ORAL HYPOGLYCEMIC DRUGS COMBINED WITH WEIGHT REGULATION INTERVENTION ON SELF MANAGEMENT IN COMMUNITY-DWELLING ELDERLY WITH TYPE 2 DIABETES MELLITUS Hongzhao Wanga, Jie Hea, Ming Huob, Hong Duc, Ailing Wena, Jing Zhanga aDepartment of Physical Education, Huainan Normal University, Huainan, China,bSchool of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao, China,cDepartment of Physical Education, Anhui University of Science & Technology, Huainan, China.

Objective

It is preliminary reckoned that the number of people developed type 2 diabetes mellitus (T2DM) continues to increase and is projected to reach over 600 millions in the world by 2045. Direct health expenditure related to T2DM is no doubt a heavy medical burden, and is projected to be 825 billion USD in 2030. Direct health expenditure related to T2DM is no doubt a heavy medical burden. The purpose of this study was to investigate the effects of pharmaceutical care and body weight regulation on self management in community-dwelling elderly with Type 2 Diabetes (T2DM). Subjects and Methods: Cluster random sampling was used to select 1178 participants from community-dwelling residents aged over 60 years with T2DM. They were randomly divided into two groups according to different nursing intervention methods. The control group (n=589) received routine pharmaceutical care intervention, and the study group (n=589) received pharmaceutical care combined with weight regulation intervention. Demographic data, relational questionnaires and information were dispatched before and after intervention. Body weight and height, waistline, self-management were compared between study group and control group.

Results

The scores of self-management after intervention in the study group were more excellent than that of control group, the difference was statistically significant; the body weight and waistline were lower than that of control group, the difference was statistically significant. The rational use and compliance of oral anti-diabetes drug, and self-management could be improved by pharmaceutical care. Moreover, the multidimensional intervention of pharmaceutical care combined with weight regulation can effectively strengthen the self-management ability of community-dwelling elderly with T2DM, promote their disease control, and further ameliorate the quality of life of elderly, which is of high effect for community health promotion.

Conclusions

The rational use and compliance of anti-diabetes drug, and self-management could be improved by pharmaceutical care. Moreover, the multidimensional intervention of pharmaceutical care combined with weight regulation can effectively strengthen the self-management ability of community-dwelling elderly patients with T2DM, promote their disease control, and further improve the QoL of patients, which is of high value for clinical promotion. Further research is urgent to determine how to major the pharmaceutical care combined with weight regulation for this large and heterogeneous population. Acknowledgments: This work was supported by a project grant from the Natural Science Foundation of China (Grant No.82372585). BEPS24006: TO OBSERVE THE LIFTING EFFECT OF ULTRA-PICOSECOND LASER COMBINED WITH MICRONEEDLE INTRODUCTION ON MELASMA Suwei Liua aDepartment of Medical Cosmetology, Handan Aiyan Ophthalmology Hospital, Handan, Hebei 056000, China.

Background

To observe the lifting effect of ultra-picosecond laser combined with microneedling on the treatment of melasma.

Methods

A total of 100 melasma patients received by Handan Aiyan Eye Hospital from October 2022 to October 2023 were selected and divided into two groups according to the random number table method: the control group was treated with ultra-picosecond laser (n=50), and the observation group was treated with ultra-picosecond laser for 2 weeks and then given microneedle introduction therapy (n=50). The skin physiological indexes, skin lesion color and area, and clinical efficacy of the two groups of melasma patients were comprehensively observed and evaluated.

Results

The total effective rate of the observation group was significantly higher than that of the control group, and the difference was statistically significant (P<0.05), indicating a more pronounced therapeutic success in the observation group. Moreover, the skin physiological indexes of the observation group were significantly improved compared with the control group, with a significant difference (P<0.05), which underscores the positive impact on skin health parameters. Additionally, the color and area scores of skin lesions in the observation group were lower than those in the control group, and the difference was significant and statistically significant (P<0.05), reflecting a more effective reduction in the visibility and extent of skin lesions.

Conclusion

The combination of ultra-picosecond laser and microneedle introduction in the treatment of melasma can effectively repair facial skin damage, optimize skin physiological indicators, and have obvious lifting effects. This integrated approach targets the reduction of melanin pigmentation, which is central to melasma treatment. By leveraging the precision of ultra-picosecond laser technology, the treatment can specifically address pigmented lesions with minimal damage to surrounding tissues, leading to a more controlled and efficient breakdown of pigments. The microneedle introduction facilitates the penetration of topical agents, potentially including those that inhibit melanin production or promote skin lightening, thus enhancing the overall therapeutic outcome. This method not only improves the aesthetic appearance by reducing hyperpigmentation but also strengthens the skin’s structural integrity and its natural defenses, contributing to a more youthful and revitalized complexion. BEPS24007: RESEARCH ON THE DISTRIBUTION PATTERN OF TRADITIONAL CHINESE MEDICINE SYNDROMES IN OVERWEIGHT/OBESE PATIENTS WITH DYSLIPIDEMIA AND THE STUDY ON LIFESTYLE Zihao Zhanga, Yongxin Guanb, Shuyu Caoc, Qiyu Liua, Ruixi Xia, Jianqing Jua, Lizhi Lia, Guoju Donga, Hao Xua aNational Clinical Research Center for Chinese Medicine Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China, bDepartment of Palliative Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan, cBeijing Changping Hospital of Chinese Medicine, Beijing, China. ZZ, YG and SC contributed equally to this study.

Background

To analyze the distribution patterns of syndrome types in patients with overweight/obesity combined with dyslipidemia and their relationship with lifestyle habits. Subjects and Methods: A retrospective analysis was conducted on 403 patients from the Cardiovascular Department of Xiyuan Hospital, China Academy of Chinese Medical Sciences, between December 2021 and December 2023. The study examined patients’ demographic information, physical indicators, medical records, questionnaires, and laboratory test results.

Results

A total of 403 subjects were included in the study, comprising 165 males and 238 females. The median age was 43, and the median BMI was 29.17 kg/m2. The syndrome of spleen deficiency and dampness excess was the most common in patients with overweight/obesity and dyslipidemia, accounting for 44.7%. Additionally, patients with this syndrome had significantly higher BMI compared to other syndrome types and were younger in age of onset. There was also a significant association with lifestyle factors such as having fatty liver, not eating breakfast, eating quickly, and a sedentary lifestyle. Logistic regression analysis further confirmed the relationship between these lifestyle factors and the development of spleen deficiency and dampness excess syndrome.

Conclusions

More than 80% of overweight/ obese patients with dyslipidemia have deficiency syndrome or a combination of deficiency and excess syndrome types, which require supplementing deficiency or removing excess. The results of this study suggest that the combination of traditional Chinese medicine syndrome differentiation and lifestyle intervention in clinical treatment of overweight/ obesity combined with dyslipidemia is expected to achieve better therapeutic effects. Acknowledgments: Central Public Welfare Research Institutes of China Academy of Chinese Medical Sciences(ZZ13-YQ-017-C1). BEPS24008: THE FIRST CONGENITAL MESOBLASTIC NEPHROMA WITH PANCREATOBLASTOMA CASE REPORT, ABOUT ITS CLINICAL IMAGING FINDINGS Minggang Yia,b, Jianshe Zhaoa,b, Ke Yua,b aDepartment of Radiology, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong, China,bDepartment of Radiology, Jinan Children’s Hospital, Jinan, Shandong, China.

Background

Congenital mesoblastic nephroma (CMN) is a comparatively uncommon renal tumour affecting infants, with an incidence rate of just 8 cases per one million births. Similarly, pancreatoblastoma (PBL) is an infrequent pancreatic neoplasm in childhood, making up approximately 0.5% of pancreatic non-endocrine tumours. Typically, CMN is radiologically characterized by a solid cystic lesion with a uniform appearance, while PBL is signified by rapid cystic expansion with distinct boundaries, delineation, and heterogeneous density, often accompanied by necrotic areas, calcifications, and variable contrast enhancement on imaging studies. The coexistence of both CMN and PBL in the same patient is an exceedingly rare occurrence, and there is a complete absence of reported cases in the medical literature. Subjects and Methods: A 27-day-old male infant had a left renal mass detected on prenatal ultrasound 3 days before birth. Upon admission, abdominal ultrasound and contrast-enhanced computed tomography (CT) were conducted, revealing an enlarged left kidney with an irregular isodensity soft tissue mass at the lower pole, and a round soft tissue mass shadow in the middle and tail of the pancreas. Following completion of all preoperative assessments, the infant underwent surgical resection. The tumor was then completely removed. The pathological examination suggests a possible diagnosis of congenital mesoblastic nephroma and pancreatoblastoma. The patient was subsequently cured and discharged, followed by regular outpatient follow-up examinations, and no tumor recurrence was detected for 1 year and 8 months.

Results

An abdominal ultrasound and abdominal contrast-enhanced computed tomography (CT) revealed a mass located at the lower pole of the renal hilum. This mass exhibited heterogeneous internal echoes and small cystic cavities, and was bounded by the remnants of the kidney along its outer edge. Upon contrast administration, the mass demonstrated obvious heterogeneous enhancement, with a prominent edge enhancement effect. The remnants of the kidney were observed surrounding the outer edge of the mass, and compression of the renal artery was noted. Additionally, the pancreas appeared deformed with the presence of a cystic-solid mixed mass, which had clear boundaries. The solid portion of the mass presented as lobulated, and dense granular calcification points were observed. Multiple cystic cavities were seen surrounding this solid portion. Furthermore, the splenic vein was found to be compressed and narrowed. Upon contrast enhancement, the mass exhibited obvious heterogeneous enhancement, with multiple patchy non-enhanced areas.

Conclusions

Infants less than 3 months old presenting with masses in both the kidney and pancreas require careful evaluation. If the imaging characteristics of the renal mass indicate a large, well-circumscribed lesion, while the pancreatic mass suggests a more aggressive tumor profile, it is crucial to entertain the diagnosis of congenital mesoblastic nephroma associated with pancreatoblastoma. BEPS24009: CLINICAL EFFICACY OF TRADITIONAL CHINESE MEDICINE IN TREATING DIABETIC RETINOPATHY: BLOOD ACTIVATION AND STASIS REMOVAL, AND MERIDIAN UNBLOCKING FOR VISION IMPROVEMENT Xiaoqing Denga, Lijuan Sunb aDepartment of Traditional Chinese Medicine Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

To explore the clinical research on Traditional Chinese Medicine (TCM) treatment for diabetic retinopathy using the principles of promoting blood circulation to remove blood stasis and clearing meridians to improve vision.

Methods

A total of 100 patients with diabetic retinopathy treated in the TCM Ophthalmology Department of Handan Eye Hospital from April 2022 to April 2023 were selected as subjects. According to the principle of random allocation, both the treatment group and the control group consisted of 50 cases each. Both groups received basic treatment plans including blood sugar reduction and pressure control, while the treatment group additionally received traditional Chinese herbal decoction treatment based on the principles of promoting blood circulation to remove blood stasis and clearing meridians to improve vision. The control group was treated with Western medicine Dexamethasone Sodium Phosphate. The treatment duration was 2 months.

Results

The comprehensive efficacy, TCM symptoms, visual acuity, and macular retinal thickness in the treatment group were significantly better than those in the control group, with good clinical efficacy and no toxic side effects. The difference between the two groups was significant and statistically meaningful (P<0.05).

Conclusion

This study verified the effectiveness and safety of Traditional Chinese Medicine (TCM) characteristic therapy—the method of activating blood circulation to dissipate blood stasis and clearing channels to improve vision—in treating Diabetic Retinopathy (DR) through clinical experiments. This treatment not only significantly improved the comprehensive therapeutic effect, TCM syndromes, visual acuity, and retinal thickness in the macular area of patients but also did not observe any toxic side effects throughout the treatment process, indicating its high safety. Moreover, compared with the traditional Western medicine treatment using Dao Sheng Ming, this TCM theory-based approach can more comprehensively regulate the internal environment of patients, promote blood circulation, reduce inflammatory responses, thereby achieving the purpose of protecting visual function. BEPS24010: EFFECTS OF EXOGENOUS SPERMIDINE ON PHOTOSYNTHETIC CHARACTERISTICS AND CHLOROPLAST ULTRASTRUCTURE IN TOMATO SEEDLINGS UNDER HIGH TEMPERATURE STRESS WITHIN THE CONTEXT OF FOOD SAFETY AND NUTRIENT INTAKE Xiaoqiong Sua, Zhiwei Tanga aHunan Biological and Electromechanical Polytechnic, Changsha 410127, China.

Background

Temperature is the major environmental factor affecting plant normal growth. Tomato (Lycopersicon esculentum) is thermophilic but cannot tolerate high temperature. In recent years, more and more studies have been conducted on the use of exogenous polyamines to regulate stress tolerance in plants. However, studies on photosynthetic physiology of vegetable crops regulated by polyamines under high temperature stress are rare. Polyamines mainly include divalent putrescine (Put), trivalent spermidine (Spd) and tetravalent spermidine (Spm). The application of exogenous spermidine in improving the tolerance of tomato seedlings to adverse conditions not only has significant implications for agricultural production, but is also closely related to human food safety, nutrient intake, psychological health, and physical health. By applying this biotechnology, we can better ensure the quality and supply of food, while promoting the physical and mental health of people. Subjects and Methods: The effects of exogenous spermidine on the structure and function of photosynthetic organs of tomato leaves under high temperature stress (38 °C/28 °C, day/night) were studied by spraying spermidine on the leaves of ‘Puhong 968’, a heat-sensitive tomato cultivar, in an artificial climate chamber. The mechanism of exogenous Spd regulating the structure and function of photosynthetic organs in tomato seedlings under high temperature stress was investigated from the aspects of photosynthesis, ultrastructure of chloroplast and lipid composition of chloroplast membrane. This research is particularly relevant to maintaining the nutritional quality of food crops, which is essential for both physical health and the prevention of malnutrition-related diseases.

Results

The result showed that high temperature (38°C/28°C, day/night) stress for 7 days resulted in a significant inhibition of net photosynthesis (Pn) and the maximal photochemical efficiency of photosystem II (Fv/Fm). Application of exogenous 1mM Spd alleviated the reduction of Pn and Fv/Fm under high temperature conditions. High temperature stress (38 °C/28 °C, day/night, 7 days) reduced the thylakoid membrane lipid unsaturated, in which the content of palmitic acid (C16:0) increased significantly while the content of stearic acid (C18:0) and linolenic acid (C18:3) decreased significantly. The microscopic analysis revealed that high temperature stress induced destruction of chloroplasts with ruptured thylakoid membrane. However, Spd increased the level of lipid unsaturation in the chloroplast membranes and prevented the destruction of photosynthetic apparatus induced by high temperature stress. By improving the stress resistance of tomato seedlings, tomatoes retain their nutritional value even in times of adversity, thus helping people to get the necessary nutrient intake.

Conclusions

Exogenous spermidine treatment can improve photochemical activity of PSⅡ mainly by maintaining the integrity of chloroplast membrane and the stability of photosynthetic organs, so as to effectively alleviate the inhibition of high temperature stress on photosynthetic activity of tomato seedlings. By improving the stress resistance of tomato seedlings, it can be ensured that tomatoes retain their nutritional value even in times of adversity, thus helping people to get the necessary nutrient intake. At the same time, studying how plants adapt to heat stress can help understanding the potential impacts of extreme climate conditions on human health and provide a scientific basis for public health interventions. Acknowledgments: This work was funded and sponsored by Hunan Provincial Education Science Planning Project (Y20230574). BEPS24011: DETERMINATION OF RESIDUES OF FLUROXYPYR, NICOSULFURON AND ATRAZINE IN SOIL BY ASE-HPLC-MS/MS Jian ling Bia,b,c, Yang jian Gua,b,c, Peng fei Suna,b,c, Chun li Xua,b,c, Lu Chena,b,c, You gang Wangd, Xing lei Tiane aShandong Institute of Physical and Chemical Exploration, Jinan, Shandong 250013, China,bShandong Geological Exploration Engineering Technology Research Center, Jinan, Shandong 250013, China,cShandong Engineering Research Center for High Precision Detection of Underground Resources and Environment, Jinan, Shandong 250013, China,dHangzhou Best Gas Co., Ltd., Hangzhou, Zhejiang 311404, China,eShandong Institute of Geological Sciences, Jinan, Shandong 250013, China.

Background

A method for detecting residues of fluroxypyr, nicosulfuron, and atrazine in soil was established using accelerated solvent extraction (ASE) and graphitized carbon black (GCB) cleanup columns for sample pretreatment, followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Freeze-dried soil samples were extracted with 2% methanol-acetonitrile mixed solvent, concentrated under vacuum, dissolved in 1.0 mL methanol, and purified with GCB cleanup columns (containing 1 g of anhydrous magnesium sulfate). Separation was performed using an Acquity UPLC BEH C18 column (50 mm * 2.1 mm, 1.7 μm), and analysis was carried out in multiple reaction monitoring (MRM) positive ion mode with external standard quantification. The results showed good linear relationships for fluroxypyr, nicosulfuron, and atrazine in the concentration range of 0.50-500 μg/L, with correlation coefficients (R) all >0.99. With a sample size of 10.0 g, the detection limits were 0.5 μg/kg, 0.4 μg/kg, and 0.2 μg/kg, respectively, with spiked recovery rates of 83.7%-90.9% and relative standard deviations (RSD) of 1.42%-5.67%. This method is simple, accurate, and sensitive, suitable for the determination of fluroxypyr, nicosulfuron, and atrazine residues in soil. To explore the determination methods applicable to the residues of fluroxypyr, nicosulfuron, and atrazine in soil, and to summarize the application experience. Subjects and Methods: To explore the determination methods applicable to the residues of fluroxypyr, nicosulfuron, and atrazine in soil, the suitable sample extraction solvent, sample extraction temperature, purification column, linearity, accuracy, and precision of this method were studied. Actual soil samples were collected from agricultural land in Dezhou City were used for testing to verify the applicability of this method.

Results

The suitable extraction solvent is acetonitrile containing 2% formic acid, and the suitable extraction temperature is 90 °C. GCB column is selected as the sample purification column, which has a good purification effect on soil samples, the recovery rate of matrix addition and the results of parallel sample determination meet the testing requirements. The mass concentration linear ranges for fluroxypyr, nicosulfuron, and atrazine were 0.50-500 μg/L, with correlation coefficients (r²) ranging from 0.9973 to 0.9990. A good linear relationship was observed between the content of the target substances and their corresponding peak areas. The limits of detection (LODs) for fluroxypyr, nicosulfuron, and atrazine were 0.6 μg/kg, 0.4 μg/kg, and 0.2 μg/kg, respectively, and the limits of quantification (LOQs) were 1.9 μg/kg, 1.4 μg/kg, and 0.7 μg/kg, respectively. The recovery rates for the three target substances ranged from 83.7% to 90.9% across the three different spiked levels, with RSDs ranging from 1.42% to 5.67%, meeting the analytical testing requirements. Actual Sample Testing indicated that this method was suitable for the determination of fluroxypyr, nicosulfuron, and atrazine residues in soil samples.

Conclusions

This study established a method based on pressurized fluid extraction, graphitized carbon black cleanup columns, and ultra-performance liquid chromatography-tandem quadrupole mass spectrometry for the detection of fluroxypyr, nicosulfuron, and atrazine residues in soil. The method has low detection limits, good accuracy and precision, and a simple and efficient sample processing procedure. It is suitable for the determination of fluroxypyr, nicosulfuron, and atrazine residues in soil.

Acknowledgements

This work was supported by the Project of Natural Science Foundation of Shandong province (Grant No. ZR2022MD001). and 2023 technology project of Shandong Institute of Geophysical and Geochemical Exploration (Grant No. KY202307). BEPS24012: SIMULATION MECHANICAL ANALYSIS OF HUMAN FEMORAL BONE GRAFTING BASED ON DYNAMIC MECHANICS Kaixin Lina, Kaihuan Yua, Ning Lia,b, Jiahuan Zhouc aCollege of Engineering of Zhejiang Normal University, Jinhua 321000, China,bXingZhi College, Zhejiang Normal University, Jinhua, 321000; China,cYuyao Hospital of Traditional Chinese Medicine. Ningbo, 315000, China.

Objective

The natural biological properties of bone structure will endow biomimetic bone with biomechanical properties similar to natural bone. Gait walking in daily human life is a complex process of muscle mechanics. Studying the mechanical performance of internal connection methods in patients after bone grafting surgery under gait conditions is of significant importance. The researches on the dynamic mechanical properties and stress shielding effects of biomimetic bone can provide a preventive basis for the implementation plan of biomimetic bone implantation, and also provide solutions for the rejection behavior of implants. Subjects and Methods: An effective three-dimensional model of bone structure and knee joint were constructed by using finite element technology, and the biomimetic characteristics of these structure was analyzed. To compare the differences in gait mechanics after orthopedic surgery, this research obtained muscle force data through inverse dynamics calculations using the Anybody Modeling System and conducted joint mechanical simulations using Abaqus software.

Results

The results showed that during the stance phase of gait, the midsection of the femur experienced the highest stress, with the maximum Mises stress concentrated between the femoral trochanter and the femoral shaft, peaking at 23.875 MPa. This stress distribution is consistent with the pathological results of femoral fractures. Additionally, gait simulation analysis was performed on several common types of fractures to compare different bone connection methods. The intramedullary nailing technique showed a more favorable stress distribution for treating mid-femoral fractures compared to the plate technique. For femoral neck fractures, the anti-rotation intramedullary nail demonstrated superior load-bearing capacity compared to total hip arthroplasty.

Conclusions

This research provides a comparative analysis of femoral implants under gait motion using inverse dynamics and finite element analysis, offering a simulation reference for the selection of orthopedic surgical implants. Comparing the simulation results of bone repair after operation, the maximum stress of anti-rotation intramedullary nail in femoral neck fracture is less than that of artificial hip replacement, and intramedullary nail can effectively control the axial force of fracture part, prevent bone rotation and avoid stress shielding. In the fracture of the middle femur, the intramedullary nail repair technology has stronger strain resistance than the bone plate repair technology, which can effectively avoid the strain possibility under the shear stress of the middle femur. Acknowledgments: The project is supported by Natural Science Foundation of Zhejiang Province of China (Grant No. LY22E050001); State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (LSL-2113); Public Welfare Technology Application Research Project of Jinhua (2023-4-006); Major/Key research plan of the Jinhua Science and Technology Agency (2021-1-075). BEPS24013: EFFECT OF TAI CHI ON PSYCHOLOGICAL SYMPTOMS AND FRAILTY IN PATIENTS WITH CEREBRAL SMALL VESSEL DISEASE Ying Guoa, Kailin Gongb, Shengyi Zhanga, Lili Zhanga aDepartment of Neurology, Jiangnan University Affiliated Hospital, Wuxi, China,bDepartment of Physical Diagnosis, Affiliated Brain Hospital of Nanjing Medical University, Nanjing, China.

Background

and Purpose: Cerebral small vessel disease (CSVD) is a common cause of psychological distress, and physical frailty in older adults. Tai Chi, a traditional Chinese mind-body exercise, has shown promise in enhancing both psychological and physical well-being in various populations. However, its effects on CSVD patients remain largely unknown. This study aims to explore the impact of Tai Chi on psychological symptoms and frailty in these CSVD patients.

Methods

Patients were recruited from the Department of Neurology from August 2021 to August 2023. The participants were randomly assigned to the Tai Chi intervention or control group using a computer-generated random number sequence, stratified by age, gender, and education level. The allocation was concealed from researchers and participants until group assignment. Evaluation criteria: Data was collected before treatment and 12 weeks after. The primary evaluation indicators include mental health, frailty status, cognition, and quality of sleep. Tai Chi Group: Tai Chi Group: Participants in the Tai Chi group were invited to engage in a structured Tai Chi program led by certified Tai Chi instructors. Sessions occurred twice weekly, each lasting 60 minutes, spanning a 12-week period. This Tai Chi program centered on the Yang style, emphasizing slow, mindful, and continuous movements accompanied by deep breathing. Participants were encouraged to practice at home in between sessions. Control Group: Those patients in the control group maintained their regular routines and activities, without receiving any specific Tai Chi training or instruction. However, they were given the opportunity to join the Tai Chi program upon the study’s conclusion.

Results

After 12 weeks, the Tai Chi group(n=138) showed a significant reduction in anxiety scores compared to baseline (P<0.001), while there was no significant change in depression scores between the groups. Regarding frailty, after 12 weeks, the Tai Chi group had a significantly lower frailty scores compared to the control group (n=128) (P<0.001).

Conclusion

Our study suggests Tai Chi intervention can effectively reduce anxiety and improve frailty status in CSVD patients. Tai Chi appears to be a promising non-pharmacological approach for managing psychological and physical symptoms in CSVD patients. BEPS24014: GLOBAL TRENDS AND FUTURE DIRECTIONS IN END-OF-LIFE CARE: A BIBLIOMETRIC ANALYSIS FROM A MEDICAL PERSPECTIVE Caixia Liua, Yuan Liangb, Hong Changc, Li Jiad,e, Yajing Wuf aSwan College, Central South University of Forestry and Technology, Changsha 410004, China,bDongguan Nancheng Hospital, Dongguan 523715, China,cSchool of Marxism, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China,dUniversal Quality Education Institute, Wuchang University of Technology, Wuhan 430223, China,eCollege of Humanities and Management, Guangdong Medical University, Dongguan 523808, China,fSchool of Sociology and Public Administration, Guangdong University of Foreign Studies, Guangzhou 510225, China. CL and YL contributed equally to this study.

Background

End-of-life care research has gained significant attention in recent years, addressing the medical, emotional, and social needs of terminal patients and their families. This research investigates the evolving healthcare models, emphasizing the role of interdisciplinary collaboration and the integration of digital technologies in enhancing end-of-life care delivery. Subjects and Methods: This study utilized bibliometric analysis to explore global research trends in end-of-life care, drawing on data from the Web of Science. CiteSpace software was employed to construct a scientific knowledge map that highlights key themes, emerging research areas, and collaboration networks. The analysis provides a comprehensive overview of the field’s evolution, identifying influential publications and forecasting future trends based on citation patterns and thematic shifts.

Results

The analysis reveals a marked increase in research activity related to end-of-life care, characterized by a surge in publications and a growing number of international collaborations. Major research areas include palliative care, hospice services, and personalized care plans for terminal patients, particularly those with cancer. Emerging trends emphasize attitudes towards end-of-life care, patient experiences, and the impact of events such as the COVID-19 pandemic. Prominent keywords like “palliative care,” “hospice,” and “cancer” reflect established research areas, while terms such as “attitude,” “experience,” and “COVID-19” highlight emerging concerns. The Structural Variation Model was used to predict the influence of forthcoming publications in the field, revealing an increasing focus on personalized and patient-centered care strategies.

Conclusions

The findings underscore the growing importance of interdisciplinary collaboration in end-of-life care, integrating medical, technological, social, and psychological perspectives to enhance care models. Digital technologies, including telemedicine, artificial intelligence, and big data, are identified as key drivers of innovation, enabling more personalized, accessible, and efficient care delivery. These technologies facilitate improved decision-making processes and ensure that care plans are tailored to the unique needs of patients and their families. Furthermore, advancements in wearable health devices and biotechnology offer promising opportunities for real-time health monitoring and targeted interventions. The study concludes that a holistic, patient-centered approach is crucial for improving the quality of life for terminal patients, while also providing comprehensive support for caregivers and healthcare providers. This research highlights the importance of ongoing advancements and collaborative efforts in shaping the future of end-of-life care.

Acknowledgements

This work was supported by a project grant from “Human Enhancement Technology Risk Research: Causes, Models and Protocols”, Ministry of Education, Humanities and Social Sciences Research Youth Project (22YJCZH012). BEPS24015: EFFECTS OF DUAL COPING MODEL ON QUALITY OF LIFE AND PSYCHOLOGICAL RESILIENCE OF PRIMARY CAREGIVERS OF PATIENTS WITH SPECIFIC DERMATITIS Yanjun Zhaoa, Danfeng Chub, Rong Zhangc aDermatology, The 989th Hospital of the People’s Liberation Army, Pingdingshan 467000, Henan, China,bDepartment of Dermatology, Chang hai Hospital of Naval Medical University, Shanghai 200433, China,cNursing Department, The 989th Hospital of the People’s Liberation Army, Luoyang 471000, Henan, China.

Background

To study the effect of dual coping model on the quality of life of patients with specific dermatitis and the psychological resilience of the main caregivers, Developing specific dichotomous interventions to explore effective ways to improve the quality of life of people with atopic dermatitis and the psychological resilience of primary caregivers. Subjects and Methods: From February 2021 to February 2022, 120 pairs of patients with specific dermatitis and their main caregivers hospitalized from the dermatology of Chang hai hospital were randomly divided into a control group and an intervention group, with 60 pairs in each group Patients in the control group were given routine dermatologic care during the treatment period and their caregivers were given routine counseling; patients in the study group were given a binary coping model intervention based on routine dermatologic care for patients and their caregivers, establishment of dichotomous intervention teams, develop binary interventions for the first week, the second week, the third week of intervention and post-discharge in accordance with the nursing clinical pathway.

Results

Before the intervention, the difference in quality of life scores between the two groups of atopic dermatitis patients was not statistically significant (P>0.05); after the intervention from the first and the second weeks to the first month after discharge, the difference in quality of life scores between the two groups of atopic dermatitis patients was significant (P0.05); the first week after the intervention, the difference between the two groups of The difference in mental toughness scores of primary caregivers of atopic dermatitis patients was statistically significant (P<0.05); from the second week after intervention to the first month after discharge, the difference in mental toughness scores of primary caregivers of atopic dermatitis patients between the two groups was significant (P<0.01).

Conclusions

Dualistic coping model intervention improves quality of life for patients with atopic dermatitis and enhances psychological resilience of the primary caregiver, and the Dualistic Coping Model Intervention deserves to be referenced and recommended in the clinic. BEPS24016: TREATMENT AND NURSING CARE OF AN ELDERLY PATIENT WITH SEVERE TETANUS WITHOUT DEFINITE HISTORY OF TRAUMA Yongjuan Fenga, Wei Zhanga, Di Zhanga aGuang’an People’s Hospital, Guang’an 638000, Sichuan, China.

Background

Tetanus is a serious infectious disease, which often occurs after trauma. However, some patients do not have a clear history of trauma. Tetanus has characterized by an acute onset, rapid progression and poor prognosis. Its pathological mechanism is mainly related to the invasion of the body by Clostridium tetani and the release of neurotoxins. Studies have shown that tetanus vaccination is one of the key factors in disease control, but high-quality care is also needed during treatment. Subjects and Methods: A case of severe tetanus in an elderly patient with no definite history of trauma was reported. The patient was desensitized and received Tetanus vaccine and comprehensive care. In the course of treatment, medical staff pay close attention to the physiological and psychological conditions of patients, and provide corresponding health education and nursing to meet the actual needs of the body in all aspects, and improve the compliance of patients with treatment and nursing.

Results

Through Tetanus vaccine and integrated care services, the patient’s condition was controlled and relieved. The nursing team provided a personalized care plan that included regular wound cleaning and replacement, nutritional support, respiratory management, pain control, and more. The patients’ muscle spasms were relieved and their daily life functions were improved.

Conclusions

For the elder patients with severe tetanus without a definite history of trauma, Tetanus vaccine injection is an important treatment. In addition, comprehensive care services also play an important role in improving the prognosis of patients. With an individualized care plan, comprehensive support and assistance can be provided to facilitate patient recovery. This study provides useful experience for clinical practice and emphasizes the importance of nursing in the treatment of tetanus. BEPS24017: COMPETITIVE INTERACTION OF HUMAN SERUM PROTEINS WITH LOPINAVIR AND RITONAVIR Yongchun Liua, Fei Lib, Yijie Hea, Jing Luoc, Siyuan Wangc, Zixuan Liangc, Huili Qia, Xiaoxia Weia, Chunxiao Hea, Yingying Lia, Ruixia Leia, Yingshuang Shia, Mengyuan Yina aCollege of Petroleum and Chemical Engineering, FLUOBON Pilot Base of Chemical Engineering, Longdong University, Qingyang, Gansu 745000, China,bCentral Blood Station of Qingyang City, Qingyang, Gansu 745000, China,cCenter for Disease Control And Prevention of Qingyang City, Qingyang, Gansu 745000, China.

Background

The effectiveness and activity of drugs are multifaceted and complex. The binding of serum proteins to drug affects its transport, diffusion, distribution at the lesion site and blood in vivo. This study is aimed to investigate the interactional properties and the competitive relationships between lopinavir and ritonavir with main human serum proteins under simulated human physiological conditions in vitro. Subjects and Methods: Fluorescence titration was used to investigate the binding constants (Ko), the quenching rate constants of bimolecular diffusion collision (Kq), the Stern-Volmer dynamic quenching constants (KSV), the thermodynamic parameters. The BIOVIA Discovery Studio 2019 and Pymol software were used to perform molecular docking. Centrifugal ultrafiltration and Ultraviolet-visible (UV-vis) spectrometry were used to calculate the molar binding rate of serum proteins and drug in plasma.

Results

Molecular docking results indicate the receptor-ligand interactions are stronger except that both lopinavir and ritonavir can’t bind to CDR of Fab fragment of IgG and 3GHG (γ) of Fibrinogen, that is, both lopinavir and ritonavir interact nonspecific with the Fab fragment of IgG. The ratio of Ko for IVIg: HSA: Fibrinogen binding to lopinavir is maintained at 1.0: 1.50~1.96: 2.05~0.11, and the ratio of Ko for IVIg: HSA: Fibrinogen binding to ritonavir is maintained at 1.0: 2.79~0.17: 0.30~0.11 when the binding site is taken as 1~4. If the influence of the other serum proteins and factors on drug binding is ignored, and only one binding site of proteins to drug is considered, then only 1/40.54 of mole fraction of lopinavir or only 1/36.56 of mole fraction of ritonavir can be effectively bound by IgG and targeted for transport to the lesion site, while 0.29 mole fraction of lopinavir or 0.60 mole fraction of ritonavir can be effectively bound by HSA and transported to cell surfaces. When the molar ratios of drug to protein change from 1:1 to 6:1, the average molar binding rates (BR%) of proteins to lopinavir and ritonavir in plasma are 58.60±5.00% and 95.17±3.23%, respectively, and the BR% of proteins to drugs are not concentration-dependence relationships. With the decrease of pH, all the bindings of three proteins to lopinavir and ritonavir show gradually decreasing trends, especially the bindings of HSA to ritonavir and Fibrinogen to lopinavir show sharply decreases after pH 6.80.

Conclusions

There are competitively interactional relationships between main human serum proteins and lopinavir or ritonavir. Due to high binding abilities of proteins to drugs, the concentration of free drugs in the blood will be lower, and the concentration of drugs that can freely or passively diffuse into cells will be much lower. In future clinical application, a lotion with drug combination stoichiometric ratio of 4~6:1 of drug: IVIg can be recommended and prepared for intravenous injection. Combination therapy of high-dose IVIg and drugs will target and transport to the lesion site, leading to more internalization of antibodies into the lesion and further improving the efficacy of the drug.

Acknowledgements

This study was supported from the Natural Science Foundation of Gansu (No. 21JR7RM187), Science and Technology Commissioner Special Project of Gansu province (No. 22CX8GM138) and Youth Doctoral Project of Gansu province (No. 2023QB-016). In addition, we are thankful to Mr. Yanfeng Li and Beijing Zhongke Ruiyun Technology Co., Ltd for providing the Molecular Docking calculations. BEPS24018: APPLICATION RESEARCH OF INTEGRATED CHINESE AND WESTERN MEDICINE IN THE TREATMENT OF ISCHEMIC OPTIC NEUROPATHY IN THE ELDERLY POPULATION Hongyu Sua, Lijuan Sunb aDepartment of Ophthalmological Internal Medicine, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

Study on the Application Effect of Integrated Chinese and Western Medicine in Treating Ischemic Optic Neuropathy in Elderly Populations.

Methods

A total of 108 elderly patients with ischemic optic neuropathy who visited the ophthalmology outpatient clinic or were hospitalized at our hospital were selected as research subjects for analysis. The 108 patients were randomly divided into two groups: a treatment group of 57 cases (60 eyes) treated with integrated Chinese and Western medicine, and a control group of 51 cases (60 eyes) treated with conventional Western medicine. The clinical application effects (therapeutic efficacy, visual acuity index, average sensitivity of visual field, average defect of visual field) and adverse reactions were compared between the two groups.

Results

The overall effective rate of the treatment group was 85.00%, higher than the control group’s 68.33%, with a statistically significant difference (χ²=4.66, P=0.031). After treatment, both groups showed improved visual acuity index and average sensitivity of the visual field compared to before treatment, and the average defect of the visual field decreased compared to before treatment. The changes in the treatment group were greater than those in the control group, with all differences being statistically significant (P < 0.05). No adverse reactions occurred in either the treatment group or the control group.

Conclusion

This study demonstrates that, compared to conventional Western pharmaceutical treatment alone, the integration of Traditional Chinese Medicine (TCM) and Western medicine exhibits significant clinical advantages in treating elderly patients with ischemic optic neuropathy. Specifically, the overall effectiveness rate for the treatment group reached 85.00%, significantly higher than the 68.33% observed in the control group. This indicates that the combined approach more effectively improves patient symptoms and visual acuity. Moreover, post-treatment, the treatment group exhibited higher visual acuity indexes and mean sensitivity within the visual field, while showing lower mean defects in the visual field compared to pre-treatment levels. These changes were more pronounced than those in the control group, further confirming the efficacy of integrative treatment in enhancing visual quality and improving visual function. Notably, no adverse reactions occurred in either the treatment or control groups throughout the treatment period, demonstrating the safety of the combined TCM and Western medicine approach without additional risks. In summary, the combination of TCM and Western medicine not only effectively increases the cure rate and quality of life for elderly patients with ischemic optic neuropathy but is also safe and reliable. It represents a new therapy worth promoting and applying. By integrating the holistic regulation of TCM with the targeted treatment of Western medicine, a more comprehensive and personalized treatment plan can be provided for patients, better meeting their diverse needs and promoting their recovery process. BEPS24019: ENDOSCOPIC TREATMENT OF ANTERIOR CLINOID MUCOCELE WITH COMPRESSIVE OPTIC NEUROPATHY Guidong Songa, Yong Xua, Hao Donga, Yong Lia, Ying Zhub, Jun Kanga aDepartment of Neurosurgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China,bThe Fourteenth Retired Veteran Cadre’s Sanatorium of Fengtai District, Beijing, China.

Background

Mucocele of the anterior clinoid is extremely rare, accounting for about 1% of paranasal sinus mucoceles. Misdiagnosis and mistreatment may occur in clinical practice, which may delay the treatment and cause irreversible visual damage, even blindness. To analyzed the clinical manifestations and characteristics and investigate the outcomes of endoscopic treatment of anterior clinoid mucocele with optic neuropathy. Subjects and Methods: A retrospective analysis was made on the patients with anterior clinical process mucocele with compressive optic neuropathy who were treated by endoscopic endonasal approach from 2016 to April 2024 in the department of Neurosurgery, Beijing Tongren Hospital, Capital University. All patients had visual loss and/or visual field defects before surgery. High-resolution CT and MRI were performed in all patients. The masses were located in the anterior clinoid process and proved to be mucoceles by postoperative pathology. The preoperative visual function was evaluated and the postoperative visual function was followed up.

Results

A total of 5 patients were included in this study, including 3 males and 2 females, aged 32~66 years old, with an average age of 53 years. The most common symptoms were vision loss, which occurred in all of the 5 cases. The time from onset to surgery was 18 to 40 days. All of the 5 patients underwent endoscopic endonasal trans-ethmoidal anterior clinoid mucocele resection and optic nerve decompression. The postoperative visual function was improved in 3 cases, and 2 patients stayed unchanged. The 4 patients with no light perception before surgery, 2 patients regained vision, and 2 patients stayed unchanged after surgery. No postoperative complications such as cerebrospinal fluid leakage and infection occurred.

Conclusions

Anterior clinoid mucoceles have an extremely low incidence and are prone to misdiagnosis and mistreatment during clinical practice, leading to irreversible vision damage. Endoscopic treatment is an effective method to treat the anterior clinoid mucocele compressive optic neuropathy. Early diagnosis and surgical excision can effectively restore the visual function of the patients. BEPS24020: SA-SDN: SYMPTOM DISENTANGLEMENT NETWORK FOR SYNDROME-AWARE HERB RECOMMENDATION Chaoyang Lia, Haobo Lib, Pengtao Jiab, Kegang Cuic, Yanjin Lic aDepartment of Proctology, Xi’an Hospital of Traditional Chinese Medicine, Xi’an 710021, China,bCollege of Computer Science and Technology, Xi’an University of Science and Technology, Xi’an 710054, China,cDepartment of Information Technology, Xi’an Hospital of Traditional Chinese Medicine, Xi’an 710021, China.

Background

Chinese herbal prescriptions represent the long-term practices of traditional Chinese medicine (TCM), embodying its unique therapeutic methods and clinical experiences. Therefore, leveraging data mining techniques and a substantial amount of empirical prescriptions to construct an herb recommendation model tailored to specific diseases holds significant importance for the transmission of TCM knowledge. However, the existing herbal medicine recommendation methods are often limited to modeling the relationships between diseases and herbs, failing to sufficiently reflect the diagnostic and therapeutic principles of TCM, particularly the differentiation among syndromes.

Methods

In this paper, we delve into the potential mechanisms of symptoms in the syndrome differentiation and reasoning process and propose a symptom disentanglement network for a syndrome-aware herb recommendation model (SA-SDN). First, a graph neural network (GNN) is employed to generate entity representations in a disease-prescription bipartite graph and a syndrome-herb bipartite graph, and by constructing a herb knowledge graph, we introduce the properties of herbs as additional auxiliary information. Subsequently, a symptom disentanglement model is introduced to extract latent symptom representations from disease embeddings. Building upon this, we propose a hybrid attention layer that combines symptom aggregation attention and sequence fusion attention to deduce the final syndrome representations based on different symptoms. Finally, the syndrome representations are interacted with the herb representations to produce herb recommendations.

Results

Extensive experiments conducted on a real clinical dataset demonstrate that the SA-SDN achieves average Precision@10, Recall@10, and NDCG@10 improvements of 4.36%, 4.29%, and 2.98%, respectively, over the existing state-of-the-art baseline methods.

Conclusions

This further validates the effectiveness of the proposed model. These findings can provide a basis for assisting with the TCM clinical prescription process.

Acknowledgements

This research was funded by the Technology Innovation Leading Program of Shaanxi (Program No.2022QFY01-11) and Scientific and Technology Program Funded by Xi’an City (Program No. 22YXYJ0009). BEPS24021: CLINICAL EFFICACY ANALYSIS OF EAR EXERCISES FOR PATIENTS WITH TINNITUS ACCOMPANIED BY HEARING LOSS Zhenzhen Lia,#, YuanYuan Chena,#, Yuanxian Liua, Xiang Zoua, Mingming Xua aShenzhen Traditional Chinese Medicine Hospital, Shenzhen, China. #ZL and YC contributed equally to this study.

Objective

This study aims to explore the effectiveness of self-designed ear exercises, based on theories of organ meridians and channels, in alleviating tinnitus and associated hearing loss in patients. The primary objective is to determine whether the incorporation of these exercises can lead to significant improvements in the quality of life and auditory function in individuals suffering from these conditions. Subjects and Methods: Patients with tinnitus and hearing loss who visited a tertiary hospital in Shenzhen from January 2023 to August 2023 were selected as the study subjects. A total of 82 patients were randomly assigned to either a control group or an experimental group using a random number table, with 41 patients in each group. The control group received routine nursing intervention, while the experimental group received the same standard care plus the self-designed ear exercises. The intervention lasted for three months, with data collected before and after the intervention. Comparisons were made between the Tinnitus Handicap Inventory scores and pure tone audiometry thresholds before and after the intervention.

Results

Post-intervention, the total score of the Tinnitus Handicap Inventory in the experimental group (33.81±6.68) was significantly lower than that of the control group (35.67±7.63), showing a statistically significant difference (t1=2.096, P1=0.039). The pure tone audiometry results in the experimental group at 500Hz (20.54±8.12 dB), 1,000Hz (23.14±8.51 dB), and 2,000Hz (26.47±9.52 dB) were significantly better compared to the control group results of 500Hz (25.63±11.09 dB), 1000Hz (28.33±10.81 dB), and 2000Hz (31.81±11.12 dB), with statistical significance (t1=2.191, P1=0.032; t2=2.213, P2=0.029; t3=2.158, P3=0.034). There was no significant difference in the average hearing threshold at 4,000Hz between the groups (t1=0.180, P1=0.858).

Conclusion

The results of this study suggest that ear exercises are significantly effective in improving clinical outcomes for patients with tinnitus accompanied by hearing loss. The method is simple to operate, easy for patients to master, and feasible for widespread clinical implementation. This study provides evidence supporting the integration of traditional ear exercises into modern healthcare practices for the management of tinnitus and hearing loss, offering a non-invasive and culturally rooted alternative for patients seeking relief from these conditions. BEPS24022: QUESTIONNAIRE PREPARATION AND VALIDITY TEST OF UNDERGRADUATE WILLINGNESS TO INTERVENE IN PSYCHOLOGICAL CRISIS Rong Wanga, Hong Qiub, Qinsi Wua, Min Liua aHealth and Medical College, Nantong Institute of Technology, Nantong 226001, Jiangsu, China,bJilin Institute of Socialism, Changchun 130000, Jilin, China. RW and HQ contributed equally to this study.

Background

The development of a questionnaire assessing college students’ willingness to intervene in psychological crises and the testing of its reliability and validity provide a theoretical and practical foundation for the prevention and intervention of psychological crises in higher education institutions. Subjects and Methods: College students’ readiness to intervene in psychological crises was initially assessed using a questionnaire based on literature review, expert consultation, and field interviews. This study uses a random stratified sampling questionnaire survey approach, with 780 undergraduate students in Jiangsu Province serving as the study population. The questionnaire’s validity and reliability are examined, its questions are examined, and an exploratory factor analysis is done using the data from the empirical research.

Results

The questionnaire consists of four core factors: cognitive performance, emotional performance, behavioral performance and physiological performance, covering a total of 25 specific items. The cumulative contribution rate of variance is as high as 49.001%, which fully demonstrates the extensive coverage and in-depth analysis ability of the questionnaire. In terms of reliability, Cronbach’s α coefficient of the questionnaire reached 0.840, indicating the high stability and reliability of the questionnaire. Cronbach’s α coefficient of the four factors fluctuated between 0.693 and 0.856, which further proved that the questionnaire had good consistency in all dimensions. In terms of validity, the content validity ratio (CVR) of the questionnaire reached the full score standard, indicating that the questionnaire content was closely consistent with the actual research objectives. In addition, the correlation between the four factors ranged from 0.244 to 0.562 (P < 0.01), which not only indicated the relative independence of each factor, but also reflected the internal connection between them, which together constituted the complete framework of the questionnaire.

Conclusions

The questionnaire of college students’ willingness to intervene in psychological crisis is comprehensive in content and has good construction validity. The reliability and validity of the questionnaire are in line with the requirements of psychometrics, and can better reflect the current situation of undergraduate willingness to intervene in psychological crisis. It can be used for the investigation of the current situation of undergraduate psychological crisis intervention, as well as for the prevention, early warning and intervention of psychological crisis.

Acknowledgements

This paper was supported by a project grant from education science planning project of Jiangsu Province (Grant No. B/ 2022/ 01/151). This paper was supported by a project grant from undergraduate excellent graduation design (thesis) cultivation program project funding of Nantong Institute of Technology (Grant No.BS202453). This paper was supported by a project grant from 2023 Ministry of Education industry-university collaborative education project (Grant No. 231007653133509). This paper was supported by a project grant from The special project “Research on the Reform of High-quality Public Course Teaching” in Jiangsu Province in 2024(Grant No.2024GZJX098). This paper was supported by a project grant from key discipline construction project of Nantong Institute of Technology (Grant No.879005). This paper was supported by a project grant from Nantong Institute of Technology on-campus research project (Grant No.2022XK(J)32). BEPS24023: STUDY ON THE IMPACT OF MATERNAL MENTAL HEALTH STATUS ON INFANT SLEEP QUALITY AND ITS PREDICTION MODEL Yuan Longa,b, Ning Wanga,b, Ruijie Xiea, Jian Xionga aCollege of Artificial Intelligence,Guangzhou Huashang College, Guangzhou, China,bINTI International University, Putra Nilai, 71800 Negeri Sembilan, Malaysia.

Background

In recent years, with the increasing social attention to the healthy growth of infants and young children, the impact of mothers’ mental health on the growth of infants and young children has become an important research topic. In particular, mental health problems such as postpartum depression, anxiety and post-traumatic stress disorder (PTSD) after childbirth not only directly threaten the physical and mental health of mothers, but also can have a profound negative impact on the quality of sleep and overall growth of infants and young children by affecting the quality of mother-child interaction. In order to further explore this issue, this study focuses on the relationship between maternal mental health status and infant sleep quality, and attempts to establish a predictive model to provide scientific basis for public health intervention and clinical treatment. Subjects and Methods: Data were collected from 390 infants aged 3 to 12 months and their mothers, and the mothers’ mental health was comprehensively assessed using the CBTS (Child Birth-related Traumatic Stress), EPDS (Edinburgh Postnatal Depression Scale) and HADS (Health Anxiety and Depression Scale) scales. Based on these data, a stochastic forest classification prediction model and a BP neural network model optimized by genetic algorithm were established to analyze the complex relationship between mothers’ mental health and infants’ sleep quality, and the predictive ability of these models was verified.

Results

Mothers’ mental health problems significantly affected infants’ sleep quality, and the established prediction model showed high accuracy and reliability in both quantitative analysis and prediction.

Conclusions

This study not only provides a scientific basis for public health interventions, but also provides medical and mental health professionals with an effective predictive tool to support them to better assess and intervene in maternal mental health problems, and thus promote the healthy growth of infants and young children. Acknowledgments: This research is supported by the research on the development and application of Data mining technology in various industries in the Tutorial System project of Guangzhou Huashang University (Project No.: 2024HSDS14). BEPS24024: AN INTELLIGENT DISCRIMINANT MODEL OF COLORECTAL INFLAMMATION AND CANCER BASED ON MACHINE LEARNING Zhenguang Liua, Jianyun Wangb, Zhenghua Xub, Yaqi Yangb, Jiachen Xub, Qiaoqiao Liub aHealth School of Nuclear Industry, Affiliated Nanhua Hospital, University of South China, Hengyang 421001, Hunan, China,bSchool of Mathematics and Physics, University of South China, Hengyang 421001, Hunan, China.

Background

Colorectal cancer is a common malignant tumor of the digestive system. The incidence of colorectal cancer in China is increasing year by year, which has seriously endangered people’s health. However, there are few studies on how to identify colorectal inflammation and cancer only through colorectal biochemical indicators. The purpose of this study is to establish an intelligent statistical discriminant model of colorectal inflammation and cancer based on big data of medical records of colorectal-related diseases, which can achieve high-precision diagnosis of colorectal inflammation and cancer through routine examination results without pathological examination.

Methods

Case data came from a hospital in Hunan province, China. A total of 2883 samples were included in this study. After preprocessing the sample data, we used the ReliefF feature selection algorithm for feature extraction and obtained the score of each feature. Besides, we used the Pearson correlation coefficient and chi-square test to measure the relationship between two variables. Then we established a Bagging ensemble model based on Decision Tree Algorithm, Support Vector Machine Algorithm and Neural network Algorithm, which could discriminate colorectal inflammation and cancer. According to the result of Bagging ensemble model, in order to get a higher discriminant accuracy, the GentleBoost optimized ensemble model was established to identify colorectal inflammation and cancer through colorectal biochemical information.

Results

After feature selection and correlation testing, 26 indicators that affect colorectal cancer symptoms were finally screened out. Correlation test showed that they did not have much correlation with each other. The discriminant accuracy of the Bagging ensemble model was 98.5 %, and the discriminant accuracy of the GentleBoost optimized ensemble model was 99 %.

Conclusion

Our results have a significant effect in identifying inflammation and cancer in colorectal diseases, providing more accurate decision support for clinical practice and more possibilities for patients ‘ diagnosis and treatment. Meanwhile, we explored the importance of feature selection and how to combine medical expertise and mathematical perspectives to optimize the feature set. BEPS24025: COMPARATIVE ANALYSIS OF THE EFFECTS OF FEMTOSECOND LASER CATARACT SURGERY WITH TRADITIONAL PHACOEMULSIFICATION CATARACT SURGERY Yan Chena, Lijuan Sunb aDepartment of Ophthalmology, Linzhang Aiyan Ophthalmology Hospital, Handan, Hebei 056000, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan, Hebei 056000, China.

Background

With the continuous advancement of medical technology, cataract surgery methods are constantly being innovated and improved. Traditional phacoemulsification cataract surgery has been widely used for many years and has achieved significant therapeutic effects. However, in recent years, femtosecond laser-assisted cataract surgery (FLACS) as an emerging technology has gradually attracted the attention of ophthalmologists. Phacoemulsification cataract surgery is a procedure that uses high-frequency ultrasound to emulsify the cloudy lens nucleus and cortex, which are then suctioned out, followed by the implantation of an artificial lens. This method has the advantages of small incisions and quick recovery and is one of the mainstream techniques for cataract surgery currently. Femtosecond laser-assisted cataract surgery introduces femtosecond laser technology on top of the traditional surgery. The femtosecond laser can precisely cut the cornea, anterior lens capsule, and fragment the lens nucleus, thereby reducing mechanical damage to ocular tissues during the surgery, enhancing the precision and safety of the procedure. Additionally, the femtosecond laser allows for personalized corneal incision design, which helps optimize postoperative vision. This study investigates the differences in outcomes between these two surgical methods. To explore the application effect of femtosecond laser-assisted phacoemulsification technology in the operation of cataract patients.

Methods

A total of 100 patients (94 eyes) with secondary and tertiary nuclear cataracts who were admitted to the hospital from December 2023 to December 2024 were intercepted, and they were divided into study group and control group, with 50 cases (47 eyes) in each group, according to the different surgical methods they received. The femtosecond laser-assisted phacoemulsification cataract surgery was used in the study group, while the traditional phacoemulsification cataract surgery was used in the control group, and the anterior capsular incision diameter and capsule size of the subjects in the two groups were recorded and compared and analyzed, and the visual recovery, intraocular lens deviation rate and capsule centering were compared and observed in the two groups at 1 day, 1 month and 6 months after surgery.

Results

Compared with the control group, the anterior capsule incision diameter and capsule size were lower in the study group, and the difference was statistically significant (P0.05), while the incidence of intraocular lens deviation in the study group was significantly lower than that in the control group (P<0.05).

Conclusion

Femtosecond laser-assisted phacoemulsification cataract surgery has a high safety profile and outstanding clinical effect, which is suitable for widespread promotion and application in clinical practice. BEPS24026: CLINICAL CHARACTERISTICS ANALYSIS OF 294 CASES OF SEVERE LOWER RESPIRATORY TRACT INFECTION CAUSED BY hMPV Wei Suna, Jianwei Zhoub, Liang Zhoua aDepartment of Respiratory Four, Hebei Children’s Hospital, Shijiazhuang 050000, Hebei, China,bDepartment of Joint Orthopedics, Shijiazhuang People’s Hospital, Shijiazhuang 050000, Hebei, China.

Objective

To analyze the clinical characteristics of severe lower respiratory tract infections caused by human metapneumovirus (hMPV).

Methods

We collected cases of respiratory infections caused by hMPV treated in our department as research subjects and systematically gathered clinical data on patient age, infection timing, pathogen detection, clinical diagnosis, clinical manifestations, laboratory tests, risk factors for severe cases, treatment, and outcomes.

Results

Among 294 patients, there were 63 severe cases (21.43%) and 231 mild cases. hMPV infections occurred in 152 cases (51.70%) within the first 6 months, 32 cases (10.88%) from 6 to 12 months, 66 cases (22.45%) from 12 to 36 months, and 44 cases (14.97%) over 36 months. Of the 294 patients, 73 (24.83%) had co-infections with other viruses, and 85 (28.91%) had bacterial infections. The rates of acute tracheobronchitis, bronchitis, and pneumonia diagnoses were higher in the severe group than in the mild group. Among the 294 patients, wheezing was observed in 89 cases (30.27%), coughing in 231 cases (78.57%), fever in 92 cases (31.29%), expectoration in 37 cases (12.59%), inspiratory retractions in 127 cases (43.20%), audible wet rales in 132 cases (44.89%), wheezes in 208 cases (70.75%), and decreased breath sounds in 43 cases (14.63%). Complications included sepsis in 26 cases (8.84%), respiratory failure in 42 cases (14.29%), neutropenia in 25 cases (8.50%), liver damage in 17 cases (5.78%), and toxic encephalopathy in 9 cases (3.06%). The incidence of bilateral patchy consolidation, atelectasis, and pleural effusion on X-ray examinations was higher in the severe group than in the mild group. Severe group patients had higher levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), white blood cell count (WBC), lymphocyte percentage (LYM%), and monocyte count (MON#) compared to the mild group, while the neutrophil percentage (NEU%) and platelet count (PLT) were lower. Risk factors for severe hMPV-induced lower respiratory tract infections primarily included age <6 months, CRP ≥ 50 mg/L, malnutrition, and prematurity. After treatment with corticosteroids, interferon, antiviral drugs, antibiotics, nasal cannula oxygen therapy, intensive care, and mechanical ventilation, the course of illness ranged from 7 to 86 days (mean 63.25±25.41 days); 193 patients recovered (65.65%), 76 improved (25.85%), 25 remained uncured (8.50%), and there were no deaths. Both the severe and mild groups achieved good treatment outcomes.

Conclusion

Severe patients differed from mild patients in terms of age at infection, timing, pathogen infection situation, clinical diagnosis, clinical manifestations, laboratory test results, risk factor analysis for severe cases, treatment, and prognosis. Further increase in sample size is needed for statistical regression analysis to clarify the high-risk factors of critically ill patients. BEPS24027: A CASE OF RECURRENT URINARY TRACT INFECTION ACCOMPANIED BY ALTERED CONSCIOUSNESS: NEURONAL NUCLEAR INCLUSION BODY DISEASE AND LITERATURE REVIEW Guo Wena, Yong Penga aDepartment of Neurology, First Affiliated Hospital of Hunan University of Chinese Medicine, Zhuzhou 412000, Hunan, China.

Background

Neuronal intranuclear inclusion disease (NIID) is a rare neurodegenerative disorder characterized by the formation of eosinophilic transparent inclusions within the cell nucleus. NIID is uncommon in clinical practice and is often misdiagnosed or overlooked due to its complex and varied onset. The main clinical manifestations of NIID include cognitive impairment, episodic loss of consciousness, tremors, muscle weakness, behavioral abnormalities, bradykinesia, ataxia, and episodic encephalopathy. However, patients whose initial symptoms are recurrent urinary tract infections and neurogenic bladder symptoms, such as urinary incontinence, are often not considered to have NIID. Subjects and Methods: This study reports a case of a 70-year-old female patient with a 6-year history of recurrent episodic loss of consciousness, accompanied by urinary tract infections and urinary difficulties. A thorough analysis of the patient’s history, physical examination, auxiliary tests, skin biopsy, and genetic testing results was conducted. Initially, she presented with recurrent urinary tract infections and urinary difficulties, experiencing symptoms such as dizziness, palpitations, nausea, vomiting, and loss of consciousness. Later, she underwent bilateral stent placement due to ureteral obstruction. After the procedure, the patient had a long-term indwelling catheter and was hospitalized for treatment. Ultimately, due to repeated episodes of altered consciousness, a series of examinations, including head MRI, genetic testing, and skin biopsy, were performed.

Results

MRI of the patient’s head showed symmetrical abnormal signals in the bilateral frontal, temporal, and parietal lobes, displaying the characteristic “ribbon sign” at the cortical-subcortical junction. Genetic testing indicated that the GGC repeat count of the NOTCH2NLC gene exceeded the normal range, consistent with the pathogenic features of NIID. Skin biopsy results also demonstrated positive expression of eosinophilic inclusions in the nuclei of sweat gland duct epithelial cells. Furthermore, multiple urinalyses indicated urinary tract infections, and the patient exhibited symptoms of ureteral obstruction and urinary incontinence, ultimately diagnosed as neurogenic bladder. Based on these findings, the patient was confirmed to have NIID.

Conclusions

This article summarizes the clinical data and characteristics of this patient and reviews relevant literature, exploring the relationship between NIID and neurogenic bladder, as well as issues to consider in the diagnosis and treatment process. The results indicate that for patients presenting with recurrent urinary tract infections, urinary incontinence, and urinary retention, accompanied by frequent loss of consciousness and episodic encephalopathy, the possibility of NIID should be considered. Acknowledgments: This research is supported by the Key Guidance Project of Hunan Provincial Health Commission (C202303076574); Key Project of Hunan Provincial Administration of Traditional Chinese Medicine (A2023039); Project of Zhuzhou Science and Technology Bureau (2021-009); Excellent Scientific Research and Innovation Team of First Affiliated Hospital of Hunan University of Chinese Medicine (B2021-003); 2022 Joint Fund Project of Hunan University of Chinese Medicine (2022-44). BEPS24028: DEVELOPMENT AND RESEARCH ON THE MEDICINAL VALUE ABOUT GENETICALLY IMPROVED PHYSALIS Bo Wanga, Qiong Yanb aSchool of Biology and Agriculture, Jiamusi University, Jiamusi City 154007, Jiamusi,bBeijing Jiaotong University, Beijing 100044, China.

Background

Physalis Calyx Seu Fructus (PCF) is a natural wild herbal medicine that contains components with anti-inflammatory, anti-tumor, and immunomodulatory effects in pharmacology. Genetic transformation technology is an effective method for analyzing the genetic mechanisms of the synthesis and accumulation of secondary metabolites in medicinal plants. It plays a significant role in the research and development of medicinal plants, such as having potential value in improving the quality, yield, and stress resistance of medicinal plants. It is in short supply in medical production. This study improves its characteristics and increases its yield through genetic modification methods. In this study, the agrobacterium tumefaciens strains 15834, A4, QuarA4, MSU440, and K559 were utilized to genetically transform Physalis Calyx Seu Fructus (PCF), with the aim of further exploring the performance of the multicopy transgenic system. PCF transformed with Ri plasmid and non-transgenic PCF were employed as experimental subjects to analyze changes in their phenotypes and biological parameters. This analysis aims to support the selection of superior transgenic apomictic lines and to provide a theoretical basis for a deeper understanding of gene expression control mechanisms and gene function. Subjects and Methods: Induce the callus of medicinal plant Physalis Calyx Seu Fructus (PCF), establish an asexual reproduction line, and screen transgenic plants that stably synthesize secondary metabolites. This study adopted the method of expressing the rol key enzyme gene to cultivate transgenic plants. Different optical density (OD) values were utilized to assess the infective impact of the agrobacterium solution on pre-prepared explants, which included leaves and sterile healing tissues. An observation group and a control group were established. The bacterial solution was absorbed entirely through sterile filter paper. The contaminated explants were then placed in 1/2MS medium and incubated at 28°C in a dark environment for a duration of 2 to 3 days. Surface decontamination was performed using 1/2MS medium supplemented with 50mg/ Lantibiotics, and the decontamination process was repeated in 3-day intervals until no further growth was observed.

Results

Agrobacterium-mediated method is applied in genetic transformation research of medicinal plants such as PCF and is a relatively classical method. The optimal outcomes were achieved with an OD value of 0.5 and an infestation duration of 10 minutes on sterile healing tissues or young leaves, leading to an 60.3% induction rate of hairy roots. Conversely, employing plant material with an OD of 0.7and manipulating stem tissues for 5-8 minutes resulted in a hairy root growth rate of 70.6%. Transgenic plants were randomly selected to assess their response to agrobacterium rhizogenes strains ATCC15834, A4, QuarA4, MSU440, and K559,ATCC15834 inducing the highest efficiency of hairy roots.. Following PCR detection, a target DNA molecule of 865bp was identified.

Conclusions

The rolB gene from the Ri plasmid of agrobacterium tumefaciens has been successfully integrated into the genome of the medicinal plant PCF, confirming that the hairy roots observed in this experiment were a result of agrobacterium tumefaciens infection. After acclimatization, the survival rate of tissue culture regenerated plants transplanted to the field exceeds 80%, and their growth performance is normal. The establishment of a high-frequency and complete regeneration system lays the foundation for further genetic manipulation and the expansion of medicinal resources.

Acknowledgements

This work was supported by a project grant from Heilongjiang Programs for Education Foundation (Grant No. KYYWF-0237). BEPS24029: DEVELOPMENT AND APPLICATION OF MULTIFUNCTIONAL HEALTHY ELDERLY NURSING PADS Ying Zhoua, Wenbin Wua aChengde College of Applied Technology, Chengde, 067000, china.

Background

This paper discusses the application of multifunctional healthy elderly care mats in daily care for the elderly and its advantages. With the aggravation of the trend of population aging, there is an increasing demand for high-quality daily care for the elderly, and at the same time, the maintenance of their physical health has also put forward higher requirements. Multifunctional elderly care pads integrate multiple functions such as antimicrobial, comfort, breathability, health care, and convenient replacement by embedding traditional Chinese medicine chip technology. These features not only effectively reduce the risk of skin infections and bedsores caused by prolonged bed rest, but also improve the quality of sleep and comfort, which plays a positive role in maintaining the health of the elderly. In addition, its convenient replacement and maintenance design reduces the workload of caregivers, improves the self-care ability of the elderly, and helps to further improve the health of the elderly. Subjects and Methods: According to the design concept of the healthy elderly care mat, through the selection and optimization of materials, the integration of traditional Chinese medicine health care functions, the structural design and process optimization, and the use of advanced preparation methods, we ensure that the care products can be presented to consumers in the best state, and all-around guarantee the quality and safety of the products, so as to safeguard the health of consumers.

Results

The research, development and application of multifunctional healthy elderly care mats significantly improve the quality of life of the elderly, reduce the burden of caregivers through a variety of advanced technologies, enhance the life satisfaction and happiness of the elderly, and promote the development of the intelligent elderly care industry. These innovations not only satisfy the needs of the elderly for a high-quality and healthy aging life, but also provide more convenient and efficient care solutions for senior care institutions and families, showing broad market prospects and social value.

Conclusions

As an innovative achievement in the field of modern healthcare and elderly care, the multifunctional elderly care mat, with its multiple advantages of antibacterial and anti-odor, comfort and breathability, healthcare and physiotherapy, and convenient replacement, provides the elderly with a more comprehensive, efficient, and comfortable solution for daily care. The emergence of this product has effectively responded to the challenges of care brought about by the aging population, and has also significantly improved the quality of life and health of the elderly. Acknowledgments: 2023 Chengde City Applied Technology Research and Development and Sustainable Development Agenda Innovation Demonstration Zone Special Project “Multi-functional Elderly Nursing Care Pads (Pads) R & D and Application”. BEPS24030: A CASE OF PULMONARY INTRAVASCULAR LARGE B-CELL LYMPHOMA DOMINATED BY DIFFUSE GROUND-GLASS SHADOW IN BOTH LUNGS AND LITERATUREV REVIEW Xiuyan Wanga, Shaofeng Suna, Ping Lia, Hong Zhanga aGeneral Hospital of Ningxia Medical University, Yinchuan, Ningxia Province, China.

Objective

To investigate the clinical features, imaging features and treatment of pulmonary intravascular large B-cell lymphoma (IVLBCL) with diffuse ground glass shadows in both lungs, and to provide help for the early diagnosis of the disease. Subjects and Methods: A 54-year-old female patient was presented with chest tightness and shortness of breath for 2 months, aggravated for 4 days. No other symptoms prior to this illness. There were no symptoms of cough, phlegm, fever or chest pain during this illness. A chest computed tomography (CT) scan revealed diffuse ground-glass shadow in both lungs. The patient was initially diagnosed with pneumonitis based on the result of based on chest CT in local hospital. After treatment for 2 weeks, the patient’s shortness of breath is getting worse, with no resolution of lesions on chest CT. No obvious thrombosis was found in CT pulmonary angiography (CTPA). No significant abnormality was found in electrocardiogram, ultrasonic cardiography and polysomnography. Lung function showed early changes in small airway function, normal ventilation function, lung reserve function and lung volume, mild diffusion disorder. ultrasonography of deep veins of both lower limbs showed no abnormalities in deep veins of both lower limbs. The patient consented to positron emission tomography (PET)/CT. It showed that fluorodeoxyglucose (FDG) metabolism was significantly increased in the both lungs, skeleton, and spleen. Large B-cell lymphoma was diagnosed by bone marrow and lung puncture biopsy. Pathology examination results suggested lung infiltration of B lymphohematopoietic system tumors. The patient was finally diagnosed as intravascular large B-cell lymphoma (IVLBCL). IVLBCL with diffuse lung ground glass lesions is very rare and difficult to diagnose.

Results

The patient presented with clinical shortness of breath after activity, and the pathological characteristics of lung biopsy showed that abnormal lymphocytes were found in the capillaries and small vessels of the lung tissue, and the final diagnosis was pulmonary intravascular large B-cell lymphoma. After aggressive chemotherapy, the patient’s clinical symptoms were significantly relieved, and the imaging examination showed ground glass shadow absorption in both lungs.

Conclusions

Pulmonary IVLBCL has no specific clinical manifestations and is easily misdiagnosed as interstitial lung disease with rapid progression and poor prognosis. Therefore, early diagnosis and treatment can improve the prognosis.

Acknowledgements

This work was supported by research project of Ningxia Medical University (Item number: XJ2023002001). BEPS24031: EFFICACY OBSERVATION OF HEXUE MINGMU TABLETS COMBINED WITH METFORMIN IN TREATING DIABETIC RETINOPATHY Xiaoqing Denga, Lijuan Sunb aDepartment of Traditional Chinese Medicine Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

To observe the efficacy of Hexue Mingmu tablets combined with metformin in the treatment of diabetic retinopathy.

Methods

A total of 120 patients with diabetic retinopathy who received treatment at the Traditional Chinese Medicine Department of Handan Aiyan Ophthalmic Hospital from May 2023 to May 2024 were selected as the subjects of this study. They were divided into a study group and a control group using a random number table method, with 60 cases each. The control group was given metformin, while the study group was given Hexue Mingmu tablets on top of metformin. The treatment period was 4 weeks. Blood glucose levels, fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), postprandial 2-hour plasma glucose (2hPG), and visual acuity improvement before and after treatment were compared between the two groups.

Results

The overall effective rate of the study group was higher than that of the control group. After treatment, the levels of FPG, HbA1c, and 2hPG in both groups were lower than those before treatment, and the levels in the study group were significantly lower than those in the control group (P<0.05). The naked eye visual acuity levels in both groups improved, and the improvement in the study group was significantly greater than that in the control group (P<0.05).

Conclusion

The combination of Hexue Mingmu tablets and metformin has shown superior clinical efficacy in the treatment of Diabetic Retinopathy (DR). Hexue Mingmu tablets, an effective traditional Chinese medicine, play a role in improving retinal microcirculation by promoting blood circulation, removing blood stasis, clearing liver heat, and brightening vision. Metformin, on the other hand, is a primary medication for treating Type 2 diabetes. The combined use of these two treatments has demonstrated significant clinical effectiveness, not only stabilizing blood sugar levels but also enhancing patients’ vision and improving eye conditions. This represents an effective therapeutic approach that leverages the regulatory functions of traditional Chinese medicine in conjunction with Western pharmaceuticals for the treatment of diabetic retinopathy. BEPS24032: ESTABLISHMENT AND DEMONSTRATION OF A SURVIVAL PREDICTION MODEL FOR PATIENTS WITH ENDOMETRIAL ADENOSQUAMOUS CARCINOMA: A POPULATION-BASED STUDY Fang Dinga, Linmiao Zengb, Zhentian Wua, Muqing Wanga, Rong Chenc, Xin Liua, Kaisong Yee, Meihua Lina, Jianhua Xied, Lianghua Wua aDepartment of Hematology and Oncology, Mindong Hospital Affiliated to Fujian Medical University, Fuan, Fujian, China,bDepartment of Respiratory and Critical Care Medicine, Mindong Hospital Affiliated to Fujian Medical University, Fuan, Fujian, China,cDepartment of Otolaryngology head and neck surgery, Mindong Hospital Affiliated to Fujian Medical University, Fuan, Fujian, China,dDepartment of Radiotherapy, Mindong Hospital Affiliated to Fujian Medical University, Fuan, Fujian, China,eDepartment of Emergency medicine, Mindong Hospital Affiliated to Fujian Medical University, Fuan, Fujian, China.

Background

The goal of this investigation was to create and verify a nomogram that is capable of predicting the overall survival (OS) for individuals diagnosed with endometrial adenosquamous carcinoma (EAC). This work aimed to provide clinicians with a reliable prognostic tool to enhance the evaluation of patient outcomes, thereby supporting more informed treatment decisions and ultimately improving clinical outcomes for patients with EAC. Subjects and Methods: The medical information of 1241 eligible individuals with EAC between 2000 and 2017 was acquired from the database of Surveillance, Epidemiology, and End Results (SEER). Our objective was to evaluate survival outcomes and to detect risk factors connected with EAC. The participants were allocated randomly to training (n=868) or validation sets (n=373), ensuring that the fundamental features of the two groups were similar. Created a nomogram using the results of multivariate analysis of the training set, and then validated it using the validation set. The overall discriminant ability of nomogram was evaluated by drawing a receiver operating characteristic (ROC) curve and calculating area under the curve (AUC). Calibration curves were used to assess how well the predicted probabilities match the observed results. We applied the C-index, also known as uniform probability or Harell’s C statistic, to measure the degree to which predictions agree with observations. By plotting the relationship between the net return and the threshold probability, decision curve analyse (DCA) helped to determine the range of the threshold probability. In summary, the system’s effectiveness was evaluated utilizing ROC curve, calibration curve, concordance index (C-index), and DCA.

Results

Multivariate analysis identified age, stage, marital status, grade, radiotherapy, and surgery as predictive variables for OS in individuals with EAC. Based on these 6 independent prognostic factors, a nomogram was constructed for predicting the surviving probability at 1, 3, and 5 years. The C-indexes for the training and testing sets were 0.78 (95% CI: 0.758-0.802) and 0.763 (95% CI: 0.728-0.798), respectively. Additionally, ROC curves displayed that the model had satisfactory predictive correctness. Curves of calibration illustrated excellent consistency between the anticipated and definite values. Finally, the DCA curve highlighted the medical applicability and potential of the nomogram.

Conclusions

This study established a survival prediction model with favorable prediction accuracy and discriminatory ability to assist in tailored survival predictions for patients with EAC. BEPS24033: EFFECTIVENESS OF TRADITIONAL CHINESE EXERCISES ON WEIGHT MANAGEMENT IN OVERWEIGHT AND OBESE UNIVERSITY STUDENTS: A SYSTEMATIC REVIEW AND META-ANALYSIS Jing Liua, Yunxin Lianga, Xiang Chenb aZhejiang Chinese Medical University, Hangzhou 310000, China,bThe Second School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou 310000, China.

Background

To conduct a comprehensive assessment of the impact of traditional Chinese exercises on overweight or obese college students and to offer specific evidence to support the enhancement of their conditions through the implementation of traditional Chinese exercises. Subjects and Methods: The following databases were searched: PubMed, Embase, Cochrane Library, Web of Science, CNKI, CMJD, Wanfang, and CBM to identify randomised controlled trials that investigated the effectiveness of traditional Chinese exercise for overweight or obese college students. Until August 15, 2024, the inquiry period was established. The Cochrane5.1.0 manual was employed to evaluate the risk of bias, and Review Manager 5.4 software was employed to conduct the meta-analysis.

Results

In total, 8 literatures were incorporated, which included a sample of 353 individuals. Compared to the control group, the experimental group exhibited significant differences in the changes of body weight [MD=-6.06, 95%CI=-8.42~-3.69), p<0.00001], BMI [MD=-2.17, 95%CI=-3.01~-1.33), p<0.00001], body fat percentage [MD=-2.82, 95%CI=-3.88~-1.75), p<0.00001], waist circumference [MD=-5.38, 95%CI=-9.13~1.63), p=0.005], and hip circumference [MD=-3.68, 95%CI=-6.78~-0.59), p=0.02], as indicated by the meta-analysis results. However, there were no significant differences in the changes of waist-hip ratio [MD=-0.03, 95%CI=-0.05~-0.00), p=0.05]. Yijinjing had a more significant positive impact on the weight [MD=-13.23, 95%CI=-18.89~-7.57), p<0.00001] and BMI [MD=-3.34, 95%CI=-5.37~-1.31), p=0.001] of overweight or obese college students than Taijiquan and Baduanjin, as demonstrated by subgroup analysis. Additionally, the intervention time of ≥14 weeks had a more significant influence on the reduction of weight [MD=-6.82, 95%CI=-8.99~-4.66), p<0.00001] and BMI [MD=-2.55, 95%CI=-3.38~-1.72), p<0.00001] in those students. Less than seven days of exercise per week were more effective in reducing weight [MD=-8.05, 95%CI=-10.70~-5.40), p<0.00001] and BMI [MD=-2.79, 95%CI=-3.39~-2.18), p<0.00001] in college students who were overweight or obese.

Conclusions

Traditional Chinese workouts positively influence the weight, body mass index, body fat percentage, waist circumference, and hip circumference of overweight or obese college students. Future research necessitates additional randomised controlled trials with substantial sample sizes to investigate the effects of traditional Chinese activities on overweight or obese college students. BEPS24034: ASSESSMENT OF IMPLANT ANGLE VARIATIONS IN MAXILLARY POSTERIOR REGION BONE DEFICIENT PATIENTS Zhenyu Jia aDepartment of Stomatology, Shijiazhuang Aiyan Ophthalmology Hospital, Shijiazhuang 050000, Hebei, China.

Background

To evaluate and observe the effects of employing different implant angle placements in individuals with inadequate bone mass in the upper jaw posterior region.

Methods

A total of 106 patients requiring implant placement due to insufficient bone volume in the maxillary posterior area were selected and randomly divided into an observation group and a control group using a random number table. The observation group underwent vertical implant placement, while the control group had implants placed at a 45° angle. Comparisons were made between the two groups regarding implantation outcomes (plaque index, probing depth), success rates, peri-implant bone resorption, and patient satisfaction.

Results

The restoration outcomes for the observation group were superior to those of the control group; however, the difference was not statistically significant (P>0.05). Success Rates: The observation group achieved a 100% (53/53) implantation success rate, which was higher than the 96.23% (51/53) rate in the control group, but this difference was also not statistically significant (P>0.05). Peri-implant bone resorption was compared after a one-year follow-up between the two groups, with less resorption observed in the observation group compared to the control group, yet the difference was not statistically significant (P0.05), and both groups reported high satisfaction rates postoperatively, with no significant difference between them (P>0.05). Conclusion: This study compared the application effects of vertical and 45° angled implant placement in patients with insufficient bone volume in the posterior maxillary region. The findings indicate that there are no significant differences between the two approaches in terms of implant restoration outcomes, implant success rates, marginal bone resorption, and patient satisfaction. Although the success rate for vertical implant placement was slightly higher than that for 45° angled implants, these differences were not statistically significant. Consequently, the current conclusion suggests that the choice of implant placement angle has a minimal impact on the overall effectiveness of the implant. However, due to the limited sample size, these results should be validated in larger clinical trials. Future research could further explore the efficacy of different implant methods under various bone volume conditions, aiming to provide more treatment options and scientific evidence for clinical practice. Additionally, this study also highlights the importance of considering the specific circumstances of patients and the experience of the surgeon when selecting an appropriate implant method to enhance the success rate and patient satisfaction. BEPS24035: REVIEW OF RESEARCH ON THE TREATMENT OF HYPERURICEMIA IN HUMANS Jianping Liua,b,c, Yongtian Wub,c, Xuekai Weib aManagement Engineering College, Guangxi Polytechnic of Construction, Nanning 530007, Guangxi, China,bBama Hetai Longevity Industry Co., Ltd., Bama 547500, Guangxi, China,cWater and Health Science Research Center of Sciences Big Health Industry Research Institute of Guangxi Academy, Nanning 530007, Guangxi, China.

Background

Human beings are facing the significant challenge of high incidence and frequent occurrence of hyperuricemia. Previous research has not yet clarified the mechanism of hyperuricemia, so existing medicine cannot provide precise treatment for hyperuricemia based on symptoms. How to scientifically and efficiently treat hyperuricemia and promote human health has become a major global issue. Subjects and Methods: Using thematic and full cycle research methods, literature collection and review of previous research on the treatment of hyperuricemia were conducted from the perspective of modern mainstream medicine. Previous studies mainly focused on the formation process, mechanism of action, basic research and practical exploration of the entire treatment cycle of hyperuricemia. Based on this, in-depth analysis and research were conducted.

Results

The conclusion indicates that impaired or disordered function of the spleen or kidney organs, as well as reduced uric acid excretion in the kidneys, are the core causes of hyperuricemia. Scientific staging of hyperuricemia, precise treatment based on different stages of symptoms, and scientific selection of medication based on symptoms are the key to effectively treating hyperuricemia. Utilizing the advantages of traditional Chinese medicine and Western medicine, drug therapy, and non drug therapy for comprehensive treatment is the optimal choice for treating hyperuricemia.

Conclusions

Due to insufficient understanding of the factors influencing the onset of hyperuricemia, the complexity of treatment, and ideological preparation in previous research, especially the unclear core conclusions of the pathogenic mechanism of hyperuricemia, the selection of experimental subjects for drug development, clinical drug selection, and application lack specificity, resulting in the inability to cure hyperuricemia. It is urgent to focus on the pathogenesis of hyperuricemia, conduct in-depth research on the causal mechanisms of hyperuricemia and associated diseases, optimize the operation scenarios of hyperuricemia animal models, and innovate new ways of nutrition treatment for hyperuricemia. To address and solve the major challenges of high incidence of hyperuricemia worldwide, it has great scientific value and practical significance. BEPS24036: INFLUENCE OF JIUQIN EXERCISE ON GUT MICROBIOTA AND IMMUNE FUNCTION IN OVERWEIGHT/OBESE ELDERLY MEN Yu Liua, Mingjun Zhanga aSchool of Physical Education, Huizhou University, Huizhou 516007, Guangdong, China.

Objective

This study aims to investigate the effects of Jiuqin exercise on the gut microbiota and immune function in overweight/obese elderly men. With aging, the physical condition of the elderly declines, and obesity and gut microbiota imbalance are often closely related to weakened immune function. As a traditional form of qigong, Jiuqin exercise has been proven to have certain health benefits through aerobic exercise and body regulation. This paper explores the impact of an 8-week Jiuqin exercise regimen on the physical changes, gut microbiota, and immune function of overweight/obese elderly men.

Methods

A total of 48 overweight/obese elderly men were selected as experimental subjects and randomly divided into an experimental group and a control group. The experimental group participated in an 8-week Jiuqin exercise program, while the control group maintained their regular daily lifestyle. During the exercise period, Jiuqin was practiced three times a week, with each session lasting approximately 60 minutes. Before and after the exercise period, relevant indicators were measured for all subjects, including changes in gut microbiota (mainly measuring the DNA content of Bifidobacterium, Bacteroides, and Enterococci), physical health status (such as BMI, weight, and waist-to-hip ratio), and immune function indicators (such as levels of IgG, IgA, IgM in B lymphocytes and T lymphocyte subsets including CD3+%, CD4+%, CD8+%, and the CD4+/CD8+ ratio).

Results

The study results indicate that an 8-week Jiuqin exercise regimen significantly improved the gut microbiota and immune function in overweight/obese elderly men. Specifically: 1. Gut Microbiota: Jiuqin exercise significantly increased the DNA content of Bifidobacterium and Bacteroides, while significantly decreasing the DNA content of Enterococci (P < 0.05). 2. Physical Health: After exercise, the experimental group showed significant reductions in BMI, weight, and waist-to-hip ratio, with statistically significant differences (P < 0.05). 3. Immune Function: Jiuqin exercise significantly increased the levels of IgG, IgA, and IgM in B lymphocytes and significantly enhanced the percentages of CD3+%, CD4+%, and the CD4+/CD8+ ratio in T lymphocyte subsets, while decreasing the percentage of CD8+% (P < 0.05). 4. Correlation Analysis: Changes in gut microbiota were significantly correlated with immune indicators. Specifically, the DNA counts of Bifidobacterium and Bacteroides were positively correlated with CD3+%, CD4+%, the CD4+/CD8+ ratio, and levels of IgG, IgA, IgM, while negatively correlated with CD8+%. Conversely, the DNA count of Enterococci was negatively correlated with changes in immune indicators and positively correlated with CD8+% (P < 0.05).

Conclusions

The results of this study indicate that Jiuqin exercise promotes the proliferation of beneficial gut bacteria, improves immune function, and reduces weight and BMI, thereby positively influencing the physical health of elderly men. Jiuqin exercise not only serves as an effective form of exercise for weight management in the elderly but also enhances overall health by regulating gut microbiota and boosting the immune system. This finding provides new theoretical and practical guidance for health interventions in the elderly population, holding significant implications for health promotion. Acknowledgments: This study was supported by the Guangdong Province Philosophy and Social Science Planning Project on Niche and Rare Disciplines, “Compilation and Creation Research on Ge Hong’s Internal Alchemy Health Preservation Literature and Health Preservation Methods” (Project No. GD21LMZTY01). BEPS24037: THE IMPACT OF OMAHA BASED DIETARY MANAGEMENT ON THE NUTRITIONAL STATUS AND PROGNOSIS RECOVERY OF COLORECTAL CANCER PATIENTS Lin Fenga, Fang Lua, Kaiwei Moa, Huamei Lia aDepartment of Anorectal Diseases, the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou 550001, Guiyang, China.

Objective

Based on the control treatment results of 80 colorectal cancer patients in a certain hospital, this article explores the effect and role of implementing dietary management for colorectal cancer patients based on the Omaha system.

Method

80 patients with colorectal cancer were screened and admitted to our hospital from June 2022 to June 2024. Under the random number table method, the participants were divided into a control group and an observation group, with 40 cases in each group. Comparison control group (regular dietary guidance: recommended balanced diet, eat more fruits and vegetables, eat less red meat and high-fat foods, maintain sufficient hydration, pay attention to personal hygiene, promote intestinal health) Observation group (adding Omaha based dietary management: conducting nutritional assessments on patients to understand their nutritional needs and dietary preferences, and then based on the assessment results, developing personalized dietary plans for patients to ensure sufficient intake of key nutrients such as protein and fiber, while limiting high-fat, high sugar, and processed foods, providing cooking guidance and recipe suggestions, ensuring that patients can easily implement their dietary plans, closely monitoring their dietary execution, adjusting their dietary plans in a timely manner to adapt to changes in their physical condition and needs, conducting regular nutritional monitoring and evaluation, as well as psychological support, to ensure the effectiveness of dietary management and promote comprehensive recovery of patients) evaluates the changes in nutritional indicators, immune function before and after intervention, and the recovery time of each group’s prognosis.

Result

The levels of albumin (ALB), transferrin (Tf), and prealbumin (PAB) were balanced in the two groups before intervention (P>0.05); After the intervention, the levels of various indicators increased, and there were significant differences in various indicators between the two groups before and after the intervention (P0.05); After the intervention, all indicators increased, and there was a significant difference in the levels of all indicators between the two groups before and after the intervention (P<0.05). The prognosis recovery time between groups showed a significant difference (P<0.05), with the observation group taking a shorter time.

Conclusion

Based on the Omaha system for dietary management, it can effectively improve the nutritional status and immune function of colorectal cancer patients, promote their recovery time, help their physical recovery, and provide strong support for improving their quality of life. BEPS24038: CLINICAL OBSERVATION ON THE TREATMENT OF ISCHEMIC OPTIC NEUROPATHY WITH SELF-PRESCRIBED TRADITIONAL CHINESE MEDICINE IN COMBINATION WITH WESTERN MEDICINE Hongyu Sua, Lijuan Sunb aDepartment of Ophthalmological Internal Medicine, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

Clinical Observation on the Treatment of Ischemic Optic Neuropathy with Self-Prescribed Traditional Chinese Medicine in Combination with Western Medicine.

Methods

A total of 106 patients with ischemic optic neuropathy who visited our ophthalmology outpatient clinic or were hospitalized from January 2022 to January 2023 were selected as study subjects and analyzed. The 106 patients were divided into groups using a random number table method: a combined treatment group of 56 patients, who received self-prescribed traditional Chinese medicine formulas combined with western medicine, and a control group of 50 patients, who underwent conventional western medicine treatment. The clinical effects (efficacy, visual acuity index, central retinal artery blood flow velocity), and adverse reactions were compared between the two groups.

Results

The total effective rate for the self-prescribed traditional Chinese medicine formula combined with western medicine treatment group was 88.33%, which was higher than the 63.33% for the control group. The difference was statistically significant (χ²=10.23, P=0.02). After treatment, both groups showed higher visual acuity indices, EDV, and PSV than before treatment, and the changes in the combined treatment group were greater than those in the western medicine treatment group, with all differences being statistically significant (P < 0.05). No adverse reactions occurred in either the combined treatment group or the control group.

Conclusion

This study observed the clinical effects of combining self-administered traditional Chinese medicine (TCM) with Western medicine in treating patients with ischemic optic neuropathy. The results revealed that compared to using Western medicine alone, the combination of self-administered TCM and Western medicine significantly improved the overall effectiveness rate (88.33% vs 63.33%). Additionally, the combined treatment group showed more significant improvements in visual acuity index and central retinal artery blood flow velocity (EDV and PSV). These findings indicate that the combination of self-administered TCM and Western medicine can effectively improve microcirculation and enhance visual quality, demonstrating good clinical efficacy. Notably, neither treatment method resulted in adverse reactions, indicating good safety. Therefore, we believe that combining self-administered TCM with Western medicine is a treatment method worthy of clinical promotion and application, especially for patients who have poor responses to conventional Western medicine or are seeking alternative therapies. Future research can further explore the specific mechanisms of different TCM formulations and their interactions with Western medicine, aiming to provide more treatment options and scientific evidence for clinical practice. BEPS24039: POLYSACCHARIDE WITH ANTI-INFLAMMATORY AND ANTIBACTERIAL COMPONENTS EXTRACTION FROM CLOVE LEAVES Yang Yanga, Yan Fana, Hui Lia aSchool of Agronomy and Life Sciences, Shanxi Datong University, Datong, China.

Background

The clove (Syzygium aromaticum) is a herb widely used worldwide and is an important tropical spice crop in global trade. Polysaccharides are major components of the herb plants and their biosynthesis is partly controlled by the genes but mostly influenced by the availability of the nutrition and determined by the various environmental factors. The presence of polysaccharides is indispensable in the composition and functional factors of drugs. It has important functions such as anti-inflammatory and antibacterial. Extracted from natural sources through various methods, natural polysaccharides display valuable biological activities, including anti-tumor, antioxidant, immunomodulatory, and blood-sugar-lowering effects. Subjects and Methods: The clove leaves are known for their therapeutic properties, including warming the stomach to counteract nausea, invigorating the kidneys to support yang energy, providing aromatic deodorization, and stimulating the appetite while supporting digestion. In this paper, clove leaves from Boya Building in Shanxi Datong University were used as experimental materials to extract polysaccharides from clove leaves by using hot water extraction method. Then, ethanol concentration and extraction temperature on polysaccharide extraction was by researched to find the best extraction rate.

Results

The main results are as follows: In the experiment with ethanol concentration as a single factor, the polysaccharide extraction rate changes significantly when the concentration of ethyl alcohol is 70%-80%. Using undiluted anhydrous ethanol achieves the highest extraction rate of 11.19%, making it the most effective condition in this experiment. In the experiment with extraction temperature as a single factor, the polysaccharide extraction rate increases slowly when it is higher than 80°C, while the polysaccharide extraction rate increases rapidly when it is lower than 80°C. It can provide basic data for later drug development and treatment.

Conclusions

In this experiment, we focused on two single factors: ethanol concentration and extraction temperature. The optimal conditions were determined to be using anhydrous ethanol and heating in a water bath at 90°C, resulting in a polysaccharide extraction yield of 11.19%. However, the extract contains proteins and other impurities, indicating the need for further purification steps to accurately determine the polysaccharide content in clove leaves. Polysaccharides can interact with human biomolecules, enabling them to regulate immune responses and support cell growth and repair. With ongoing advancements in polysaccharide research, they are emerging as critical elements in drug discovery, especially in natural medicine and health supplement development. Acknowledgments: This work was supported by the Fund Program for the Scientific Activities of Selected Returned Overseas Professionals in Shanxi Province (20240028). BEPS24040: EFFECTS OF MIAO MEDICINE “LÜ DAN THERAPY” ON FACIAL NERVE FUNCTION IN PERIPHERAL FACIAL PARALYSIS OF WIND-HEAT TYPE AND NURSING CARE EXPLORATION Ping Xua, Xianqin Wanga, Qingling Waa, Hongying Shaoa, Yuanhua Wua aDepartment of Encephalopathy, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550002, Guizhou, China.

Background

Peripheral facial paralysis, as a common and frequently occurring disease, is characterized by high incidence, prolonged course, and slow recovery. It negatively impacts patients’ facial appearance and quality of life, often leading to significant mental stress and negative emotions. Modern medicine mainly employs treatments such as hormones, antivirals, and neurotrophic therapies; however, no specific therapeutic method exists currently. Clinical studies have shown that Miao medicine, based on the “toxin disturbance causes disease” theory, achieves remarkable therapeutic effects using the “dispelling wind and regulating qi with dispersing methods” in treating joint diseases and neurological disorders. Preliminary clinical observations have also confirmed that “Lü Dan Therapy,” which disperses wind, regulates qi, and promotes blood circulation, can be applied to neurological conditions such as pain, facial nerve paralysis, and stroke. This study aims to further validate its nursing effects and safety by using boiled eggs infused with Filipendula palmata in Miao Medicine’s “Lü Dan Therapy” to treat peripheral facial paralysis, providing evidence for clinical nursing practices.

Methods

To analyze the effectiveness and safety of Miao Medicine’s “Lü Dan Therapy” in nursing intervention for wind-heat type peripheral facial paralysis, exploring its impact on treatment outcomes and clinical applicability. Eligible peripheral facial paralysis patients were randomly assigned into two groups, with 30 patients in the TCM Nursing Group and 30 in the Control Group. The TCM Nursing Group received “Lü Dan Therapy” alongside standard treatment, while the Control Group received only standard treatment. After the intervention, the two groups were compared in terms of TCM syndrome scores, clinical efficacy, and facial nerve function indicators, with statistical analysis conducted.

Results

The TCM Nursing Group demonstrated significant advantages, with a marked decline in syndrome scores and notable improvements in House-Brackmann grading of facial nerve paralysis and facial nerve function. Compared with the Control Group, the differences were statistically significant (p < 0.05). The overall efficacy rate in the TCM Nursing Group reached 96.6%, showing a significant difference compared with the Control Group (χ² = 10.42, p < 0.01).

Conclusion

Miao Medicine’s “Lü Dan Therapy” effectively alleviates symptoms of peripheral facial paralysis and promotes the recovery of facial nerve function. However, due to the limited sample size and follow-up duration, further multi-center large-sample studies are required to validate these conclusions. BEPS24041: A STUDY ON THE INTERVENTION OF SATIR MODEL ON THE PSYCHOLOGICAL CRISIS OF COLLEGE STUDENTS Min Liua, Wei Yaoa, Yingcui Xianga, Qinsi Wua, Rong Wanga aHealth and Medical College, Nantong Institute of Technology, Nantong 226001, Jiangsu, China. ML and WY contributed equally to this study.

Background

With the rapid development of society, college students are increasingly facing psychological pressure and challenges. This situation has drawn growing attention to the mental health of college students. Recognizing the importance of psychological well-being, psychological crisis intervention has emerged as a crucial component of college education management in China. It serves as an effective means to maintain students’ mental health and ensure campus safety. This study aims to explore the efficacy of the Satir model in addressing the psychological crises encountered by college students. Subjects and Methods: In this research, a total of 30 college students from Nantong Institute of Technology were meticulously selected to serve as subjects. These subjects were evenly allocated into two distinct groups: an experimental group and a control group, with an equal number of 15 participants in each. The experimental group engaged in the Satir model-based group psychological intervention, receiving 90 minutes of intervention once a week over the course of 8 weeks. Conversely, the control group was not involved in any psychological counseling activities, including both group and individual sessions. To assess the efficacy of the intervention, a series of psychological assessments were conducted with both groups. These assessments included the Self-Esteem Scale, the Self-Rating Depression Scale, the Self-Rating Anxiety Scale, and the Social Fear Scale. These tests were administered before the intervention commenced and subsequently post-intervention to scrutinize the impact of the Satir model on various psychological indicators such as self-esteem, depression, anxiety, social fear, and other related symptoms among the college students.

Results

Independent sample T test was conducted between the experimental group and the control group. Before intervention, there was no significant difference in self-esteem, depression, anxiety and social fear scores of college students (p>0.05). After the intervention of Satir group psychological program in the experimental group, the self-esteem test score of the experimental group college students was (31.06±1.57), with significant difference (p<0.01); The depression test score of college students was (35.53±6.94), with significant difference (p<0.01); The score of anxiety test of college students was (38.13±6.97), with significant difference (p < 0.01); The score of social fear test of college students was (10.26±4.62), there was significant difference (p < 0.01).

Conclusions

The Satir model-based group psychological intervention program enhances the experience and participation of college students in mental health education. This approach is beneficial for elevating the self-esteem of college students, ameliorating symptoms of depression and anxiety, and addressing the prevailing issues related to social fear.

Acknowledgements

This paper was supported by a project grant from the Education Science Planning Project of Jiangsu Province (Grant No. B/2022/01/151); the Undergraduate Excellent Graduation Design (Thesis) Cultivation Program of Nantong Institute of Technology (Grant No. BS202453); a project grant from the 2023 Industry-University Collaborative Education Project of the Ministry of Education (Grant No. 231007653133509); a project grant from General Project for Philosophy and Social Science Research of Jiangsu Universities in 2024 (Grant No. 2024SJYB1271); a project grant from a special project titled 'Research on the Reform of High-quality Public Course Teaching' in Jiangsu Province for the year 2024 (Grant No. 2024GZJX098); a project grant from the Key Discipline Construction Project of Nantong Institute of Technology (Grant No. 879005); a project grant from China Association of Business Economics 2024 annual planning project (Grant No.20252096); a project grant from National innovative training program for college students (Grant No. 202412056009Z). BEPS24042: THE IMPACT OF PERCEIVED STRESS ON CYBERCHONDRIA AMONG COLLEGE STUDENTS: THE MEDIATING ROLE OF PSYCHOLOGICAL RESILIENCE AND THE MODERATING ROLE OF PSYCHOLOGICAL CAPITAL Shaohong Chena, Mei Chenb, Lingling Daic aSchool of Accounting, Guangzhou Huashang College, Guangzhou 511300, China,bMental Health Education and Counseling Center, Guangzhou Huashang College, Guangzhou 511300, China,cSchool of Teacher Education, Guangzhou Huashang College, Guangzhou 511300, China.

Objective

In the digital era, the abundance of online health information has led to a rise in cyberchondria among college students. This study seeks to explore the relationship between perceived stress and cyberchondria, while examining the mediating role of psychological resilience and the moderating effect of psychological capital among college students. Subjects and Methods: We conducted a survey across three universities in China, targeting a diverse group of college students to assess their levels of perceived stress, cyberchondria, psychological resilience, and psychological capital. A total of 1,090 valid responses were collected, providing a robust dataset for analysis. Data were analyzed using descriptive statistics and regression analysis to determine the complex relationships between these variables.

Results

The study’s findings reveal a notably strong positive correlation between perceived stress and the incidence of cyberchondria, indicating that with the elevation of perceived stress, there is a concurrent increase in the propensity for excessive health information seeking, which in turn escalates the risk of cyberchondria. The analysis demonstrated that psychological resilience serves as a pivotal mediator in this dynamic, effectively diminishing the risk of cyberchondria by lessening the stress-induced exacerbation of health anxiety behaviors. Moreover, the moderating influence of psychological capital was particularly pronounced; students with more robust psychological capital not only exhibited superior capabilities in managing health information but also employed more proactive strategies to cope with stress, thereby significantly lowering their susceptibility to cyberchondria. These results underscore the essential roles that psychological resilience and capital play in buffering against the adverse effects of perceived stress on cyberchondria, offering valuable insights for targeted mental health interventions.

Conclusions

This study underscores the importance of psychological resilience and capital in alleviating the negative effects of perceived stress on cyberchondria among college students. It is recommended that universities enhance mental health education by offering courses and workshops that focus on stress management and critical evaluation of health information. By developing these skills, students can improve their coping mechanisms, foster better mental health, and reduce the incidence of cyberchondria. Such interventions can create a more supportive academic environment that promotes mental well-being and mitigates the risks associated with excessive health information seeking online.

Acknowledgements

This work was supported by a project grant from the Guangdong Province 2023 Educational Science Planning Project (Moral Education Special Project) titled “Undergraduate Psychological Stress Assessment and Analysis Based on Data Mining” (Grant No. 2023JKDY049). This work was also supported by a project grant from the 2022 Guangzhou Huashang College In-House Mentorship Research Project titled “Research on the Psychological Stress Assessment of College Students Based on Data Mining Technology” (Grant No. 2022HSDS24). BEPS24043: ADVANCES IN TRADITIONAL CHINESE MEDICINE THERAPIES FOR CORONARY HEART DISEASE Zhou Yua, Dong Guoa aShandong University of Traditional Chinese Medicine, Jinan 250300, China.

Background

Coronary heart disease (CHD), a leading cause of morbidity and mortality worldwide, is characterized by the narrowing or blockage of coronary arteries due to atherosclerosis. The complexity of CHD has prompted an exploration beyond conventional Western medical treatments, leading to increased interest in Traditional Chinese Medicine (TCM) therapies which encompass a wide range of interventions such as herbal medicine, acupuncture, moxibustion, acupoint application, auricular therapy, foot bath therapy, and exercise therapy. TCM approaches are grounded in a holistic view of health that aims to restore balance within the body and between the body and its environment. Over the past few decades, there has been growing evidence supporting the efficacy of TCM in improving cardiovascular outcomes, alleviating symptoms, and enhancing quality of life for patients with CHD. Subjects and Methods: This review aims to summarize the current state of research on various TCM therapies for CHD treatment, provide insights into their mechanisms of action, and highlight the potential for integrating these therapies into comprehensive care models for CHD management. Literature up to 30 June 2024, published in English and Chinese databases including PubMed, Cochrane Library, VIP Database for Chinese Technical Periodicals, Wanfang Data, CNKI, and CBM was reviewed. Studies focusing on TCM laboratory investigations and clinical randomized controlled trials were analyzed to evaluate the effectiveness of different TCM therapies in treating CHD.

Results

Oral Chinese herbal medicines help improve blood lipid profiles, reduce oxidative stress, and lower inflammation, which can stabilize atherosclerotic plaques. Acupuncture regulates autonomic nervous system function, enhances myocardial perfusion, and relieves angina by stimulating the release of natural painkillers and other beneficial chemicals in the body. Moxibustion has been found to boost cardiac function and circulation when applied to specific acupoints.

Conclusions

TCM interventions have shown promise in modulating inflammatory responses, improving lipid profiles, enhancing insulin sensitivity, and promoting cardiovascular health. Personalized TCM treatments, tailored to individual patient characteristics, can further optimize these effects. We look forward to more rigorous clinical and foundational studies that will deepen our understanding of how TCM can effectively treat coronary heart disease (CHD), aiming to improve patient outcomes and quality of life. BEPS24044: ADVANCED BLACK PHOSPHORUS NANOMATERIALS FOR INCISION HEALING Hao Guoa, Wanqiang Fenga, Meng Zhanga, Feipeng Chena, Baoping Zhaoa,b aXingyi People’s Hospital, Xingyi City, Guizhou Province, China,bGuizhou Medical University, Guiyang City, Guizhou Province, China.

Background

To describe the efficacy of Black phosphorus (BP) in wound healing applications, and to summarize the application experience. Black phosphorus (BP) has emerged as a transformative agent in the biomedical sector, particularly spotlighting its potential in wound healing applications. The multifaceted nature of wounds and the intricacies associated with their healing mechanisms have necessitated the exploration of innovative therapeutic strategies. Recently, research on and the application of nanomaterials such as graphene, carbon nan-otubes, and metal–organic frameworks has become increasingly popular in tissue engineering. In2014, a two-dimensional sheet of black phosphorus (BP) was isolated from massive BP crystals. Since BP has attracted significant attention as an emerging nanomaterial. BP nanomaterials have many advantages, such as excellent optical and mechanical properties, electrical conductivity, good biocompatibility and good biodegradability, all of which make them particularly attractive in the biomedical field. Subjects and Methods: Black phosphorus can be used as a highly efficient drug carrier to carry drugs and transport them to the lesion site to achieve the purpose of targeted therapy. It can also carry out PTT, PDT, sonodynamic therapy, and combine with chemotherapy or gene therapy, showing great advantages in wound healing treatment. Moreover, not only can black phosphorus play its special role in tumor, but also gradually show its advantages in antibacterial and other disease treatment or detection. Based on this, using black scale as a carrier, synthetic materials prepared by using the properties of black scale have great potential in wound healing. Results: The advantages and synthetic methods of BP were summarized, and the application of BP in promoting incision healing was emphasized. Finally, the challenges and prospects of BP in incision healing were briefly discussed.

Conclusions

Black phosphorus (BP) has emerged as a notable player in the biomedical field, particularly in the context of incision healing. BEPS24045: A COMPETITIVE INTERACTION OF ARBIDOL WITH HUMAN SERUM PROTEINS AND ITS MOLAR BINDING RATE IN PLASMA Yongchun Liua, Fei Lib, Huili Qia, Yingying Lia, Nana Yanga, Minyue Guoa aCollege of Petroleum and Chemical Engineering, FLUOBON Pilot Base of Chemical Engineering, Longdong University, Qingyang 745000, Gansu, China,bCentral Blood Station of Qingyang City, Qingyang 745000, Gansu, China.

Background

The binding of drug to serum proteins affects its transport, diffusion and distribution in vivo. In addition, the binding rate between serum proteins and arbidol has not been reported yet. Subjects and Methods: The interaction characteristics and competitive relationship of arbidol with major human serum proteins, the molar binding rate between arbidol and proteins in plasma, and the effect of pH on the binding will be investigate in vitro. Fluorescence spectrometry, BIOVIA Discovery Studio 2019, Pymol software, centrifugal ultrafiltration and UV-vis spectrometry were used to perform experiments and calculate the related parameters.

Results

The binding of arbidol to IgG is of non-specific. The binding of HSA to arbidol is stronger than those of IVIg and Fibrinogen, and stronger than those of HSA to most drugs. The static types of quenching mechanism occur between protein fluorescent groups and arbidol. There exist typical hydrophobic interactions and entropy driven in the binding of arbidol to HSA, IVIg and Fibrinogen. If the influence of the other serum proteins and factors on drug binding is ignored, and only one binding site is considered, then 1/38.18 of mole fraction of arbidol can be bound by IgG and targeted to the lesion site, while 16.14/38.18 (42.27%) of mole fraction of arbidol can be bound by HSA and transported to cell surfaces. When the molar ratios of arbidol to proteins change from 1:1 to 6:1 in plasma, the molar binding rates change from 95.71±1.60% to 98.90±1.26% (p < 0.001), which has not a concentration-dependence relationship, and the average molar binding rate is 97.14±0.85%. The different pH inside and outside the cells has no obvious effect on the binding of IVIg to arbidol, but has a great effect on HSA or Fibrinogen binding to arbidol. The bindings of HSA or Fibrinogen to arbidol will be rapidly dissociated to a large extent under low pH conditions (pH ≤ 6.80).

Conclusions

There exist competitively interactional relationships between arbidol with main human serum proteins. The clinical disposition of arbidol is likely to be affected by differences in protein binding. In the future clinical application, a detergent with a drug combination stoichiometry ratio of 4~6:1 can be recommended for intravenous injection of IVIg (high-dose infusion), which may increase the distribution and bioavailability of the drugs at the lesion site, enhance the efficacy of the drugs, and improve the immune status of patients under immune regulation/management.

Acknowledgements

This work was supported by project grants from the Natural Science Foundation of Gansu (Grant No. 21JR7RM187), Qingyang Science and Technology Plan Project (Grant No. QY2021A-S069), Science and Technology Commissioner Special Project of Gansu province (Grant No. 22CX8GM138) and Youth Doctoral Project of Gansu province (Grant No. 2023QB-016). BEPS24046: DAPAGLIFLOZIN IN THE TREATMENT OF TYPE 2 DIABETES WITH CHRONIC HEART FAILURE: AN OBSERVATIONAL STUDY ON ITS EFFICACY Junhua Wanga, Lijuan Sunb aDepartment of Internal Medicine, Handan Aiyan Hospital, Handan 056000, Hebei, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

Patients with Type 2 Diabetes Mellitus (T2DM) are prone to cardiovascular complications due to prolonged hyperglycemia, among which chronic heart failure is one of the more severe complications. In recent years, the role of microRNA (miRNA) in cardiovascular diseases has gradually gained attention. Specifically, microRNA-423-5p has been shown to be closely related to changes in cardiac function and structure. Dapagliflozin, as a sodium-glucose cotransporter 2 inhibitor, reduces blood glucose by promoting urinary glucose excretion and has potential benefits for improving cardiovascular outcomes. Studies suggest that dapagliflozin may positively affect the treatment of chronic heart failure by regulating the expression of miR-423-5p, thereby influencing the metabolism and function of myocardial cells. Therefore, exploring the impact of dapagliflozin on plasma miR-423-5p in T2DM patients with chronic heart failure and its relationship with cardiac function improvement is crucial for understanding its cardiovascular protective mechanisms. To explore the impact of dapagliflozin on plasma microRNA-423-5p in patients with Type 2 Diabetes Mellitus (T2DM) combined with Chronic Heart Failure (CHF), and its relationship with improved cardiac function.

Methods

This study employed a randomized, controlled clinical trial design, enrolling 122 patients with T2DM and CHF who visited our hospital from January 2021 to December 2023, aged between 18-80 years, with a disease duration of 1-10 years. Patients were randomly divided into two groups: the control group (n=61) received conventional hypoglycemic treatment, while the experimental group (n=61) was treated with dapagliflozin for glucose lowering, with all other interventions being identical. The outcomes before treatment, and at 3 and 6 months after treatment were observed.

Results

After six months of treatment, the left ventricular ejection fraction (LVEF), stroke volume (SV), and left ventricular fractional shortening (LVFS) in the dapagliflozin group were higher than those in the control group, and the left ventricular end-diastolic diameter (LVEDD) was lower, with statistically significant differences (P<0.05). Both groups showed a significant reduction in LVEDD compared to pre-treatment levels, while LVEF, SV, and LVFS significantly increased post-treatment (P<0.05). After treatment, levels of NT-proBNP and minRNA-423-5p in the experimental group were lower than those in the control group and before dapagliflozin intervention (P<0.05). Correlation analysis showed that post-intervention plasma minRNA-423-5p levels were positively correlated with NT-proBNP levels and negatively correlated with LVEF levels. Additionally, improvements were observed in body weight, blood pressure, and lipid profiles in the experimental group (P<0.05). In terms of safety, the incidence rate of adverse reactions to dapagliflozin was low, and patient tolerance was good.

Conclusion

Dapagliflozin can effectively reduce blood glucose levels in T2DM patients with CHF, improve cardiac function indicators and prognosis, and has a relatively low incidence rate of adverse reactions. Therefore, it can be widely applied and promoted in clinical practice. BEPS24047: LSD1 INHIBITOR TRANYLCYPROMINE INDUCES TH1 LYMPHOCYTE POLARIZATION VIA THE T-BET/PSTAT1 PATHWAY Yanjuan Zhanga,b, Yongchao Liua, Xiaohua Yuana,b, Chunzi Xub, Wenjie Hub, Shan Daia,c, Jie Zhanga,d aCollege of Medicine, Nantong University, Nantong 226001, Jiangsu, China,bAffiliated Maternity & Child Health Care Hospital of Nantong University, Nantong 226001, Jiangsu, China,cDanyang People’s hospital, The Department of Blood Transfusion, Zhenjiang 212300, Jiangsu, China,dThe Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China. YZ and YL contributed equally to this study.

Background

Tranylcypromine (referred to as TCP hereafter, also known as Parnate), is a medication that holds a pivotal role in clinical practice as an effective antidepressant. Beyond its antidepressant properties, TCP distinguishes itself as an irreversible inhibitor of monoamine oxidase (MAO), a critical enzyme in neurotransmitter metabolism. Intriguingly, recent research has uncovered TCP’s potent ability to suppress the enzymatic activity of lysine-specific demethylase 1 (LSD1), a key regulator of gene expression. To delve deeper into TCP’s mechanisms of action, this study aimed to evaluate the direct impact of LSD1 chemical inhibitor TCP on helper T lymphocytes (Th cells) derived from healthy individuals. Subjects and Methods: Utilizing flow cytometry, the researchers observed that exposure to TCP led to a notable inhibition of proliferation in CD4 T cells, indicating a suppression of their growth and division. Furthermore, TCP treatment was found to induce apoptosis, a programmed cell death process, in these cells. Additionally, the phenotypic analysis revealed a repolarization of CD4 T cells towards a Th1 phenotype, characterized by a distinct set of functional properties and cytokine production profiles.

Results

To gain further insights, the study analyzed the levels of Th1 cytokine transcripts following TCP treatment. The results demonstrated that TCP could effectively induce the expression of interferon-gamma (IFN-γ), a key cytokine in Th1 responses. This induction was mediated through the activation of T-bet, a transcription factor that promotes Th1 differentiation, and the phosphorylation of signal transducer and activator of transcription protein 1 (pSTAT1), a downstream effector of IFN-γ signaling. Moreover, the study investigated the effects of TCP under conditions that favor Th2 differentiation, characterized by a strong interleukin-4 (IL-4) stimuli and the absence of interleukin-12 (IL-12). Despite these Th2-skewing conditions, TCP was able to force a Th1 polarization of CD4 T cells, suggesting its robust ability to override the existing differentiation cues. To further confirm the role of LSD1 in these processes, the researchers employed shRNA-mediated knockdown of LSD1 or treatment with TCP, a chemical inhibitor of LSD1. Both approaches led to the transcriptional activation of Th1 cytokines and an elevation in the dimethylation of lysine 4 on histone H3 (H3K4me2), a histone modification associated with gene activation.

Conclusions

In summary, these findings provide compelling evidence that TCP directly influences CD4+ T cells and enhances Th cell responses in a Th1-dominant pattern by inhibiting LSD1 activity. The involvement of T-bet and pSTAT1 molecules in this pathway suggests a complex regulatory mechanism through which TCP exerts its effects on T cell differentiation and function. Acknowledgments: The study was supported by grants from Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (10KJB310010), PAPD (A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions) and A Project Sponsored for Scientific Innovation Research of Jiangsu College Graduate. Dr. Zhang led the project, conceived and designed the experiments, and acquired funding; Y. Zhang conducted all experiments, designed figures, and wrote the manuscript; S. Dai provided guidance, advice, and expertise; Y. Liu and X. Yuan contributed technical assistance; C. Xu and W. Hu contributed statistical analysis assistance. We are very grateful to all blood donors. BEPS24048: APPLICATION OF INVISIBLE ORTHODONTIC TREATMENT WITHOUT BRACKETS IN FEMALE PATIENTS WITH SKELETAL CLASS I MALOCCLUSION COMBINED WITH PERIODONTITIS Dapeng Liua aDepartment of Stomatology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

To observe the application effect of invisible orthodontic treatment without brackets in female patients with skeletal class I malocclusion combined with periodontitis.

Method

108 female patients with skeletal class I malocclusion combined with periodontitis admitted to Handan Aiyan Ophthalmology Hospital were selected. According to the random number table method, patients were randomly divided into an observation group and a control group, with 54 cases in each group. The observation group received invisible correction without brackets, while the control group received self-locking bracket orthodontic appliances. Compare the probing depth (PD), gingival index (GI), gingival bleeding index (SBI), plaque index (PLI), gingival crevicular fluid inflammatory factor levels, misaligned teeth orthodontic time, tilted teeth correction time, serum prostaglandin E2 and substance P levels between two groups.

Results

Before treatment, there was no statistically significant difference in the periodontal index PD/GI/SBI/PLI levels between the two groups (P>0.05). After treatment, the periodontal index improved in both groups, but the improvement in the observation group was better than that in the control group, and the difference was statistically significant (P0.05). After treatment, the inflammatory factors in gingival crevicular fluid in both groups were higher than before treatment, and the observation group was lower than the control group, with a statistically significant difference (P<0.05). Follow up data showed that the observation group had a shorter time for correcting misaligned teeth and inclined teeth compared to the control group, and the difference was statistically significant (P0.05). After treatment, PEG2 levels decreased and SP levels increased in both groups, and the change in the study group was greater than that in the control group, with statistical significance (P<0.05).

Conclusion

The application of invisible orthodontic treatment without brackets in female patients with skeletal class I malocclusion combined with periodontitis can significantly improve the periodontitis condition of patients, correct misaligned deformities as soon as possible, and achieve good results, which is worthy of clinical promotion and application. BEPS24049: AN ANALYSIS OF CONFLICT RESOLUTION STRATEGIES IN HERO CHARACTERS OF CHINESE MYTHOLOGICAL ANIMATED FILMS BASED ON MBTI (1980-2024) Hang Zhaoa, Baolai Zhangb, Sheng Xuc aLiming Vocational University, Quanzhou, 362000, China,bSangmyung University, Cheonan, 31066, Korea,cNanning Vocational and Technical University, Nanning, 530008, China.

Objectives

Recent years have seen rapid growth in Chinese mythological-themed animated films, yet research on the personality traits of their hero characters remains limited. This study employed the MBTI theory to analyze the personality traits, psychological needs, and conflict resolution patterns of heroic characters in these films, thereby revealing their personality characteristics and behavioral motivations. This analysis further explores strategies for resolving character conflicts in animations. The findings of this research may offer valuable insights for scriptwriters and animators in creating more compelling and relatable characters.

Methods

This study analyzes heroic characters in Chinese mythological films from 1980 to 2024 to understand how different personality types approach and resolve conflicts. To this end, 22 representative Chinese mythological films were selected as research samples based on their viewership ratings on Douban Movie, a popular Chinese movie rating platform. Psychological measurement techniques were employed to extract character data on how these heroic characters face challenges and conflicts, and the MBTI analysis tool was utilized to explore their psychological types. The focus of the study is to reveal the psychological dynamics, behavioral patterns, and conflict resolution strategies of different animated character types when facing conflicts, aiming to provide a new perspective on the impact of personality differences on conflict management.

Results

Findings reveal that E-type hero characters tend to use direct, outward strategies in conflict resolution. Ne-type characters favor innovative, divergent thinking, while Se-type characters rely on experience and practical action. In contrast, I-type hero characters prefer introspective strategies. Ti-type characters emphasize logical reasoning and strategic planning, whereas Fi-type characters focus on emotional experiences and personal values. MBTI can assist individual characters in identifying and fulfilling their needs, thereby promoting character development and self-actualization.

Conclusions

This study provides a psychological perspective on character conflicts in Chinese mythological animated films and broadens the application of MBTI theory in cross-cultural contexts. The findings offer valuable insights for filmmakers, character designers, and cultural psychologists, with practical recommendations for applying personality type theory in storytelling and character development.

Acknowledgements

This work was supported (in part) by the Doctoral Research Initiation Fund (DRIF) from Liming Vocational University (LRB202406); Comprehensive Reform and Quality Enhancement of Ideological and Political Work in 2023 at Sanming University, Project Number: (XG23010YW); 2024 Sanming City Social Science Planning Project, Project Number: (24006); Industrial design and Digital intelligence manufacturing Fujian Applied Technology Engineering Center of Higher Universities provides some support for this work. BEPS24050: APPLICATION OF EARLY FUNCTIONAL TRAINING COMBINED WITH ORTHODONTIC APPLIANCES IN CHILDREN WITH MALOCCLUSION Yanyan Duana aDepartment of Stomatology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

To observe the application of early functional training combined with orthodontic appliances in children with malocclusion.

Method

100 children with malocclusion admitted to Handan Aiyan Ophthalmology Hospital were selected. The children with malocclusion included in the study were randomly divided into an observation group and a control group, with 50 cases in each group, using a random number table method. The control group was treated with maxillary expansion appliance combined with muscle functional orthodontic appliance, while the observation group received early functional training on the basis of the control group. After treatment, compare the changes in joint space and condyle, X-ray cephalometric measurements, and corrective effects between the two groups.

Result

Before treatment, there was no statistically significant difference (P>0.05) in the comparison between the two groups in terms of joint space, inter joint space, joint posterior space, ankle process height, ankle process inner and outer diameters, and ankle process anterior and posterior diameters. After correction, there was an increase in the posterior joint space and a decrease in ankle height in both groups, with the observation group showing a greater degree of change than the control group, and the difference was statistically significant (P<0.05). After orthodontic treatment, there were no changes in the joint space, inter joint space, ankle process inner and outer diameters, and ankle process anterior and posterior diameters between the two groups, and the differences were not statistically significant (P>0.05). There was no statistically significant difference (P>0.05) in the levels of SNA, SNB, ANB, FH-MP, SN-MP, and Ptm ANS between the two groups of males and females before treatment. After treatment, the levels of SNA, SNB, ANB, FH-MP, SN-MP, Ptm ANS in both male and female groups increased, and the observation group was higher than the control group, with statistical significance (P0.05). After correction, the protrusion distance and inclination angle of the upper and middle teeth in both groups were lower than before correction, and the observation group was lower than the control group, with no statistically significant difference (P<0.05). The completion time of treatment, correction time of tilted teeth, and correction time of displaced teeth in the observation group were shorter than those in the control group, and the difference was statistically significant (P<0.05).

Conclusion

The combination of maxillary arch expander and muscle functional appliance with early functional training can achieve good orthodontic effects and is worthy of clinical promotion and application. BEPS24051: STRUCTURAL DESIGN OF THE VARIABLE STIFFNESS JOINTS IN A REHABILITATION ROBOT Yongli Zhua, Xiaohui Heb, Xiaohui Xua aChongqing Vocational Institute of Engineering, Chongqing 402260, China,bChongqing Polytechnic University of Electronic Technology, Chongqing 401331, China.

Background

With the continuous development of robot technology, the application scope of joint rehabilitation robot is more and more widely. As the core component of the joint rehabilitation robot, the joint determines the overall performance of the robot. In order to improve the human-machine safety and environmental adaptability of the joint rehabilitation robot in different stages of rehabilitation training, the joint of the traditional rehabilitation robot is difficult to cope with the emergency and disturbance impact in the complex rehabilitation training environment. Subjects and Methods: First of all, the joint variable stiffness adjustment mechanism of the rehabilitation robot was designed. Based on the analysis of the existing mechanical stiffness regulation principle and the cam mechanism stiffness regulation principle, an integrated variable stiffness joint of a rehabilitation robot is designed. Then, the force analysis of the stiffness adjustment mechanism of the rehabilitation robot based on the cam mechanism was carried out, and the mathematical model of the joint output torque and stiffness of the rehabilitation robot was established. Based on the principle of joint stiffness regulation and the stiffness mathematical model, the key influence parameters of joint stiffness of the rehabilitation robot are extracted and analyzed. Considering the joint stiffness characteristics of rehabilitation robot under practical application, the joint stiffness influence parameters of rehabilitation robot are reasonably designed.

Results

Finally, the parameters are determined, and the joint stiffness model of the rehabilitation robot is verified through the simulation software maltab, and the main and passive variable stiffness characteristics of the rehabilitation robot joint are summarized.

Conclusions

The results show that the joint of the rehabilitation robot has a reasonable stiffness adjustment range, as well as the main and passive stiffness regulation ability in the process of dynamic movement, to meet the needs of the joint stiffness changes in the working process of the rehabilitation robot.

Acknowledgements

This work was supported by the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No.KJQN202203429); the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No.KJZD-K202203404); the Science and Technology Research Program of Chongqing Education Commission of China (Grant No. KJQN202403436). BEPS24052: IMPACT OF EXERCISE AT DIFFERENT POSTPRANDIAL TIMING ON BLOOD GLUCOSE IN TYPE 2 DIABETES PATIENTS Tie Lia, Jiaying Qib, Yujie Dingc, Li Wangb aSchool of Sports & Physical Education, Huizhou University, Huizhou 516007, China,bSchool of Nursing, Suzhou Medical College of Soochow University, Suzhou 215006, China,cThe Second People’s Hospital of Changshu, Changshu 215501, China.

Background

To explore the effect of different exercise timing on blood glucose regulation, and to provide scientific exercise prescription for type 2 diabetes patients. Subjects and Methods: Fifteen patients with type 2 diabetes were selected to take 2 Km fast walking exercise 1h, 2h and 3h after breakfast, which were defined as 1h group, 2h group and 3h group. The preset speed of each exercise is 4~4.5Km/h, and the average exercise time is about 25~30 minutes. All subjects were fixed in diet, medicine and daily physical activity. The three experimental days in the experiment were separated by at least three days in pairs to avoid the impact of the previous exercise. The fasting, pre exercise, 11am (before lunch) and 4pm (before dinner) blood glucose values of the patients were measured, and the changes of heart rate before and after exercise were observed.

Results

All 15 patients completed the exercise test and detection, and there was no early withdrawal due to intolerance. There was no significant difference in fasting blood glucose and pre exercise blood glucose among the three groups. The blood glucose in the 2h and 3h groups immediately after exercise was lower than that before exercise, while there was no significant decrease in the 1h group after meal. Among them, the 3h group was significantly lower than the 1h and 2h groups after meal. 11am blood glucose level was 3h group<2h group<1h group. The blood glucose level at 4pm was lower than that at 1h in 2h and 3h after meal groups, but there was no significant difference between 2h and 3h after meal exercise groups. The subjects had hypoglycemia after 2Km fast walking exercise, including 3 cases in the 3h group, 1 case in the 2h group and no case in the 1h group. Conclusion: In the middle intensity 2Km fast walking exercise at different time points, the hypoglycemic effect of 3h after meal is the most obvious, followed by 2h; There is a risk of hypoglycemia 1 to 2 hours after the 2Km fast walking exercise.

Conclusions

Among them, the probability of hypoglycemia 3 hours after the meal exercise is greater than 2 hours and 1 hour after the meal. In a word, in the exercise 1~3 hours after meal, the exercise 2 hours after meal plays an effective role in regulating blood sugar while the risk of hypoglycemia is low, which is a more appropriate exercise time for patients with type 2 diabetes. BEPS24053: INVESTIGATION OF THE MECHANISM OF AIRWAY REMODELING IN ASTHMATIC RATS THROUGH THE APPROACH OF ELIMINATING PHLEGM AND REMOVING STASIS BASED ON THE PI3K/AKT SIGNALING PATHWAY Wenli Wanga, Dongmei Lia, Yangyang Xub, Yimo Shic aDepartment of Pediatrics, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China,bLiaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China,cComputer Information Technology, Jilin University, Changchun 130012, Jilin, China.

Background

To investigate the effects of removing phlegm and expelling blood stasis on the expression of factors related to the PI3K/Akt signalling pathway in the asthma model, and to elucidate the mechanism of the removing phlegm and expelling blood stasis method on the remodeling of the airway in the rat model of asthma. Subjects and Methods: Fifty-four young SD rats were randomly divided into normal control group, model control group, model group, hormone treatment group, Min Cough Formula treatment group, and phlegm expectoration and stasis group, and the rats in each group were executed and taken on the 60th day. The general state, spontaneous activity and cough response of rats were observed. The lung tissues of rats were stained by HE staining method, and the morphology of lung and bronchial tissues was observed under the light microscope; ELISA method was used to detect the content of VEGF in serum; RT-QPCR method was used to determine the level of expression of PI3K, Akt, and VEGF mRNA in lung tissues.

Results

1. Compared with the normal control group, spontaneous activity in each group of rats was significantly reduced, and the cough response was significantly increased (P<0.05). The model group showed no significant differences, while spontaneous activity in each treatment group improved, and cough frequency decreased (P<0.05). When compared to the hormone treatment group, the group using expectorants and blood-activating agents showed slight improvements in spontaneous activity and cough response, but there was no significant statistical difference (P>0.05). 2. The results of ELISA method for detecting VEGF in serum showed that the expression of VEGF was elevated compared with the normal control group (P<0.05). The expression of VEGF in each treatment group was lower than that in the model control group (P<0.05), and the degree of improvement of VEGF in the hormone treatment group and the group of expelling phlegm and expelling stasis was different, with the hormone treatment group showing obvious improvement (P<0.05). 3.The results of PI3K, Akt and VEGF mRNA detection by PCR method showed that the expression of PI3K, Akt and VEGF in each drug administration group decreased compared with that in the model control group, but was higher compared with that in the normal control group (P<0.05), with the expression of each index in the hormone treatment group being lower than that in the group of expelling phlegm and expelling stasis.

Conclusions

1. Expelling phlegm and expelling blood stasis improves spontaneous activity and coughing in asthma model rats, improves lung inflammation, and has obvious efficacy in the treatment of asthma. 2. The method of eliminating phlegm and expelling blood stasis can regulate the expression of PI3K, Akt and VEGF through the PI3K/Akt signalling pathway, thus improving airway remodelling, which may be a target for the treatment of bronchial asthma. BEPS24054: CLINICAL EFFECT OF FEMTOSECOND LASER-ASSISTED PHACOEMULSIFICATION COMBINED WITH DIFFRACTIVE TRIFOCAL INTRAOCULAR LENS IMPLANTATION IN THE TREATMENT OF HIGH MYOPIA COMPLICATED WITH CATARACT Yan Chena, Lijuan Sunb aDepartment of Ophthalmology, Linzhang Aiyan Ophthalmology Hospital, Handan, Hebei 056000, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan, Hebei 056000, China.

Background

To study the clinical effect and visual quality of femtosecond laser-assisted phacoemulsification combined with diffractive trifocal intraocular lens (TFN100, United States) implantation in the treatment of high myopia complicated with cataract.

Methods

A retrospective analysis was performed on patients with high myopia complicated with cataract who underwent surgery in our hospital from November 2020 to November 2021, with a total of 120 eyes in 100 cases. The patients were divided into three different treatment groups: Group A: femtosecond laser-assisted combined with TFN100 implantation with a total of 31 patients with 43 eyes; Group B: 35 cases and 38 eyes in the common cataract phacoemulsification combined with TFN100 implantation group; Group C: A total of 34 cases and 39 eyes were in the femtosecond laser-assisted combined with monofocal intraocular lens (PmmingALD) implant group. After 3 months of follow-up, the recovery of visual acuity, the rate of lens loss, the incidence of night glare and halo, and the total higher-order aberration were collected from the three groups, and the defocus curves were drawn.

Results

At 3 months after surgery, the distance visual acuity of the naked eye was significantly better than that of group C in group A and group B (P0.05). Corrected distance visual acuity: there was no significant difference between the three groups (P>0.05). After correction, naked eye and near visual acuity: group A was better than group B, and group B was better than group C. The de-lens rate was significantly higher in groups A and B than in group C (P0.05). Total higher-order aberration: The patients in groups A and C were significantly lower than those in preoperative and lower than those in group B (P<0.05).

Conclusion

For patients with high myopia and cataracts, femtosecond laser-assisted simultaneous implantation of diffractive trifocal intraocular lenses can achieve good full vision and high postoperative visual quality. BEPS24055: DETERMINATION OF TERIPARATIDE CONCENTRATION IN HUMAN PLASMA BY LC-MS/MS AND EVALUATION OF ITS BIOEQUIVALENCE Danna Gaoa,b, Jing Wangc, Rui Lid, Ling Zhoud, Lihou Dongb, Huaijing Zhangb, Yunli Zhaoa aShenyang Pharmaceutical University, Shenyang 110016, Liaoning, China,bUnited-Power Pharma Tech Co., Ltd., Beijing 102206, China,cSalubris (Suzhou) Pharmaceuticals Co., Ltd., Suzhou 215400, Jiangsu, China,dUnited-Power Pharma Tech Co., Ltd., Shanghai, 201318, China.

Objective

This study aimed to develop and validate an ultrasensitive and reliable method for the determination of Teriparatide (PTH (1-34)) in human plasma and to evaluate the bioequivalence between the test preparation Teriparatide injection (PRO®) and the original reference drug FORTEO®. The method provides critical data support for clinical evaluation and practical application.

Methods

A deuterium-labeled Teriparatide was used as the internal standard for isotope dilution, ensuring accuracy and precision in quantitative analysis. Plasma samples were pre-processed using solid-phase extraction (SPE) to remove potential interferences and improve analyte recovery. Chromatographic separation was performed on an ACE Excel 3 C18-300 column (2.1 × 50 mm), which offered excellent peak resolution and reproducibility. The mobile phase consisted of ultra-pure water containing 0.1% formic acid (Phase A) and acetonitrile containing 0.1% formic acid (Phase B) with gradient elution. The flow rate was set at 0.60 mL·min⁻¹ to optimize separation efficiency. Electrospray ionization in positive mode, combined with multi-reaction monitoring (MRM), was used under mass spectrometric conditions to achieve high sensitivity and specificity. Method validation included assessments of specificity, linearity, lower limit of quantification (LLOQ), intra- and inter-batch precision, recovery, matrix effects, and sample stability.

Result

The validated method demonstrated excellent performance across all key parameters. PTH (1-34) showed a good linear relationship within the concentration range of 10.0–400 pg·mL⁻¹, with a regression equation of y = -0.0038 + 0.0038x and a correlation coefficient (R² = 0.9978), ensuring reliable quantification. The lower limit of quantification (LLOQ) was 10.0 pg·mL⁻¹, highlighting the method’s ultrasensitive detection capability. The intra-batch and inter-batch relative standard deviation (RSD) values were below 9.60%, demonstrating excellent precision and repeatability. The internal standard normalized matrix effect factor (MF) was ≤ 4.40%, indicating minimal matrix interference. Additionally, the processed samples exhibited good stability under various conditions, supporting their suitability for high-throughput analysis in clinical studies.

Conclusion

The established method provides high sensitivity, accuracy, and specificity, making it suitable for the determination of Teriparatide in human plasma. Its robustness and reliability make it ideal for high-throughput clinical analysis. This method has been successfully applied to the clinical consistency evaluation of the first domestic Teriparatide injection (PRO®), confirming its bioequivalence with the original drug FORTEO®. These results offer important data support for the clinical use and wider adoption of Teriparatide injections in practice. BEPS24056: EFFECTS OF PERIOPERATIVE TEMPERATURE ACTIVE MANAGEMENT ON PREGNANT AND POSTPARTUM WOMEN UNDERGOING CESAREAN SECTION Xiaohui Goua, Qinhu Zhangb, Sha Wanga, Zhenlaa, Yunpeng Wangb, Jiao Zhoub, Wei Hanb, Zhixin Wub, Yaqing Cuib aDepartment of Anesthesiology and Surgery, the First People’s Hospital of Xianyang, Xianyang 712000, Shaanxi, China,bDepartment of Anesthesiology and Surgery, Shaanxi Province People’s Hospital, Xi’an 710068, Shaanxi, China. XG and QZ contributed equally to this study.

Background

The objective of this study was to explore the physiological and psychological maintenance effects of active temperature management during the perioperative period for pregnant and postpartum women. Subjects and Methods: Eighty women who underwent elective cesarean section for a single, full-term fetus at a certain hospital were selected and randomly divided into an experimental group and a control group. The control group received conventional thermal insulation, while the experimental group received additional active thermal insulation measures in addition to the conventional ones. Changes in body temperature at different time points during the perioperative period were recorded and compared between the two groups, along with the surgical time, intraoperative bleeding volume, and postoperative coagulation function. The thermal comfort and overall satisfaction scores of the patients were recorded using questionnaires.

Results

There were significant differences in body temperature between the two groups at the time of entering the operating room, 20 minutes after anesthesia, leaving the operating room, and 1 hour after returning to the ward. The intraoperative bleeding volume of the experimental group was significantly lower than that of the control group, and the coagulation function of the experimental group was significantly better than that of the control group 12 hours after surgery. The thermal comfort experience and overall satisfaction with thermal insulation measures of the experimental group were higher than those of the control group, and there was a significant difference between the two groups (P 0.05).

Conclusion

Active thermal management measures can significantly protect the core body temperature of cesarean section patients during the perioperative period, and the recovery of coagulation function is beneficial to the wound healing of patients. It significantly enhances the thermal comfort experience and overall satisfaction with medical care of cesarean section patients and is conducive to their postoperative physical and mental health. The concept and measures of active temperature management are worthy of promotion and application in the perioperative period of cesarean section.

Acknowledgements

This work was supported by the Key Research and Development Plan Project of Xianyang City, Shaanxi Province (Project No. S2024-ZDYF-ZDYF-SF-3032). BEPS24057: COMBINED APPLICATION OF ULTRA-PICOSECOND LASER AND HYALURONIC ACID INJECTION CLINICAL EFFICACY OF FACIAL ANTI-AGING Suwei Liua aDepartment of Medical Cosmetology, Handan Aiyan Ophthalmology Hospital, Handan, Hebei 056000, China.

Background

To analyze the effects of a method using ultra-picosecond laser technology combined with hyaluronic acid injections in facial anti-aging treatments.

Methods

A total of 120 patients who sought facial anti-aging treatment in Handan Aiyan Eye Hospital from October 2022 to October 2023 were grouped according to the random number table method, with a total of 3 groups, including 40 cases in the hyaluronic acid group, 40 cases in the ultra-picosecond laser group, and 40 cases in the ultra-picosecond laser combined with hyaluronic acid group. The efficacy of the application, the comparison of skin physiological indicators, skin barrier function, and adverse reactions were observed.

Results

After treatment, the total effective rate of 90.00% in the ultra-picosecond laser combined with hyaluronic acid group was higher than that in the hyaluronic acid group (72.50%) and the ultra-picosecond laser group (70.00%) (P<0.05). Under the application of ultra-picosecond laser and hyaluronic acid combination therapy, the VISIA skin image analyzer detected various indicators, including transepidermal water loss (TEWL), water content of stratum corneum, and sebum content of the epidermis, showed significant improvement compared with that before treatment (P<0.05). Moreover, this combination therapy was more effective in improving these indicators than hyaluronic acid or ultra-picosecond laser alone (P0.05).

Conclusion

The combination of ultra-picosecond laser technology and hyaluronic acid injection in facial anti-aging treatment has achieved significant and positive results. This synergistic therapy not only effectively improves the physiological indicators of the skin, such as skin elasticity and hydration, but also enhances the barrier function of the skin without increasing the risk of adverse reactions. The ultra-picosecond laser stimulates collagen production, leading to firmer and more youthful skin, while hyaluronic acid injections replenish the skin’s natural supply of this moisture-retaining molecule, which diminishes with age. This dual approach addresses both the structural and moisturizing aspects of skin aging, providing a comprehensive treatment that is both safe and effective, as it does not raise the incidence of side effects, which is a common concern with more invasive procedures. BEPS24058: TO INVESTIGATE THE EFFECT OF MEIBOMIAN GLAND MASSAGE NURSING INTERVENTION IN THE TREATMENT OF DRY EYE CAUSED BY MEIBOMIAN GLAND DYSFUNCTION Lin Zhaoa aDepartment of Traditional Chinese Medical Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan, Hebei 056000, China.

Background

This study aims to analyze the efficacy of meibomian gland massage in alleviating dry eye symptoms caused by meibomian gland dysfunction.

Methods

A cohort of 100 patients, all diagnosed with meibomian gland dysfunction dry eye and treated at our hospital between July 2022 and July 2023, were evenly split into two groups, with 50 patients in each. The observation group was subjected to a specialized treatment regimen that included meibomian gland massage care, whereas the control group was administered standard, routine care. The purpose of this study was to meticulously analyze and compare the distinct effects of these two treatment modalities on the care outcomes, with the goal of identifying any significant differences in efficacy and patient response between the two groups.

Results

When compared with the control group, the observation group exhibited a notably higher total effective rate, and this difference was found to be statistically significant (P<0.05), indicating that the treatment approach used in the observation group was more efficacious. This statistical significance suggests that the intervention applied to the observation group had a meaningful impact on the treatment outcomes, which is a crucial finding in evaluating the effectiveness of the therapy. Before nursing, there was no significant difference in tear film breakup time (BUT), corneal fluorescein staining score (FLs) and meibomian gland function score (LMS) between the two groups (P>0.05).Following the nursing intervention, the corneal fluorescein staining (FLS) and tear film break-up time (BUT) scores of the observation group were considerably lower than those of the control group (P<0.05), while the eyelid margin score (LMS) was considerably higher in the observation group compared to the control group, with these differences being statistically significant (P<0.05).

Conclusion

When compared with the control group, the observation group exhibited a notably higher total effective rate, and this difference was found to be statistically significant (P<0.05), indicating that the treatment approach used in the observation group was more efficacious. This statistical significance suggests that the intervention applied to the observation group had a meaningful impact on the treatment outcomes, which is a crucial finding in evaluating the effectiveness of the therapy. BEPS24059: STUDY ON THE EFFICACY AND SAFETY OF BLOOD FLOW RESTRICTION TRAINING IN THE ELDERLY POPULATION Lei Lua, Wenfu Luoa, Erhui Lia, Zijing Lia, Fei Yanga aSports Teaching and Research Section, Basic Teaching Department, Xinjiang University of Political Science and Law, Tumushuke, Xinjiang 843900, China.

Objective

With the exacerbation of global population aging trends, improving the quality of life and health levels of the elderly has become a focal point of social concern. Blood Flow Restriction Training (BFR), as an emerging form of exercise, is gradually gaining attention regarding its application, safety, and effectiveness in the elderly population. This study aims to systematically review and analyze recent research on BFR training for the elderly, providing scientific evidence for this field and guiding future research directions. Subjects and Methods: This study reviews the research on BFR training implemented in elderly populations in recent years, covering different training intensities, frequencies, and durations. The subjects include elderly people with various health conditions. By restricting blood flow to the limbs, it observes its effects on muscle strength, muscle volume, bone density, and quality of life. In terms of safety, it focuses on analyzing potential risks such as localized ischemia, muscle damage, and deep vein thrombosis, and discusses the importance of reasonably controlling training intensity, pressure, and personalized guidance.

Results

The research results indicate that BFR training significantly enhances the muscle strength, increases muscle volume, and improves Bone density was improved, and the quality of life was enhanced. Specifically, BFR training effectively improved the muscle function and strength of the elderly by promoting muscle protein synthesis and slowing down muscle atrophy, thereby reducing the risk of falls and improving their ability to perform daily activities independently. Although there are certain health risks associated with BFR training, these risks can be significantly reduced through reasonable training design and individualized adjustments.

Conclusion

In summary, BFR training, as an innovative form of exercise, has great potential for application among the elderly population. However, individual adjustments and medical supervision should be emphasized in practical operations to ensure that the training brings positive health benefits while avoiding potential risks. Future research should continue to explore the impact of different training parameters on the health of the elderly and stress the importance of professional guidance to ensure the effectiveness and safety of BFR training in the elderly population. BEPS24060: ADRIAMYCIN LIPOSOMES REDUCE THE RECURRENCE OF CARDIAC ELECTRICAL CONDUCTION AFTER RADIOFREQUENCY ABLATION IN A RABBIT MODEL Wei Zhanga, Feng Zhangb, Junshan Wangc, Zhixuan Dingd, Ying Zhugeb aDepartment of Cardiology, Shidong Hospital Affiliated to University of Shanghai for Science and Technology, 999 Shiguang Road, Shanghai 200438, China,bDepartment of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200800, China,cDepartment of Gastroenterology, Bengbu First People’s Hospital, Bengbu 233000, Anhui, China,dDepartment of Pharmacy, Jingan District Zhabei Central Hospital, Shanghai 200070, China. WZ, FZ, JW and ZD contributed equally to this work.

Background

Radiofrequency catheter ablation (RFCA) is an advanced treatment for arrhythmias. However, incomplete ablation damage can result in the restoration of electrical conduction within the ablated region, potentially leading to the recurrence of arrhythmias, such as atrial fibrillation post-ablation. To address this issue, a novel therapeutic approach combining cardiac radiofrequency ablation with liposomal doxorubicin (L-DOX) was investigated in this study. Subjects and Methods: The scientific investigation undertaken by the researchers focused on the development of a rabbit model designed to mimic the effects of left atrial appendage (LAA) ablation injury. In this model, the researchers were particularly interested in observing the occurrence of electrical conduction recurrence, a phenomenon that can sometimes follow such ablation procedures. After the ablation was performed, the compound L-DOX was introduced into the rabbits’ bloodstream via intravenous administration. The subsequent phase of the study aimed to meticulously evaluate the drug’s targeting efficacy and its underlying mechanism of action. This evaluation was accomplished through a comprehensive pharmacokinetic analysis, an assessment of the drug’s distribution within the tissue, the utilization of fluorescence imaging techniques, and the implementation of apoptosis detection methods. Furthermore, to quantify the rate of electrical conduction recurrence, the researchers conducted S1S1 pacing at the distal end of the LAA. This pacing technique allowed for a precise measurement of the electrical conduction recurrence. Additionally, the study placed significant emphasis on examining the potential impact of L-DOX on the liver and kidney function. This thorough examination was conducted to ensure that the drug’s administration did not result in any adverse effects on these critical organs, thereby providing a comprehensive safety profile for L-DOX in the context of LAA ablation injury.

Results

One month post-radiofrequency catheter ablation (RFCA), 80% of the rabbits that received both RFCA and L-DOX exhibited sustained electrical conduction block at the ablation site. In contrast, only 20% of the rabbits in the RFCA-only group maintained such a block. The study also revealed that L-DOX specifically targeted the heart’s ablation area and enhanced the level of apoptosis among cardiac myocytes within that region. This targeted apoptotic effect of L-DOX could be the underlying mechanism that reduces the likelihood of electrical conduction recovery post-ablation. Although L-DOX was found to have short-term adverse effects on liver and kidney function, these effects were noted to be temporary and reversible.

Conclusions

This research confirmed that L-DOX can effectively target the ablation areas of the heart and decrease the incidence of electrical conduction recovery following ablation. Consequently, this novel therapeutic strategy could represent a promising new approach to enhance the efficacy and success rates of radiofrequency ablation treatments. Acknowledgments: This work was supported by grants obtained from the National Natural Science Foundation of China (82370318). BEPS24061: DIAGNOSTIC, PROGNOSTIC, AND IMMUNOLOGICAL ROLES OF FBLN1 IN PAN-CANCER: A BIOINFORMATICS ANALYSIS Rui Hana, Jianhui Lina, Wenrui Jianga, Pei Zhangb, Jincheng Xua aDepartment of Stomatology, First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, Anhui, China,bCollege of pharmacy, Bengbu Medical University, Bengbu 233000, Anhui, China.

Background

The diverse components in the tumor extracellular matrix (ECM) work in concert to promote tumor growth, invasion and metastasis. Fibulin 1(FBLN1) is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. May play a role in cell adhesion and migration along protein fibers within the ECM. Has been implicated in a role in cellular transformation and tumor invasion. Its role in human cancer initiation and progression has garnered recent attention. However, no systematic pan-cancer analysis has been conducted to explore its function in diagnosis, prognosis, and immunological prediction. Our study aims to provide a more systematic and comprehensive understanding of the role of FBLN1 in the development and progression of multiple malignancies through analysis of FBLN1 in cancers.

Methods

We explored the role of FBLN1 in cancers using The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) database. ROC curves were used to estimate the diagnostic value of FBLN1 in the 33 cancers. The “survival” package was used to conduct Kaplan–Meier (K-M) analysis. The cBioPortal was searched for genetic alteration information of FBLN1.STRING database was used to collect and integrate potential protein interactions with FBLN1.For investigating the associations between FBLN1 expression and molecular or immune subtypes in the 33 cancers, the “subtype” module of TISIDB database was performed. We explored the famous cancer-related pathways activated or inhibited in the 33 cancer types of FBLN1 on GSCALite platform.

Results

The results from our study showed that FBLN1 was negatively related to the prognosis of most cancers. Low FBLN1 expression was found in a variety of cancers and differentially expressed across molecular and immune subtypes.FBLN1 was involved in many cancer pathways. It also correlated with genetic markers of immunomodulators in various cancers.

Conclusions

Through pan-cancer analysis, we discovered and verified that FBLN1 might be useful in cancer detection for the first time. Our study elucidates the role FBLN1 plays in promotion, inhibition, and treatment of different cancers. FBLN1 is a potential cancer diagnostic and prognostic biomarker with immune, targeted, or cytotoxic therapeutic value. This work is a prerequisite to clinical validation and treatment of FBLN1 in cancers. Acknowledgments: Science and Technology project of Bengbu Medical College (2021byzd112); Natural Science Foundation Project of Anhui Provincial Department of Education (KJ2021ZD0088). BEPS24062: ANALYSIS OF THE TREND IN NEUROPSYCHOLOGICAL DEVELOPMENT AMONG 12- TO 24-MONTH-OLD INFANTS IN A DISTRICT OF XI’AN FROM 2018 TO 2023 Lin Suna, Xinhua Mab, Yazhu Wanga, Yane Yangb, Jing Liuc, Boyuan Zhangb aDepartment of Well-children, The Second Affiliated Hospital of Xi’an Medical University, Xi’an 710038, China,bDepartment of Pediatric, The Second Affiliated Hospital of Xi’an Medical University, Xi’an 710038, China,cDepartment of Outpatient Department Office, The Second Affiliated Hospital of Xi’an Medical University, Xi’an 710038, China.

Background

Early childhood development is the most critical period in a person’s life, particularly during the rapid development of the brain and nervous system from 0 to 3 years old. This study aims to reveal the time trend of influence on the neuropsychological development of children, and provide scientific basis for public health decision-making. Subjects and Methods: To analyze the neuropsychological development status and trends among 12- to 24-month-old infants in a district of Xi’an from 2018 to 2023, aiming to provide data support for early intervention strategies. A total of 5,150 infants from the outpatient department of a tertiary hospital in Xi’an were assessed using the “Neuropsychological Development Scale for Children Aged 0-6 Years”. Data on developmental quotient (DQ), gross motor skills, fine motor skills, adaptive behavior, language, and social behavior were analyzed. SPSS 25.0 software was used for data processing. For the quantitative data conforming to the normal distribution, the independent sample t test or paired t test was used for inter-group comparison. If it does not conform to the normal distribution, the rank sum test is used. Qualitative data were expressed as [n(%)], and inter-group comparisons were conducted by chi-square (χ2) test, with P < 0.05 as the standard of statistical significance.

Results

There were significant differences in developmental quotient between years. The detection rate of low DQ among 12- to 24-month-old infants increased from 1.2% to 4.3%, the suspicious detection rate increased from 20.3% to 36.6%, and the abnormal detection rate increased from 21.6% to 40.85%. Declines were observed across all five developmental domains, with fine motor skills and language being the most significantly affected.

Conclusions

Neuropsychological development among infants has shown a declining trend over time, potentially related to major environmental changes such as the COVID-19 pandemic. Children aged 12 to 24 months were affected by the epidemic in different functional areas with different sizes and durations, which may be related to the non-uniformity and complexity of brain development, and the development maturity of the nervous system will directly affect the development of children’s fine motor, gross motor, language, adaptive ability and social function areas. This study not only reveals the impact of the epidemic on the neuropsychological development of young children, but also emphasizes the importance of early intervention. Future studies should further explore the long-term impact of the epidemic on children of different ages and develop effective intervention strategies to support children’s overall development. It is recommended to strengthen parental education and promote high-quality companionship to facilitate children’s neuropsychological development, particularly emphasizing early intervention for boys.

Acknowledgements

This work was supported by a project grant from The Second Affiliated Hospital of Xi’an Medical University. BEPS24063: ENHANCING THE ANTI-FIBROTIC EFFECTS OF ISOLIQUIRITIGENIN DERIVATIVES BY TARGETING EPITHELIAL-MESENCHYMAL TRANSITION IN A549 CELLS Peiling Zhaoa, Long Zhangb aInnovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China,bBaoding Cigarette Factory, Hebei Baisha Tobacco Co., Ltd., Baoding 071000, Hebei, China. PZ and LZ contributed equally to this work.

Objective

Isoliquiritigenin (ISL), a natural flavonoid derived from Glycyrrhiza glabra, has garnered attention for its broad range of pharmacological effects, positioning it as a promising candidate for the prevention and treatment of various diseases. Pulmonary fibrosis (PF) is a progressive disorder marked by lung tissue scarring, where epithelial cells transform into fibroblasts through epithelial-mesenchymal transition (EMT). Previous studies have demonstrated that ISL can inhibit EMT; however, its clinical application is limited by its poor solubility, low bioavailability, and rapid degradation. To address these challenges, structural modifications of ISL were conducted to enhance its therapeutic potential.

Methods

A total of 17 ISL derivatives were synthesized through the Claisen-Schmidt condensation reaction. The molecular structures of these derivatives were verified using NMR spectroscopy (1H-NMR and 13C-NMR). To evaluate their anti-EMT potential, A549 cells were treated with TGF-β1 to induce EMT. The ability of these derivatives to inhibit EMT was assessed through a series of experiments, including quantitative reverse-transcription PCR (qRT-PCR) to measure gene expression, cell migration assays to observe changes in motility, and morphological staining to examine alterations in cell shape. ISL was used as a baseline control for comparison across all assays.

Results

The results revealed that both ISL and its derivatives (A1, A6, A7, A11, and A16) effectively promoted the expression of the epithelial marker E-Cadherin and suppressed the expression of the mesenchymal marker Vimentin, thereby inhibiting EMT in TGF-β1-induced A549 cells. Notably, the derivatives exhibited much stronger effects compared to ISL. In scratch assays and morphological studies, these derivatives significantly reduced cell migration and maintained the epithelial-like morphology of A549 cells.

Conclusion

In conclusion, the ISL derivatives A1, A6, A7, A11, and A16 exhibited a markedly stronger capacity to inhibit EMT compared to ISL itself. These compounds not only suppressed key markers of mesenchymal transition but also maintained the epithelial integrity of A549 cells, pointing to their potential as promising candidates for the treatment of pulmonary fibrosis. The enhanced efficacy of these derivatives underscores their therapeutic value, warranting further investigation into their anti-fibrotic effects and possible clinical implementation. BEPS24064: THE IMPACT OF MODIFIED UVULOPALATOPHARYNGOPLASTY COMBINED WITH LOW-TEMPERATURE PLASMA RADIOFREQUENCY ABLATION OF THE TONGUE BASE ON PULMONARY FUNCTION, BLOOD GAS PARAMETERS, AND QUALITY OF LIFE IN FEMALE PATIENTS WITH OBSTRUCTIVE SLEEP APNEA-HYPOPNEA SYNDROME Xiaomiao Lia, Lijuan Sunb aDepartment of Otolaryngology, Handan Aiyan Ophthalmology Hospital, Handan, Hebei 056000, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan, Hebei 056000, China.

Objective

To observe the impact of H-UPPP combined with low-temperature plasma radiofrequency ablation of the tongue base on pulmonary function, blood gas parameters, and quality of life in female patients with obstructive sleep apnea-hypopnea syndrome (OSAHS).

Methods

A total of 100 female OSAHS patients treated at Handan Aier Eye Hospital from May 2022 to December 2023 were selected and randomly divided into an observation group and a control group using a random number table method, with 50 patients in each group. The control group received traditional H-UPPP treatment. The observation group received additional low-temperature plasma radiofrequency ablation of the tongue base based on the treatment provided to the control group. After treatment, lung function, blood gas analysis, quality of life improvement, overall clinical efficacy, and adverse reactions were compared between the two groups.

Results

There was no significant difference in preoperative lung function indicators such as forced vital capacity and forced expiratory volume in one second between the two groups (P>0.05). After treatment, both groups showed improvements in lung function indicators, but the observation group had significantly better results (P0.05). Postoperatively, PaO2 increased in both groups, with the observation group showing a higher increase than the control group (P0.05). Before surgery, there was no significant difference in QSQ quality of life scores across the five dimensions between the two groups (P>0.05). Postoperatively, scores in all five quality of life dimensions improved in both groups, with the observation group showing greater improvement than the control group (P<0.05). The overall clinical effectiveness rate in the observation group was 80.0% (40/50), significantly higher than the 25% (25/50) in the control group (P0.05).

Conclusion

H-UPPP combined with low-temperature plasma radiofrequency ablation of the tongue base can significantly improve pulmonary function and blood gas parameters, enhance quality of life, and show good clinical efficacy in treating female OSAHS patients without increasing the incidence of complications. This approach is worthy of clinical promotion and application. BEPS24065: MISDIAGNOSIS OF ADNEXAL TUMOR TORSION IN A GIRL Yuan Wena, Li Zhua, Hui Tana, Jinzhi Maa, Yanfei Zhoua aYellow River Sanmenxia Hospital, Sanmenxia 472000, Henan, China.

Objective

To investigate the etiology, diagnosis and treatment of tubal abscess in girls, analyze the causes of misdiagnosis and prevent misdiagnosis.

Methods

The clinical data of a girl with tubal abscess admitted to Yellow River Sanmenxia Hospital on November 28, 2024 were analyzed. The 12-year-old girl had no sexual life history, abdominal pain with nausea, and no fever. The rectal ultrasound in the outpatient clinic suggested that the cystic mass was in the accessory area, and the left ovary was not ruled out. Considering the adnexal tumor torsion, and admission with perfect MRI, it indicated a solid mixed mass in the pelvic cavity and long T1 and T2 abnormal signal in the cystic cavity. Limited diffusion in the capsule, and significantly high signal in DWI. Considering the tubal pyyosis, high blood image and no change in mass size after antibiotic treatment, the pathological results of laparoscopic exploration surgery made a clear diagnosis: tubal pyyosis with hemorrhage and necrosis. Analysis the causes of misdiagnosis and summarize the causes of diagnosis and treatment to prevent misdiagnosis.

Results

Pelvic MRI examination was performed to exclude tumor torsion, and the size of the ultrasound mass was unchanged after antimicrobial treatment. After laparoscopic exploration was performed, he left fallopian tube was stiff, the adhesion of the ovary and the intestine was serious, and the umbrella end atresia was severe. Distal fallopian tube obstruction score: 26 points, the fallopian tube function may be seriously impaired. If retained, the successful probability of natural fertility is low and the tubal pregnancy is easy to occur, and the postoperative tubal obstruction and pus accumulation may be large after communication with the family of the patient, the girl is recovered and discharged from the hospital.

Conclusion

The girl has abdominal pain, although there is no typical fever, even in the period to be alert to the occurrence of tubal abscess, especially the girl after the menstruation is also one of the causes of pus. Rectal ultrasound combined with pelvic CT or MRI can make a clear diagnosis, avoiding misdiagnosis and blind surgery to increase the accuracy of diagnosis. BEPS24066: SUMMARY OF THE BEST EVIDENCE FOR EARLY PULMONARY REHABILITATION PROTOCOLS IN PATIENTS WITH AECOPD Wei Duana, Jiahui Yonga, Hanping Youa, Chan Zhanga aDepartment of Medical, People’s Hospital of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan 750001, Ningxia, China.

Background

Pulmonary rehabilitation is a comprehensive intervention measure aimed at improving the physiological and psychological status of COPD patients and prompting patients to adhere to health-promoting activities for a long time. Early pulmonary rehabilitation refers to the early pulmonary rehabilitation intervention after the acute symptoms of patients are controlled in hospital, aiming at improving the respiratory muscle strength and endurance of patients and improving clinical symptoms. Studies have shown that early pulmonary rehabilitation can help improve patients’ health and enhance their ability to resist diseases, thus reducing patients’ mortality. Proper pulmonary rehabilitation training can effectively improve patients’ lung function, reduce the risk of pulmonary complications, reduce symptoms such as dyspnea, and thus improve patients’ quality of life. In addition, it can also promote the self-management of patients in the process of recovery, improve their self-care ability, and thus reduce the stress of family care. Therefore, early pulmonary rehabilitation programs are of great significance for patients with AECOPD. The current research aimed to synthesize the relevant literature on the benefits of early pulmonary rehabilitation for people with acute exacerbation of chronic obstructive pulmonary disease (AECOPD), and to determine, where, how, and when exercise training are best for AECOPD patients. Subjects and Methods: According to the “6S” evidence resource pyramid model, the computer system searched CNKI, Wanfang Database, Chongqing VIP, Embase, MEDLINE, CINAHL Nursing, UPTODATE, JBI evidence-based health care database, Co-chrane Library, PubMed and other websites to search literature in the past ten years. Chinese search strategy “Chronic Obstructive Pulmonary Disease acute exacerbation/ COPD acute exacerbation/ Chronic Obstructive Pulmonary Disease/acute exacerbation” AND “early pulmonary rehabilitation/ early/ pulmonary rehabilitation/ early rehabilitation/ exercise therapy/ pulmonary rehabilitation exercise/ respiratory training/ functional exercise/ exercise prescription”, English search strategy “AECOPD/ AE/ Pulmonary Disease/ Chronic Obstructive/ Chronic Obstructive [diagnosis, physiopathology, * rehabilitation]” AND “early PR/ Lung Diseases/ Pulmonary Rehabilitation/ Obstructive/Pulmonary Disease/ Breathing Exercises [* methods]” adjusted its search strategy for different databases. The search period is from February 20, 2013 to February 20, 2023 for relevant Chinese and English literature reports in 10 years.

Results

A total of 897 articles were included. After NoteEpress screened and read the topic abstract, 65 articles were carefully read. Among them, 35 articles were evaluated for relevant literature quality. Finally, a total of 14 articles were included, including 2 articles on clinical decision, one of which was an expert consensus guideline, which was evaluated by JBI criterion. The six items of the consensus guideline were all evaluated as “yes” and had good quality. The other guideline was independently evaluated by two researchers using AGREE Ⅱ; 1 article on treatise, 1 article on RCT, 4 articles on Meta analysis, 2 articles on guidelines, 2 articles on expert consensus, 1 article on systematic review, and 1 article on evidence summary. These evidences are of high quality.

Conclusions

The best evidence of early pulmonary rehabilitation program for patients with AECOPD summarized in this study should be comprehensively judged according to individual circumstances when using the evidence, and the best program should be implemented. Acknowledgments: This research was supported by a project grant from Ningxia Hui Autonomous Region Health System Scientific Research Project (Grant No.2022-NWKY-006). BEPS24067: SELECTED HIGH-AFFINITY NANOBODIES TARGETING HUMAN-IGG BY PHAGE DISPLAY LIBRARY Dulguun Juramta,b,c, Siyi Zoua,b,c, Baiyong Shena,b,c aDepartment of General Surgery, Pancreatic Disease Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China,bResearch Institute of Pancreatic Diseases, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China,cState Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Background

Secondary antibodies are screened and stained using animal-derived anti-IgG, which might pose biological safety concerns. Recognized as the smallest antigen-binding domains from camels, nanobodies (Nbs) exhibit high affinity, stability, and low immunogenicity. They can also be engineered and expressed in both eukaryotic and prokaryotic organisms. By employing nanobodies to identify cells that produce high levels of monoclonal antibodies, researchers can bypass worries related to cost, operational complexity, and biological safety. Nanobodies, or VHHs, are easily produced antibodies used in biopharmaceuticals for cell labeling and selection. This study constructed a phage display nanobody library, identifying novel anti-Fc VHHs with high affinity for human Fc, highlighting their potential in diagnostics and therapeutics.

Methods

A nanobody phage library was developed following the immunization of a Bactrian Camel with human IgG protein on six separate occasions. Through a process involving three iterative cycles of binding, washing, panning, and amplification, utilizing human IgG protein-coated surfaces in conjunction with the phage library, two specific nanobodies were successfully identified. These nanobodies underwent selection and screening, followed by detailed analysis using prokaryotic expression systems and purification via Ni+-NTA affinity chromatography. The nanobodies exhibited robust binding properties, as confirmed by indirect ELISA and supplementary analytical techniques. The identified anti-human IgG nanobodies demonstrated high affinity and specificity.

Results

Immunizing Bactrian camels resulted in a highly diverse human-IgG nanobody phage library with a capacity of 10¹² cfu/mL and a 100% insertion rate. Using phage display technology, two nanobodies with excellent binding properties were identified. These nanobodies were efficiently expressed and showed strong, specific binding to cell surface antigens.

Conclusion

In this research, an anti-human-IgG nanobody phage library was effectively developed. Through three iterative rounds of selection via biopanning on immobilized human-IgG, the target protein was isolated, expressed, and subsequently purified utilizing nickel affinity chromatography. Comprehensive characterization of the nanobody was conducted to assess its binding specificity and affinity. The investigation culminated in the identification of a nanobody exhibiting high affinity, high specificity, and stability, with promising applications in the creation of multivalently specific antibodies and molecular detection techniques. BEPS24068: INNOVATIVE ALEX-NET MODEL BASED CYST OF JAW DETECTION IN CBCT IMAGES Guangyan Wanga, Yanan Jiaa, Aihemaiti Gulibstana, Kexin Wanga, Feng Qiaob, Duyan Gengc aSchool of Information Engineering, Tianjin University of Commerce, Tianjin 300134, China,bHospital of Stomatological, Tianjin Medical University, Tianjin 30070, China,cSchool of Electrical Engineering, Hebei University of Technology, Tianjin 300131, China.

Background

In clinical medicine, jawbone cyst represents a prevalent and often misunderstood dental disease. Its symptoms, which can include pain, swelling, and tooth mobility, resemble those of other dental ailments, thereby posing significant challenges to accurate diagnosis. The early and precise identification of jawbone cysts is crucial for effective treatment planning and the prevention of potential complications. Subjects and Methods: To address this diagnostic challenge, this study introduces an innovative cyst detection system leveraging the Alex Net deep learning model. The system utilizes Cone Beam Computed Tomography (CBCT) datasets to detect cysts on dental radiographs. By analyzing these images, the system aims to swiftly identify potential cystic lesions and their precise locations, thereby assisting doctors in making more informed diagnostic decisions. To improve the performance of the Alex Net model, the study incorporates extensive image enhancement techniques, including noise reduction, contrast enhancement, and data augmentation.

Results

The results of this study demonstrate the effectiveness of the improved Alex Net model in detecting jawbone cysts. On existing datasets, the model achieved an average accuracy of 91.87% and a peak accuracy of 99.9%. These findings underscore the model’s high cyst recognition rate and its potential to revolutionize the diagnostic process for jawbone cysts. In comparative analyses, the study revealed that other deep learning models, such as Res Net and VGG Net, exhibited inferior performance in classifying jaw cysts. This may be due to their inability to effectively extract crucial features from medical images, resulting in lower classification accuracy.

Conclusions

The findings of this study highlight the importance of choosing an appropriate deep learning model for diagnosing specific dental diseases, such as jaw cysts. The high accuracy and reliability of the improved Alex Net model suggest its potential as a valuable tool for dental practitioners. Future research should explore the potential of combining multiple deep learning models to further enhance the accuracy of diagnosing dental diseases. Additionally, refining data preprocessing and augmentation techniques can bolster the model’s generalization capabilities, making it more robust and adaptable to different datasets. By integrating deep learning with clinical medicine, more effective auxiliary diagnostic systems can be developed, ultimately improving the accuracy and efficiency of dental disease diagnosis and treatment planning. Acknowledgments: This research was supported by National Natural Science Foundation of China (52277230). BEPS24069: TO EXPLORE THE ANTI-INFLUENZA VIRUS EFFECT OF QINGGAN GRANULE BY NETWORK PHARMACOLOGY Hongyan Shena, Xuefeng Wangb aLiaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China,bDepartment of Pediatrics, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China.

Background

To explore the possible mechanism of the treatment of Qinggan granule by using the method of network pharmacology. Investigate the efficacy of positive expiratory pressure (PEP) equipment, Acapella, in the rapid recovery of lung cancer patients after thoracoscopic surgery, and to summarize the application experience. Subjects and Methods: First, predict the targets of the active ingredients of Qinggan granule according to the Swiss Target Prediction data platform. Then the cytoscape3.10.0 software was used to build the compound - target network. Through the genecard database to find the related genes of influenza. The venny graph was used to obtain the intersection targets of the targets Qinggan granule and the influenza disease targets activity. And to build the drug - the active ingredient - the key target - disease network. Using the string database to analyze the interaction of the common targets, using the cytoscape3.10.0 to visualize, Build up the protein interaction network (PPI)); Then, use the bioinformatics platform to analyze the common targets, including the gene ontology (GO) and genome database (KEGG) pathway enrichment analysis.

Results

Through the TCMSP database, a total of 155 active ingredients of Qinggan granule were screened out, and 208 corresponding targets were identified. A total of 1178 influenza-related genes were collected. 85 common genes were obtained, b my mapping and taking the intersection between Potential targets of active ingredients and influenza-related targets. Cytolscape3.10.0 was used to construct the drug active ingredient-disease target network. There are 320 nodes and 1811 sides. PPI network was established and the key targets were obtained, including TNF, IL6, TP53, JUN, AKT1, STAT3, IL1B, CXCL8, BCL2, etc. GO analysis and KEGG pathway analysis were performed, and the results showed that: The main signaling pathways enriched in intersection targets were: AGE-RAGE signaling pathway in diabetic complications, Pathways in cancer, Lipid and atherosclerosis, Fluid shear stress and atherosclerosis, Toxoplasmosis, IL-17 signaling pathway, TNF signaling pathway, etc.

Conclusions

The active components of traditional Chinese medicine can directly target the functional proteins of influenza to achieve antiviral effect. On the other hand, through its multiple components and targets, it regulates a variety of inflammatory signaling pathways in the body, improves the body’s immunity and thus improves symptoms. Qinggan granule may be responsible for the treatment of influenza by inhibiting inflammatory response and multiple related factors and pathways to resist the flu and reduce the inflammatory response caused by the flu. The main targets are TNF, IL6, TP53, JUN, AKT1, STAT3, IL1B, CXCL8, BCL2, etc. The pathway may be IL-17 signaling pathway or TNF signaling pathway, which needs further experimental verification.

Acknowledgement

Capacity Building of evidence-based Traditional Chinese Medicine for Children with Influenza (2019XZZX-LG006). BEPS24070: ACUTE PHYSIOLOGICAL RESPONSES, LONG-TERM INTERVENTION EFFECTS AND MECHANISMS OF SLOW RESISTANCE TRAINING Haitong Yua, Chengbo Yanga aDepartment of Athletic Training, Chengdu Sport University, Chengdu, Sichuan 641418, China

Background

Slow Resistance Training (SRT) is a method that involves consciously controlling and slowing down the speed of movements while performing resistance training to increase the time under tension in the muscles and induce a more intense acute physiological response. The primary objectives of this study were to examine the effects of SRT in acute physiological responses and long-term training adaptations, to elucidate the mechanisms of SRT, and to formulate recommendations for enhancing the efficiency and safety of SRT. Subjects and Methods: We searched Web of Science, SportDiscus and PubMed databases with the search terms “strength training” OR “hypertrophy” OR “power training “OR “resistance training “OR “resistance exercise “OR “weight training” OR “tempo” OR “speed” OR “cadence “OR “velocity “OR “time under tension “OR” explosive “OR “slow”. Appropriate inclusion and exclusion criteria were applied, and the selected literature was categorized into acute interventions and long-term interventions. The effects of SRT were used to explore the possible mechanisms in conjunction with physiologic findings.

Results

SRT had an important effect on training intensity and volume, which in turn altered the level of fatigue and adaptations after training and that the effects was not influenced by training experience. SRT with moderate loads and 3-6 s eccentric durations elicited more intense metabolic stress and hormonal adaptive responses than traditional training in a one-session exercise intervention; however, slower movement speeds were detrimental to strength test performance. In the long-term intervention, SRT (eccentric for 3-4 seconds, concentric for 1-3 seconds, and total single-repetition for 4-7 seconds) at low to moderate intensities (40%~70% 1RM) twice a week for 6 weeks had better maximal strength and hypertrophy effects than traditional training. Possible mechanisms of SRT include causing more muscle damage and accumulation of metabolites, boosting more secretion of testosterone and inducing more release of growth hormone.

Conclusions

SRT has demonstrated advantages in both acute and long-term interventions and has been shown to exert a positive influence on enhancing resistance training outcomes, augmenting training diversity, and mitigating injuries. SRT may be a more effective, safer option for those who need to limit the intensity of their training. BEPS24071: COMPARISON OF THE EFFICACY OF PERCUTANEOUS MECHANICAL THROMBECTOMY AND CATHETER-DIRECTED THROMBOLYSIS IN THE TREATMENT OF ACUTE MIXED LOWER EXTREMITY DEEP THROMBOSIS Jifeng Suna,c, Yidan Hub, Jun Lic, Yuewei Wanga aThe Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, China,bPeking University People’s Hospital,Qingdao; Women and Children’s Hospital, QINGDAO UNIVERSITY, Qingdao, Shandong, 266000, China,cQingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, Shandong, 266000, China.

Background

A clinical efficacy evaluation of two different interventional surgical methods for the treatment of acute mixed lower extremity deep vein thrombosis (LEDVT). Subjects and Methods: This study randomly divided patients with acute mixed lower extremity deep vein thrombosis admitted to our hospital in recent years into two groups: the percutaneous mechanical thrombectomy group (PMT) and the catheter-directed thrombolysis group (CDT). In the percutaneous mechanical thrombus aspiration group, the Poco 8F AngioJet Ultra thrombus removal catheter was used, and in the cannula thrombolysis group, the Meridian 4F single-use infusion catheter was used, and the differences between the two groups were compared in terms of the length of hospitalization, the amount of thrombolytic drugs used, the incidence of PTS, the circumference reduction rate of the affected limb, and the incidence of complications.

Results

After the end of treatment, there was no statistically significant difference (P>0.05) between the two groups of patients in terms of the perimeter difference and the incidence of bleeding on the healthy side, which means that there was no significant difference between the two groups of patients in these two indicators, which may indicate that the treatment methods adopted have similar effects or safety in these aspects. After treatment, the difference in the circumference of the thigh and the circumference of the calf on the affected side of the two groups of patients were significantly reduced compared with before treatment, and the differences were statistically significant (P<0.05), which indicates that the difference after treatment was statistically significant in terms of the circumference of the thigh and calf. The PMT group had significantly less urokinase dosage and hospitalization time than the CDT group, and these differences were statistically significant (P0.05).

Conclusions

Both percutaneous mechanical thrombectomy and catheter thrombolysis have good clinical effects, but percutaneous mechanical thrombectomy has significant clinical advantages in the treatment of acute mixed type deep vein thrombosis in the lower limbs, especially in improving thrombus clearance rate, reducing complication rate, and improving patient quality of life. It can quickly alleviate patients’ clinical symptoms, restore lower limb venous blood flow, greatly reduce patients’ pain, significantly shorten hospitalization time, and more effectively restore venous patency and protect venous valve function compared to catheter thrombolysis treatment. It is worth widely applying in clinical practice. For some patients, combined balloon dilation can achieve better thrombus clearance rates. BEPS24072: MIR-891A-5P OVEREXPRESSION PROMOTES HEPATOCELLULAR CARCINOMA PROGRESSION BY REGULATING ADRM1 EXPRESSION: IMPLICATIONS FOR THERAPEUTIC TARGETS Chen Zhanga,#, Zhao Yuana,#, Jian Wanga,#, Yuan Wanga, Lin Shia, Ziqi Shaoa, Caoyi Lia, Qiang Lia aDepartment of General Surgery, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, China. #CZ, ZY and JW contributed equally to this research.

Background

Hepatocellular carcinoma (HCC), a form of liver cancer, represents a significant threat to global health, typically presenting at late stages with few therapeutic options available. The involvement of microRNAs (miRNAs) in cancer, including HCC, is well-documented, yet the specific function of miR-891a-5p in this context has not been thoroughly investigated. The aims of this research was to examine the presence of miR-891a-5p in HCC tissues, its effects on cellular activities of HCC, and its regulatory interaction with Adhesion Regulating Molecule-1 (ADRM1), which is considered a promising therapeutic target for HCC.

Methods

Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was utilized to measure the expression levels of miR-891a-5p and ADRM1 mRNA in both HCC and surrounding non-cancerous tissues. Vectors for the overexpression and suppression of miR-891a-5p and ADRM1 were developed to evaluate their influence on HCC cell growth, movement, and invasiveness using the CCK-8, scratch, and Transwell assays. Computational analysis and luciferase assays were employed to ascertain whether miR-891a-5p directly targets ADRM1 and modulates its expression. The effects of miR-891a-5p on ADRM1 at the protein and mRNA levels were also assessed using Western blotting and qRT-PCR.

Results

It was observed that in HCC tissues, both miR-891a-5p and ADRM1 mRNA were markedly elevated. Subsequent studies indicated that these two factors enhanced the growth, mobility, and invasiveness of HCC cells. The luciferase reporter gene assays verified a direct regulatory link between miR-891a-5p and ADRM1. An increase in ADRM1 protein and mRNA levels was observed with miR-891a-5p overexpression, whereas its inhibition led to a decrease in ADRM1 expression.

Conclusion

The study concludes that miR-891a-5p, which is abundant in HCC, fosters the expansion, mobility, and invasiveness of HCC cells by modulating ADRM1 protein levels, thus impacting HCC progression. This research enhances our comprehension of HCC’s pathogenesis and offers novel targets and approaches for HCC management.

Acknowledgements

This work was supported by Excellent Talent Project of Xuzhou Medical University (XYFY2021019). BEPS24073: EVALUATION OF THE FIRST APPLICATION OF ANESTHESIA SMART-AI MODEL IN STANDARDIZED TRAINING ON ACID-BASE BALANCE KNOWLEDGE Shaonan Wanga,#, Wu Zhenb,#, Lixin Yina, Guiping Yub, Jun Gengb, Peng Guob, Shancheng Wangb aTaizhou People’s Hospital Affiliated to Nanjing Medical University, Taizhou, China,bDepartment of Anesthesiology, Jiangyin People’s Hospital, Jiangyin, Jiangsu, China #Shaonan Wang and Wu Zhen contributed equally to this research

Objective

This study aimed to evaluate the effectiveness of the Anesthesia Smart-AI model in standardized training on acid-base balance knowledge among medical residents. The primary goal was to compare AI-assisted teaching with traditional lecture-based teaching in terms of knowledge improvement, learning consistency, and trainee engagement.

Methods

A randomized controlled trial was conducted involving 95 residents from internal medicine, surgery, emergency medicine, and anesthesiology departments. Participants were randomly assigned to either the AI group (n = 45) or the traditional teaching group (TR group, n = 50). Both groups participated in a 30-minute training session on acid-base balance concepts. The AI group utilized educational content and case analysis tools generated by the Anesthesia Smart-AI model, while the TR group received lectures prepared and delivered by senior physicians. To assess knowledge improvement, pre- and post-training tests consisting of 20 multiple-choice questions (total score: 100 points) were administered. Statistical analyses, including independent sample t-tests and Wilcoxon signed-rank tests, were performed to evaluate intergroup differences in performance and learning outcomes.

Results

Both groups showed significant improvements in their post-training test scores, confirming the effectiveness of both teaching approaches. The AI group’s average score increased from 44.78 ± 8.9 to 51.98 ± 10.5, while the TR group’s score rose from 47.90 ± 9.8 to 60.90 ± 9.1. The TR group exhibited a greater improvement in average scores (t = -3.54, p = 0.00064) and demonstrated a narrower distribution of posttest scores, indicating superior performance in terms of knowledge enhancement and learning consistency. However, the AI group effectively reduced variability in pretest scores, suggesting its potential to promote equitable learning outcomes across participants with diverse baseline knowledge levels.

Conclusion

The Anesthesia Smart-AI model showed promise as a teaching aid, particularly in addressing variability in baseline knowledge and offering personalized learning pathways. However, traditional teaching methods were more effective in producing higher average scores and consistent learning outcomes. This suggests that while AI-based teaching holds potential for improving medical education, it may best serve as a complementary tool rather than a replacement for traditional methods. Future research should focus on optimizing AI-based educational tools to enhance their capabilities and integration into medical training programs. With continued development, AI-assisted teaching could offer scalable, innovative, and equitable approaches to address the growing demands of medical education. Acknowledgments: This research was supported by 2023 Wuxi Municipal Health Commission Youth Project: Application of the MED·SMART-AI Medical Artificial Intelligence Model in Clinical Practice and Teaching under Standardized Residency Training (Q202360); 2023 Nanjing Medical University Teaching Reform Project: Research on the Use of MED·SMART-AI for Teaching in Standardized Residency Training for Anesthesiology Residents (2023LX114) BEPS24074: APPLICATION OF H-UPPP SURGERY WITH PLASMA KNIFE UNDER GENERAL ANESTHESIA IN YOUNG AND MIDDLE-AGED FEMALE PATIENTS WITH OSAHS ACCOMPANIED BY COGNITIVE IMPAIRMENT Xiaomiao Lia, Lijuan Sunb aDepartment of Otolaryngology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China,bDepartment of Ophthalmology, Handan Aiyan Ophthalmology Hospital, Handan 056000, Hebei, China.

Background

To observe the application effect of modified uvulopalatopharyngoplasty (H-UPPP) with a plasma knife under general anesthesia in young and middle-aged female patients with obstructive sleep apnea hypopnea syndrome (OSAHS) accompanied by cognitive impairment.

Methods

A total of 106 OSAHS patients treated at Handan Aiyan Eye Hospital were selected and divided into an observation group and a control group, each consisting of 53 cases, using a random number table method. The control group received traditional behavioral intervention. The observation group was treated with the H-UPPP procedure. After treatment, sleep structure, cognitive function, memory and recall, P300 latency results, and the rate of abnormal electroencephalograms were compared between the two groups.

Results

There were no statistically significant differences in AHI, LSpo2, TS90%, (N1+N2) %, N3%, REM%, sleep latency, and sleep efficiency between the two groups before treatment (P>0.05). After treatment, the observation group showed reduced AHI, TS90%, and (N1+N2) % (P<0.05), increased N3%, LSpo2, and REM% (P0.05). In the control group after treatment, AHI decreased, and LSpo2 increased (P<0.05), with no changes in other indicators. The differences in AHI, LSpo2, TS90%, (N1+N2) %, N3%, and REM% between pre- and post-treatment in the observation group were statistically significant (p0.05). Before treatment, there were no statistically significant differences in MMSE and MOCA scores between the two groups (P>0.05). After treatment, both groups showed increased MMSE and MOCA scores, with the observation group being higher than the control group, and the difference was statistically significant (P0.05). After treatment, CFT, LMT, and their DR scores increased in both groups, but only LMT and LMT SR scores showed statistically significant differences, with the observation group being higher than the control group (P0.05). After treatment, Fz, Cz, Pz, C3, and C4 indices decreased in both groups, with the observation group being lower than the control group, and the difference was statistically significant (P<0.05). After treatment, the rate of abnormal electroencephalograms in the observation group was 22.64% (12/53) lower than that in the control group (49.06; 26/53), and the difference was statistically significant (P<0.05). Conclusion: The application of the plasma knife H-UPPP procedure under general anesthesia in young and middle-aged female patients with OSAHS accompanied by cognitive impairment can significantly improve sleep structure and cognitive impairment, making it worthy of clinical promotion and application. BEPS24075: PREDICTIVE VALUE OF P2PSA AND PROSTATE HEALTH INDEX IN DIAGNOSING PROSTATE CANCER USING TPSA 4-50 NG/ML Hewen Xua, Weiwang Caib,c,d aDepartment of General Medicine, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi 435000, Hubei, China,bMedical Laboratory, Second Hospital of Huangshi, Huangshi 435002, Hubei, China,cHuangshi Key Laboratory of Clinical Laboratory Technology, Huangshi 435000, Hubei, China,dHuangshi Yiyang Medical Laboratory, Huangshi 435000, Hubei, China. HX and WC contributed equally to this paper.

Background

The principal modality for the early diagnosis of prostate cancer hinges predominantly upon PSA screening. Prostate-specific antigen (PSA), a glycoprotein indigenous to prostate tissue, fluid, semen, serum, and urine and emanating from the epithelial linings of prostate acini and ducts, has been clinically implemented since the 1980s. It has substantially augmented the detection rate of prostate cancer (PCa) and has emerged as a ubiquitously employed screening protocol. However, in light of the propensity of PSA to exhibit abnormal elevations in patients with benign prostatic hyperplasia (BPH) and prostatitis, its role in surveilling the efficacy of early PCa screening and treatment remains a subject of intense debate. The limitations inherent in prostate cancer screening can precipitate a copious number of superfluous biopsies and overdiagnoses. Recently, several PSA isomers and derivatives, such as p2PSA and the prostate health index (PHI), have been unearthed and are esteemed to possess considerable clinical application merit. p2PSA and PHI can efficaciously augment the positive rate of needle biopsy, prognosticate high-grade prostate cancer, and appreciably curtail the incidence of unnecessary needle biopsies. The current research endeavored to evaluate the diagnostic utilities of p2PSA and PHI in prostate cancer screening within the tPSA range of 4 - 50 ng/mL. Subjects and Methods: A comprehensive investigation was carried out on 84 patients who underwent prostate aspiration procedures in three hospitals spanning from January 2021 to March 2022. The overarching objective of this study was to assess the diagnostic potency of prostate aspiration by meticulously measuring the levels of p2PSA, free prostate specific antigen (fPSA), and total prostate specific antigen (tPSA) and precisely calculating the PHI value. Patients with a prior history of prostate biopsy or electrodessication, those afflicted with urinary tract infections, urinary retention, bladder stones, or prostate massage within the preceding three months, as well as those currently ingesting medications such as 5ARIs and anti-androgens were precluded from the study. After scrupulously considering the aforementioned exclusion criteria, a total of 84 patients were ultimately enlisted in the final cohort.

Results

Among the 84 prostate perforations, 23 were diagnosed with prostate cancer, accounting for a proportion of 27.38%. The levels of tPSA, p2PSA, %p2PSA, and PHI were manifestly elevated in the tumor group as compared to the non-tumor group, whereas the levels of fPSA/tPSA and %fPSA were comparatively lower. The AUC (area under the ROC curve) values for tPSA, fPSA, fPSA/tPSA, %fPSA, p2PSA, %p2PSA, and PHI were 0.7306, 0.5456, 0.7466, 0.7441, 0.8346, 0.7705, and 0.8432 respectively. Among all the parameters, PHI and p2PSA evinced the most preponderant diagnostic value, %p2PSA and PHI demonstrated the most optimal sensitivity performance, and %p2PSA and %fPSA exhibited the most sterling specificity performance.

Conclusions

p2PSA and PHI manifested more propitious diagnostic values among prostate cancer patients. If p2PSA and PHI were applied to this patient assemblage, a diminution in the number of perforations could be effected.