Characterizing Early Monoarthritis in Rats: A Pathological and Clinical Study of an Mbsa/cfa-induced Model

Characterizing Early Monoarthritis in Rats: A Pathological and Clinical Study of an Mbsa/cfa-induced Model | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Characterizing Early Monoarthritis in Rats: A Pathological and Clinical Study of an Mbsa/cfa-induced Model Marco Aurélio A. Pereira, Lizandre Keren R. da Silveira, Paula C. Ferian, and 8 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7697597/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 12 You are reading this latest preprint version Abstract A comprehensive understanding of arthritis pathophysiology, involving inflammation, pain, and joint complications, is crucial for enhancing diagnostic accuracy and therapeutic approaches. This study examines the inflammatory mechanisms and clinical implications of arthritis. Male Wistar rats were subjected to arthritis induction in the right knee via intra-articular injection of mBSA/CFA. They were divided into a control group (CG, n = 10) and two arthritis groups (8-day – IG and 13-day – AG, n = 8 each) treated with tramadol at 17,8 mg/kg SC q.8 h. Histological and immunohistochemical analyses revealed an influx of inflammatory cells, mainly CD68 + macrophages. [18F] FDG PET/CT revealed increased intra-articular glucose metabolism. The data were analyzed via t tests, two-way ANOVA or two-way repeated-measures ANOVA, and Mann‒Whitney, Kruskal‒Wallis, or Friedman tests (p < 0.05). Both arthritis groups presented increased PGE 2 and collagen I and V in the right knee, with clinical signs such as reduced weight bearing, weight loss, and increased pain, indicating inflammatory arthritis. During testing, the control group presented decreased movement and velocity but a greater rearing frequency than did the AG group. This study clarifies arthritis inflammation and affirms the importance of animal models in the development of better treatments. These findings also substantiate the efficacy of tramadol in alleviating arthritis-associated pain. Health sciences/Diseases Biological sciences/Immunology Health sciences/Medical research Health sciences/Rheumatology synovitis inflammatory arthritis pain PET/CT prostaglandin E2 Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Revision requested 16 Nov, 2025 Reviews received at journal 12 Nov, 2025 Reviews received at journal 11 Nov, 2025 Reviews received at journal 06 Nov, 2025 Reviewers agreed at journal 23 Oct, 2025 Reviewers agreed at journal 20 Oct, 2025 Reviewers agreed at journal 14 Oct, 2025 Reviewers invited by journal 14 Oct, 2025 Editor assigned by journal 14 Oct, 2025 Editor invited by journal 07 Oct, 2025 Submission checks completed at journal 03 Oct, 2025 First submitted to journal 03 Oct, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7697597","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":535015702,"identity":"517a56e0-12b6-43c7-bf27-18941a592c4b","order_by":0,"name":"Marco Aurélio A. 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Model\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"synovitis, inflammatory arthritis, pain, PET/CT, prostaglandin E2","lastPublishedDoi":"10.21203/rs.3.rs-7697597/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7697597/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eA comprehensive understanding of arthritis pathophysiology, involving inflammation, pain, and joint complications, is crucial for enhancing diagnostic accuracy and therapeutic approaches. This study examines the inflammatory mechanisms and clinical implications of arthritis. Male Wistar rats were subjected to arthritis induction in the right knee via intra-articular injection of mBSA/CFA. They were divided into a control group (CG, n\u0026thinsp;=\u0026thinsp;10) and two arthritis groups (8-day \u0026ndash; IG and 13-day \u0026ndash; AG, n\u0026thinsp;=\u0026thinsp;8 each) treated with tramadol at 17,8 mg/kg SC q.8 h. Histological and immunohistochemical analyses revealed an influx of inflammatory cells, mainly CD68\u0026thinsp;+\u0026thinsp;macrophages. [18F] FDG PET/CT revealed increased intra-articular glucose metabolism. The data were analyzed via t tests, two-way ANOVA or two-way repeated-measures ANOVA, and Mann‒Whitney, Kruskal‒Wallis, or Friedman tests (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05). Both arthritis groups presented increased PGE\u003csub\u003e2\u003c/sub\u003e and collagen I and V in the right knee, with clinical signs such as reduced weight bearing, weight loss, and increased pain, indicating inflammatory arthritis. During testing, the control group presented decreased movement and velocity but a greater rearing frequency than did the AG group. This study clarifies arthritis inflammation and affirms the importance of animal models in the development of better treatments. 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