Crop rotation alleviates continuous cropping obstacles in Chrysanthemum morifolium production by regulating rhizosphere microbial communities and metabolites

Crop rotation alleviates continuous cropping obstacles in Chrysanthemum morifolium production by regulating rhizosphere microbial communities and metabolites | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Crop rotation alleviates continuous cropping obstacles in Chrysanthemum morifolium production by regulating rhizosphere microbial communities and metabolites Qiaohuan Chen, Jinxin Li, Xiao Wang, Zhiyuan Liao, Siqian Xiao, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4395851/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Chrysanthemum morifolium is widely used as a raw material in traditional Chinese medicine and in health drinks. However, continuous cropping obstacles have produced bottlenecks in C. morifolium production. Crop rotation is considered to be the cheapest and most effective method to restore soil quality. Therefore, we here studied the impact of two crop rotation models ( Prunella vulgaris - C. morifolium and Brassica napus - C. morifolium ) on C. morifolium productivity and soil quality. Our results showed that both rotation models led to a higher C. morifolium yield and quality advantage than monoculture model; subsequently, the main reasons for improving C. morifolium productivity by these two crop rotation modes were analyzed. Levels of soil nutrients, such as total nitrogen, available phosphorus, and available iron, showed significant augment in crop rotation; similar situation were observed for the activities of soil acid phosphatase, sucrase, and β-glucosidase. Furthermore, the reduced wilt disease incidence and the decreased Fusarium pathogen adundance under rotation could be attributed to the changes in bacterial community structure and rhizosphere metabolites. In addition, changes in the abundance of metabolites in the rhizosphere soil were key factors affecting the bacterial and fungal community structure. For instance, two rotation systems decreased the presence of Proteobacteria and increased other bacterial phyla (e.g. Actinobacteria, Acidobacteriota, Cyanobacteria), potentially influenced by rhizosphere soil metabolites. This study offers new insights into the mechanisms by which continuous cropping challenges can be overcome and provides concrete suggestions for improving yield of an economically and medicinally important plant species. Chrysanthemum morifolium Cropping system Fusarium wilt Rhizosphere microorganisms Rhizosphere metabolites Full Text Supplementary Files FigS1and2.docx TableS1.xlsx TableS2.xlsx TableS3.xlsx TableS4.xlsx TableS5.xlsx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4395851","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":308249906,"identity":"9a77037a-866b-4b01-ac58-a71f0d5a8cb2","order_by":0,"name":"Qiaohuan Chen","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA1UlEQVRIie3RMQrCMBTG8VcicQl2jQj1BMKTglKQepVKoVMHRzcDXT1CwSsIgnOwEJd27yCICJ066GwHV50aN8H89x+8jwdgMv1gtJucng3OyFxkUo/0mAo4W0ZdtFSgRxweI2f3o40kR83DIEbkKAdTmj/KGnxnJFqJWgaIZ9fbFHsvhdCdyDZiJTsZYBVCWRwGDOTi0EoIjIXEbC0udaVJaMe1BGYEZU41CaMRAYxIXyjXS1Fjy3B7UwSaGbEhu5b1yndayUecab7mnXwrTCaT6S96AYadQof5yu6lAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-6315-3835","institution":"Hubei University of Chinese Medicine","correspondingAuthor":true,"prefix":"","firstName":"Qiaohuan","middleName":"","lastName":"Chen","suffix":""},{"id":308249907,"identity":"f425c898-f9c6-4503-9b17-ed826e7588ed","order_by":1,"name":"Jinxin Li","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Jinxin","middleName":"","lastName":"Li","suffix":""},{"id":308249908,"identity":"f14fab9f-b096-438c-8095-cfe60a433339","order_by":2,"name":"Xiao Wang","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Xiao","middleName":"","lastName":"Wang","suffix":""},{"id":308249909,"identity":"380ef678-5dea-4bc2-aaf7-cb30f55f3ccc","order_by":3,"name":"Zhiyuan Liao","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Zhiyuan","middleName":"","lastName":"Liao","suffix":""},{"id":308249910,"identity":"ea3b43a3-2893-42fd-b99c-b606d5491ab3","order_by":4,"name":"Siqian Xiao","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Siqian","middleName":"","lastName":"Xiao","suffix":""},{"id":308249911,"identity":"bfac86d1-32be-40b2-a5be-70daebbb8143","order_by":5,"name":"Yuhuan Miao","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Yuhuan","middleName":"","lastName":"Miao","suffix":""},{"id":308249912,"identity":"bb46c1ef-e80c-459f-9bec-2c240e95c9c3","order_by":6,"name":"Dahui Liu","email":"","orcid":"https://orcid.org/0000-0001-6518-9068","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Dahui","middleName":"","lastName":"Liu","suffix":""}],"badges":[],"createdAt":"2024-05-09 14:40:15","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4395851/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4395851/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":59391659,"identity":"0f96a81b-32fb-4490-ae2f-b5b31f047e1a","added_by":"auto","created_at":"2024-07-01 08:14:41","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1316813,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4395851/v1_covered_83c12dba-f9ea-4f29-8254-a6f8fff4f10c.pdf"},{"id":58141689,"identity":"a3a718e6-3962-477e-baf7-6fc5bea287cc","added_by":"auto","created_at":"2024-06-11 17:48:58","extension":"docx","order_by":5,"title":"","display":"","copyAsset":false,"role":"supplement","size":266306,"visible":true,"origin":"","legend":"","description":"","filename":"FigS1and2.docx","url":"https://assets-eu.researchsquare.com/files/rs-4395851/v1/2795a3b5b5185d07c562639a.docx"},{"id":58141684,"identity":"ef9d1207-ffbd-4821-8eaf-64796bc034fa","added_by":"auto","created_at":"2024-06-11 17:48:58","extension":"xlsx","order_by":6,"title":"","display":"","copyAsset":false,"role":"supplement","size":11099,"visible":true,"origin":"","legend":"","description":"","filename":"TableS1.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-4395851/v1/238af31ccce1bb8818f05e0a.xlsx"},{"id":58141685,"identity":"39830179-c212-4053-b32a-f6dbdbe556d2","added_by":"auto","created_at":"2024-06-11 17:48:58","extension":"xlsx","order_by":7,"title":"","display":"","copyAsset":false,"role":"supplement","size":45010,"visible":true,"origin":"","legend":"","description":"","filename":"TableS2.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-4395851/v1/6340e3c6f2b930b36026c010.xlsx"},{"id":58142015,"identity":"3ed7dcb0-e256-43ae-98b7-eafe479f78e7","added_by":"auto","created_at":"2024-06-11 17:56:58","extension":"xlsx","order_by":8,"title":"","display":"","copyAsset":false,"role":"supplement","size":88207,"visible":true,"origin":"","legend":"","description":"","filename":"TableS3.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-4395851/v1/44111685feab7500248e16e0.xlsx"},{"id":58142456,"identity":"b1dfc33b-2947-40f3-8975-bfe7b7ea772f","added_by":"auto","created_at":"2024-06-11 18:04:58","extension":"xlsx","order_by":9,"title":"","display":"","copyAsset":false,"role":"supplement","size":10053,"visible":true,"origin":"","legend":"","description":"","filename":"TableS4.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-4395851/v1/9a855936804e24712e464593.xlsx"},{"id":58141686,"identity":"bc636e13-333c-44fa-8250-18d6bee2da5f","added_by":"auto","created_at":"2024-06-11 17:48:58","extension":"xlsx","order_by":10,"title":"","display":"","copyAsset":false,"role":"supplement","size":37538,"visible":true,"origin":"","legend":"","description":"","filename":"TableS5.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-4395851/v1/3a31c601628a5074823eca0d.xlsx"}],"financialInterests":"","formattedTitle":"Crop rotation alleviates continuous cropping obstacles in Chrysanthemum morifolium production by regulating rhizosphere microbial communities and metabolites","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Chrysanthemum morifolium, Cropping system, Fusarium wilt, Rhizosphere microorganisms, Rhizosphere metabolites","lastPublishedDoi":"10.21203/rs.3.rs-4395851/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4395851/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e \u003cem\u003eChrysanthemum morifolium\u003c/em\u003e is widely used as a raw material in traditional Chinese medicine and in health drinks. However, continuous cropping obstacles have produced bottlenecks in \u003cem\u003eC. morifolium\u003c/em\u003e production. Crop rotation is considered to be the cheapest and most effective method to restore soil quality. Therefore, we here studied the impact of two crop rotation models (\u003cem\u003ePrunella vulgaris\u003c/em\u003e-\u003cem\u003eC. morifolium\u003c/em\u003e and \u003cem\u003eBrassica napus\u003c/em\u003e-\u003cem\u003eC. morifolium\u003c/em\u003e) on \u003cem\u003eC. morifolium\u003c/em\u003e productivity and soil quality. Our results showed that both rotation models led to a higher \u003cem\u003eC. morifolium\u003c/em\u003e yield and quality advantage than monoculture model; subsequently, the main reasons for improving \u003cem\u003eC. morifolium\u003c/em\u003e productivity by these two crop rotation modes were analyzed. Levels of soil nutrients, such as total nitrogen, available phosphorus, and available iron, showed significant augment in crop rotation; similar situation were observed for the activities of soil acid phosphatase, sucrase, and β-glucosidase. Furthermore, the reduced wilt disease incidence and the decreased \u003cem\u003eFusarium\u003c/em\u003e pathogen adundance under rotation could be attributed to the changes in bacterial community structure and rhizosphere metabolites. In addition, changes in the abundance of metabolites in the rhizosphere soil were key factors affecting the bacterial and fungal community structure. For instance, two rotation systems decreased the presence of Proteobacteria and increased other bacterial phyla (e.g. Actinobacteria, Acidobacteriota, Cyanobacteria), potentially influenced by rhizosphere soil metabolites. This study offers new insights into the mechanisms by which continuous cropping challenges can be overcome and provides concrete suggestions for improving yield of an economically and medicinally important plant species.\u003c/p\u003e","manuscriptTitle":"Crop rotation alleviates continuous cropping obstacles in Chrysanthemum morifolium production by regulating rhizosphere microbial communities and metabolites","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-06-11 17:48:54","doi":"10.21203/rs.3.rs-4395851/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"9a37f970-2c7c-45ed-9041-dbafaf01601b","owner":[],"postedDate":"June 11th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-07-01T08:06:33+00:00","versionOfRecord":[],"versionCreatedAt":"2024-06-11 17:48:54","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4395851","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4395851","identity":"rs-4395851","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}