Green walnut husk extracts restrain proliferation and invasion of endometrial stromal cells by inhibiting the PI3K/AKT signaling pathway

This study investigates the effects of green walnut husk (gwh) extracts on endometrial stromal cells (escs) and the pi3k/akt signaling pathway. endometrial tissues were collected frompatients with endometriosis (ems) and escs wereisolated. the effects of gwh extracts—low (30 μg/mL),medium (100 μg/mL), and high (200 μg/mL)—on escs proliferation, migration, and invasion were examined using cck-8, edu, and transwell assays. the transcription levels and protein expressions of pi3k/akt were detected. an ems mouse model was established to assess the growth of ectopic endometrial lesions. gwh extracts exerted a significant inhibitory effect on escs proliferation, migration, and invasion. similarly, the extracts demonstrated suppression of pi3k and akt level in escs. in the ems mouse model, gwh extracts displayed an inhibitory impact on the growth of ectopic endometrial lesions. our study demonstrated that gwh extracts restrained the proliferative and invasive capabilities of escs, thereby alleviating ems.