Feasibility of integrated backscatter for quantitative diagnosis between focus of adenomyosis and normal myometrium in vitro

Objective To study the feasibility of integrated backscatter(IBS) for quantitative differential diagnosis between the normal myometrium and the focus of adenomyosis in vitro.Methods Two-dimension and IBS scanning were performed immediately on fresh myometrium specimens after being excised.The imaging of sections were kept into archives,then used for the values of average image intensity(AII) by the analysis programs of the same imaging system.The specimens were fixed immediately after ultrasound scanning,then were embedded by paraffin,sliced and stained by haematoxylin-eosin in the routine way.After the slices were read by senior pathologist,the calibrated average image intensity(AII%) of IBS imaging was divided into two groups as the normal myometrium and the adenomyosis according to their pathological diagnosis.Results Among the specimens,32 cases were diagnosed as the focus of adenomyosis and 34 cases as the normal myometrium by pathologic examination.There was no significant difference between the average age of patients of two groups(P(0.05));However,there was significant difference between the AII% of the normal myometrium and the focus of adenomyosis,which were(3.71)±(0.57) and(3.00)±(0.33) respectively.Conclusions It is feasible to use IBS for quantitative diagnosis between the normal myometrium and the focus of adenomyosis in vitro,and it is potential to apply this technique in clinical ultrasound diagnosis of gynecology.