Establishment and Evaluation of Rat Models with Qi Stagnancy and Blood Stasis Symptom by Endometriosis

Objective: Using integration of multi-factor approach to establish the qi stagnancy and blood stasis endometriosis model of rats.Provide appropriate models for in-depth study of pathological mechanisms and TCM treatment of endometriosis.Methods: Based on the duplication of the rat models of endometriosis by endometrial tissue autologous transplant surgery,we used integration of multi-factor approach,such as drugs and emotional stimulation to copy the qi stagnancy and blood stasis rat models.Evaluated the rat models by observing the external and behavioral performance,tissue morphologic features of the reign and ectopic endometrial tissue,and the changes of vascular endothelial factor and monoamine neurotransmitter.Results:(1)The effective rate of the rat modeling was 78%.(2)Disease and syndrome combination group was higher in NO than that in model group and Sham-operated group,and both groups were significantly different(P0.05).Disease and syndrome combination group was higher in ET than that in model group,and both groups were significantly different(P0.05).(3)Compared with disease model group,disease and syndrome combination group was significantly higher in serum NE,DA,and both groups were significantly different(P0.05).Conclusion: The Qi stagnancy and blood stasis endometriosis model,which is established by integration of multi-factor approach,is basically consistent with both TCM syndrome features and pathological features of Endometriosis.