Functional study of eIF5A in Nicotiana tabacum by Riobosome-seq

Functional study of eIF5A in Nicotiana tabacum by Riobosome-seq | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Functional study of eIF5A in Nicotiana tabacum by Riobosome-seq Liangquan Wang, Ying Wang, Yongbo Qu, Xinxi He, Yu Wu, Shuaibin Wang, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9146978/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Tobacco serves as a crucial economic crop globally, having significantly propelled the development of human society. In this study, Nicotiana tabacum was employed as the research model to explore the potential biological functions of 10 genes of the eIF5A family, which contributes to an in-depth understanding of the potential gene redundancy phenomenon. As a eukaryotic translation initiation factor, eIF5A exhibits high conservation across plant species, including N. tabacum . A total of 1,019 differentially expressed genes (DEGs) were identified in, among which 656 were upregulated and 363 downregulated specifically at the translational level, rather than the transcriptional level. The eif5a mut exhibits extremely obvious phenotypic characteristics and is shorter than the wild type(K326). Through Gene Ontology (GO) and KEGG enrichment analyses of these DEGs, they were found to be significantly enriched in photosynthesis-related pathways, including roles in light capture and the composition of the photosynthetic system. This finding is essential for understanding the roles of the eIF5A gene and its hypusination modification in plant development and stress responses. Additionally, prediction of the protein-protein interaction (PPI) network of eIF5A revealed potential interactions with proteins such as DHS1, suggesting its involvement in Deoxyhypusine synthesis via PPI. This study systematically explores the biological functions of eIF5A in N. tabacum , filling a research gap in the current understanding of eIF5A in tobacco. The findings lay a foundation for further investigations aimed at improving tobacco quality. Nicotiana tabacum eIF5A Riobosome-seq translation Full Text Additional Declarations No competing interests reported. Figures are available in the Supplementary Files section. Supplementary Files SupplementaryInformation.pdf Figures.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9146978","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":617925036,"identity":"7af4e570-3163-464e-b823-c7ba8d75831f","order_by":0,"name":"Liangquan Wang","email":"","orcid":"","institution":"Technology Center,China Tobacco Hunan Industrial Co., Ltd L","correspondingAuthor":false,"prefix":"","firstName":"Liangquan","middleName":"","lastName":"Wang","suffix":""},{"id":617925037,"identity":"af46b8b8-7551-4377-98d9-fdd194c6e043","order_by":1,"name":"Ying Wang","email":"","orcid":"","institution":"Hunan 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In this study, \u003cem\u003eNicotiana tabacum\u003c/em\u003e was employed as the research model to explore the potential biological functions of 10 genes of the eIF5A family, which contributes to an in-depth understanding of the potential gene redundancy phenomenon. As a eukaryotic translation initiation factor, eIF5A exhibits high conservation across plant species, including \u003cem\u003eN. tabacum\u003c/em\u003e. A total of 1,019 differentially expressed genes (DEGs) were identified in, among which 656 were upregulated and 363 downregulated specifically at the translational level, rather than the transcriptional level. The \u003cem\u003eeif5a\u003c/em\u003e\u003csup\u003e\u003cem\u003emut\u003c/em\u003e\u003c/sup\u003e exhibits extremely obvious phenotypic characteristics and is shorter than the wild type(K326). 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