A Curcumin derivative CU1 loaded long-circulating pH sensitive nanoliposome for the treatment of lung cancer: in vitro and vivo evaluation.

A Curcumin derivative CU1 loaded long-circulating pH sensitive nanoliposome for the treatment of lung cancer: in vitro and vivo evaluation. | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 19 July 2025 V1 Latest version Share on A Curcumin derivative CU1 loaded long-circulating pH sensitive nanoliposome for the treatment of lung cancer: in vitro and vivo evaluation. Authors : Ling Zhao [email protected] , Yuqian Pu , Peng Shi , Yumeng Wei , Xinya Li , Ke Li , Yongqiang Jiang , Qiong Zhang , Chao Pi , and Hongru Yang Authors Info & Affiliations https://doi.org/10.22541/au.175291040.04054288/v1 137 views 56 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background and Purpose: CU1, a curcumin derivative developed by our team, has been shown to have stronger anti-lung cancer effects than curcumin. However, CU1’s poor water solubility and bioavailability limit its further application. This study aims to improve the efficacy and safety of CU1. Experimental Approach: The long-circulating acid-sensitive nanoliposomes loaded CU1 (CU1-LCpHL) were prepared using DOPE, CHEMS and DSPE-PEG2000 as carriers, and evaluated in vitro and in vivo. Key Results: CU1-LCpHL has a spherical shape with uniform particle size, remains stable after 3-month lyophilisation at 25°C, and shows slow drug release. Compared with CU, CU1, and CU1-LSLN, it significantly enhances lung cancer cell uptake and apoptosis induction, more effectively inhibits their proliferation and migration, and has a 9.52/9.47-fold higher AUC0-t and 3.37/7.69-fold longer MRT0-t than CU/CU1. Its lung cancer inhibitory effect is 2.42/2.17/1.37 times that of CU/CU1/CU1-LSLN, with no significant organ toxicity. Mechanistically, it upregulates Caspase-3, Caspase-9, and Bax, and downregulates p-AKT/AKT and Bcl-2 (confirmed by WB based on network pharmacology). Immunohistochemistry shows Ki67, CD34, and VEGFR2 positive rates in the order: CU1-LCpHL < CU1-LSLN < CU1 < CU. Conclusion and Implications: The enhanced anti-lung cancer effect of CU1-LCpHL may involve the induction of apoptosis in lung cancer cells through the upregulation of pro-apoptotic proteins and downregulation of anti-apoptotic proteins. CU1-LCpHL is a highly promising lung cancer treatment delivery system. Supplementary Material File (manuscript.docx) Download 2.60 MB Information & Authors Information Version history V1 Version 1 19 July 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Authors Affiliations Ling Zhao [email protected] Southwest Medical University View all articles by this author Yuqian Pu Southwest Medical University View all articles by this author Peng Shi Southwest Medical University View all articles by this author Yumeng Wei Southwest Medical University View all articles by this author Xinya Li Southwest Medical University View all articles by this author Ke Li Southwest Medical University View all articles by this author Yongqiang Jiang Southwest Medical University View all articles by this author Qiong Zhang Southwest Medical University View all articles by this author Chao Pi Southwest Medical University View all articles by this author Hongru Yang The Fourth Affiliated Hospital, Southwest Medical University (Tianfu Affiliated Hospital), No. 168, Huantianfu New Area Expressway, Meishan Tianfu New Area, Meishan City, Sichuan, 620564, P.R. China. View all articles by this author Metrics & Citations Metrics Article Usage 137 views 56 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Ling Zhao, Yuqian Pu, Peng Shi, et al. A Curcumin derivative CU1 loaded long-circulating pH sensitive nanoliposome for the treatment of lung cancer: in vitro and vivo evaluation.. Authorea . 19 July 2025. DOI: https://doi.org/10.22541/au.175291040.04054288/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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