Preliminary Study on Human Adipose Stem Cells Promoting Skin Wound Healing Through Notch1 Signaling Pathway

Background: Mesenchymal stem cells (MSCs) have been documented as possible candidates for wound healing treatment because their use could reinforce the regenerative capacity of many tissues. Human adipose stem cells (hADSCs) have the advantages of easy access, large quantity and easy operation. They can be fully applied in the treatment of skin wounds. In this study, we aim to explore the roles and potential mechanisms of hADSCs in cutaneous wound healing. Methods: hADSCs were obtained from human subcutaneous fat. Adipocytes and osteocytes differentiated from hADSCs were determined by staining with Oil Red O and alkaline phosphatase (ALP), respectively. We assessed the effects of hADSCs and hADSC conditional medium (CM) on wound healing in an injury model of mice. Than, we investigated the biological effects of hADSCs on human keratinocytes HaCAT cells in vitro. Results: The results showed that hADSCs could be successfully differentiated into osteogenic and lipogenic cells. hADSCs and hADSCs-CM significantly promote skin wound healing in vivo. hADSCs significantly promoted HaCAT cells proliferation and migration through activating Notch1 signaling pathway, and activated the AKT signaling pathway by Rps6kb1 kinase in HaCAT cells. In addition, we found that hADSCs-mediated activation of Rps6kb1/AKT signaling was dependent on the Notch1 signaling pathway. Conclusion: We demonstrated that hADSCs can promote skin cell-HaCAT cells proliferation and migration via Notch1 pathway, suggesting that hADSCs may provide an alternative therapeutic approach for the treatment of skin injury.