L-Carnitine Functionalized Iron oxide (LC@Fe₃O₄) Nanocomposites: Synthesis, Characterization, and Applications in Antibacterial and Anticancer

L-Carnitine Functionalized Iron oxide (LC@Fe₃O₄) Nanocomposites: Synthesis, Characterization, and Applications in Antibacterial and Anticancer | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article L-Carnitine Functionalized Iron oxide (LC@Fe₃O₄) Nanocomposites: Synthesis, Characterization, and Applications in Antibacterial and Anticancer Mariyappan Yogeshwari, Dhanasekaran Suraksha, Devendiran Parthiban, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7775485/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 21 Feb, 2026 Read the published version in BioNanoScience → Version 1 posted 12 You are reading this latest preprint version Abstract In this study, a multifunctional nanocomposite was prepared by integrating core–shell structured Fe₃O₄ nanoparticles with a layer of L-carnitine (LC), designed to improve biocompatibility, magnetic responsiveness, and antibacterial activity for biomedical use. FTIR analysis confirmed successful pairing of L-Carnitine through characteristic functional groups, while XRD revealed a highly crystalline inverse spinel structure of Fe₃O₄. SEM and TEM images demonstrated uniform spherical particles featuring a Fe₃O₄ enclosed within a distinct L-carnitine layer. TGA indicated thermal stability and infrered the presence of organic content, supporting effective surface modification. VSM analysis confirmed super paramagnetism with decreased magnetic saturation due to the LC coating. The prepared nano-composite was examined for anti-bacterial activity, and the LC@Fe₃O₄ nanocomposite showed strong antibacterial activity against both positive and negative bacterial strains. Cytotoxicity assays on MDA-MB-231 cells confirmed excellent anti-cancer properties. Fe₃O₄ L-Carnitine Antibacterial Cytotoxicity Nano-composite Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 21 Feb, 2026 Read the published version in BioNanoScience → Version 1 posted Editorial decision: Revision requested 10 Nov, 2025 Reviews received at journal 26 Oct, 2025 Reviewers agreed at journal 26 Oct, 2025 Reviews received at journal 24 Oct, 2025 Reviews received at journal 22 Oct, 2025 Reviewers agreed at journal 14 Oct, 2025 Reviewers agreed at journal 14 Oct, 2025 Reviewers agreed at journal 14 Oct, 2025 Reviewers invited by journal 14 Oct, 2025 Editor assigned by journal 14 Oct, 2025 Submission checks completed at journal 13 Oct, 2025 First submitted to journal 03 Oct, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7775485","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":534564090,"identity":"d722bf7a-ae56-4b38-9dfe-bdd8f132adb3","order_by":0,"name":"Mariyappan Yogeshwari","email":"","orcid":"","institution":"Rajeswari Vedachalam Government Arts College","correspondingAuthor":false,"prefix":"","firstName":"Mariyappan","middleName":"","lastName":"Yogeshwari","suffix":""},{"id":534564091,"identity":"86254a33-2967-44c1-aa89-29e34b1047b2","order_by":1,"name":"Dhanasekaran Suraksha","email":"","orcid":"","institution":"Rajeswari Vedachalam 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