Synergistic Defence: Electron Beam Irradiated Chitosan and Bacillus subtilis sp. in Combating Chickpea Wilt for sustainable agriculture

Synergistic Defence: Electron Beam Irradiated Chitosan and Bacillus subtilis sp. in Combating Chickpea Wilt for sustainable agriculture | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Synergistic Defence: Electron Beam Irradiated Chitosan and Bacillus subtilis sp. in Combating Chickpea Wilt for sustainable agriculture Sravani Bana, T. K. Narute, S. B. Latke, R. M. Naik, S. V. Kolse, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6236890/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background In response to the pressing global demand for sustainable management practices against Fusarium wilt, a devastating disease in chickpeas, we present a groundbreaking approach: the fusion of nanotechnology with bio-control agents. By integrating nanotechnology with Bacillus subtilis as bio-control agents, we offer a cutting-edge strategy for mitigating wilt incidence while fostering agricultural sustainability. The application of irradiated chitosan at 150 ppm enhances the induction of antioxidant metabolites, thereby promoting the growth and yield of chickpeas. Results The antifungal activity of irradiated chitosan and Bacillus sp. resulted in the induction of different antifungal metabolites that augmented an immunomodulating role inhibiting the growth of the pathogen. As compared to the non-treated (37.3%) a lower wilt occurrence (8.34%), and 50.52% higher productivity was noticed in treated chickpeas. This dual strategy not only boosts plant growth and yield but also represents an innovative phytosanitary measure against Fusarium wilt and a sustainable approach to managing climate change adversities in chickpeas. Conclusions This approach of harnessing the strength of nano-biotechnology, holds promise for reducing reliance on pesticides, overuse of chemical fertilizers, thereby promoting eco-friendly and economically viable chickpea production practices mitigating climate change. Chickpea Irradiated Chitosan Induced Disease Resistance Wilt Sustainability Full Text Additional Declarations No competing interests reported. Supplementary Files Supplimentarydata.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6236890","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":441604194,"identity":"7a2c5e82-8225-419d-83f6-e2fe1e9cc283","order_by":0,"name":"Sravani Bana","email":"","orcid":"","institution":"Mahatma Phule Krishi Vidyapeeth","correspondingAuthor":false,"prefix":"","firstName":"Sravani","middleName":"","lastName":"Bana","suffix":""},{"id":441604197,"identity":"44b7bb87-1dc5-445e-8e65-3082066658e8","order_by":1,"name":"T. K. 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