Potential role of SIRT-1 and SIRT-3 as biomarkers for the diagnosis and prognosis of idiopathic pulmonary fibrosis

Potential role of SIRT-1 and SIRT-3 as biomarkers for the diagnosis and prognosis of idiopathic pulmonary fibrosis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Potential role of SIRT-1 and SIRT-3 as biomarkers for the diagnosis and prognosis of idiopathic pulmonary fibrosis Fabio Perrotta, Vito D’Agnano, Domenica Francesca Mariniello, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4046708/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 26 Apr, 2024 Read the published version in Respiratory Research → Version 1 posted 7 You are reading this latest preprint version Abstract Background : Idiopathic pulmonary fibrosis (IPF) is a debilitating and progressive lung disease of unknown aetiology, characterized by the relentless deposition of fibrotic tissue. Biomarkers may play a pivotal role as indicators of disease presence, progression, and treatment response. Sirtuins, a family of enzymes with ADP ribosyltransferase or deacetylase activity, have been implicated in several diseases, including pulmonary fibrosis. Methods : A cross-sectional, prospective, observational single-center study was conducted to investigate the potential role of serum SIRTs levels as biomarkers in patients with IPF. Demographic, clinical, and functional data and serological samples were collected from 34 patients with IPF followed at the Interstital Lung and Rare Diseases Outpatient Clinic of the Vanvitelli Pneumology Clinic, Monaldi Hospital, Naples, Italy and from 19 age-matched controls. Results : Serum SIRT-1 levels were significantly reduced in IPF patients compared to controls (median IPF 667 [435-858] pg/mL versus controls 925 [794-1173] pg/mL; p<.001 ). In contrast, serum SIRT-3 levels were significantly increased in IPF patients compared to controls (median IPF 338 [230-500] pg/mL versus controls 154 [99.8-246] pg/mL; p<.001). There were no statistically significant differences in serum SIRT-6 and SIRT-7 levels between IPF and controls. In addition, we found a significant positive correlation between SIRT-1 and lung function parameters such as FEV 1 % (ϱ=0.417;p=0.016), FVC% (ϱ=0.449;p=0.009) and DL CO % (ϱ=0.393;p=0.024), while a significant negative correlation was demonstrated between SIR-1 and GAP score, demonstrating a significant reduction in SIRT-1 in advanced Gender-Age-Physiology (GAP) stages 2-3 compared to GAP stage 1 (p=0.008). Conclusions : This prospective, cross-sectional study showed that SIRT-1 was associated with lung function and IPF severity and that both SIRT-1 and SIRT-3 could be considered as potential biomarkers of IPF, whereas SIRT-6 and SIRT-7 were not associated with IPF. Idiopathic pulmonary fibrosis Sirtuins Biomarkers Senescence Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 26 Apr, 2024 Read the published version in Respiratory Research → Version 1 posted Editorial decision: Accepted 30 Mar, 2024 Reviews received at journal 30 Mar, 2024 Reviewers agreed at journal 11 Mar, 2024 Reviewers invited by journal 11 Mar, 2024 Editor assigned by journal 11 Mar, 2024 Submission checks completed at journal 11 Mar, 2024 First submitted to journal 08 Mar, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4046708","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":278478107,"identity":"cbab6d11-bb8f-4865-890e-244e2c37886a","order_by":0,"name":"Fabio Perrotta","email":"data:image/png;base64,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","orcid":"","institution":"University of Campania ‘L. 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Biomarkers may play a pivotal role as indicators of disease presence, progression, and treatment response. Sirtuins, a family of enzymes with ADP ribosyltransferase or deacetylase activity, have been implicated in several diseases, including pulmonary fibrosis.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e: A cross-sectional, prospective, observational single-center study was conducted to investigate the potential role of serum SIRTs levels as biomarkers in patients with IPF. Demographic, clinical, and functional data and serological samples were collected from 34 patients with IPF followed at the Interstital Lung and Rare Diseases Outpatient Clinic of the Vanvitelli Pneumology Clinic, Monaldi Hospital, Naples, Italy and from 19 age-matched controls.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e: Serum SIRT-1 levels were significantly reduced in IPF patients compared to controls (median IPF 667 [435-858] pg/mL versus controls 925 [794-1173] pg/mL; p\u0026lt;.001 ). In contrast, serum SIRT-3 levels were significantly increased in IPF patients compared to controls (median IPF 338 [230-500] pg/mL versus controls 154 [99.8-246] pg/mL; p\u0026lt;.001). There were no statistically significant differences in serum SIRT-6 and SIRT-7 levels between IPF and controls. In addition, we found a significant positive correlation between SIRT-1 and lung function parameters such as FEV\u003csub\u003e1\u003c/sub\u003e% (ϱ=0.417;p=0.016), FVC% (ϱ=0.449;p=0.009) and DL\u003csub\u003eCO\u003c/sub\u003e% (ϱ=0.393;p=0.024), while a significant negative correlation was demonstrated between SIR-1 and GAP score, demonstrating a significant reduction in SIRT-1 in advanced Gender-Age-Physiology (GAP) stages 2-3 compared to GAP stage 1 (p=0.008).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusions\u003c/strong\u003e: This prospective, cross-sectional study showed that SIRT-1 was associated with lung function and IPF severity and that both SIRT-1 and SIRT-3 could be considered as potential biomarkers of IPF, whereas SIRT-6 and SIRT-7 were not associated with IPF.\u003c/p\u003e","manuscriptTitle":"Potential role of SIRT-1 and SIRT-3 as biomarkers for the diagnosis and prognosis of idiopathic pulmonary fibrosis","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-03-14 01:34:26","doi":"10.21203/rs.3.rs-4046708/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Accepted","date":"2024-03-30T12:01:27+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-03-30T11:52:40+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"5256cf53-b3bd-4cf3-8332-2e0f65e06dcb","date":"2024-03-11T18:57:43+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-03-11T18:55:56+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-03-11T18:52:11+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-03-11T07:09:58+00:00","index":"","fulltext":""},{"type":"submitted","content":"Respiratory Research","date":"2024-03-08T15:53:19+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"respiratory-research","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"rere","sideBox":"Learn more about [Respiratory Research](http://respiratory-research.biomedcentral.com/)","snPcode":"12931","submissionUrl":"https://submission.nature.com/new-submission/12931/3","title":"Respiratory Research","twitterHandle":"@RespiratoryBMC","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"1a50cb64-dff1-4bfc-a8c8-21b74826bbdb","owner":[],"postedDate":"March 14th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2024-04-28T00:28:36+00:00","versionOfRecord":{"articleIdentity":"rs-4046708","link":"https://doi.org/10.1186/s12931-024-02796-0","journal":{"identity":"respiratory-research","isVorOnly":false,"title":"Respiratory Research"},"publishedOn":"2024-04-27 00:28:36","publishedOnDateReadable":"April 27th, 2024"},"versionCreatedAt":"2024-03-14 01:34:26","video":"","vorDoi":"10.1186/s12931-024-02796-0","vorDoiUrl":"https://doi.org/10.1186/s12931-024-02796-0","workflowStages":[]},"version":"v1","identity":"rs-4046708","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4046708","identity":"rs-4046708","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}