Construction and screening of cDNA library of differentially expressed genes of endometrium in endometriosis-associated infertility

Objective:To identify the genes differentially expressed in the window of implantation and explore the molecular basis of the development of endometrial receptivity in infertile patients with endometriosis.Methods:A subtracted cDNA library in window of implantation was constructed by suppression subtractive hybridization(SSH) method,then differentially expressed genes of endometrium were identified by gene sequencing and homology comparison.The differential expression of translationally controlled tumor protein(TCTP1) gene,apolipoprotein D(ApoD) gene and erythropoietin(EPO) gene were further confirmed by RT-PCR.Results:After gene sequencing and homology comparison of 20 clones,8 differentially expressed genes were detected in window of implantation.The expression levels of TCTP1,ApoD and EPO in window of implantation were 1.35±0.12,1.24±0.10 and 1.05±0.23,respectively,which were significantly higher than those in control group(1.05±0.23,0.75±0.16,0.68±0.08)(P0.05).Conclusion:SSH is a useful technique to detect differential expression genes of endometrium in window of implantation in endometriosis-associated infertility,which provides a new clue to investigate the molecular basis of the development of endometrial receptivity.