Effect of tempol on post-thaw semen parameters and fertility during chicken semen cryopreservation

ABSTRACT The present study evaluated addition of tempol during chicken semen cryopreservation on post-thaw semen parameters and fertility. Adult PD-1 line semen was cryopreserved using 4% dimethyl sulfoxide (DMSO) in Sasaki diluent (SD). In the semen cryomixture tempol (1 and 5 mM) was added at final concentrations. The semen with additives were filled in 0.5 ml French straws and exposed to liquid nitrogen vapours for 30 min and then stored in liquid nitrogen. The semen straw was thawed at 5°C for 100 sec and evaluated for sperm motility, live, abnormal and acrosome intact sperm. The seminal plasma was evaluated for lipid peroxidation. Fertilizing potential of the cryopreserved sperm was evaluated after insemination in the PD-1 line hens. The post-thaw sperm parameters were significantly (P<0.05) lower in the cryopreserved groups. The lipid peroxidation was significantly (P<0.05) higher in cryopreserved groups. The fertility was significantly (P<0.05) lower in all the cryopreserved groups. In conclusion, addition of tempol to cryopreservation mixture did not improve the post-thaw semen parameters or fertility.