Endometriosis mouse model constructed by anthropo-endometria fragment

objective To provide evidence for endometriosis (EM) pathogenesis by observing the growth of anthropo-menstrual blood endometria in mouse abdominal. Methods During the second and fourth day of the menstrual period, menstrual blood samples were taken from uterine cavity from two EM women and four normal women, centrifugalized at 3 500 r/min for 5 min. The detached endomembrane fragments were then put into the culture fluid (0.9 % NS 4 ml, penililin 100μ, gentamicin 40μ). 1. EM model construction method: 0.2 ml culture fluid (containing 2-4 ednometria fragments ) was teken and then injected into the mouse abdominal cavity, followed by intramuscular peniciline injection (100 ml) in the following two days to prevent infection. 2 Mouse execution method: beginning from the third day since planting, one mouse was executed each day and issues were taken. Results Most of the 15 mouse-models were constructed successfully. There was no significant difference between normal and EM women's endomem-brane in initial planting stage. Multiple planted focuses were found in the abdominal of each mouse. Survival endomembrane cells and glandular organ could be observed on planted focuses in 7 days. Lympho-cytes were however increased gradually. Conclusion The menstrual blood endometria fragment can be successfully planted into mouse abdominal, implying that menstrual blood endometria fragments can further be used as an EM empirical study sample.