m6A function and tumor microenvironment infiltration characteristics in glioma

Background: Most studies related to N 6 -methyladenosine (m 6 A) in glioma have been conducted only at the single-gene level, while the m 6 A modification patterns and correlation between m 6 A and immune cells’ tumor microenvironment infiltration have not been comprehensively reported. Methods Eighty-five clinical samples were obtained from the Gene Expression Omnibus; another 599 clinical samples and 174 transcriptome data were obtained from The Cancer Genome Atlas. Based on data analysis using the R program and principal component analysis, we established an m6Ascore quantitative scoring system and analyzed the modification of 22 m 6 A-related genes with TME infiltration characteristics. Results Three m 6 A modification patterns and the characteristics of immune cell infiltration were identified. Cluster A corresponded to the immune-desert phenotype, cluster B corresponded to the immune-excluded phenotype, and cluster C corresponded to the immune-inflamed phenotype. Cluster B showed the worst long-term prognosis, whereas cluster A had the best long-term prognosis. Anti-CD52/HE5 shows potential as an immune therapy for glioma. Conclusions This research provides a foundation for understanding m 6 A modification patterns in glioma and a potential prognostic biomarker and immune therapy target for treat glioma.