Potential use of Camphor as a new chemoconvulsant model Camphor-induced seizures in rats

Potential use of Camphor as a new chemoconvulsant model Camphor-induced seizures in rats | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Potential use of Camphor as a new chemoconvulsant model Camphor-induced seizures in rats Priscille Fidelis Pacheco Hartcopff, Clarissa Araujo da Paz, Luciana Eiró Quirino, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7895031/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 24 Mar, 2026 Read the published version in Neurotoxicity Research → Version 1 posted 10 You are reading this latest preprint version Abstract Epileptic seizures are a central concern in neurotoxicology, particularly when assessing the mechanisms underlying neuronal hyperexcitability and drug refractoriness. This study investigated the neurobehavioral and electrocorticographic characteristics of seizures induced by camphorated oil in Wistar rats and compared them to those triggered by pentylenetetrazol (PTZ), a classical chemoconvulsant model. Male rats received intraperitoneal administration of camphor (470 mg/kg), and behavioral evolution was monitored to characterize seizure onset and progression. Electrocorticographic (ECoG) recordings from the motor cortex were acquired for 30 minutes to assess cortical oscillatory activity during ictal and interictal periods. Camphor administration elicited six distinct and rapidly evolving behavioral patterns culminating in generalized clonic seizures with loss of postural reflexes. ECoG analyses revealed cyclic high-power ictal discharges interspersed with low-power interictal activity, resembling PTZ-induced seizures but with lower potency. Evaluation of anticonvulsant efficacy demonstrated that camphor-induced seizures were refractory to phenobarbital and phenytoin, whereas diazepam and propofol significantly reduced β- and γ-band power and effectively controlled convulsive activity. These findings suggest that camphor produces a reproducible chemoconvulsant model characterized by prominent cortical excitability and partial pharmacoresistance, supporting its potential utility in neurotoxicological and neuropharmacological studies aimed at understanding seizure mechanisms and testing antiepileptic drug responsiveness. camphor electrocorticography seizure model anticonvulsants Wistar rats Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Introduction Epilepsy is a disorder of the central nervous system characterized by recurrent seizures, which are characterized by an imbalance between excitatory and inhibitory factors, different symptoms depending on the locus of seizure activity and multifactorial causes (Perks et al., 2012 ; Chang et al., 2017; Anwar et al., 2020 ; Falco-Walter, 2020 ; Neligan et al., 2023 ). Among the known causes are electrolyte disorders, systemic diseases affecting the nervous system, hypersensitivity encephalopathy, organ failure, fever and poisoning (Beghi et al., 2010 ; Baluarte, 2017 ; Falco-Walter et al., 2020; Mewasingh et al., 2020 ; El-Dib et al., 2022; Van Bogaert, 2022 ; Rivas-García et al., 2022 ). Treatments for epilepsy include anticonvulsant drugs, resective surgery, and functional surgery, with medication being the main treatment. Antiepileptic drugs primarily target voltage-gated ion channels, such as the update, potassium, and calcium channels, to modulate neuron electrical discharge (Lin & Hsieh, 2021 ). To achieve a better understanding of antiepileptic and epileptogenic drugs, some seizure models are used, such as pentylenetetrazole (PTZ), pilocarpine and kainate (Kebriaeezadeh et al., 2008 ; Klee et al., 2017 ; Samokhina & Samokhin, 2018 ; Landucci et al., 2022 ; Guo et al., 2022 ; Monteiro et al., 2024 ; Monteiro at al., 2024; Gawel et al., 2024 ). From this perspective, scientific research has revealed the existence of other substances with convulsant properties, including some essential oils (EOs), such as eucalyptus and camphor (Mathew et al., 2020 ). Cinnamomum camphora (L.) J. Presl, a large tree belonging to the Lauraceae family, is native to southern China, Taiwan and Japan. This tree is planted to produce camphor (Kang et al., 2019 ; Boroujeni et al., 2022 ; Lee et al., 2022 ). It has been used for centuries around the world as a remedy to treat a variety of symptoms, such as anxiety, inflammation, infection, congestion, pain and irritation (Wang, 2018 ; Lee et al., 2022 ; Fazmiya et al., 2022). The study by Bahr et al. ( 2019 ) points out that camphor has a proconvulsant effect and Mathew et al. (2021) illustrates the similarity of its mechanism of action with that of PTZ. The use of camphor as a seizure-inducing agent was identified in the mid-18th century, as its use was fundamental at that time for the improvement of psychiatric clinical conditions, however, without proper knowledge of its pharmacological properties (Romero-Tapia & Gamboa-Bernal, 2018 ). Many studies with medicinal plants are conducted to investigate possible therapeutic effects, including the components of essential oils, which are natural, volatile and complex phytochemical mixtures that are extracted from aromatic plants as secondary metabolites (Xiao et al., 2021 ). Essential oils are a particular class of natural medicines obtained by distilling plant material to obtain a volatile and hydrophobic extract (Bahr et al., 2019 ). On the other hand, the occurrence of episodes related to side effects unrelated to phytotherapy such as the appearance of convulsions in laboratory animals serve as a good indicator of a chemoconvulsant model as observed with camphor after parenteral application (Gibson, 1989; Ferreira et al., 2020 ; Mathew et al., 2021; Jabbour et al., 2023 ). Thus, the aim of this study was to evaluate the potential of camphor as a chemoconvulsant model and to characterize the behaviour of the animals during the evolution of the seizure, as well as to assess refractoriness to treatment with anticonvulsant drugs. The choice of wistar rats is important since, in studies of neurotoxicology and epileptogenesis, they offer a stable neurological response comparable to human models, which facilitates the extrapolation of findings and includes new models for the study of the underlying mechanisms that are revealed in seizures. Materials and Methods Animals For the study, heterogeneous male Wistar rats (n = 63) aged between 9 and 10 weeks and weighing between 180g and 210g were used. They came from the Central Animal Facility of the Federal University of Pará ICB - UFPA and were allocated to the animal facility of the Laboratory of Pharmacology and Toxicology of Natural Products - LFTPN -ICB-UFPA. The animals were acclimatized to laboratory conditions 5 days before the experimental manipulation. They were placed in boxes measuring 50cm x 60cm x 20cm (height x width x depth) with wood shavings, filtered water and commercial feed with 32% protein from Purina. They were kept at an ambient temperature of 25–26°C, in 12- hour light/12-hour dark cycles. All the experiments were carried out from 8:00 am to 11:00 am. The entire experimental protocol was by Animal Use Ethics Committee of the Federal University of Pará (CEUA/UFPA) No. 8381060818 (ID 000975). Chemical products The drugs were purchased from the following sources: ketamine - König (Santana de Parnaíba, SP, Brazil); xylazine - Vallée (Montes Claros, MG, Brazil). Anticonvulsant drugs phenobarbital (Aventis-Pharma, Ribeirão Preto, SP, Brazil); phenytoin, diazepam (União Química, Embu-Guaçu, SP, Brazil), Injectable sodium valproate (Depacon) (Abbott – Laboratories of Brazil Ltda, São Paulo, Brazil), Propofol (Union Chemistry – Guarulhos- SP- Brazil), Local anesthetic Lidocaine hydrochloride 2% from the Cristália laboratory (Travessa Sinamomo 70, Bairro Arvoredo, Concórdia, SC, Brazil) Ketoprofen Injectable 100 mg Eurofarma Laboratory S.A (Rod. Pres. Castello Branco, 3565 - Itapevi - SP), the pro-convulsant drug Pentylenetetrazole (PTZ) (SIGMA) and camphorated oil company UCBVET 2.35 g/10 mL. Experimental design Experiment 1: Behavioral assessment . The animals were evaluated according to the evolution of the seizures. The dose of camphor was calculated according to the time taken for the seizures to progress to generalized clonic seizures with loss of the posture reflex between 5 and 10 minutes. Thus, the dose used was 470 mg/kg via i.p. Latency times were assessed for the following behaviors: 1. immobility; 2. head and neck tremor; 3. fanned tail; 4. forelimb clonus; 5. generalized clonic seizure without loss of posture reflex and 6. generalized clonic seizure with loss of posture reflex. Generalized seizure with loss of posture reflex. Observation lasted 10 minutes. Experiment 2: Electrocorticographic recording (EcoG) Surgery for electrode implantation. The animals were anaesthetized by intraperitoneal injection of a combination of 10% ketamine hydrochloride at a dose of 100 mg / kg i.p. and 2% xylazine hydrochloride at a dose of 5 mg / kg i.p. The degree of anaesthesia depth was assessed using the pain reflex after the interdigital reflex test. In cases where the reflex was still present, anesthetic supplementation was carried out with 1/4 of the dose initially applied. During anesthesia, the animals were positioned in a stereotactic apparatus for trichotomy of the head and application of the local anesthetic Lidocaine to the incised skin. After asepsis with alcoholic iodopolivinylpyrolidone (PVPI) O, surgery was performed to expose the skull. Two bilateral holes were drilled into the rat's skull with a dental drill. Activated silver electrodes (0.5 mm diameter tip exposure) were placed in the dura mater above the frontal cortex at breech coordinates − 0.96 mm and ± 1.0 mm lateral (Hamoy et al., 2018 ) in the motor cortex region. A reinforcement screw was fixed to the skull, and the electrodes were fixed with dental acrylic cement (self-curing acrylic). Electrocorticographic recording Five days after the electrodes were implanted, the groups were administered the drugs according to the following design: a) Control group (received the equivalent volume of 0.9% saline solution); b) group treated with pentylenetetrazole 60 mg/kg i.p. ; c) Group treated with camphorated oil 470 mg/kg i.p. The recordings lasted 30 minutes and nine animals were used for each experiment. The recordings were evaluated in terms of their power in oscillations up to 40 Hz, including the following ranges of brain oscillations: Delta (1–4 Hz), Theta (4–8 Hz), Alpha (8–12 Hz), Beta (12–28 Hz) and Gamma (28–40 Hz). Experiment 3 - Performance of anticonvulsants Anticonvulsant drugs were tested after the application of camphor 470 mg/kg i.p., the clinical trial was carried out with three different anticonvulsants: Phenobarbital (10 mg/kg i.p.), Phenytoin (10 mg/kg i.p.), Diazepam (10 mg/kg i.p.), Sodium valproate (10 mg/kg i.p.), Propofol (10 mg/kg i.v.) lateral tail vein. To assess the control of seizures by anticonvulsants, the animals were treated with camphor followed by the administration of anticonvulsants; the recordings lasted 15 minutes. N = 9 was used for each group. Analysis of the recordings The recordings were obtained using a differential amplifier with high AC input impedance (Grass Technologies, Model P511) adjusted with 0.3 HZ and 3 KHz filtering, monitored with an oscilloscope (Protek, Model 6510) and continuously digitized at a rate of 1 KHz by a computer equipped with a data acquisition card (National Instruments, Austin, TX). The seizures were characterized using the Python programming language (version 2.7) and the Signal ® 3.0 program. For signal acquisition. Analyses were carried out at a frequency of up to 40 Hz and divided into bands according to Hamoy et al. ( 2018 ) into Beta (1–4 Hz), Theta (4–8 Hz), Alpha (8–12), Beta (12–28) and Gamma (28–40 Hz), to interpret the dynamics during seizure development. Euthanasia After carrying out the experiments, the animals were euthanized with a lethal injection of 300mg/kg i.p. of ketamine associated with 30 mg/kg i.p. of xylazine hydrochloride and 10 mg/kg i.p. of diazepam; this procedure was necessary to avoid suffering the animals. Statistical analysis The results were submitted to descriptive statistics, such as mean and standard deviation. One-way analysis of variance (ANOVA) was used, followed by a Tukey test. A significance index of *p < 0.05 was adopted. **p < 0.01, ***p < 0.001. Results Description of the characteristic behavior related to Camphor-induced convulsion. After the administration of camphorated oil, the animals showed a rapid evolution of the seizure, displaying six behaviors. The change in behavior begins with immobility (akinesia) with an average latency of 78.89 ± 18.73 s, followed by head and neck tremors (135.9 ± 17.03 s) and tail stiffening (178 ± 17.59 s). The next behaviour involves the somatic system more intensely, revealing itself as clonias of the forelimbs (219.6 ± 37.37 s), the crisis evolving into a generalized clonic convulsion without loss of the posture reflex (281 ± 38.70 s) and with a worsening of the crises causing generalized clonic convulsions with loss of the posture reflex (384.9 ± 74.41 s). Each component of the behaviors recorded above is part of the subsequent behaviors. Insert Fig. 1 about here There are reports in the literature of Cinnamomum camphora inducing or provoking tonic-clonic seizures in patients with and without a history of seizures. Camphor induced generalized tonic-clonic seizures in 95% of the cases in a study with human patients with no previous history of seizures (p = 22) and caused generalized tonic- clonic seizures in 27.3% and localized or bilateral seizures in 15% of the other group of patients in the study, with a history of epilepsy or drug seizures (p = 33) (Mathew et al, 2021). Camphor-induced seizures have similar characteristics to the PTZ-induced chemoconvulsant model. The readings of the ECoG recordings for the control group showed low amplitudes (0.05 mV), which keeps the recording regular, so the spectrogram shows higher levels of energy distributed at frequencies below 10 Hz (Fig. 2 A and B). The amplification of the recording shows morphographic components related to the activity of the rat's motor cortex, characterized by low amplitude with a predominance of low-frequency brain oscillations (Fig. 2 C). Insert Fig. 2 about here The PTZ-treated group showed changes in the ECoG tracing with cyclic activity reaching amplitudes above 0.5 mV during ictal status, which are characteristic of seizures, and interictal status, which was characterized by a decrease in the amplitude of the recording to the range of 0.05 mV. After the application of PTZ, the latency for the onset of seizures was 48 ± 24s. The frequency spectrogram shows an increase in power in oscillations distributed up to 40 Hz (Fig. 3 A). The group treated with camphor oil showed intercalated electrocorticographic tracings between ictal and interictal status, characterized by neuronal recruitment with an increase in field potential during ictal status, which showed an increase in the power of the recordings, and interictal status with a decrease in power; the latency period for occurrence was 78.89 ± 18.73 s. The spectrogram shows a greater distribution of energy during ictal status (Fig. 3 B). Insert Fig. 3 about here The spectral power distribution revealed greater amplitude in all brain waves in the camphor and PTZ groups compared to the control group (Fig. 4 A). Power in the delta, theta and alpha frequencies was greater in the PTZ group, while gamma oscillations were more powerful in the camphor group. In this way, the average power in linear oscillations up to 40 Hz for the control group was 0.366 ± 0.086 mV² / Hz x 10-³ was lower than the groups treated with PTZ 9.11 ± 1.78 mV² / Hz x 10-³ and camphorated oil 6.46 ± 1.77 mV² / Hz x 10-³ (Fig. 4 B). Mean analyses of the delta, theta, alpha, beta and gamma brain frequency bands showed an increase compared to the control group. Thus, PTZ and camphorated oil were able to induce disturbances in all brain waves, but the animals treated with camphorated oil showed greater power in gamma oscillations compared to the PTZ group (Fig. 4 A). For delta oscillations, the control group had a lower mean power (0.109 ± 0.022 mV² / Hz x 10-³) than the other groups PTZ (0.814 ± 0.171 mV² / Hz x 10-³) and camphorated oil (0.271 ± 0.0620 mV² / Hz x 10-³). The group treated with PTZ had a higher mean than the group treated with camphorated oil (Fig. 4 C). The mean power in theta oscillations for the control group (0.723 ± 0.017 mV² / Hz x 10-³) was lower than the PTZ (2.141 ± 0.263 mV² / Hz x 10-³) and camphorated oil (0.321 ± 0.048 mV² / Hz x 10-³) groups. The group treated with PTZ was superior to the group treated with camphorated oil (Fig. 4 D). In alpha oscillations, the control group had a mean of 0.033 ± 0.007 mV²/Hz x 10- ³, which was lower than the groups treated with PTZ (2.35 ± 0.51 mV²/Hz x 10-³) and camphorated oil (0.60 ± 0.153 mV²/Hz x 10-³). The group treated with camphorated oil was inferior to the group treated with PTZ (Fig. 4 E). For beta oscillations, the control group had a mean of 0.013 ± 0.006 mV² / Hz x 10-³, which was lower than the treated groups. The PTZ group had a mean power of 2.13 ± 0.354 mV²/Hz x 10-³ and was similar to the group treated with camphorated oil (p = 0.1928) (Fig. 4 F). For gamma oscillations, the control group (0.0059 ± 0.0011 mV²/Hz x 10-³) was lower than the other treated groups. The camphor oil-treated group (0.730 ± 0.141 mV²/Hz x 10-³) was superior to the PTZ-treated group (0.471 ± 0.141 mV²/Hz x 10-³) (Fig. 4 G). Insert Fig. 4 about here The spectral distribution graph of power at frequencies up to 40 Hz in the recordings of camphorated oil-induced seizures was divided into two distinct periods during the recording that occur in a cyclical manner, in the ictal status period a higher level of power was observed and in the interictal status period a lower level of power was observed in the oscillations (Fig. 5 A). The average power for the control group (0.366 ± 0.086 mV²/Hz x 10-³) was similar to the interictal status group (p = 0.075). However, all groups had lower mean power during ictal status (10.25 ± 1.661 mV²/Hz x 10-³) (Fig. 5 B). For delta oscillations (1–4 Hz), the control group (0.109 ± 0.022 mV²/Hz x 10-³) had a mean power similar to the interictal period (p = 0.092), but was lower than the camphor oil (0.271 ± 0.062 mV²/Hz x 10-³) and ictal status (0.351 ± 0.081 mV²/Hz x 10-³) groups. The ictal status group was superior to all groups (Fig. 5 C). The theta oscillations in the status ictal period (0.433 ± 0.114 mV²/Hz x 10-³) were higher than in the other groups. The interictal period had a higher mean (0.197 ± 0.100 mV²/Hz x 10-³) than the control group (0.072 ± 0.017 mV²/Hz x 10-³) (Fig. 5 D). For alpha oscillations, the mean power during ictal status (0.872 ± 0.308 mV²/Hz x 10-³) was higher than in the other groups. The average power for interictal status (0.225 ± 0.063 mV²/Hz x 10-³) was similar to the control group (p = 0.1129) (Fig. 5 E). The average power of beta oscillations during the ictal status period (3.18 ± 0.364 mV²/Hz x 10-³) was higher than the other groups. The control group (0.013 ± 0.0065 mV²/Hz x 10-³) was lower than the interictal status period (0.762 ± 0.212 mV²/Hz x 10³) (Fig. 5 F). For gamma oscillations, the control group (0.005 ± 0.001 mV²/Hz x 10-³) was lower than the other groups. The average power for the ictal status period (1.20 ± 0.25 mV²/Hz x 10-³) was higher than the other groups. The camphorated oil group (0.73 ± 0.141 mV²/Hz x 10-³) was superior to the interictal status group (Fig. 5 G). Insert Fig. 5 about here Camphor had a greater preponderance in the power of beta and gamma oscillations and was refractory to treatment with phenobarbital and phenytoin. To assess seizure control observed after camphorated oil treatment, the animals were treated with anticonvulsants, and then ECoGs were recorded, and their power in beta (12–28 Hz) and gamma (28–40 Hz) oscillations was assessed. Seizure control after phenobarbital use was observed 10 minutes after recording. During the first 10 minutes, periods of ictal and interictal states could still be identified (Fig. 6 A). Phenytoin and sodium valproate were ineffective in controlling seizures, with the ictal and interictal states maintained throughout the recording, demonstrating that the chemoconvulsant model induced by camphorated oil was refractory to the treatments (Fig. 6 B and D). Seizure control was effectively achieved with diazepam and propofol, which indicated better protection for the animals (Fig. 6 C and E), with propofol being more effective. Recording patterns obtained with the use of anticonvulsants are shown for beta and gamma oscillations (Figs. 6 F and G). The mean beta oscillation in the control group was 0.013 ± 0.006 mV²/Hz x 10 − 3 , similar to the group treated with Diazepam (p = 0.959) and propofol (p = 0.999), but was lower than the other groups. The group treated with phenytoin (1.41 ± 0.29 mV²/Hz x 10 − 3 ) was superior to the other groups treated with anticonvulsants. The group treated with phenobarbital (0.659 ± 0.178 mV²/Hz x 10 − 3 ) and lower than the camphorated oil group, treated with phenytoin and sodium valproate. The diazepam-treated group (0.0942 ± 0.0193 mV²/Hz x 10 − 3 was similar to the propofol-treated group (p = 0.999) (Fig. 6 F). For gamma oscillations, the control group (0.005 ± 0.0011 mV²/Hz x 10 − 3 ) was similar to the diazepam group (p = 0.998) and the propofol-treated group (p = 999). The phenobarbital group (0.523 ± 0.128 mV²/Hz x 10 − 3 ) was similar to the phenytoin group (p = 0.923) and the sodium valproate-treated group (p = 0.169), but was inferior to the camphor group. The phenytoin-treated group (0.471 ± 0.094 mV²/Hz x 10 − 3 ) was similar to the group treated with sodium valproate (p = 0.787). The group treated with Diazepam (0.0196 ± 0.004 mV²/Hz x 10 − 3 ) was similar to the group treated with propofol (p = 0.999) (Fig. 6 G). Insert Fig. 6 about here Discussion The behavioral data showed that camphor presented rapid evolution of convulsive behavior, six behaviors were identified culminating in generalized clonic convulsion with loss of the posture reflex (384.9 ± 74.41 s) after application. The behaviours presented the following components: akinesia, head and neck tremor, tail stiffening, forelimb clonias, generalized clonic seizures without loss of posture reflex and generalized clonic seizures with loss of posture reflex, with the subsequent behaviours presenting components of the previous behaviours. These data indicate possible points of intersection between the chemoconvulsant model promoted by the administration of camphor and models traditionally studied with other chemical agents such as pentylenetetrazole and kainic acid, which represent suitable models for the study of refractory epilepsy to screen the activity of anticonvulsant drugs and which help to clarify the mechanisms involved in convulsive and epileptic processes (Taylor et al., 1981; Lüttjohann et al., 2009 ; Van Erum et al.,2019; Ishikawa et al., 2020 ; de Almeida et al., 2020 ; Sing et al., 2021; Alharbi, 2021 ; Dos Santos et al., 2021 ; Torun, Kilinc & Kilinc, 2022). Although some differences can be noted, such as the latency for the onset of seizure behavior and the dose, which shows that PTZ is more potent in triggering seizures when compared to camphor. A study by Hamoy et al. ( 2018 ) demonstrated the activity of the cequisterpene cunaniol obtained from wild Clibadium as a powerful GABA antagonist chemoconvulsant with greater potency than PTZ, indicating that natural substances can be used as new convulsive models. In the electrocorticogram, the morphographic elements showed a similarity to the recrudescence of activity that increases the field potential recorded, characterized as a salvo of potentials that results in the ictal period, which is characterized by high power in the recording, followed by a decrease in power corresponding to the post-ictal period; these morphographic characteristics can be observed in both camphor-induced and PTZ- induced seizures. This characteristic was also observed for cunaniol and caffeine-induced seizures (Hamoy et al. 2018 ; Eiró-Quirino et al., 2024 ). In general, there is an increase in the power of brain oscillations up to 40 Hz during camphor- and PTZ-induced seizures when compared to the control group. The breakdown of brain oscillations indicates that Delta (1–4 Hz), Theta (4-8Hz) and Alpha (8–12 Hz) powers were higher in the PTZ-treated group, but beta (12–28 Hz) oscillations were similar, and gamma power was higher for camphor, indicating a preponderance of forces in seizures induced at frequencies of (28-40Hz). Leitão et al., ( 2022 ) studying seizures provoked by thiocolchicoside in rats observed an increase in the powers of cerebral oscillations in the motor cortex during the ictal period. These effects were also observed for seizures induced by nicotine and pilocarpine (Da Silva et al., 2022 ). Regarding the characteristics of camphor-induced seizures, ictal and inter-ictal periods were observed. During the ictal period, the average power of the brain oscillations increased towards Beta and Gamma oscillations, with a characteristic higher frequency between (12–40 Hz). According to Naggar et al. ( 2020 ) field potential oscillations reflect repetitive firing of neuronal synaptic activity in certain areas of the brain that can be distinguished as slow (alpha, delta and theta), fast (beta and gamma). In this way, during the ictal period caused by camphor, fast powers predominate. These ictal characteristics have also been observed in the outbreak-suppression that occurs in children with West syndrome (Nariai et al., 2017 ). When using anticonvulsants such as phenobarbital, phenytoin, diazepam, sodium valproate and propofol to control seizures and observing after treatment with camphor in the reduction of average power in beta and gamma oscillations. The best efficacy was observed with the application of diazepam which positively modulates the response to GABA, as well as binding to the allosteric site of the GABAA receptor which results in an increase in the frequency of opening of the Cl - channel when GABA is present, hyperpolarizing the cell and making it difficult to fire the action potential., reducing the average power in beta and gamma. Zhou et al. ( 2021 ) demonstrated through a randomized clinical trial, with seizure models using lithium and pilocarpine in adult rats, that diazepam, when administered within 10 minutes of seizure onset, has better results through global suppression of power. Similarly, Rojas et al. ( 2018 ) demonstrated seizure interruption, but power in the ~ 20–70 Hz bands (beta and gamma) returned hours later in rats with a seizure model using diisopropylfluorophosphate. Phenobarbital was more effective at controlling average beta power when compared to the phenytoin group, this can be justified by the affinity with a specific site of GABAA receptors, causing a prolongation of the opening time of these channels, resulting in a greater influx of Cl - , as well as a reduction in the action of AMPA receptors and the blocking of L and N type Ca 2+ channels. This corroborates other studies involving the control of seizures induced by pro-convulsant substances where treatment with diazepam was very effective (Mandhane et al., 2007 ; Akula et al., 2009 ; Mikutra-Cencora & Teitelbaum, 2025 ). As was Propofol because it also binds to the allosteric site of the GABAA receptor, it has the particularity of a GABA-mimetic effect at higher concentrations, as well as the inhibition of NMDA receptors, breaking the cycle of hyperexcitability. Although phenytoin delays the reactivation of Na + channels in a use-dependent manner by inhibiting the most active neurons during seizures, in our study it did not show adequate efficacy in the ictal and interictal states, which may be explained by refractory blockades and alternative triggering pathways, such as Ca channels and glutamate receptors. Furthermore, changes in pH and extracellular ions during the ictal state can alter the kinetics of Na + channels, reducing the affinity of phenytoin, corroborating the study by West et al. ( 2022 ) who observed that phenytoin was not effective in suppressing resistance seizures, as well as in the interictal period. As well as sodium valproate, which has a similar action in the context of blocking voltage-dependent Na + channels, together with the stimulation of glutamate decarboxylase and blocking of T-type Ca 2+ channels, In this context, Richardson, Petrou and Bryson ( 2024 ) further highlighted that it is possible that it can increase the synaptic release of GABA, as well as modulate metabolic pathways, through the inhibition of alpha-ketoglutarate, increasing activity through the diversion of GABA. In the present study, we describe the behavioral and electrophysiological characteristics during seizures provoked by the application of Camphor, using epidural ECoG recordings in the motor cortex of rats. Treatment with camphor resulted in changes in the graph-elements of the recording, which exhibited characteristics of convulsant activity, confirming that camphor is a potent convulsant agent in the rat brain. The induced seizures show a cyclical pattern with cellular recruitment triggering a salvo of potentials in the ictal and post-ictal periods, with a preponderance of beta and gamma oscillations during the ictal period. Control of camphor-induced seizures was better achieved with diazepam and propofol. In this way, the use of camphor makes it a suitable model for studies that address the mechanism involved in neurotransmission to assess the refractoriness of anticonvulsant drugs. Declarations Author Contributions Priscille Fidelis Pacheco Hartcopff, Moisés Hamoy contributed to the conception, design of the study and review of the final manuscript. Clarissa Araujo da Paz, Luciana Eiró Quirino, Thaysa de Sousa Reis, Daniella Bastos de Araujo, Axell Lins contributed to the experimental design and original text. Rafael dos Reis de Souza, Caio Gabriel da Silva Motta, Mateus Felipe Ferreira Araújo, Marcelo Victor dos Santos Brito, Murilo Farias dos Santos, Alisson Bruno Leite Lima, Moisés Hamoy contributed to the methodology implementation and data analysis. Acknowledgements We thank the Coordination for the Improvement of Higher Education Personnel, the Federal University of Pará and the Institute of Biological Sciences Data availability The datasets generated are available from the corresponding author on reasonable request. Funding The authors have nothing to report. Ethics statement All procedures were approved by Animal Use Ethics Committee of the Federal University of Pará (CEUA/UFPA) Nº 8381060818 (ID 000975). Consent to participate/Consent for publication Not applicable Conflict of interest statement The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. 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06:44:30","extension":"html","order_by":36,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":128414,"visible":true,"origin":"","legend":"","description":"","filename":"earlyproof.html","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/8a03faa5e74ed534c7f4eb53.html"},{"id":95525536,"identity":"dc4e88ac-a460-440a-b4ea-e1748cc5f344","added_by":"auto","created_at":"2025-11-10 10:05:14","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":146776,"visible":true,"origin":"","legend":"\u003cp\u003eDescription of camphor-induced seizure-related behavior according to the latency for observing the behavior.\u003c/p\u003e","description":"","filename":"Figure1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/81f4d3a1c988a1b23838e2d1.jpg"},{"id":95525653,"identity":"4c342b95-fc9a-4799-b429-a459cbc4c1a6","added_by":"auto","created_at":"2025-11-10 10:05:31","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":275290,"visible":true,"origin":"","legend":"\u003cp\u003eElectrocorticographic recordings of the motor cortex of the control group (A), spectrogram of energy distribution up to 40 Hz (B), amplification of the recordings showing a duration of one second (899 to 900s) (C), recordings lasting 20 minutes.\u003c/p\u003e","description":"","filename":"FIgure2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/16f3f18a653184a3261b586c.jpg"},{"id":95356777,"identity":"46671fd4-9d14-47c3-ba0c-b16539971b8c","added_by":"auto","created_at":"2025-11-07 06:44:29","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":465723,"visible":true,"origin":"","legend":"\u003cp\u003eDemonstrations of electrocorticographic (ECoG) recordings lasting 30 minutes. ECoG tracing of the group treated with pentylenetetrazole (PTZ) (left), amplification showing graphic components of ictal status (top center), amplification showing graphical elements of interictal status (bottom center) and spectrogram of energy distribution up to 40 Hz (right) (A). Electrocorticographic (ECoG) recording lasting 30 minutes of the camphorated oil-treated group (left), amplification of ictal status (top center), amplification of interictal status (bottom center) and power distribution spectrogram at frequencies up to 40 Hz (B).\u003c/p\u003e","description":"","filename":"FIgure3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/220765fa44f213995ac0f682.jpg"},{"id":95524292,"identity":"b9f931a2-e573-40e2-a892-7411eb17694a","added_by":"auto","created_at":"2025-11-10 10:02:36","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":303207,"visible":true,"origin":"","legend":"\u003cp\u003eSpectral distribution of power at frequencies up to 40 Hz for the control, PTZ and camphor recordings (A); Graph representing the average linear power up to 40 Hz for the groups (B); Graphs of power distribution in brain oscillations up to 40 Hz: Delta ( 1-4 Hz) (C), Theta (4 - 8Hz) (D), Alpha (8-12 Hz) (E), Beta ( 12-28 Hz) (F) and Gamma (28- 40 Hz) (G). After ANOVA followed by Tukey, * P\u0026lt;0.05 ** P\u0026lt;0.01 ***P\u0026lt;0.001, n=9).\u003c/p\u003e","description":"","filename":"Figure4.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/139aea36f3ddb841f3cfdc79.jpg"},{"id":95356783,"identity":"8b0bb145-9a41-40fc-8eec-6e199b6aed4c","added_by":"auto","created_at":"2025-11-07 06:44:29","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":258121,"visible":true,"origin":"","legend":"\u003cp\u003eSpectral power distribution graph at frequencies up to 40 Hz in ECoG recordings during the seizure in the camphor oil-treated group and in the ictal status and interictal status periods (A); Linear power averages during ictal status and interictal status (B); Linear power average between groups with frequency in delta oscillations (1-4 Hz) (C); Average power distribution in theta frequency (4-8 Hz) (D); Graph of linear power distribution in the recordings between the groups in alpha oscillations (8-12 Hz) (E); Average power distribution in beta frequency (12-28 Hz) (F); Graph of linear power distribution between the groups in gamma frequency (28-40 Hz) (G).(After ANOVA followed by Tukey, * P\u0026lt;0.05 ** P\u0026lt;0.01 ***P\u0026lt;0.001, n=9).\u003c/p\u003e","description":"","filename":"Figure5AB.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/769673914b65339192a6b403.jpg"},{"id":95525837,"identity":"27dedd6a-7162-43e8-95b6-6354b33d1db0","added_by":"auto","created_at":"2025-11-10 10:05:43","extension":"jpg","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":499016,"visible":true,"origin":"","legend":"\u003cp\u003eDemonstrative electrocorticographic (ECoG) recordings lasting 15 minutes from the application of camphorated oil followed by the application of the following anticonvulsants: Phenobarbital (10mg/kg i.p.) (A); Phenytoin (10 mg/kg i.p.) B); Diazepam (10mg/kg i.p.) (C) Sodium valproate (10 mg/kg i.p.) (D), Propofol (10mg/kg i.v.) (E)and graph with power averages in beta (12-28 Hz) (F) and Gamma (28-40Hz) (G) frequencies. (After ANOVA followed by Tukey, * P\u0026lt;0.05 ** P\u0026lt;0.01 ***P\u0026lt;0.001, n=9).\u003c/p\u003e","description":"","filename":"Figure6ABC.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/c6cb0f5a1036d763aa75be07.jpg"},{"id":105755635,"identity":"3d004156-a819-40be-90bb-c86f28181c82","added_by":"auto","created_at":"2026-03-30 16:28:44","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2886890,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/b0bf48f8-69ab-44d5-8aee-aee5c0ffb6fd.pdf"},{"id":95356779,"identity":"9a3fe320-3981-4147-b46d-d4e5904ed02e","added_by":"auto","created_at":"2025-11-07 06:44:29","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":30859,"visible":true,"origin":"","legend":"","description":"","filename":"Highlights.docx","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/1b7186aa9ef1a859ccfdeae0.docx"},{"id":95356781,"identity":"4b0b5a78-f522-44af-a234-938e587b6430","added_by":"auto","created_at":"2025-11-07 06:44:29","extension":"png","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":1736239,"visible":true,"origin":"","legend":"","description":"","filename":"GraphicalAbstract.png","url":"https://assets-eu.researchsquare.com/files/rs-7895031/v1/69a96bb877472da88cb17675.png"}],"financialInterests":"No competing interests reported.","formattedTitle":"Potential use of Camphor as a new chemoconvulsant model Camphor-induced seizures in rats","fulltext":[{"header":"Introduction","content":"\u003cp\u003eEpilepsy is a disorder of the central nervous system characterized by recurrent seizures, which are characterized by an imbalance between excitatory and inhibitory factors, different symptoms depending on the locus of seizure activity and multifactorial causes (Perks et al., \u003cspan citationid=\"CR39\" class=\"CitationRef\"\u003e2012\u003c/span\u003e; Chang et al., 2017; Anwar et al., \u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e2020\u003c/span\u003e; Falco-Walter, \u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e2020\u003c/span\u003e; Neligan et al., \u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e2023\u003c/span\u003e). Among the known causes are electrolyte disorders, systemic diseases affecting the nervous system, hypersensitivity encephalopathy, organ failure, fever and poisoning (Beghi et al., \u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e2010\u003c/span\u003e; Baluarte, \u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e2017\u003c/span\u003e; Falco-Walter et al., 2020; Mewasingh et al., \u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e2020\u003c/span\u003e; El-Dib et al., 2022; Van Bogaert, \u003cspan citationid=\"CR47\" class=\"CitationRef\"\u003e2022\u003c/span\u003e; Rivas-Garc\u0026iacute;a et al., \u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e2022\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eTreatments for epilepsy include anticonvulsant drugs, resective surgery, and functional surgery, with medication being the main treatment. Antiepileptic drugs primarily target voltage-gated ion channels, such as the update, potassium, and calcium channels, to modulate neuron electrical discharge (Lin \u0026amp; Hsieh, \u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e2021\u003c/span\u003e). To achieve a better understanding of antiepileptic and epileptogenic drugs, some seizure models are used, such as pentylenetetrazole (PTZ), pilocarpine and kainate (Kebriaeezadeh et al., \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e2008\u003c/span\u003e; Klee et al., \u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e2017\u003c/span\u003e; Samokhina \u0026amp; Samokhin, \u003cspan citationid=\"CR44\" class=\"CitationRef\"\u003e2018\u003c/span\u003e; Landucci et al., \u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e2022\u003c/span\u003e; Guo et al., \u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e2022\u003c/span\u003e; Monteiro et al., \u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e2024\u003c/span\u003e; Monteiro at al., 2024; Gawel et al., \u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). From this perspective, scientific research has revealed the existence of other substances with convulsant properties, including some essential oils (EOs), such as eucalyptus and camphor (Mathew et al., \u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e2020\u003c/span\u003e).\u003c/p\u003e\u003cp\u003e\u003cem\u003eCinnamomum camphora\u003c/em\u003e (L.) J. Presl, a large tree belonging to the Lauraceae family, is native to southern China, Taiwan and Japan. This tree is planted to produce camphor (Kang et al., \u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e2019\u003c/span\u003e; Boroujeni et al., \u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e2022\u003c/span\u003e; Lee et al., \u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e2022\u003c/span\u003e). It has been used for centuries around the world as a remedy to treat a variety of symptoms, such as anxiety, inflammation, infection, congestion, pain and irritation (Wang, \u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2018\u003c/span\u003e; Lee et al., \u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e2022\u003c/span\u003e; Fazmiya et al., 2022). The study by Bahr et al. (\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e2019\u003c/span\u003e) points out that camphor has a proconvulsant effect and Mathew et al. (2021) illustrates the similarity of its mechanism of action with that of PTZ. The use of camphor as a seizure-inducing agent was identified in the mid-18th century, as its use was fundamental at that time for the improvement of psychiatric clinical conditions, however, without proper knowledge of its pharmacological properties (Romero-Tapia \u0026amp; Gamboa-Bernal, \u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e2018\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eMany studies with medicinal plants are conducted to investigate possible therapeutic effects, including the components of essential oils, which are natural, volatile and complex phytochemical mixtures that are extracted from aromatic plants as secondary metabolites (Xiao et al., \u003cspan citationid=\"CR51\" class=\"CitationRef\"\u003e2021\u003c/span\u003e). Essential oils are a particular class of natural medicines obtained by distilling plant material to obtain a volatile and hydrophobic extract (Bahr et al., \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e2019\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eOn the other hand, the occurrence of episodes related to side effects unrelated to phytotherapy such as the appearance of convulsions in laboratory animals serve as a good indicator of a chemoconvulsant model as observed with camphor after parenteral application (Gibson, 1989; Ferreira et al., \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e2020\u003c/span\u003e; Mathew et al., 2021; Jabbour et al., \u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e2023\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eThus, the aim of this study was to evaluate the potential of camphor as a chemoconvulsant model and to characterize the behaviour of the animals during the evolution of the seizure, as well as to assess refractoriness to treatment with anticonvulsant drugs. The choice of wistar rats is important since, in studies of neurotoxicology and epileptogenesis, they offer a stable neurological response comparable to human models, which facilitates the extrapolation of findings and includes new models for the study of the underlying mechanisms that are revealed in seizures.\u003c/p\u003e"},{"header":"Materials and Methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e\u003ch2\u003eAnimals\u003c/h2\u003e\u003cp\u003eFor the study, heterogeneous male Wistar rats (n\u0026thinsp;=\u0026thinsp;63) aged between 9 and 10 weeks and weighing between 180g and 210g were used. They came from the Central Animal Facility of the Federal University of Par\u0026aacute; ICB - UFPA and were allocated to the animal facility of the Laboratory of Pharmacology and Toxicology of Natural Products - LFTPN -ICB-UFPA.\u003c/p\u003e\u003cp\u003eThe animals were acclimatized to laboratory conditions 5 days before the experimental manipulation. They were placed in boxes measuring 50cm x 60cm x 20cm (height x width x depth) with wood shavings, filtered water and commercial feed with 32% protein from Purina. They were kept at an ambient temperature of 25\u0026ndash;26\u0026deg;C, in 12- hour light/12-hour dark cycles. All the experiments were carried out from 8:00 am to 11:00 am. The entire experimental protocol was by Animal Use Ethics Committee of the Federal University of Par\u0026aacute; (CEUA/UFPA) No. 8381060818 (ID 000975).\u003c/p\u003e\u003c/div\u003e\n\u003ch3\u003eChemical products\u003c/h3\u003e\n\u003cp\u003eThe drugs were purchased from the following sources: ketamine - K\u0026ouml;nig (Santana de Parna\u0026iacute;ba, SP, Brazil); xylazine - Vall\u0026eacute;e (Montes Claros, MG, Brazil). Anticonvulsant drugs phenobarbital (Aventis-Pharma, Ribeir\u0026atilde;o Preto, SP, Brazil); phenytoin, diazepam (Uni\u0026atilde;o Qu\u0026iacute;mica, Embu-Gua\u0026ccedil;u, SP, Brazil), Injectable sodium valproate (Depacon) (Abbott \u0026ndash; Laboratories of Brazil Ltda, S\u0026atilde;o Paulo, Brazil), Propofol (Union Chemistry \u0026ndash; Guarulhos- SP- Brazil), Local anesthetic Lidocaine hydrochloride 2% from the Crist\u0026aacute;lia laboratory (Travessa Sinamomo 70, Bairro Arvoredo, Conc\u0026oacute;rdia, SC, Brazil) Ketoprofen Injectable 100 mg Eurofarma Laboratory\u003c/p\u003e\u003cp\u003eS.A (Rod. Pres. Castello Branco, 3565 - Itapevi - SP), the pro-convulsant drug Pentylenetetrazole (PTZ) (SIGMA) and camphorated oil company UCBVET 2.35 g/10 mL.\u003c/p\u003e\n\u003ch3\u003eExperimental design\u003c/h3\u003e\n\u003cp\u003e\u003cb\u003eExperiment 1: Behavioral assessment\u003c/b\u003e.\u003c/p\u003e\u003cp\u003eThe animals were evaluated according to the evolution of the seizures. The dose of camphor was calculated according to the time taken for the seizures to progress to generalized clonic seizures with loss of the posture reflex between 5 and 10 minutes. Thus, the dose used was 470 mg/kg via i.p.\u003c/p\u003e\u003cp\u003eLatency times were assessed for the following behaviors: 1. immobility; 2. head and neck tremor; 3. fanned tail; 4. forelimb clonus; 5. generalized clonic seizure without loss of posture reflex and 6. generalized clonic seizure with loss of posture reflex. Generalized seizure with loss of posture reflex. Observation lasted 10 minutes.\u003c/p\u003e\u003cp\u003e\u003cb\u003eExperiment 2: Electrocorticographic recording (EcoG) Surgery for electrode implantation.\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe animals were anaesthetized by intraperitoneal injection of a combination of 10% ketamine hydrochloride at a dose of 100 mg / kg i.p. and 2% xylazine hydrochloride at a dose of 5 mg / kg i.p. The degree of anaesthesia depth was assessed using the pain reflex after the interdigital reflex test. In cases where the reflex was still present, anesthetic supplementation was carried out with 1/4 of the dose initially applied. During anesthesia, the animals were positioned in a stereotactic apparatus for trichotomy of the head and application of the local anesthetic Lidocaine to the incised skin.\u003c/p\u003e\u003cp\u003eAfter asepsis with alcoholic iodopolivinylpyrolidone (PVPI) O, surgery was performed to expose the skull. Two bilateral holes were drilled into the rat's skull with a dental drill. Activated silver electrodes (0.5 mm diameter tip exposure) were placed in the dura mater above the frontal cortex at breech coordinates \u0026minus;\u0026thinsp;0.96 mm and \u0026plusmn;\u0026thinsp;1.0 mm lateral (Hamoy et al., \u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e2018\u003c/span\u003e) in the motor cortex region. A reinforcement screw was fixed to the skull, and the electrodes were fixed with dental acrylic cement (self-curing acrylic).\u003c/p\u003e\n\u003ch3\u003eElectrocorticographic recording\u003c/h3\u003e\n\u003cp\u003eFive days after the electrodes were implanted, the groups were administered the drugs according to the following design: a) Control group (received the equivalent volume of 0.9% saline solution); b) group treated with pentylenetetrazole 60 mg/kg i.p. ;\u003c/p\u003e\u003cp\u003ec) Group treated with camphorated oil 470 mg/kg i.p. The recordings lasted 30 minutes and nine animals were used for each experiment.\u003c/p\u003e\u003cp\u003eThe recordings were evaluated in terms of their power in oscillations up to 40 Hz, including the following ranges of brain oscillations: Delta (1\u0026ndash;4 Hz), Theta (4\u0026ndash;8 Hz), Alpha (8\u0026ndash;12 Hz), Beta (12\u0026ndash;28 Hz) and Gamma (28\u0026ndash;40 Hz).\u003c/p\u003e\n\u003ch3\u003eExperiment 3 - Performance of anticonvulsants\u003c/h3\u003e\n\u003cp\u003eAnticonvulsant drugs were tested after the application of camphor 470 mg/kg i.p., the clinical trial was carried out with three different anticonvulsants: Phenobarbital (10 mg/kg i.p.), Phenytoin (10 mg/kg i.p.), Diazepam (10 mg/kg i.p.), Sodium valproate (10 mg/kg i.p.), Propofol (10 mg/kg i.v.) lateral tail vein.\u003c/p\u003e\u003cp\u003eTo assess the control of seizures by anticonvulsants, the animals were treated with camphor followed by the administration of anticonvulsants; the recordings lasted 15 minutes. N\u0026thinsp;=\u0026thinsp;9 was used for each group.\u003c/p\u003e\u003cdiv id=\"Sec8\" class=\"Section2\"\u003e\u003ch2\u003eAnalysis of the recordings\u003c/h2\u003e\u003cp\u003eThe recordings were obtained using a differential amplifier with high AC input impedance (Grass Technologies, Model P511) adjusted with 0.3 HZ and 3 KHz filtering, monitored with an oscilloscope (Protek, Model 6510) and continuously digitized at a rate of 1 KHz by a computer equipped with a data acquisition card (National Instruments, Austin, TX).\u003c/p\u003e\u003cp\u003eThe seizures were characterized using the Python programming language (version 2.7) and the Signal \u0026reg; 3.0 program. For signal acquisition. Analyses were carried out at a frequency of up to 40 Hz and divided into bands according to Hamoy et al. (\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e2018\u003c/span\u003e) into Beta (1\u0026ndash;4 Hz), Theta (4\u0026ndash;8 Hz), Alpha (8\u0026ndash;12), Beta (12\u0026ndash;28) and Gamma (28\u0026ndash;40 Hz), to interpret the dynamics during seizure development.\u003c/p\u003e\u003c/div\u003e\n\u003ch3\u003eEuthanasia\u003c/h3\u003e\n\u003cp\u003eAfter carrying out the experiments, the animals were euthanized with a lethal injection of 300mg/kg i.p. of ketamine associated with 30 mg/kg i.p. of xylazine hydrochloride and 10 mg/kg i.p. of diazepam; this procedure was necessary to avoid suffering the animals.\u003c/p\u003e\u003cdiv id=\"Sec10\" class=\"Section2\"\u003e\u003ch2\u003eStatistical analysis\u003c/h2\u003e\u003cp\u003eThe results were submitted to descriptive statistics, such as mean and standard deviation. One-way analysis of variance (ANOVA) was used, followed by a Tukey test. A significance index of *p\u0026thinsp;\u0026lt;\u0026thinsp;0.05 was adopted. **p\u0026thinsp;\u0026lt;\u0026thinsp;0.01, ***p\u0026thinsp;\u0026lt;\u0026thinsp;0.001.\u003c/p\u003e\u003c/div\u003e"},{"header":"Results","content":"\u003cp\u003e\u003cb\u003eDescription of the characteristic behavior related to Camphor-induced convulsion.\u003c/b\u003e\u003c/p\u003e\u003cp\u003eAfter the administration of camphorated oil, the animals showed a rapid evolution of the seizure, displaying six behaviors. The change in behavior begins with immobility (akinesia) with an average latency of 78.89\u0026thinsp;\u0026plusmn;\u0026thinsp;18.73 s, followed by head and neck tremors\u003c/p\u003e\u003cp\u003e(135.9\u0026thinsp;\u0026plusmn;\u0026thinsp;17.03 s) and tail stiffening (178\u0026thinsp;\u0026plusmn;\u0026thinsp;17.59 s). The next behaviour involves the somatic system more intensely, revealing itself as clonias of the forelimbs (219.6\u0026thinsp;\u0026plusmn;\u0026thinsp;37.37 s), the crisis evolving into a generalized clonic convulsion without loss of the posture reflex (281\u0026thinsp;\u0026plusmn;\u0026thinsp;38.70 s) and with a worsening of the crises causing generalized clonic convulsions with loss of the posture reflex (384.9\u0026thinsp;\u0026plusmn;\u0026thinsp;74.41 s). Each component of the behaviors recorded above is part of the subsequent behaviors.\u003c/p\u003e\u003cdiv id=\"Sec12\" class=\"Section2\"\u003e\u003ch2\u003eInsert Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e about here\u003c/h2\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eThere are reports in the literature of \u003cem\u003eCinnamomum camphora\u003c/em\u003e inducing or provoking tonic-clonic seizures in patients with and without a history of seizures. Camphor induced generalized tonic-clonic seizures in 95% of the cases in a study with human patients with no previous history of seizures (p\u0026thinsp;=\u0026thinsp;22) and caused generalized tonic- clonic seizures in 27.3% and localized or bilateral seizures in 15% of the other group of patients in the study, with a history of epilepsy or drug seizures (p\u0026thinsp;=\u0026thinsp;33) (Mathew et al, 2021).\u003c/p\u003e\u003cp\u003e\u003cb\u003eCamphor-induced seizures have similar characteristics to the PTZ-induced chemoconvulsant model.\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe readings of the ECoG recordings for the control group showed low amplitudes (0.05 mV), which keeps the recording regular, so the spectrogram shows higher levels of energy distributed at frequencies below 10 Hz (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eA and B). The amplification of the recording shows morphographic components related to the activity of the rat's motor cortex, characterized by low amplitude with a predominance of low-frequency brain oscillations (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eC).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec13\" class=\"Section2\"\u003e\u003ch2\u003eInsert Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e about here\u003c/h2\u003e\u003cp\u003eThe PTZ-treated group showed changes in the ECoG tracing with cyclic activity reaching amplitudes above 0.5 mV during ictal status, which are characteristic of seizures, and interictal status, which was characterized by a decrease in the amplitude of the recording to the range of 0.05 mV. After the application of PTZ, the latency for the onset of seizures was 48\u0026thinsp;\u0026plusmn;\u0026thinsp;24s. The frequency spectrogram shows an increase in power in oscillations distributed up to 40 Hz (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eA).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eThe group treated with camphor oil showed intercalated electrocorticographic tracings between ictal and interictal status, characterized by neuronal recruitment with an increase in field potential during ictal status, which showed an increase in the power of the recordings, and interictal status with a decrease in power; the latency period for occurrence was 78.89\u0026thinsp;\u0026plusmn;\u0026thinsp;18.73 s. The spectrogram shows a greater distribution of energy during ictal status (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eB).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec14\" class=\"Section2\"\u003e\u003ch2\u003eInsert Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e about here\u003c/h2\u003e\u003cp\u003eThe spectral power distribution revealed greater amplitude in all brain waves in the camphor and PTZ groups compared to the control group (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eA). Power in the delta, theta and alpha frequencies was greater in the PTZ group, while gamma oscillations were more powerful in the camphor group. In this way, the average power in linear oscillations up to 40 Hz for the control group was 0.366\u0026thinsp;\u0026plusmn;\u0026thinsp;0.086 mV\u0026sup2; / Hz x 10-\u0026sup3; was lower than the groups treated with PTZ 9.11\u0026thinsp;\u0026plusmn;\u0026thinsp;1.78 mV\u0026sup2; / Hz x 10-\u0026sup3; and camphorated oil\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e6.46\u0026thinsp;\u0026plusmn;\u0026thinsp;1.77 mV\u0026sup2; / Hz x 10-\u0026sup3; (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eB).\u003c/p\u003e\u003cp\u003eMean analyses of the delta, theta, alpha, beta and gamma brain frequency bands showed an increase compared to the control group. Thus, PTZ and camphorated oil were able to induce disturbances in all brain waves, but the animals treated with camphorated oil showed greater power in gamma oscillations compared to the PTZ group (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eA).\u003c/p\u003e\u003cp\u003eFor delta oscillations, the control group had a lower mean power (0.109\u0026thinsp;\u0026plusmn;\u0026thinsp;0.022 mV\u0026sup2; / Hz x 10-\u0026sup3;) than the other groups PTZ (0.814\u0026thinsp;\u0026plusmn;\u0026thinsp;0.171 mV\u0026sup2; / Hz x 10-\u0026sup3;) and camphorated oil (0.271\u0026thinsp;\u0026plusmn;\u0026thinsp;0.0620 mV\u0026sup2; / Hz x 10-\u0026sup3;). The group treated with PTZ had a higher mean than the group treated with camphorated oil (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eC).\u003c/p\u003e\u003cp\u003eThe mean power in theta oscillations for the control group (0.723\u0026thinsp;\u0026plusmn;\u0026thinsp;0.017 mV\u0026sup2; / Hz x 10-\u0026sup3;) was lower than the PTZ (2.141\u0026thinsp;\u0026plusmn;\u0026thinsp;0.263 mV\u0026sup2; / Hz x 10-\u0026sup3;) and camphorated oil (0.321\u0026thinsp;\u0026plusmn;\u0026thinsp;0.048 mV\u0026sup2; / Hz x 10-\u0026sup3;) groups. The group treated with PTZ was superior to the group treated with camphorated oil (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eD).\u003c/p\u003e\u003cp\u003eIn alpha oscillations, the control group had a mean of 0.033\u0026thinsp;\u0026plusmn;\u0026thinsp;0.007 mV\u0026sup2;/Hz x 10-\u003c/p\u003e\u003cp\u003e\u0026sup3;, which was lower than the groups treated with PTZ (2.35\u0026thinsp;\u0026plusmn;\u0026thinsp;0.51 mV\u0026sup2;/Hz x 10-\u0026sup3;) and camphorated oil (0.60\u0026thinsp;\u0026plusmn;\u0026thinsp;0.153 mV\u0026sup2;/Hz x 10-\u0026sup3;). The group treated with camphorated oil was inferior to the group treated with PTZ (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eE).\u003c/p\u003e\u003cp\u003eFor beta oscillations, the control group had a mean of 0.013\u0026thinsp;\u0026plusmn;\u0026thinsp;0.006 mV\u0026sup2; / Hz x 10-\u0026sup3;, which was lower than the treated groups. The PTZ group had a mean power of 2.13\u003c/p\u003e\u003cp\u003e\u0026plusmn;\u0026thinsp;0.354 mV\u0026sup2;/Hz x 10-\u0026sup3; and was similar to the group treated with camphorated oil (p\u0026thinsp;=\u0026thinsp;0.1928) (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eF).\u003c/p\u003e\u003cp\u003eFor gamma oscillations, the control group (0.0059\u0026thinsp;\u0026plusmn;\u0026thinsp;0.0011 mV\u0026sup2;/Hz x 10-\u0026sup3;) was lower than the other treated groups. The camphor oil-treated group (0.730\u0026thinsp;\u0026plusmn;\u0026thinsp;0.141 mV\u0026sup2;/Hz x 10-\u0026sup3;) was superior to the PTZ-treated group (0.471\u0026thinsp;\u0026plusmn;\u0026thinsp;0.141 mV\u0026sup2;/Hz x 10-\u0026sup3;) (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eG).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec15\" class=\"Section2\"\u003e\u003ch2\u003eInsert Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e about here\u003c/h2\u003e\u003cp\u003eThe spectral distribution graph of power at frequencies up to 40 Hz in the recordings of camphorated oil-induced seizures was divided into two distinct periods during the recording that occur in a cyclical manner, in the ictal status period a higher level of power was observed and in the interictal status period a lower level of power was observed in the oscillations (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eA). The average power for the control group (0.366\u0026thinsp;\u0026plusmn;\u0026thinsp;0.086 mV\u0026sup2;/Hz x 10-\u0026sup3;) was similar to the interictal status group (p\u0026thinsp;=\u0026thinsp;0.075). However, all groups had lower mean power during ictal status (10.25\u0026thinsp;\u0026plusmn;\u0026thinsp;1.661 mV\u0026sup2;/Hz x 10-\u0026sup3;) (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eB).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eFor delta oscillations (1\u0026ndash;4 Hz), the control group (0.109\u0026thinsp;\u0026plusmn;\u0026thinsp;0.022 mV\u0026sup2;/Hz x 10-\u0026sup3;) had a mean power similar to the interictal period (p\u0026thinsp;=\u0026thinsp;0.092), but was lower than the camphor oil (0.271\u0026thinsp;\u0026plusmn;\u0026thinsp;0.062 mV\u0026sup2;/Hz x 10-\u0026sup3;) and ictal status (0.351\u0026thinsp;\u0026plusmn;\u0026thinsp;0.081 mV\u0026sup2;/Hz x 10-\u0026sup3;) groups. The ictal status group was superior to all groups (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eC).\u003c/p\u003e\u003cp\u003eThe theta oscillations in the status ictal period (0.433\u0026thinsp;\u0026plusmn;\u0026thinsp;0.114 mV\u0026sup2;/Hz x 10-\u0026sup3;) were higher than in the other groups. The interictal period had a higher mean (0.197\u0026thinsp;\u0026plusmn;\u0026thinsp;0.100 mV\u0026sup2;/Hz x 10-\u0026sup3;) than the control group (0.072\u0026thinsp;\u0026plusmn;\u0026thinsp;0.017 mV\u0026sup2;/Hz x 10-\u0026sup3;) (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eD).\u003c/p\u003e\u003cp\u003eFor alpha oscillations, the mean power during ictal status (0.872\u0026thinsp;\u0026plusmn;\u0026thinsp;0.308 mV\u0026sup2;/Hz x 10-\u0026sup3;) was higher than in the other groups. The average power for interictal status (0.225\u0026thinsp;\u0026plusmn;\u0026thinsp;0.063 mV\u0026sup2;/Hz x 10-\u0026sup3;) was similar to the control group (p\u0026thinsp;=\u0026thinsp;0.1129) (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eE).\u003c/p\u003e\u003cp\u003eThe average power of beta oscillations during the ictal status period (3.18\u0026thinsp;\u0026plusmn;\u0026thinsp;0.364 mV\u0026sup2;/Hz x 10-\u0026sup3;) was higher than the other groups. The control group (0.013\u0026thinsp;\u0026plusmn;\u0026thinsp;0.0065 mV\u0026sup2;/Hz x 10-\u0026sup3;) was lower than the interictal status period (0.762\u0026thinsp;\u0026plusmn;\u0026thinsp;0.212 mV\u0026sup2;/Hz x 10\u0026sup3;) (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eF).\u003c/p\u003e\u003cp\u003eFor gamma oscillations, the control group (0.005\u0026thinsp;\u0026plusmn;\u0026thinsp;0.001 mV\u0026sup2;/Hz x 10-\u0026sup3;) was lower than the other groups. The average power for the ictal status period (1.20\u0026thinsp;\u0026plusmn;\u0026thinsp;0.25 mV\u0026sup2;/Hz x 10-\u0026sup3;) was higher than the other groups. The camphorated oil group (0.73\u0026thinsp;\u0026plusmn;\u0026thinsp;0.141 mV\u0026sup2;/Hz x 10-\u0026sup3;) was superior to the interictal status group (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eG).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec16\" class=\"Section2\"\u003e\u003ch2\u003eInsert Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e about here\u003c/h2\u003e\u003cp\u003e\u003cb\u003eCamphor had a greater preponderance in the power of beta and gamma oscillations and was refractory to treatment with phenobarbital and phenytoin.\u003c/b\u003e\u003c/p\u003e\u003cp\u003eTo assess seizure control observed after camphorated oil treatment, the animals were treated with anticonvulsants, and then ECoGs were recorded, and their power in beta (12\u0026ndash;28 Hz) and gamma (28\u0026ndash;40 Hz) oscillations was assessed. Seizure control after phenobarbital use was observed 10 minutes after recording. During the first 10 minutes, periods of ictal and interictal states could still be identified (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003eA).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003ePhenytoin and sodium valproate were ineffective in controlling seizures, with the ictal and interictal states maintained throughout the recording, demonstrating that the chemoconvulsant model induced by camphorated oil was refractory to the treatments (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003eB and D). Seizure control was effectively achieved with diazepam and propofol, which indicated better protection for the animals (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003eC and E), with propofol being more effective. Recording patterns obtained with the use of anticonvulsants are shown for beta and gamma oscillations (Figs.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003eF and G).\u003c/p\u003e\u003cp\u003eThe mean beta oscillation in the control group was 0.013\u0026thinsp;\u0026plusmn;\u0026thinsp;0.006 mV\u0026sup2;/Hz x 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e, similar to the group treated with Diazepam (p\u0026thinsp;=\u0026thinsp;0.959) and propofol (p\u0026thinsp;=\u0026thinsp;0.999), but was lower than the other groups. The group treated with phenytoin (1.41\u0026thinsp;\u0026plusmn;\u0026thinsp;0.29 mV\u0026sup2;/Hz x 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e) was superior to the other groups treated with anticonvulsants. The group treated with phenobarbital (0.659\u0026thinsp;\u0026plusmn;\u0026thinsp;0.178 mV\u0026sup2;/Hz x 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e) and lower than the camphorated oil group, treated with phenytoin and sodium valproate. The diazepam-treated group (0.0942\u0026thinsp;\u0026plusmn;\u0026thinsp;0.0193 mV\u0026sup2;/Hz x 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e was similar to the propofol-treated group (p\u0026thinsp;=\u0026thinsp;0.999) (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003eF). For gamma oscillations, the control group (0.005\u0026thinsp;\u0026plusmn;\u0026thinsp;0.0011 mV\u0026sup2;/Hz x 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e) was similar to the diazepam group (p\u0026thinsp;=\u0026thinsp;0.998) and the propofol-treated group (p\u0026thinsp;=\u0026thinsp;999). The phenobarbital group (0.523\u0026thinsp;\u0026plusmn;\u0026thinsp;0.128 mV\u0026sup2;/Hz x 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e) was similar to the phenytoin group (p\u0026thinsp;=\u0026thinsp;0.923) and the sodium valproate-treated group (p\u0026thinsp;=\u0026thinsp;0.169), but was inferior to the camphor group. The phenytoin-treated group (0.471\u0026thinsp;\u0026plusmn;\u0026thinsp;0.094 mV\u0026sup2;/Hz x 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e) was similar to the group treated with sodium valproate (p\u0026thinsp;=\u0026thinsp;0.787). The group treated with Diazepam (0.0196\u0026thinsp;\u0026plusmn;\u0026thinsp;0.004 mV\u0026sup2;/Hz x 10\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003e) was similar to the group treated with propofol (p\u0026thinsp;=\u0026thinsp;0.999) (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003eG).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec17\" class=\"Section2\"\u003e\u003ch2\u003eInsert Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e about here\u003c/h2\u003e\u003c/div\u003e"},{"header":"Discussion","content":"\u003cp\u003eThe behavioral data showed that camphor presented rapid evolution of convulsive behavior, six behaviors were identified culminating in generalized clonic convulsion with loss of the posture reflex (384.9\u0026thinsp;\u0026plusmn;\u0026thinsp;74.41 s) after application. The behaviours presented the following components: akinesia, head and neck tremor, tail stiffening, forelimb clonias, generalized clonic seizures without loss of posture reflex and generalized clonic seizures with loss of posture reflex, with the subsequent behaviours presenting components of the previous behaviours. These data indicate possible points of intersection between the chemoconvulsant model promoted by the administration of camphor and models traditionally studied with other chemical agents such as pentylenetetrazole and kainic acid, which represent suitable models for the study of refractory epilepsy to screen the activity of anticonvulsant drugs and which help to clarify the mechanisms involved in convulsive and epileptic processes (Taylor et al., 1981; L\u0026uuml;ttjohann et al., \u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e2009\u003c/span\u003e; Van Erum et al.,2019; Ishikawa et al., \u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e2020\u003c/span\u003e; de Almeida et al., \u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e2020\u003c/span\u003e; Sing et al., 2021; Alharbi, \u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2021\u003c/span\u003e; Dos Santos et al., \u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e2021\u003c/span\u003e; Torun, Kilinc \u0026amp; Kilinc, 2022). Although some differences can be noted, such as the latency for the onset of seizure behavior and the dose, which shows that PTZ is more potent in triggering seizures when compared to camphor. A study by Hamoy et al. (\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e2018\u003c/span\u003e) demonstrated the activity of the cequisterpene cunaniol obtained from wild Clibadium as a powerful GABA antagonist chemoconvulsant with greater potency than PTZ, indicating that natural substances can be used as new convulsive models.\u003c/p\u003e\u003cp\u003eIn the electrocorticogram, the morphographic elements showed a similarity to the recrudescence of activity that increases the field potential recorded, characterized as a salvo of potentials that results in the ictal period, which is characterized by high power in the recording, followed by a decrease in power corresponding to the post-ictal period; these morphographic characteristics can be observed in both camphor-induced and PTZ- induced seizures. This characteristic was also observed for cunaniol and caffeine-induced seizures (Hamoy et al. \u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e2018\u003c/span\u003e; Eir\u0026oacute;-Quirino et al., \u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e2024\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eIn general, there is an increase in the power of brain oscillations up to 40 Hz during camphor- and PTZ-induced seizures when compared to the control group. The breakdown of brain oscillations indicates that Delta (1\u0026ndash;4 Hz), Theta (4-8Hz) and Alpha (8\u0026ndash;12 Hz) powers were higher in the PTZ-treated group, but beta (12\u0026ndash;28 Hz) oscillations were similar, and gamma power was higher for camphor, indicating a preponderance of forces in seizures induced at frequencies of (28-40Hz). Leit\u0026atilde;o et al., (\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e2022\u003c/span\u003e) studying seizures provoked by thiocolchicoside in rats observed an increase in the powers of cerebral oscillations in the motor cortex during the ictal period. These effects were also observed for seizures induced by nicotine and pilocarpine (Da Silva et al., \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e2022\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eRegarding the characteristics of camphor-induced seizures, ictal and inter-ictal periods were observed. During the ictal period, the average power of the brain oscillations increased towards Beta and Gamma oscillations, with a characteristic higher frequency between (12\u0026ndash;40 Hz). According to Naggar et al. (\u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e2020\u003c/span\u003e) field potential oscillations reflect repetitive firing of neuronal synaptic activity in certain areas of the brain that can be distinguished as slow (alpha, delta and theta), fast (beta and gamma). In this way, during the ictal period caused by camphor, fast powers predominate. These ictal characteristics have also been observed in the outbreak-suppression that occurs in children with West syndrome (Nariai et al., \u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e2017\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eWhen using anticonvulsants such as phenobarbital, phenytoin, diazepam, sodium valproate and propofol to control seizures and observing after treatment with camphor in the reduction of average power in beta and gamma oscillations. The best efficacy was observed with the application of diazepam which positively modulates the response to GABA, as well as binding to the allosteric site of the GABAA receptor which results in an increase in the frequency of opening of the Cl\u003csup\u003e-\u003c/sup\u003e channel when GABA is present, hyperpolarizing the cell and making it difficult to fire the action potential., reducing the average power in beta and gamma. Zhou et al. (\u003cspan citationid=\"CR52\" class=\"CitationRef\"\u003e2021\u003c/span\u003e) demonstrated through a randomized clinical trial, with seizure models using lithium and pilocarpine in adult rats, that diazepam, when administered within 10 minutes of seizure onset, has better results through global suppression of power. Similarly, Rojas et al. (\u003cspan citationid=\"CR42\" class=\"CitationRef\"\u003e2018\u003c/span\u003e) demonstrated seizure interruption, but power in the ~\u0026thinsp;20\u0026ndash;70 Hz bands (beta and gamma) returned hours later in rats with a seizure model using diisopropylfluorophosphate.\u003c/p\u003e\u003cp\u003ePhenobarbital was more effective at controlling average beta power when compared to the phenytoin group, this can be justified by the affinity with a specific site of GABAA receptors, causing a prolongation of the opening time of these channels, resulting in a greater influx of Cl\u003csup\u003e-\u003c/sup\u003e, as well as a reduction in the action of AMPA receptors and the blocking of L and N type Ca\u003csup\u003e2+\u003c/sup\u003e channels. This corroborates other studies involving the control of seizures induced by pro-convulsant substances where treatment with diazepam was very effective (Mandhane et al., \u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e2007\u003c/span\u003e; Akula et al., \u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e2009\u003c/span\u003e; Mikutra-Cencora \u0026amp; Teitelbaum, \u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e2025\u003c/span\u003e). As was Propofol because it also binds to the allosteric site of the GABAA receptor, it has the particularity of a GABA-mimetic effect at higher concentrations, as well as the inhibition of NMDA receptors, breaking the cycle of hyperexcitability.\u003c/p\u003e\u003cp\u003eAlthough phenytoin delays the reactivation of Na\u003csup\u003e+\u003c/sup\u003e channels in a use-dependent manner by inhibiting the most active neurons during seizures, in our study it did not show adequate efficacy in the ictal and interictal states, which may be explained by refractory blockades and alternative triggering pathways, such as Ca channels and glutamate receptors. Furthermore, changes in pH and extracellular ions during the ictal state can alter the kinetics of Na\u003csup\u003e+\u003c/sup\u003e channels, reducing the affinity of phenytoin, corroborating the study by West et al. (\u003cspan citationid=\"CR50\" class=\"CitationRef\"\u003e2022\u003c/span\u003e) who observed that phenytoin was not effective in suppressing resistance seizures, as well as in the interictal period.\u003c/p\u003e\u003cp\u003eAs well as sodium valproate, which has a similar action in the context of blocking voltage-dependent Na\u003csup\u003e+\u003c/sup\u003e channels, together with the stimulation of glutamate decarboxylase and blocking of T-type Ca\u003csup\u003e2+\u003c/sup\u003e channels, In this context, Richardson, Petrou and Bryson (\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e2024\u003c/span\u003e) further highlighted that it is possible that it can increase the synaptic release of GABA, as well as modulate metabolic pathways, through the inhibition of alpha-ketoglutarate, increasing activity through the diversion of GABA.\u003c/p\u003e\u003cp\u003eIn the present study, we describe the behavioral and electrophysiological characteristics during seizures provoked by the application of Camphor, using epidural ECoG recordings in the motor cortex of rats. Treatment with camphor resulted in changes in the graph-elements of the recording, which exhibited characteristics of convulsant activity, confirming that camphor is a potent convulsant agent in the rat brain. The induced seizures show a cyclical pattern with cellular recruitment triggering a salvo of potentials in the ictal and post-ictal periods, with a preponderance of beta and gamma oscillations during the ictal period. Control of camphor-induced seizures was better achieved with diazepam and propofol. In this way, the use of camphor makes it a suitable model for studies that address the mechanism involved in neurotransmission to assess the refractoriness of anticonvulsant drugs.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAuthor Contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003ePriscille Fidelis Pacheco Hartcopff, Mois\u0026eacute;s Hamoy contributed to the conception, design of the study and review of the final manuscript. Clarissa Araujo da Paz, Luciana Eir\u0026oacute; Quirino, Thaysa de Sousa Reis, Daniella Bastos de Araujo, Axell Lins contributed to the experimental design and original text. Rafael dos Reis de Souza, Caio Gabriel da Silva Motta, Mateus Felipe Ferreira Ara\u0026uacute;jo, Marcelo Victor dos Santos Brito, Murilo Farias dos Santos, Alisson Bruno Leite Lima, Mois\u0026eacute;s Hamoy contributed to the methodology implementation and data analysis.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe thank the Coordination for the Improvement of Higher Education Personnel, the Federal University of Par\u0026aacute; and the Institute of Biological Sciences\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eData availability\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe datasets generated are available from the corresponding author on reasonable request.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors have nothing to report.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics statement\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll procedures were approved by Animal Use Ethics Committee of the Federal University of Par\u0026aacute; (CEUA/UFPA) N\u0026ordm;\u0026nbsp;8381060818 (ID 000975).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent to participate/Consent for publication\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConflict of interest statement\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eAkula KK, Dhir A, Kulkarni SK (2009) Effect of various antiepileptic drugs in a pentylenetetrazol-induced seizure model in mice. \u003cem\u003eMethods Find Exp Clin Pharmacol\u003c/em\u003e 31(7):423\u0026ndash;432. doi: 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This study investigated the neurobehavioral and electrocorticographic characteristics of seizures induced by camphorated oil in Wistar rats and compared them to those triggered by pentylenetetrazol (PTZ), a classical chemoconvulsant model. Male rats received intraperitoneal administration of camphor (470 mg/kg), and behavioral evolution was monitored to characterize seizure onset and progression. Electrocorticographic (ECoG) recordings from the motor cortex were acquired for 30 minutes to assess cortical oscillatory activity during ictal and interictal periods. Camphor administration elicited six distinct and rapidly evolving behavioral patterns culminating in generalized clonic seizures with loss of postural reflexes. ECoG analyses revealed cyclic high-power ictal discharges interspersed with low-power interictal activity, resembling PTZ-induced seizures but with lower potency. Evaluation of anticonvulsant efficacy demonstrated that camphor-induced seizures were refractory to phenobarbital and phenytoin, whereas diazepam and propofol significantly reduced β- and γ-band power and effectively controlled convulsive activity. These findings suggest that camphor produces a reproducible chemoconvulsant model characterized by prominent cortical excitability and partial pharmacoresistance, supporting its potential utility in neurotoxicological and neuropharmacological studies aimed at understanding seizure mechanisms and testing antiepileptic drug responsiveness.\u003c/p\u003e","manuscriptTitle":"Potential use of Camphor as a new chemoconvulsant model Camphor-induced seizures in rats","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-11-07 06:44:25","doi":"10.21203/rs.3.rs-7895031/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2026-02-09T00:13:17+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-12-15T08:29:42+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"93319943048623194340464608880568592797","date":"2025-11-25T16:03:20+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"212954965418680960768772173703974383698","date":"2025-11-25T12:12:16+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-07T19:43:14+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"61191881702957357002685674157526205579","date":"2025-10-27T19:06:28+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-10-27T18:09:03+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-10-19T23:46:55+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-10-19T23:46:35+00:00","index":"","fulltext":""},{"type":"submitted","content":"Neurotoxicity Research","date":"2025-10-18T17:56:55+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"neurotoxicity-research","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"ntre","sideBox":"Learn more about [Neurotoxicity Research](http://bacandrology.biomedcentral.com/)","snPcode":"12640","submissionUrl":"https://submission.nature.com/new-submission/12640/3","title":"Neurotoxicity Research","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"d13fd820-ae03-4da8-8648-9c1caf1502b3","owner":[],"postedDate":"November 7th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2026-03-30T16:22:44+00:00","versionOfRecord":{"articleIdentity":"rs-7895031","link":"https://doi.org/10.1007/s12640-026-00793-3","journal":{"identity":"neurotoxicity-research","isVorOnly":false,"title":"Neurotoxicity Research"},"publishedOn":"2026-03-24 16:10:24","publishedOnDateReadable":"March 24th, 2026"},"versionCreatedAt":"2025-11-07 06:44:25","video":"","vorDoi":"10.1007/s12640-026-00793-3","vorDoiUrl":"https://doi.org/10.1007/s12640-026-00793-3","workflowStages":[]},"version":"v1","identity":"rs-7895031","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7895031","identity":"rs-7895031","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}