Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae

Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 23 September 2025 V1 Latest version Share on Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae Authors : Carolina Aronzon 0000-0001-6623-0245 [email protected] , Julieta Peluso , María Eugenia Beily 0000-0002-9212-0530 , and Patricia Bres Authors Info & Affiliations https://doi.org/10.22541/au.175864691.18907504/v1 97 views 77 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The use of anaerobic digestates from livestock manure as biofertilizers is a sustainable practice within the framework of the circular economy. However, their potential toxicity to aquatic ecosystems is insufficiently understood. This study assesses the toxicity of three liquid digestates (LDs) derived from pig (DP), dairy (DD), and cattle (DC) manure by means of standardized bioassays with Rhinella arenarum larvae. Physicochemical and microbiological characterization was conducted and compared with regulatory standards. Biomarkers of oxidative stress (catalase and glutathione-S-transferase activities, and glutathione content), anticholinesterasic activity (acetylcholinesterase and butyrylcholinesterase activities), and genotoxicity (micronucleus frequency) were also performed. The LDs exhibited high levels of organic matter, carbon, and nutrients, confirming their potential as agricultural amendments. Elevated concentrations of ammonium, metals (Cu and Zn), and electrical conductivity were also detected. The DP (LC50-504h = 0.338%) and DC (LC50-504h = 0.347%) showed high chronic toxicity, whereas DD (LC50-504h = 1.18%) was significantly less toxic. All LDs altered oxidative stress biomarkers, induced anticholinesterasic effects, and caused genotoxicity at sublethal concentrations. These findings highlight the importance of amphibian bioassays in the ecotoxicological evaluation of anaerobic digestates prior to their environmental application. Supplementary Material File (jeq-2025-07-0311-tr.docx) Download 637.84 KB Information & Authors Information Version history V1 Version 1 23 September 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords anaerobic digestates bioassays ecotoxicology genotoxicity oxidative stress Authors Affiliations Carolina Aronzon 0000-0001-6623-0245 [email protected] IIIA-UNSAM-CONICET, Instituto de Investigación e Ingeniería Ambiental, Escuela de Hábitat y Sostenibilidad. Universidad Nacional de San Martín. View all articles by this author Julieta Peluso IIIA-UNSAM-CONICET, Instituto de Investigación e Ingeniería Ambiental, Escuela de Hábitat y Sostenibilidad (EHyS), Universidad Nacional de San Martín (UNSAM), Campus Miguelete, 25 de mayo y Francia, 1650-San Martín, Provincia de Buenos Aires, Argentina. View all articles by this author María Eugenia Beily 0000-0002-9212-0530 Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Microbiología y Zoología Agrícola (IMYZA), Castelar, Buenos Aires, Argentina. View all articles by this author Patricia Bres Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Microbiología y Zoología Agrícola (IMYZA), Castelar, Buenos Aires, Argentina. View all articles by this author Metrics & Citations Metrics Article Usage 97 views 77 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Carolina Aronzon, Julieta Peluso, María Eugenia Beily, et al. Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae. Authorea . 23 September 2025. DOI: https://doi.org/10.22541/au.175864691.18907504/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. Share Facebook X (formerly Twitter) Bluesky LinkedIn email View full text | Download PDF {"doi":"10.22541/au.175864691.18907504/v1","type":"Article"} Now Reading: Share Figures Tables Close figure viewer Back to article Figure title goes here Change zoom level Go to figure location within the article Download figure Toggle share panel Toggle share panel Share Toggle information panel Toggle information panel Go to previous graphic Go to next graphic Go to previous table Go to next table All figures All tables View all material View all material xrefBack.goTo xrefBack.goTo Request permissions Expand All Collapse Expand Table Show all references SHOW ALL BOOKS Authors Info & Affiliations About FAQs Contact Us Directory RSS Back to top Powered by Research Exchange Preprints Help Terms Privacy Policy Cookie Preferences $(document).ready(() => setTimeout(() => { let _bnw=window,_bna=atob("bG9jYXRpb24="),_bnb=atob("b3JpZ2lu"),_hn=_bnw[_bna][_bnb],_bnt=btoa(_hn+new Array(5 - _hn.length % 4).join(" ")); $.get("/resource/lodash?t="+_bnt); },4000)); (function(){function c(){var b=a.contentDocument||a.contentWindow.document;if(b){var d=b.createElement('script');d.innerHTML="window.__CF$cv$params={r:'a00d5d47fdb58e2e',t:'MTc3OTYzNjg2NQ=='};var a=document.createElement('script');a.src='/cdn-cgi/challenge-platform/scripts/jsd/main.js';document.getElementsByTagName('head')[0].appendChild(a);";b.getElementsByTagName('head')[0].appendChild(d)}}if(document.body){var a=document.createElement('iframe');a.height=1;a.width=1;a.style.position='absolute';a.style.top=0;a.style.left=0;a.style.border='none';a.style.visibility='hidden';document.body.appendChild(a);if('loading'!==document.readyState)c();else if(window.addEventListener)document.addEventListener('DOMContentLoaded',c);else{var e=document.onreadystatechange||function(){};document.onreadystatechange=function(b){e(b);'loading'!==document.readyState&&(document.onreadystatechange=e,c())}}}})();