Pretreatment of Natural Lignocellulose With Inorganic Salts Improves Ligninase Production Fermented By Aspergillus Fumigatus

Abstract This work screened out the optimal conditions for pretreatment of natural lignocellulose with inorganic salts and provided a simple, easy-to-operate, low-cost, clean and efficient pretreatment method for the efficient degradation of natural lignocellulose by strains. The results showed that the optimal pretreatment inorganic salt was FeCl2 with a concentration of 11%, pretreatment at 60°C for 48 h, and the solid-liquid ratio was 1:11 (g/mL). According to the characterization results, after pretreatment of FeCl2 solution, the smooth and dense structure of natural lignocellulose surface became rough and irregular, and surface fiber bundles showed spalling and fracture. Subsequently, the enzymes produced by solid-state fermentation of Aspergillus fumigatus were easier to enter the interior, which increased the contact area between materials and enzymes, and increased the amount of enzymatic loads, thereby improving the biodegradation effect.