The Quantitative Proteomics Analysis in Glioblastoma After HULC Silencing

Background: Glioblastoma multiforme (GBM) is a malignant intracranial tumor threatening patients' survival. The study aimed to find the menchanisms of lncRNA highly up-regulated in liver cancer (HULC) affecting GBM or involved signaling pathways, which may providing theoretical support for targeted therapy. Methods: : Two cell lines were constructed: HULC-small interfering RNA (siRNA) and negative control. Then qRT-PCR was operated to detect the transfection efficiency and quantitative proteomics based on mass spectrometry (MS) were conducted. Finally, the differentially expressed proteins were analyzed in gene ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Results: : The relative expression level of HULC in HULC-siRNA was significantly lower than that in NC detected by qRT-PCR. A total of 136 differentially expressed proteins were identified, including 24 down-regulated and 112 up-regulated. GO classification results illustrated that they were centralized in cellular or single-organism process and biological regulation in biological process, cell and organelle in cellular component, binding and catalytic activity in molecular function. KEGG pathway enrichment analysis indicated that some were sinificantly enriched, including tight junction, metabolic pathways and arachidonic acid metabolism. It was further discovered that PLA2G4A was down regulated obviously after HULC silencing. Conclusion: HULC changed the proteomics characteristics of GBM, including regulating the expression of PLA2G4A. This study provided a new perspective on the menchanisms and potential drug targets of GBM.