PHYTOCHROME INTERACTING FACTOR 7 moderates the activity and the cytosolic-nuclear shuttling of PHYTOCHROME A

PHYTOCHROME INTERACTING FACTOR 7 moderates the activity and the cytosolic-nuclear shuttling of PHYTOCHROME A | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article PHYTOCHROME INTERACTING FACTOR 7 moderates the activity and the cytosolic-nuclear shuttling of PHYTOCHROME A Karen Halliday, Mengke Zhou, Andrés Romanowski This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7819683/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract In nature, plants sense and navigate complex vegetative environments using the phytochrome (phy) photoreceptor-signaling system. The canopy adaptation strategy, elicited by phyA, and the shade avoidance syndrome, regulated by phyB, allow plants to adapt to diverse forms of vegetation shade. The phyA High Irradiance Response (HIR) mode, involving photocycle-coupled nuclear shuttling, is essential for detecting persistent far-red rich conditions typical of canopy or occlusive shade. Our research identifies PHYTOCHROME INTERACTION FACTOR 7 (PIF7) as a critical regulator of phyA function. We demonstrate PIF7ox can effectively block phyA action. In persistent shade, PIF7 and phyA both accumulate and interact within nuclear bodies, with binding facilitated through the prion-like domain of PIF7 and the C-terminal PAS-PAS domain of phyA. PIF7 also interacts with FHY1 and FHL chaperones, interfering with phyA cytosolic-nuclear shuttling. This strategic molecular targeting by PIF7 provides an effective strategy for modulating the phyA HIR in response to shade signals. Biological sciences/Plant sciences/Plant molecular biology Biological sciences/Plant sciences/Plant physiology Full Text Additional Declarations There is NO Competing Interest. Supplementary Files Zhouetal.supplementarytables.pdf Supplementary Tables Zhouetal.supplementaryFigs.docx Supplementary Figure S1-7 Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7819683","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":537702311,"identity":"4d089bbf-c832-47c5-bb91-dc06a6bf3789","order_by":0,"name":"Karen 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