miR-468-3p suppresses osteogenic differentiation of BMSCs by targeting Runx2 and inhibits bone formation

miR-468-3p suppresses osteogenic differentiation of BMSCs by targeting Runx2 and inhibits bone formation | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 17 July 2024 V1 Latest version Share on miR-468-3p suppresses osteogenic differentiation of BMSCs by targeting Runx2 and inhibits bone formation Authors : Tao Fang , Zhang Ranxi , Song Feng , Xueru Chu , Qin Fu , and Qianqian Wu [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.172123307.71225957/v1 Published Journal of Orthopaedic Surgery and Research Version of record Peer review timeline 277 views 126 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract An improved understanding of the molecular actions underpinning bone marrow stromal cell (BMSC) differentiation could highlight new therapeutics for osteoporosis (OP). Current evidence indicates that microRNAs (miRNAs) exert critical roles in many biological systems, including osteoblast differentiation. In this study, we examined miR-468-3p effects on osteogenic differentiation (OD). Distinct miR-468-3p reductions were identified during OD. MiR-468-3p also suppressed BMSC OD in gain- and loss-of-function assays, while it negatively regulated Runx2 as shown by molecular, protein, and bioinformatics approaches. When Runx2 was inhibited by small-interfering RNA (siRNA), the inhibitory effects of miR-468-3p toward BMSC osteogenesis were considerably reversed. Also, silenced miR-468-3p in ovariectomized (OVX) and sham mice augmented bone mass (BM) and bone formation (BF) and improved trabecular (Tb) microarchitecture. Therefore, miR-468-3p is a novel Runx2 regulator with key physiological action in BF and OD. Supplementary Material File (manuscript.docx) Download 3.60 MB Information & Authors Information Version history V1 Version 1 17 July 2024 Peer review timeline Published Journal of Orthopaedic Surgery and Research Version of Record 30 Dec 2024 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Authors Affiliations Tao Fang Qingdao Municipal Hospital Group View all articles by this author Zhang Ranxi Qingdao Municipal Hospital Group View all articles by this author Song Feng Qingdao Municipal Hospital Group View all articles by this author Xueru Chu Ocean University of China School of Medicine and Pharmacy View all articles by this author Qin Fu Shengjing Hospital of China Medical University View all articles by this author Qianqian Wu [email protected] Qingdao Municipal Hospital Group View all articles by this author Metrics & Citations Metrics Article Usage 277 views 126 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Tao Fang, Zhang Ranxi, Song Feng, et al. miR-468-3p suppresses osteogenic differentiation of BMSCs by targeting Runx2 and inhibits bone formation. Authorea . 17 July 2024. 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