Cell culture of stromal cell isolated from human endometrium in vitro

ObjectiveTo establish the purified cell cultures of stromal cell of endometrium in vitro . MethodsCollagenase digestion,trypsase digestion,light microscope,and immunocytochemical staining were used. Endometrium and endometriotic tissues were isolated, purified,and cultured.ResultsThe purity of stromal cells was 95% identified by cytokeratin and vimentin staining. ConclusionThe purified stromal cell cultured from endometrial and endometriotic tissues are obtained by centrifugation. The cellular model will be useful to investigate the pathogenesis and therapy of endometriosis.