The experimental study of the effects of mifepristone on adenomyotic cells in vitro

Objective To observe the effects of mifepristone on the cultured adenomyotic cells. Methods With MTT assay,the growth state of premary cell culture trom adenomyotic implants of 11 women with adenomyosis was studied.The cells of each case were deveded into foru groups,three treat ment groups(1.25mg/L,2.5mg/L,5.0mg/L mifepristone,respectively) and one control group.cultured for 52 hours before the OD value was determined.Results The OD values of 1.25mg/L,2.5mg/L,5.0mg/L mifepristone and control group were (0.219±0.044),0.185±0.038) and (0.262±0.068) respectively.The OD value of 5.0mg/L groups had significantly lower OD values than the control group(P0.01),and both 5.0mg/L and 2.5mg/L groups had segnificantly lower OD values than the control group(P0.01).The inhibition rate of cell growth in 2.5mg/L group (IR=29.4%) was 1.79 times as much as that in 1.25mg/L,group(IR=16.4%),while in 5.0mg/L group (IR=42.0%) it was 1.43 times as much as that in 2.5mg/L group.Conclusion Mifepristone could suppress the growth of adenomyotic cells in vitro in a dose dependent manner.