Calculation of specifity constants Γ for RUBISCO of different species from microcalorimetric data

Calculation of specifity constants Γ for RUBISCO of different species from microcalorimetric data | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Method Article Calculation of specifity constants Γ for RUBISCO of different species from microcalorimetric data Joachim Frank, Frank Müh This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7366542/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 01 Dec, 2025 Read the published version in Photosynthesis Research → Version 1 posted 14 You are reading this latest preprint version Abstract Based on earlier data concerning the reaction enthalpy \(\:{\varDelta\:}_{\text{r}}H\) of Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from spinach (Frank et al. ( 2000 ), Phys. Chem. Chem. Phys. 2: 1301–1304), it is shown that the specifity constant \(\:{\Gamma\:}\) , indicating the ability of RUBISCO to discriminate between CO 2 and O 2 as substrate, can be determined from an isothermal titration calorimetric (ITC) measurement of \(\:{\varDelta\:}_{\text{r}}H\) as a function of the substrate concentration ratio \(\:\rho\:=\left[\text{C}{\text{O}}_{2}\right]/\left[{\text{O}}_{2}\right]\) . The approach does not need any radioactive materials and might be a cost-effective alternative for RUBISCO engineering to screen for variants with a high sepcifity constant. Carbon dioxide enzyme activity isothermal titration calorimetry oxygen reaction enthalpy ribulose 1 5-bisphosphate carboxylase/oxygenase Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 01 Dec, 2025 Read the published version in Photosynthesis Research → Version 1 posted Editorial decision: Revision requested 12 Oct, 2025 Reviews received at journal 28 Sep, 2025 Reviews received at journal 23 Sep, 2025 Reviewers agreed at journal 21 Sep, 2025 Reviewers agreed at journal 19 Sep, 2025 Reviewers agreed at journal 18 Sep, 2025 Reviews received at journal 05 Sep, 2025 Reviewers agreed at journal 27 Aug, 2025 Reviewers agreed at journal 27 Aug, 2025 Reviewers agreed at journal 21 Aug, 2025 Reviewers invited by journal 21 Aug, 2025 Editor assigned by journal 21 Aug, 2025 Submission checks completed at journal 18 Aug, 2025 First submitted to journal 13 Aug, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7366542","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Method Article","associatedPublications":[],"authors":[{"id":507069119,"identity":"b787bb2c-6c46-46a1-a05c-7d851517d090","order_by":0,"name":"Joachim Frank","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Joachim","middleName":"","lastName":"Frank","suffix":""},{"id":507069121,"identity":"044759d2-b44c-4c2c-add1-d3a200bff0ee","order_by":1,"name":"Frank Müh","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAzUlEQVRIiWNgGAWjYBAC9gYGBsYGEEuC+YBhAzFaeA7AtbAlgLXwkKCFx4CROC3Sh589nMGwLbFfuudD4cw2Bnl7glr40swNNzDcTpw55+wGw41tDIY9hLTY8zCYST4AatlwI3eD4cM2BkaCWnh42L+Btey/kfMApMWeCC08ZpIgh22QyGEAOSyRGC1lkjMMbhvPuJFmYDjjnERyzwHCDtsm2VNxW7Z/RvIzw54yG9v2BkLWgIEBmGQDUhJEqYcD5gekqR8Fo2AUjIKRAgAw+j69kODk8QAAAABJRU5ErkJggg==","orcid":"","institution":"Johannes Kepler Universität Linz","correspondingAuthor":true,"prefix":"","firstName":"Frank","middleName":"","lastName":"Müh","suffix":""}],"badges":[],"createdAt":"2025-08-13 15:38:29","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7366542/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7366542/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s11120-025-01187-w","type":"published","date":"2025-12-01T15:57:50+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":97724588,"identity":"b4b1759b-2568-4a97-821a-499a62a495f1","added_by":"auto","created_at":"2025-12-08 16:12:47","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":437566,"visible":true,"origin":"","legend":"","description":"","filename":"RUBISCO140825.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7366542/v1_covered_7521cf5b-bca9-4f39-82b0-cec5844f26a3.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Calculation of specifity constants Γ for RUBISCO of different species from microcalorimetric data","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"photosynthesis-research","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"pres","sideBox":"Learn more about [Photosynthesis Research](http://link.springer.com/journal/11120)","snPcode":"11120","submissionUrl":"https://submission.nature.com/new-submission/11120/3","title":"Photosynthesis Research","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Carbon dioxide, enzyme activity, isothermal titration calorimetry, oxygen, reaction enthalpy, ribulose 1,5-bisphosphate carboxylase/oxygenase","lastPublishedDoi":"10.21203/rs.3.rs-7366542/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7366542/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBased on earlier data concerning the reaction enthalpy \u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\:{\\varDelta\\:}_{\\text{r}}H\\)\u003c/span\u003e\u003c/span\u003e of Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from spinach (Frank et al. (\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e2000\u003c/span\u003e), Phys. Chem. Chem. Phys. 2: 1301\u0026ndash;1304), it is shown that the specifity constant \u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\:{\\Gamma\\:}\\)\u003c/span\u003e\u003c/span\u003e, indicating the ability of RUBISCO to discriminate between CO\u003csub\u003e2\u003c/sub\u003e and O\u003csub\u003e2\u003c/sub\u003e as substrate, can be determined from an isothermal titration calorimetric (ITC) measurement of \u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\:{\\varDelta\\:}_{\\text{r}}H\\)\u003c/span\u003e\u003c/span\u003e as a function of the substrate concentration ratio \u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\:\\rho\\:=\\left[\\text{C}{\\text{O}}_{2}\\right]/\\left[{\\text{O}}_{2}\\right]\\)\u003c/span\u003e\u003c/span\u003e. The approach does not need any radioactive materials and might be a cost-effective alternative for RUBISCO engineering to screen for variants with a high sepcifity constant.\u003c/p\u003e","manuscriptTitle":"Calculation of specifity constants Γ for RUBISCO of different species from microcalorimetric data","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-08-29 16:16:09","doi":"10.21203/rs.3.rs-7366542/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-10-12T17:26:19+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-09-28T17:33:31+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-09-23T22:06:59+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"318896125958008402761494063463336721019","date":"2025-09-22T02:45:31+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"60163566776897929946206305896416359207","date":"2025-09-19T16:11:14+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"48808607592602703525177397761049447351","date":"2025-09-18T14:34:34+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-09-05T16:39:50+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"126503256504042074641074103685041070187","date":"2025-08-27T08:18:17+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"47210953518259452491030820167841993954","date":"2025-08-27T07:32:34+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"199294297638308228851066729278773478696","date":"2025-08-21T19:12:59+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-08-21T16:07:09+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-08-21T12:45:58+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-08-18T06:30:04+00:00","index":"","fulltext":""},{"type":"submitted","content":"Photosynthesis Research","date":"2025-08-13T15:35:24+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"photosynthesis-research","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"pres","sideBox":"Learn more about [Photosynthesis Research](http://link.springer.com/journal/11120)","snPcode":"11120","submissionUrl":"https://submission.nature.com/new-submission/11120/3","title":"Photosynthesis Research","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"7ec37b1a-068f-46b9-b82f-49d461e8404b","owner":[],"postedDate":"August 29th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2025-12-08T16:09:15+00:00","versionOfRecord":{"articleIdentity":"rs-7366542","link":"https://doi.org/10.1007/s11120-025-01187-w","journal":{"identity":"photosynthesis-research","isVorOnly":false,"title":"Photosynthesis Research"},"publishedOn":"2025-12-01 15:57:50","publishedOnDateReadable":"December 1st, 2025"},"versionCreatedAt":"2025-08-29 16:16:09","video":"","vorDoi":"10.1007/s11120-025-01187-w","vorDoiUrl":"https://doi.org/10.1007/s11120-025-01187-w","workflowStages":[]},"version":"v1","identity":"rs-7366542","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7366542","identity":"rs-7366542","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}