Establishment of tissue culture regeneration system of Phalaris arundinacea L. mature embryo

Establishment of tissue culture regeneration system of Phalaris arundinacea L. mature embryo | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Establishment of tissue culture regeneration system of Phalaris arundinacea L. mature embryo Siriguleng Bai This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4147065/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The present study used the mature embryos of two varieties of Phalaris arundinacea L. (Tongxuan No.1 and Chuancao No.3) as explants. These explants were treated with 70% ethanol for 12 minutes under sterile conditions, sterilized with 0.1% HgCl2 for 5 minutes, washed with distilled water, and inoculated on media containing different ratios of hormones to overcome the dormancy caused by seed pupae and seed coats and obtain the regenerated plants. The results showed that the total recovery rate of Tongxuan No.1 and Chuancao No.3 on the induction medium N6+3.0–4.0 mg/L 2,4-D reached 70%; Tongxuan No.1got the best differentiation rate of 95.38% on N6+0.5 mg/L 2,4-D+2.0 mg/L 6-BA medium. Chuancao No. 3 on N6+0.5 mg/L 2,4-D+4.0 mg/L 6-BA medium had the highest differentiation rate of 89.33%. Both varieties produced new regenerated plants. Phalaris arundinacea L. mature embryo tissue culture callus dormancy Full Text Additional Declarations No competing interests reported. Plate Ⅰ is available in the Supplementary Files section. Supplementary Files Figures.zip PlateⅠ The establishment of regeneration system of mature embryo of Phalaris arundinacea by tissue culture Note: (A) Embryo culture of 4 days; (B) embryo culture of 7 days; (C)subculture; (D)the green bud from callus; (E)stems and leaves formed by callus; (F)individual plants formed by differentiation; (G)the root system of regenerated plant; (H)the regenerated plant rawdata.xls Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4147065","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":291401394,"identity":"c648f6eb-2435-47a9-9e94-39e0b8896f55","order_by":0,"name":"Siriguleng Bai","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAArklEQVRIiWNgGAWjYDACZgaGA0BKjo29/QBpWoz5eM4kkGZZ4jwJBwPilMq38z48XJhTm94mwZDA8KNiG2EtBofZDQ7P3HY8t0268QBjz5nbRGhhZmM4zLvtWG6bzIEEZsY2IrTIN0O0pLNJJBgQp4XhMFhLTQLxWgwgWg4YtgED+SBRfpHvP8b8mXdbnbx8e/vBBz8qiHEY1Hlg8gDR6oGgjhTFo2AUjIJRMNIAAKFFOYYbyCgvAAAAAElFTkSuQmCC","orcid":"","institution":"Inner Mongolia Minzu University","correspondingAuthor":true,"prefix":"","firstName":"Siriguleng","middleName":"","lastName":"Bai","suffix":""}],"badges":[],"createdAt":"2024-03-22 04:52:06","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4147065/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4147065/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":55264939,"identity":"3b5f4501-bca2-439a-be25-ffcf12f673ee","added_by":"auto","created_at":"2024-04-25 01:51:28","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":446350,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4147065/v1_covered_81f508ec-db12-4868-9f51-8746cd1343c7.pdf"},{"id":54937021,"identity":"0a0cae30-5df4-43a3-ba40-ca728178b66b","added_by":"auto","created_at":"2024-04-18 22:21:35","extension":"zip","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":11802209,"visible":true,"origin":"","legend":"\u003cp\u003ePlateⅠ \u0026nbsp;The establishment of regeneration system of mature embryo of Phalaris arundinacea by tissue culture Note: (A) Embryo culture of 4 days; (B) embryo culture of 7 days; (C)subculture; (D)the green \u0026nbsp;bud from callus; (E)stems and leaves formed by callus; (F)individual plants formed by differentiation; (G)the root system of regenerated plant; (H)the regenerated plant\u003c/p\u003e","description":"","filename":"Figures.zip","url":"https://assets-eu.researchsquare.com/files/rs-4147065/v1/0ebcdc1fb4a2267733764ccb.zip"},{"id":54937020,"identity":"e89ebc58-647e-4a87-bd27-bc8c4dbaad2b","added_by":"auto","created_at":"2024-04-18 22:21:35","extension":"xls","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":37376,"visible":true,"origin":"","legend":"","description":"","filename":"rawdata.xls","url":"https://assets-eu.researchsquare.com/files/rs-4147065/v1/99cede9a61f84a95cb4d3558.xls"}],"financialInterests":"\u003cp\u003eNo competing interests reported.\u003c/p\u003e\n\u003cp\u003ePlate Ⅰ is available in the Supplementary Files section.\u003c/p\u003e","formattedTitle":"Establishment of tissue culture regeneration system of Phalaris arundinacea L. mature embryo","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Phalaris arundinacea L., mature embryo, tissue culture, callus, dormancy","lastPublishedDoi":"10.21203/rs.3.rs-4147065/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4147065/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"The present study used the mature embryos of two varieties of Phalaris arundinacea L. (Tongxuan No.1 and Chuancao No.3) as explants. These explants were treated with 70% ethanol for 12 minutes under sterile conditions, sterilized with 0.1% HgCl2 for 5 minutes, washed with distilled water, and inoculated on media containing different ratios of hormones to overcome the dormancy caused by seed pupae and seed coats and obtain the regenerated plants. The results showed that the total recovery rate of Tongxuan No.1 and Chuancao No.3 on the induction medium N6+3.0–4.0 mg/L 2,4-D reached 70%; Tongxuan No.1got the best differentiation rate of 95.38% on N6+0.5 mg/L 2,4-D+2.0 mg/L 6-BA medium. Chuancao No. 3 on N6+0.5 mg/L 2,4-D+4.0 mg/L 6-BA medium had the highest differentiation rate of 89.33%. Both varieties produced new regenerated plants.","manuscriptTitle":"Establishment of tissue culture regeneration system of Phalaris arundinacea L. mature embryo","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-04-18 22:21:30","doi":"10.21203/rs.3.rs-4147065/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"f94dfdbf-2d68-4bad-820c-8e3715ae34c2","owner":[],"postedDate":"April 18th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-04-22T18:16:33+00:00","versionOfRecord":[],"versionCreatedAt":"2024-04-18 22:21:30","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4147065","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4147065","identity":"rs-4147065","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}