Estimation and Validation of Geftinib by Rp- HPLC

Estimation and Validation of Geftinib by Rp- HPLC | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Estimation and Validation of Geftinib by Rp- HPLC Shaheen Sultana, M.Shravani, Maheshwari Kenchi, Dr.B.Ramu This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4123618/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract A simple, rapid, precise and accurate Rp-Hplc method was developed and validated for the determination of Geftinib citrate, in bulk and pharmaceutical dosage form. The separation is achieved on RP-HPLC using a PDA detector by incorporation of enpower 2 software with a flow rate of 1.0ml/min using a mixture of Methanol and water (15:85% v/v) as mobile phase. The column used was Hypersil C18 (4.6×150mm, 5µ) at a wave length of 284nm. The retention time of the Geftinib citrate was 3.515min.. The linearity of the drug was 25–125µg/m and the method precision for the determination of assay was below 2.0%RSD. The proposed method was validated and applied for the estimation of Geftinib in quality control of bulk and pharmaceutical dosage forms. Analytical Biochemistry Geftinib RP-HPLC validation Figures Figure 1 Figure 2 Figure 3 Introduction Geftinib citrate is the drug used for the treatment of specific type of cancer. It is a protease inhibitor for the treatment of multiple myloma .The IUPAC name of Geftinib is N 2 -(2,5-Dichlorobenzoyl)- N -[(1 R )-1-(dihydroxyboryl)-3-methylbutyl]glycinamide. Structure of Geftinib shown in Fig. 1 . Geftinib is a peptide analogue that reversibly inhibits the protein proteasome subunit beta type-5 (PSMB5), which is part of the 20Sproteasome complex. Its empirical formula is C14H19 BCl2N2O4. It is a white powder consists of molecular weight 361.03 and soluble in polar solvents like water, methanol etc. On November 20, 2015, the U.S. Food and Drug Administration approved Geftinib citrate for the treatment of multiple myeloma Geftinib is not official in any Pharmacopoeia. A literature survey on Geftinib revealed that, until now no analytical method was reported for its determination in bulk drug and its capsule dosage form.Since literature did not cite any method for determination of this drug from bulk drug as well as its formulation it was proposed to develop and validate a new method by using RP- HPLC method( 2 ). Materials and Methods The method development and validation was done by using waters Alliance 2895 seperation model with a PDA detector 996model.the data was acquired using Enpower 2 software and the column used was Hypersil C18, 150 x4.6mm,5 Geftinib is not official in any Pharmacopoeia. A literature survey on Geftinib revealed that, until now no analytical method was reported for its determination in bulk drug and its capsule dosage form.Since literature did not cite any method for determination of this drug from bulk drug as well as its formulation it was proposed to develop and validate a method by using RP- HPLC method( 2 ). Instrumentation and analytical conditions : The method development and validation was done by using Waters Alliance 2895 seperation model with a PDA detector 996model.The data was acquired using Enpower 2 software and the column used was Hypersil C18, 150 x4.6mm,5µ. Labman Digital Ultra sonicator was used for enhancing dissolution of the drug. A Lab india make pH meter was used for pH adjustment. Chemicals and Reagents: Pure Geftinib citrate gift sample was obtained from Sura Pharma Reaseach Lab ,Hyderbaded. HPLC grade water, Methanol-HPLC,Acetonitrile-HPLC from Merck ,Mumbai.. Labman Digital Ultra sonicator was used for enhancing dissolution of the drug. A Lab india make pH meter was used for pH adjustment. Preparation of Mobile phase ,Standard and Sample preparations: Preparation of Mobile phase: Accurately measure 150 ml (15%) of HPLC Methanol and 850 ml of HPLC Water (85%) were mixed and degassed with a digital ultrasonicater for 10 minutes and then filtered through 0.45 µ filter under vacuum filtration.The Mobile phase was used as the diluent. Preparation of Standard solution: Weigh accurately 10 mg of Geftinib citrate and transfer into a clean, dry 10ml volumetric flask and add 7ml of Methanol, sonicate to dissolve and removal of air completely and make the volume upto the mark with the diluent.Further pipette out 0.75ml of the above Geftinib stock solution into a 10ml volumetric flask and dilute up to the mark with diluent and filtered through 0.45µm membrane filter. Preparation of Sample solution: Each capsule was weighed and powered by using mortor and pestle and 10mg equivalent weight of Geftinibcitrate sample was weighed and transferred into 10ml of clean ,dry volumetric flask. Then add 7ml of diluent and sonicate to dissolve for 20min. The volume was made upto 10ml with diluent. Pipette out 0.75ml of the above solution into a 10ml volumetric flask and dilute up to the mark with diluent and filtered through 0.45µm membrane filter. Procedure: Inject the samples by changing the chromatographic conditions and record the chromatograms, note the conditions of proper peak elution for performing validation parameters as per ICH guidelines. Mobile Phase Optimization : Initially the mobile phase tried was Water, Methanol: Water and Acetonitrile: Water with varying proportions. Finally, the mobile phase was optimized to Methanol: Water in proportion 15:85 v/v respectively. Optimization of Column: The method was carried out with various C18 columns like Phenomenex column, Xterra, and Symmetry C18 column. Hypersil C18 (4.6 x 150mm, 5µm) was found to be ideal as it gave good peak shape and resolution at 1.0ml/min flow The sample was injected using 10 µl fixed loop and PDA detector was set at a wavelength of 284nm and the run time is 6min. Results and discussion The method developed was validated as per ICH guidelines. The system suitability parmeters are performed and other parameters like Accuracy,Presicion,Linearty,Robustness,LOD,LOQ are performed by using optimized chromatographic conditions. The Retention time of Geftinib citrate was 3.513min. The standard chromatograph are shown in Figure:2a. System Suitability: A standard solution was prepared by using Geftinib citrate stock solution as per test method and was injected for 5 times into HPLC column. From the system suitability studies it is observed that % RSD values were within the specified limit i.e not more than 2. System suitability data was presented in table:1 Specificity: It is the ability to asses unequivocally the analyte in the presence of components that may be expected to be present. Excipients that are commonly used were spiked into a pre weighed quantity of drugs. Appropriate dilutions were injected into chromatographic system and the quantities of the drugs were determined. results of the method are tabulated in table:2 Linearity: A series of solutions were prepared using Geftinib citrate working standard solution at a concentration levels from 25-125µg/ml of target concentration and the peak area response of all solutions are measured. A graph was plotted against the Concentration(µg/ml)on X-axis versus area/response on Y-axis. The detector response was found to be linear with a correlation coefficient of 0.999. linearity graph is shown in Figure:3 linearity results of the method are tabulated in table:3 Precision: Precision studies were performed (Method,Day to day). The results are reported in term of Relative standard deviation. The repeatability studies were carried out by estimsting response of 6 different concentrations of Geftinib citrate and reported in terms of % RSD, % RSD was 0.13, for method precision it was 0.11 and for day to day precision it was 0.12. precision results of the method are tabulated in table:4 Accuracy: Accuracy of the method was determined by calculating the recovery of Geftinib by the spiked method. Known quantity of Geftinib was added to a pre-determined sample solution and the amount of Geftinib was estimated by measuring peak areas. Mean % recovery values are within the limit(limit is 98–102%). Accuracy data was presented in table:5 &5a LOD and LOQ Limits: The level of detection(LOD) and level of Quantification (LOQ)were conducted on the basis of standard deviation of the response and the slope .The LOD and LOQ for Geftinib were found to be 5.2 and 15.8 respectively. Robustness: Robustness is a measure of its capacity to remain unaffected by small, but deliberated variation in the method parameters and gives an indication of its reliability during normal usage. Robustness of the method was studied by injecting 0.9 ml min-1,1.0 ml min-1,1.1ml min-1 and mobile ratio variation from more organic phase to less organic phase ratio. The method passed robustness test with well % RSD. .Robustness data was presented in table:6 Blank: Figure 2 : Chromatogram showing blank (mobile phase preparation) System suitability: Table 1 Results of system suitability for Geftinib S.No Peak Name RT Area Height (µV) USP Plate Count USP Tailing 1 Geftinib 3.513 2947505 275462 7462 1.1 2 Geftinib 3.516 2958475 275361 7462 1.1 3 Geftinib 3.515 2965847 275144 6472 1.1 4 Geftinib 3.517 2952642 275837 7183 1.1 5 Geftinib 3.512 2951645 275948 7428 1.1 Mean 2955223 Std. Dev. 7114.704 % RSD 0.24075 Specificity: Table 2 Peak results for Assay Standard S.No Name RT Area Height USP Tailing USP Plate Count 1 Geftinib 3.518 2967593 275837 1.1 6583 2 Geftinib 3.517 2967399 275922 1.1 5938 3 Geftinib 3.515 2960183 271844 1.1 5883 Table 2 a: Peak results for Assay sample S.No Name RT Area Height USP Tailing USP Plate Count 1 Geftinib 3.511 2983744 275833 1.1 7584 2 Geftinib 3.511 2958374 275984 1.1 6294 3 Geftinib 3.514 2957262 275481 1.1 8194 =2966460 / 2965058 × 10/75 × 75/0.0145 × 99.7/100 × 0.0174/12 × 100 =99.7 The % purity of Geftinib in pharmaceutical dosage form was found to be 99.7% Linearty: Table 3 For linearity data Conc. Level (%) Concentration(µg/ml) Average Peak Area 33 25 1083048 66 50 1973321 100 75 2955166 133 100 4063921 166 125 5006038 Precision Table 4 Results of repeatability for Geftinib: S. No Peak name RT Area(µV*sec) Height(µV) USP Plate Count USP Tailing 1 Geftinib 3.528 2958333 275983 7583 1.1 2 Geftinib 3.516 2951049 275911 7593 1.1 3 Geftinib 3.514 2959294 275955 8674 1.1 4 Geftinib 3.519 2953391 275921 7958 1.1 5 Geftinib 3.512 2950744 275221 9745 1.1 Mean 2954562 Std.dev 4028.083 %RSD 0.136334 Table 4 a: Results of Intermediate precision day1 for Geftinib S.No PeakName RT Area (µV*sec) Height (µV) USPPlate count USPTailing 1 Geftinib 3.517 2957344 275838 7194 1.1 2 Geftinib 3.514 2951847 275629 8573 1.1 3 Geftinib 3.517 2950834 276931 7655 1.1 4 Geftinib 3.517 2957155 275623 7944 1.1 5 Geftinib 3.512 2950185 275184 7562 1.1 6 Geftinib 3.518 2951750 275193 7585 1.1 Mean 2953186 Std. Dev. 3207.331 % RSD 0.108606 Table 4 b: Results of Intermediate precision Day 2 for Geftinib S.No PeakName RT Area (µV*sec) Height (µV) USPPlate count USPTailing 1 Geftinib 3.513 2951848 275929 7937 1.1 2 Geftinib 3.511 2958275 275222 7284 1.1 3 Geftinib 3.516 2950185 275857 7684 1.1 4 Geftinib 3.518 2957462 275163 7917 1.1 5 Geftinib 3.511 2957541 275164 7585 1.1 6 Geftinib 3.519 2951164 275154 7192 1.1 Mean 2954413 Std. Dev. 3715.025 % RSD 0.125745 Table 5 Results of Accuracy for concentration-50%, 100% and 150% S.No Conc. RT Area Height USP Tailing USP Plate Count 1 50% 3.519 1493763 275837 1.1 6453 2 50% 3.520 1493923 275819 1.1 7584 3 50% 3.519 1492575 275083 1.1 6785 4 100% 3.516 2983722 275829 1.1 7483 5 !00% 3.518 2984722 275143 1.1 6935 6 100% 3.519 2967382 275063 1.1 6285 7 150% 3.517 4462011 275622 1.1 7483 8 150% 3.519 4469483 275922 1.1 7265 9 150% 3.520 4489583 276331 1.1 7194 Table 5 a:The accuracy results for Geftinib %Concentration (at specification Level) Area Amount Added(ppm) Amount Found(ppm) %Recovery Mean Recovery 50% 1483420 37.5 36.96 98.5% 98.8% 100% 2978609 75 74.1 98.8% 150% 4473692 112.5 111.56 99.1% Robustness: Table 6 Results for Robustness Parameter used for sample analysis Peak Area RT Theoretical plates Tailing factor Actual Flow rate of 1.0 mL/min 2955764 3.513 7483 1.1 Less Flow rate of 0.9 mL/min 2958393 3.897 6028 1.1 More Flow rate of 1.1 mL/min 2956411 3.218 6928 1.2 Less organic phase 2950683 3.707 6733 1.2 More organic phase 2957265 3.350 6285 1.1 Conclusion In this study a simple, rapid,and reliable RP-HPLC method was developed and validated for the determination of Geftinib citrate in capsule dosage form. From the results obtained, we conclude that the suggested method showed high sensitivity, accuracy, and specificity. Moreover, this method was simple and economical and can be employed for the routine quality control of Geftinib in pharmaceutical dosage forms. Declarations CONFLICT OF INTEREST STATEMENT We declare that we have no conflict of interest. Acknowledgement: I am thankful to the Hetero Pharma labs,Hyderabad for providing gift sample of Geftinib and I am very thankful to the principal of kvk college of pharmacy and secretary of Kennedy education society,Hyd, for guidance, encouragement and providing laboratory facilities. References Dr. Kealey and P.J Haines, Analytical Chemistry, 1 st edition, Bios Publisher, (2002), PP 1-7. A.BraithWait and F.J.Smith, Chromatographic Methods, 5 th edition, Kluwer Academic Publisher, (1996), PP 1-2. Andrea Weston and Phyllisr. Brown, HPLC Principle and Practice, 1 st edition, Academic press, (1997), PP 24-37. Yuri Kazakevich and Rosario Lobrutto, HPLC for Pharmaceutical Scientists, 1 st edition, Wiley Interscience A JohnWiley & Sons, Inc., Publication, (2007), PP 15-23. Chromatography, (online). URL:http://en.wikipedia.org/wiki/Chromatography. Meyer V.R. Practical High-Performance Liquid Chromatography, 4 th Ed. England, John Wiley & Sons Ltd, (2004), PP 7-8. Sahajwalla CG a new drug development, vol 141, Marcel Dekker Inc., New York, (2004), PP 421–426. Introduction to Column. (Online),URL:http://amitpatel745.topcities.com/index_files/study/column care.pdf Detectors used in HPLC (online )URL:http://wiki.answers.com/Q/What_detectors_are_used_in_HPLC Detectors (online) ,URL:http://hplc.chem.shu.edu/NEW/HPLC_Book/Detectors/det_uvda.html URL:https://www.ninlaro.com/ URL:http://businesswireindia.com/news/news-details/us-fda-approves-takedas-ninlaro-Geftinib-first-only-oral-proteasome-inhibitor-treat-multiple-myeloma/46254 URL:www.medkoo.com URL:https://en.wikipedia.org/wiki/Geftinib. B. Ramu, Kaushal K. Chandrul, P. Shanmuga Pandiyan. Using 24 Factorial Designs optimization of Repaglinide Gastroretentive Drug Delivery System. Research J. Pharm. and Tech. 2021; 14(2):725-72 Gopikrishna, A.; Ramu, B.; Srikanth, G.; Rajkamal, B. Formulation of isoniazide sustained release formulation by using carbopol 934 P. Int. J. Appl. Pharm. Sci. Res. 2016, 1, 60–69. Ramu B, Saibaba SV. Role of community pharmacist in management of anaemia. Pharm Pharmacol Int J., 2018; 6(3): 216‒220. DOI: 10.15406/ppij.2018.06.00178 Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4123618","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":280927618,"identity":"934fcb86-2c29-405c-b6b1-a9663b129bba","order_by":0,"name":"Shaheen Sultana","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA3ElEQVRIiWNgGAWjYDACCQST8QGQ4OEjRQuzAUgLGyla2MBsglrMpZuffbpRcy9xu/ThY5Vfc+xk2BiYHz66gUeL5ZxjxrNzjhUbW/alpd2W3ZYMdBibsXEOHi0GNxKMmXPYEuQMzvCY3ZbcxgzUwsMmjV9L+mfmnH8JPCAtxZLb6onRkmPMnNsGsYXx47bDhLVYzsgpZs7tSzC27GFLlmbcdpyHjZmAX8wl0jcz53xLSNzOw3zw489t1fb87M0PH+N1GDKDmQfEYsajHEML4w8CqkfBKBgFo2BkAgDlYkBgRHUsfgAAAABJRU5ErkJggg==","orcid":"","institution":"K.v.k College of Pharmacy","correspondingAuthor":true,"prefix":"","firstName":"Shaheen","middleName":"","lastName":"Sultana","suffix":""},{"id":280927619,"identity":"9f63dab9-6480-4ed3-b572-2af0e2752e98","order_by":1,"name":"M.Shravani","email":"","orcid":"","institution":"K.V.K College of Pharmacy","correspondingAuthor":false,"prefix":"","firstName":"","middleName":"","lastName":"M.Shravani","suffix":""},{"id":280927620,"identity":"563f6606-6f88-4ebf-abc5-2851de30e614","order_by":2,"name":"Maheshwari Kenchi","email":"","orcid":"","institution":"K.V.K College of Pharmacy","correspondingAuthor":false,"prefix":"","firstName":"Maheshwari","middleName":"","lastName":"Kenchi","suffix":""},{"id":280927621,"identity":"dd08e420-c05e-4e25-a2f6-b9516f18f53f","order_by":3,"name":"Dr.B.Ramu","email":"","orcid":"https://orcid.org/0000-0002-1639-090X","institution":"K.V.K College of Pharmacy","correspondingAuthor":false,"prefix":"Dr.","firstName":"","middleName":"","lastName":"B.Ramu","suffix":""}],"badges":[],"createdAt":"2024-03-18 13:25:14","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-4123618/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4123618/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":53065725,"identity":"e3815452-d28f-4c9b-86c6-b50cf453d2f6","added_by":"auto","created_at":"2024-03-20 08:05:04","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":19952,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eGeftinib (1)\u003c/strong\u003e\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-4123618/v1/3dfaf4a33d62a86248c12030.png"},{"id":53065726,"identity":"33365ee4-9d6d-4726-a3e0-1e2bc961a340","added_by":"auto","created_at":"2024-03-20 08:05:04","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":41948,"visible":true,"origin":"","legend":"\u003cp\u003eChromatogram showing blank (mobile phase preparation)\u003c/p\u003e\n\u003cp\u003eFigure 2 a: Optimized Chromatogram (GeftinibStandard)\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-4123618/v1/f00da97dc73eef1292102c3f.png"},{"id":53065724,"identity":"77c3d1d1-e7fb-4863-9af0-22fe6a5775d6","added_by":"auto","created_at":"2024-03-20 08:05:04","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":43724,"visible":true,"origin":"","legend":"\u003cp\u003eLegend not included with this version.\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-4123618/v1/2681e2c1cf8940022710c41f.png"},{"id":53066841,"identity":"d3e66871-ad8e-43c3-888c-cf50b08b84c5","added_by":"auto","created_at":"2024-03-20 08:13:08","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":555032,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4123618/v1/9d1f5d2c-5932-4c39-ab5a-257d1816e021.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eEstimation and Validation of Geftinib by Rp- HPLC\u003c/strong\u003e\u003c/p\u003e","fulltext":[{"header":"Introduction","content":"\u003cp\u003eGeftinib citrate is the drug used for the treatment of specific type of cancer. It is a protease inhibitor for the treatment of multiple myloma .The IUPAC name of Geftinib is \u003cem\u003eN\u003c/em\u003e\u003csup\u003e2\u003c/sup\u003e-(2,5-Dichlorobenzoyl)-\u003cem\u003eN\u003c/em\u003e-[(1\u003cem\u003eR\u003c/em\u003e)-1-(dihydroxyboryl)-3-methylbutyl]glycinamide. Structure of Geftinib shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e. Geftinib is a peptide analogue that reversibly inhibits the protein proteasome subunit beta type-5 (PSMB5), which is part of the 20Sproteasome complex. Its empirical formula is C14H19 BCl2N2O4. It is a white powder consists of molecular weight 361.03 and soluble in polar solvents like water, methanol etc.\u003c/p\u003e \u003cp\u003eOn November 20, 2015, the U.S. Food and Drug Administration approved Geftinib citrate for the treatment of multiple myeloma\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eGeftinib is not official in any Pharmacopoeia. A literature survey on Geftinib revealed that, until now no analytical method was reported for its determination in bulk drug and its capsule dosage form.Since literature did not cite any method for determination of this drug from bulk drug as well as its formulation it was proposed to develop and validate a new method by using RP-\u003cb\u003eHPLC\u003c/b\u003e method(\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e).\u003c/p\u003e"},{"header":"Materials and Methods","content":"\u003cp\u003eThe method development and validation was done by using waters Alliance 2895 seperation model with a PDA detector 996model.the data was acquired using Enpower 2 software and the column used was Hypersil C18, 150 x4.6mm,5\u003c/p\u003e \u003cp\u003eGeftinib is not official in any Pharmacopoeia. A literature survey on Geftinib revealed that, until now no analytical method was reported for its determination in bulk drug and its capsule dosage form.Since literature did not cite any method for determination of this drug from bulk drug as well as its formulation it was proposed to develop and validate a method by using RP-\u003cb\u003eHPLC\u003c/b\u003e method(\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e).\u003c/p\u003e \u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003e\u003cb\u003eInstrumentation and analytical conditions\u003c/b\u003e:\u003c/h2\u003e \u003cp\u003eThe method development and validation was done by using Waters Alliance 2895 seperation model with a PDA detector 996model.The data was acquired using Enpower 2 software and the column used was Hypersil C18, 150 x4.6mm,5\u0026micro;. Labman Digital Ultra sonicator was used for enhancing dissolution of the drug. A Lab india make pH meter was used for pH adjustment.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec4\" class=\"Section2\"\u003e \u003ch2\u003eChemicals and Reagents:\u003c/h2\u003e \u003cp\u003ePure Geftinib citrate gift sample was obtained from Sura Pharma Reaseach Lab ,Hyderbaded. HPLC grade water, Methanol-HPLC,Acetonitrile-HPLC from Merck ,Mumbai.. Labman Digital Ultra sonicator was used for enhancing dissolution of the drug. A Lab india make pH meter was used for pH adjustment.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec5\" class=\"Section2\"\u003e \u003ch2\u003ePreparation of Mobile phase ,Standard and Sample preparations:\u003c/h2\u003e \u003cdiv id=\"Sec6\" class=\"Section3\"\u003e \u003ch2\u003ePreparation of Mobile phase:\u003c/h2\u003e \u003cp\u003eAccurately measure 150 ml (15%) of HPLC Methanol and 850 ml of HPLC Water (85%) were mixed and degassed with a digital ultrasonicater for 10 minutes and then filtered through 0.45 \u0026micro; filter under vacuum filtration.The Mobile phase was used as the diluent.\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv id=\"Sec7\" class=\"Section2\"\u003e \u003ch2\u003ePreparation of Standard solution:\u003c/h2\u003e \u003cp\u003eWeigh accurately 10 mg of Geftinib citrate and transfer into a clean, dry 10ml volumetric flask and add 7ml of Methanol, sonicate to dissolve and removal of air completely and make the volume upto the mark with the diluent.Further pipette out 0.75ml of the above Geftinib stock solution into a 10ml volumetric flask and dilute up to the mark with diluent and filtered through 0.45\u0026micro;m membrane filter.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003ePreparation of Sample solution:\u003c/h2\u003e \u003cp\u003eEach capsule was weighed and powered by using mortor and pestle and 10mg equivalent weight of Geftinibcitrate sample was weighed and transferred into 10ml of clean ,dry volumetric flask. Then add 7ml of diluent and sonicate to dissolve for 20min. The volume was made upto 10ml with diluent.\u003c/p\u003e \u003cp\u003ePipette out 0.75ml of the above solution into a 10ml volumetric flask and dilute up to the mark with diluent and filtered through 0.45\u0026micro;m membrane filter.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec9\" class=\"Section2\"\u003e \u003ch2\u003eProcedure:\u003c/h2\u003e \u003cp\u003eInject the samples by changing the chromatographic conditions and record the chromatograms, note the conditions of proper peak elution for performing validation parameters as per ICH guidelines.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec10\" class=\"Section2\"\u003e \u003ch2\u003e\u003cb\u003eMobile Phase Optimization\u003c/b\u003e:\u003c/h2\u003e \u003cp\u003eInitially the mobile phase tried was Water, Methanol: Water and Acetonitrile: Water with varying proportions. Finally, the mobile phase was optimized to Methanol: Water in proportion 15:85 v/v respectively.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003eOptimization of Column:\u003c/h2\u003e \u003cp\u003eThe method was carried out with various C18 columns like Phenomenex column, Xterra, and Symmetry C18 column. Hypersil C18 (4.6 x 150mm, 5\u0026micro;m) was found to be ideal as it gave good peak shape and resolution at 1.0ml/min flow The sample was injected using 10 \u0026micro;l fixed loop and PDA detector was set at a wavelength of 284nm and the run time is 6min.\u003c/p\u003e \u003c/div\u003e"},{"header":"Results and discussion","content":"\u003cp\u003eThe method developed was validated as per ICH guidelines. The system suitability parmeters are performed and other parameters like Accuracy,Presicion,Linearty,Robustness,LOD,LOQ are performed by using optimized chromatographic conditions. The Retention time of Geftinib citrate was 3.513min. The standard chromatograph are shown in Figure:2a.\u003c/p\u003e \u003cdiv id=\"Sec13\" class=\"Section2\"\u003e \u003ch2\u003eSystem Suitability:\u003c/h2\u003e \u003cp\u003eA standard solution was prepared by using Geftinib citrate stock solution as per test method and was injected for 5 times into HPLC column. From the system suitability studies it is observed that % RSD values were within the specified limit i.e not more than 2. System suitability data was presented in table:1\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec14\" class=\"Section2\"\u003e \u003ch2\u003eSpecificity:\u003c/h2\u003e \u003cp\u003eIt is the ability to asses unequivocally the analyte in the presence of components that may be expected to be present. Excipients that are commonly used were spiked into a pre weighed quantity of drugs. Appropriate dilutions were injected into chromatographic system and the quantities of the drugs were determined. results of the method are tabulated in table:2\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec15\" class=\"Section2\"\u003e \u003ch2\u003eLinearity:\u003c/h2\u003e \u003cp\u003eA series of solutions were prepared using Geftinib citrate working standard solution at \u003cem\u003ea\u003c/em\u003e concentration levels from 25-125\u0026micro;g/ml of target concentration and the peak area response of all solutions are measured. A graph was plotted against the Concentration(\u0026micro;g/ml)on X-axis versus area/response on Y-axis. The detector response was found to be linear with a correlation coefficient of 0.999. linearity graph is shown in Figure:3 linearity results of the method are tabulated in table:3\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec16\" class=\"Section2\"\u003e \u003ch2\u003ePrecision:\u003c/h2\u003e \u003cp\u003ePrecision studies were performed (Method,Day to day). The results are reported in term of Relative standard deviation. The repeatability studies were carried out by estimsting response of 6 different concentrations of Geftinib citrate and reported in terms of % RSD, % RSD was 0.13, for method precision it was 0.11 and for day to day precision it was 0.12. precision results of the method are tabulated in table:4\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec17\" class=\"Section2\"\u003e \u003ch2\u003eAccuracy:\u003c/h2\u003e \u003cp\u003eAccuracy of the method was determined by calculating the recovery of Geftinib by the spiked method. Known quantity of Geftinib was added to a pre-determined sample solution and the amount of Geftinib was estimated by measuring peak areas. Mean % recovery values are within the limit(limit is 98\u0026ndash;102%). Accuracy data was presented in table:5 \u0026amp;5a\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec18\" class=\"Section2\"\u003e \u003ch2\u003eLOD and LOQ Limits:\u003c/h2\u003e \u003cp\u003eThe level of detection(LOD) and level of Quantification (LOQ)were conducted on the basis of standard deviation of the response and the slope .The LOD and LOQ for Geftinib were found to be 5.2 and 15.8 respectively.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec19\" class=\"Section2\"\u003e \u003ch2\u003eRobustness:\u003c/h2\u003e \u003cp\u003eRobustness is a measure of its capacity to remain unaffected by small, but deliberated variation in the method parameters and gives an indication of its reliability during normal usage. Robustness of the method was studied by injecting 0.9 ml min-1,1.0 ml min-1,1.1ml min-1 and mobile ratio variation from more organic phase to less organic phase ratio. The method passed robustness test with well % RSD. .Robustness data was presented in table:6\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec20\" class=\"Section2\"\u003e \u003ch2\u003eBlank:\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e: Chromatogram showing blank (mobile phase preparation)\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec21\" class=\"Section2\"\u003e \u003ch2\u003eSystem suitability:\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eResults of system suitability for Geftinib\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eS.No\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePeak Name\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eRT\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eArea\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eHeight (\u0026micro;V)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eUSP Plate Count\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c7\"\u003e \u003cp\u003eUSP Tailing\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.513\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2947505\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275462\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7462\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.516\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2958475\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275361\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7462\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.515\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2965847\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275144\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e6472\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.517\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2952642\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275837\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7183\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.512\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2951645\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275948\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7428\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eMean\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2955223\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eStd. Dev.\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e7114.704\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003e% RSD\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.24075\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec22\" class=\"Section2\"\u003e \u003ch2\u003eSpecificity:\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003ePeak results for Assay Standard\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eS.No\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eName\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eRT\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eArea\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eHeight\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eUSP Tailing\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c7\"\u003e \u003cp\u003eUSP Plate Count\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.518\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2967593\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275837\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6583\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.517\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2967399\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275922\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e5938\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.515\u003c/p\u003e \u003c/td\u003e \u003ctd 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\" width=\"599\" height=\"83\"\u003e\u003c/p\u003e\n\u003cp\u003e=2966460 / 2965058 \u0026times; 10/75 \u0026times; 75/0.0145 \u0026times; 99.7/100 \u0026times; 0.0174/12 \u0026times; 100\u003c/p\u003e\n\u003cp\u003e=99.7\u003c/p\u003e\u003cdiv id=\"Sec25\" class=\"Section4\"\u003e \u003cp\u003eThe % purity of Geftinib in pharmaceutical dosage form was found to be 99.7%\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv id=\"Sec26\" class=\"Section3\"\u003e \u003ch2\u003eLinearty:\u003c/h2\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab4\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eFor linearity data\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"3\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eConc. Level (%)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eConcentration(\u0026micro;g/ml)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eAverage Peak Area\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e1083048\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e66\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e50\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e1973321\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e100\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e75\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2955166\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e133\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e100\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e4063921\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e166\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e125\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5006038\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv id=\"Sec27\" class=\"Section2\"\u003e \u003ch2\u003ePrecision\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab5\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eResults of repeatability for Geftinib:\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eS. No\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePeak name\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eRT\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eArea(\u0026micro;V*sec)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eHeight(\u0026micro;V)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eUSP Plate Count\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c7\"\u003e \u003cp\u003eUSP Tailing\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.528\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2958333\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275983\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7583\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.516\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2951049\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275911\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7593\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.514\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2959294\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275955\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e8674\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.519\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2953391\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275921\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7958\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.512\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2950744\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275221\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e9745\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eMean\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2954562\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eStd.dev\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e4028.083\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003e%RSD\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.136334\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab6\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003ea: Results of Intermediate precision day1 for Geftinib\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eS.No\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePeakName\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eRT\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eArea (\u0026micro;V*sec)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eHeight (\u0026micro;V)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eUSPPlate count\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c7\"\u003e \u003cp\u003eUSPTailing\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.517\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2957344\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275838\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7194\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.514\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2951847\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275629\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e8573\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.517\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2950834\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e276931\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7655\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.517\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2957155\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275623\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7944\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.512\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2950185\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275184\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7562\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.518\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2951750\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275193\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7585\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eMean\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2953186\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eStd. Dev.\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e3207.331\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003e% RSD\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.108606\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab7\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eb: Results of Intermediate precision Day 2 for Geftinib\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eS.No\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePeakName\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eRT\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eArea (\u0026micro;V*sec)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eHeight (\u0026micro;V)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eUSPPlate count\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c7\"\u003e \u003cp\u003eUSPTailing\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.513\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2951848\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275929\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7937\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.511\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2958275\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275222\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7284\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.516\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2950185\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275857\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7684\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.518\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2957462\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275163\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7917\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.511\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2957541\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275164\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7585\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGeftinib\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.519\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2951164\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275154\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7192\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eMean\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2954413\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eStd. Dev.\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e3715.025\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003e% RSD\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.125745\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab8\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 5\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eResults of Accuracy for concentration-50%, 100% and 150%\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eS.No\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eConc.\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eRT\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eArea\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eHeight\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eUSP Tailing\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c7\"\u003e \u003cp\u003eUSP Plate Count\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e50%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.519\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e1493763\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275837\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6453\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e50%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.520\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e1493923\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275819\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e7584\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e50%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.519\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e1492575\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275083\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6785\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e100%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.516\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2983722\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275829\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e7483\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e!00%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.518\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2984722\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275143\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6935\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e100%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.519\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2967382\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275063\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6285\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e150%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.517\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e4462011\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275622\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e7483\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e150%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.519\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e4469483\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e275922\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e7265\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e150%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.520\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e4489583\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e276331\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e7194\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab9\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 5\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003ea:The accuracy results for Geftinib\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003e%Concentration (at specification Level)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eArea\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eAmount Added(ppm)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eAmount Found(ppm)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003e%Recovery\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eMean Recovery\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e50%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e1483420\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e37.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e36.96\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e98.5%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\" morerows=\"2\" rowspan=\"3\"\u003e \u003cp\u003e98.8%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e100%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e2978609\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e75\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e74.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e98.8%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e150%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e4473692\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e112.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e111.56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e99.1%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec28\" class=\"Section2\"\u003e \u003ch2\u003eRobustness:\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab10\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 6\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eResults for Robustness\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"5\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eParameter used for sample analysis\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePeak Area\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eRT\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eTheoretical plates\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eTailing factor\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eActual Flow rate of 1.0 mL/min\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e2955764\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.513\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7483\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eLess Flow rate of 0.9 mL/min\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e2958393\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.897\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6028\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMore Flow rate of 1.1 mL/min\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e2956411\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.218\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6928\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e1.2\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eLess organic phase\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e2950683\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.707\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6733\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e1.2\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMore organic phase\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e2957265\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.350\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6285\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e1.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e"},{"header":"Conclusion","content":"\u003cp\u003eIn this study a simple, rapid,and reliable RP-HPLC method was developed and validated for the determination of Geftinib citrate in capsule dosage form. From the results obtained, we conclude that the suggested method showed high sensitivity, accuracy, and specificity. Moreover, this method was simple and economical and can be employed for the routine quality control of Geftinib in pharmaceutical dosage forms.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eCONFLICT OF INTEREST STATEMENT\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe declare that we have no conflict of interest.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgement:\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eI am thankful to the Hetero \u0026nbsp;Pharma labs,Hyderabad for providing gift sample of Geftinib \u0026nbsp;and\u003c/p\u003e\n\u003cp\u003eI am very thankful to the principal of \u0026nbsp;kvk college of pharmacy and secretary of \u0026nbsp;Kennedy education society,Hyd, for guidance, encouragement and providing laboratory facilities.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eDr. Kealey and P.J Haines, Analytical Chemistry, 1\u003csup\u003est\u003c/sup\u003eedition, Bios Publisher, (2002), PP 1-7.\u003c/li\u003e\n\u003cli\u003eA.BraithWait and F.J.Smith, Chromatographic Methods, 5\u003csup\u003eth\u003c/sup\u003eedition, Kluwer Academic Publisher, (1996), PP 1-2.\u003c/li\u003e\n\u003cli\u003eAndrea Weston and Phyllisr. Brown, HPLC Principle and Practice, 1\u003csup\u003est \u003c/sup\u003eedition, Academic press, (1997), PP 24-37.\u003c/li\u003e\n\u003cli\u003eYuri Kazakevich and Rosario Lobrutto, HPLC for Pharmaceutical Scientists, 1\u003csup\u003est\u003c/sup\u003eedition, Wiley Interscience A JohnWiley \u0026amp; Sons, Inc., Publication, (2007), PP 15-23.\u003c/li\u003e\n\u003cli\u003eChromatography, (online). URL:http://en.wikipedia.org/wiki/Chromatography. \u003c/li\u003e\n\u003cli\u003eMeyer V.R. Practical High-Performance Liquid Chromatography, 4\u003csup\u003eth \u003c/sup\u003eEd. England, John Wiley \u0026amp; Sons Ltd, (2004), PP 7-8.\u003c/li\u003e\n\u003cli\u003eSahajwalla CG a new drug development, vol 141, Marcel Dekker Inc., New York, (2004), PP 421\u0026ndash;426.\u003c/li\u003e\n\u003cli\u003eIntroduction to Column. (Online),URL:http://amitpatel745.topcities.com/index_files/study/column care.pdf\u003c/li\u003e\n\u003cli\u003eDetectors used in HPLC (online )URL:http://wiki.answers.com/Q/What_detectors_are_used_in_HPLC\u003c/li\u003e\n\u003cli\u003eDetectors (online) ,URL:http://hplc.chem.shu.edu/NEW/HPLC_Book/Detectors/det_uvda.html\u003c/li\u003e\n\u003cli\u003eURL:https://www.ninlaro.com/\u003c/li\u003e\n\u003cli\u003eURL:http://businesswireindia.com/news/news-details/us-fda-approves-takedas-ninlaro-Geftinib-first-only-oral-proteasome-inhibitor-treat-multiple-myeloma/46254\u003c/li\u003e\n\u003cli\u003eURL:www.medkoo.com\u003c/li\u003e\n\u003cli\u003eURL:https://en.wikipedia.org/wiki/Geftinib.\u003c/li\u003e\n\u003cli\u003eB. Ramu, Kaushal K. Chandrul, P. Shanmuga Pandiyan. Using 24 Factorial Designs optimization of Repaglinide Gastroretentive Drug Delivery System. Research J. Pharm. and Tech. 2021; 14(2):725-72\u003c/li\u003e\n\u003cli\u003eGopikrishna, A.; Ramu, B.; Srikanth, G.; Rajkamal, B. Formulation of isoniazide sustained release formulation by using carbopol 934 P. Int. J. Appl. Pharm. Sci. Res. 2016, 1, 60\u0026ndash;69.\u003c/li\u003e\n\u003cli\u003eRamu B, Saibaba SV. Role of community pharmacist in management of anaemia. Pharm Pharmacol Int J., 2018; 6(3): 216‒220. DOI: 10.15406/ppij.2018.06.00178\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"K.V.K COLLEGE OF PHARMACY SURMAIGUDA HYDERABAD","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Geftinib, RP-HPLC, validation","lastPublishedDoi":"10.21203/rs.3.rs-4123618/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4123618/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eA simple, rapid, precise and accurate Rp-Hplc method was developed and validated for the determination of Geftinib citrate, in bulk and pharmaceutical dosage form. The separation is achieved on RP-HPLC using a PDA detector by incorporation of enpower 2 software with a flow rate of 1.0ml/min using a mixture of Methanol and water (15:85% v/v) as mobile phase. The column used was Hypersil C18 (4.6×150mm, 5µ) at a wave length of 284nm. The retention time of the Geftinib citrate was 3.515min.. The linearity of the drug was 25–125µg/m and the method precision for the determination of assay was below 2.0%RSD.\u003c/p\u003e\n\u003cp\u003eThe proposed method was validated and applied for the estimation of Geftinib in quality control of bulk and pharmaceutical dosage forms.\u003c/p\u003e","manuscriptTitle":"Estimation and Validation of Geftinib by Rp- HPLC","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-03-20 08:05:00","doi":"10.21203/rs.3.rs-4123618/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"07ca7d03-ffc7-4241-83cc-331b01d05a32","owner":[],"postedDate":"March 20th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":29580940,"name":"Analytical Biochemistry"}],"tags":[],"updatedAt":"2024-03-20T08:05:00+00:00","versionOfRecord":[],"versionCreatedAt":"2024-03-20 08:05:00","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4123618","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4123618","identity":"rs-4123618","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}